49 research outputs found
Corrigendum
Evaluation of genetic diversity: Which of the varieties can be named ,Rebula' (Vitis vinifera L.)?Vitis 49 (4), 129-136 (2010
Evaluation of genetic diversity: Which o f the v arieties can be named 'Rebula' (Vitis vinifera L.)?
The variety 'Rebula' is an indigenous but also ancient variety in the Slovenian and Italian world-renowned winegrowing district Collio dating back to the ages of the Roman Empire. The DNA analyses of the variety ‘Rebula’ and its potential related varieties were conducted to evaluate its biodiversity. The variety 'Rebula' showed a very low similarity (16 %) with other analysed varieties, also with 'Rebula briška' and 'Rebula-old'. The varieties called 'Prosecco', 'Števerjana', 'Beli teran' and 'Briška Glera' revealed identical genotypes in all 11 SSR microsatellites analysed, therefore are regarded as synonyms. The varieties 'Rebula' and 'Ribolla gialla' revealed the identical SSR profile at 8 out of 9 SSR loci. 'Vitovska Grganja' share with 'Vitouska' only 55 % of analysed alleles but show their first degree relationship. The DNA analysis still showed existing deviations in synonyms and homonyms linked to the variety 'Rebula' what impedes its standardisation and revitalization.
Genetic clustering and parentage analysis of Western Balkan grapevines (Vitis vinifera L.)
A total of 90 grapevine samples collected in five countries of the Western Balkan region were evaluated for trueness-to-type and kinship relations based on comparative analysis with 1,130 grapevine genotypes held at the INRA "Domaine de Vassal" French Grape Germplasm Repository, using 14 microsatellite markers. In the context of the comparative analysis, twenty-four synonyms/counterparts and the putative parents for twelve Balkan accessions were identified. We discovered five pairs of homonyms, subsequently confirming the identity or parentage of three of them. Some of the examined accessions were identified either on the basis of the genotypes found in the literature, or through parentage relationships revealed in this study. For the remaining fifty accessions we were unable to establish either their pedigree or to identify them on the basis of SSR profiles available elsewhere. Finally, the Balkan genotypes that were not well classified by synonymy or parentage analysis were further studied with a Principal Coordinate Analysis to reveal genetic clustering within larger datasets of genotypes. The graphical display of the individual and group distances showed that about forty accessions (85 %) are structured within a group of Balkan and Eastern Europe genotypes and only a minor proportion resulted in admixed population assignment
Measurement of polarization-transfer to bound protons in carbon and its virtuality dependence
We measured the ratio of the transverse to longitudinal
components of polarization transferred from electrons to bound protons in
by the process at the
Mainz Microtron (MAMI). We observed consistent deviations from unity of this
ratio normalized to the free-proton ratio,
, for both -
and -shell knocked out protons, even though they are embedded in averaged
local densities that differ by about a factor of two. The dependence of the
double ratio on proton virtuality is similar to the one for knocked out protons
from and , suggesting a universal behavior.
It further implies no dependence on average local nuclear density
Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate
Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P lt 0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup.This is the peer-reviewed version of the article: Guegan, H.; Štajner, T.; Bobić, B.; Press, C.; Olariu, R. T.; Olson, K.; Srbljanović, J.; Montoya, J. G.; Đurković-Đaković, O.; Robert-Gangneux, F. Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. Journal of Clinical Microbiology 2021, 59 (2). [https://doi.org/10.1128/JCM.01368-20
Comparison of recoil polarization in the process for protons extracted from and shell
We present first measurements of the double ratio of the polarization
transfer components for knock-out protons from and
shells in measured by the reaction in quasi-elastic kinematics. The data are
compared to theoretical predictions in relativistic distorted-wave impulse
approximation. Our results show that differences between - and -shell
protons, observed when compared at the same initial momentum (missing momentum)
largely disappear when the comparison is done at the same proton virtuality. We
observe no density-dependent medium modifications for protons from and
shells with the same virtuality in spite of the large differences in the
respective nuclear densities
Beam-Recoil Polarization Measurement of π0 Electroproduction on the Proton in the Region of the Roper Resonance
The helicity-dependent recoil proton polarizations P[superscript '][subscript x] and P[superscript ′][subscript z] as well as the helicity-independent component P[subscript y] have been measured in the p([→ over e],e[superscript ′][→ over p])π[superscript 0] reaction at four-momentum transfer Q[superscript 2]≃0.1 GeV[superscript 2], center-of-mass proton emission angle θ[superscript *][subscript p]≃90°, and invariant mass W≃1440 MeV. This first precise measurement of double-polarization observables in the energy domain of the Roper resonance P[subscript 11](1440) by exploiting recoil polarimetry has allowed for the extraction of its scalar electroexcitation amplitude at an unprecedentedly low value of Q[superscript 2], establishing a powerful instrument for probing the interplay of quark and meson degrees of freedom in the nucleon
Nuclear density dependence of polarization transfer in quasi-elastic reactions
The ratio of the transverse and longitudinal component of polarization
transfer to protons in quasi-elastic
reaction, , is sensitive to the proton's
electromagnetic form factor ratio, . To explore density-dependent
in-medium modifications, a comparison of polarization transfer ratios involving
protons from distinct nuclear shells, each with different local nuclear
densities, has been proposed. In this study, we present such comparisons
between four shells, , in and
, in . In an effort to account for other
many-body effects that may differ between shells, we use state-of-the-art
relativistic distorted-wave impulse-approximation (RDWIA) calculation and
present the double ratios, as well as the super ratios,
, for chosen shells A
and B, as a function of effective local nuclear densities. We find that double
ratios for individual shells show a dependence on the probed effective nuclear
densities. Studying the ratios, we observed a systematic variation between
pairs of higher- and lower-density shells