Tumour necrosis factor-α and adenosine in endotoxin shockleading related cardiovascular symptoms

We have observed uncontrollable cardiogenic shock as a cardiovascular manifestation of systemic inflammatory response syndrome (SIRS) leading to death in a 62-year-old woman. The diagnosis of SIRS was based on the demonstration of endotoxinaemia, and highly elevated plasma levels of tumour necrosis factor (TNF)-α, and interleukin (IL)-10. We suggest that these cytokines may contribute to the terminal SIRS-related arrythmias, impaired myocardial contractility, as well as increased vascular permeability. In addition, the increased production of adenosine, a counter-regulatory mediator of inflammation, may also play a role in cardiodepression. We suggest a relationship between the action of TNF-α , IL-10 and adenosine in the pathogenesis of circulatory symptoms described above

Experimental determination of the 3^3He(α\alpha,γ\gamma)7^7Be reaction cross section above the 7^7Be proton separation threshold

The $^3$He($\alpha$,$\gamma$)$^7$Be reaction plays a major role both in the BBN producing the majority of the primordial $^7$Li, and in the pp-chain, where it is the branching point. As a few-nucleon system, this reaction is often used to validate ab-initio theoretical calculations and/or test R-matrix theory and code implementations. For the latter, experimental data in an extended energy range is of crucial importance to test the fit and extrapolation capabilities of the different codes. The $^3$He($\alpha$,$\gamma$)$^7$Be reaction cross section has been measured by several groups up to the first resonance ($E_{c.m.} \approx 3$ MeV) in the reaction. However, only one dataset exists above the $^7$Be proton separation threshold measured in a narrow energy range ($E_{c.m.} = 4.0-4.4$ MeV). In this work we extend the available experimental capture cross section database to the energy range of known $^7$Be levels. The activation method was used. The experiment was performed using a thin-window gas cell with two high-purity Al foils as entrance and exit windows. The activity of the $^7$Be nuclei implanted in the exit/catcher foil was measured by detecting the yield of the emitted $\gamma$~rays using shielded high-purity germanium detectors. New experimental $^3$He($\alpha$,$\gamma$)$^7$Be reaction cross section data were obtained for the first time in the $E_{c.m.}=4.3-8.3$ MeV energy region. The new dataset with about 0.2 MeV step covers the energy range of known levels and particle separation thresholds. No prominent structures are observer around the $^7$Be levels. The reaction cross section is slowly increasing with increasing energy. Above the $^6$Li$+p_1$ threshold, a decrease starts in the cross section trend. The overall structure of the cross section suggest a broad resonance peaking around $E_x=7.5$ MeV $^7$Be excitation energy, with a width of 8 MeV.Comment: Accepted for publication in PR

Cross section of 3He(α, γ)7Be around the 7Be proton separation threshold

Background: The 3He(α, γ )7Be reaction is a widely studied nuclear reaction; however, it is still not understood with the required precision. It has a great importance both in Big Bang nucleosynthesis and in solar hydrogen burning. The low mass number of the reaction partners makes it also suitable for testing microscopic calculations. Purpose: Despite the high number of experimental studies, none of them addresses the 3He(α, γ )7Be reaction cross sections above 3.1-MeV center-of-mass energy. Recently, a previously unobserved resonance in the 6Li(p, γ )7Be reaction suggested a new level in 7Be, which would also have an impact on the 3He(α, γ )7Be reaction in the energy range above 4.0 MeV. The aim of the present experiment is to measure the 3He(α, γ )7Be reaction cross section in the energy range of the proposed level. Method: For this investigation the activation technique was used. A thin window gas-cell target confining 3He gas was irradiated using an α beam. The 7Be produced was implanted into the exit foil. The 7Be activity was determined by counting the γ rays following its decay by a well-shielded high-purity germanium detector. Results: Reaction cross sections have been determined between Ecm = 4.0 and 4.4 MeV with 0.04-MeV steps covering the energy range of the proposed nuclear level. One lower-energy cross-section point was also determined to be able to compare the results with previous studies. Conclusions: A constant cross section of around 10.5 μb was observed around the 7Be proton separation energy. An upper limit of 45 neV for the strength of a 3He(α, γ )7Be resonance is derived

Exploring the circumstellar environment of the young eruptive star V2492 Cyg

Context. V2492 Cyg is a young eruptive star that went into outburst in 2010. The near-infrared color changes observed since the outburst peak suggest that the source belongs to a newly defined sub-class of young eruptive stars, where time-dependent accretion and variable line-of-sight extinction play a combined role in the flux changes. Aims. In order to learn about the origin of the light variations and to explore the circumstellar and interstellar environment of V2492 Cyg, we monitored the source at ten different wavelengths, between 0.55 \mu m and 2.2 \mu m from the ground and between 3.6 \mu m and 160 \mu m from space. Methods. We analyze the light curves and study the color-color diagrams via comparison with the standard reddening path. We examine the structure of the molecular cloud hosting V2492 Cyg by computing temperature and optical depth maps from the far-infrared data. Results. We find that the shapes of the light curves at different wavelengths are strictly self-similar and that the observed variability is related to a single physical process, most likely variable extinction. We suggest that the central source is episodically occulted by a dense dust cloud in the inner disk, and, based on the invariability of the far-infrared fluxes, we propose that it is a long-lived rather than a transient structure. In some respects, V2492 Cyg can be regarded as a young, embedded analog of UX Orionis-type stars. Conclusions. The example of V2492 Cyg demonstrates that the light variations of young eruptive stars are not exclusively related to changing accretion. The variability provided information on an azimuthally asymmetric structural element in the inner disk. Such an asymmetric density distribution in the terrestrial zone may also have consequences for the initial conditions of planet formation.Comment: 9 pages, 7 figures, 2 online tables, accepted for publication in A&

Inner disc rearrangement revealed by dramatic brightness variations in the young star PV Cep

Young Sun-like stars at the beginning of the pre-main sequence (PMS) evolution are surrounded by accretion discs and remnant protostellar envelopes. Photometric and spectroscopic variations of these stars are driven by interactions of the star with the disc. Time scales and wavelength dependence of the variability carry information on the physical mechanisms behind these interactions. We conducted multi-epoch, multi-wavelength study of PV Cep, a strongly variable, accreting PMS star. By combining our own observations from 2004-2010 with archival and literature data, we show that PV Cep started a spectacular fading in 2005, reaching an I_C-band amplitude of 4 mag. Analysis of variation of the optical and infrared fluxes, colour indices, and emission line fluxes suggests that the photometric decline in 2005-2009 resulted from an interplay between variable accretion and circumstellar extinction: since the central luminosity of the system is dominated by accretion, a modest drop in the accretion rate could induce the drastic restructuring of the inner disc. Dust condensation in the inner disc region might have resulted in the enhancement of the circumstellar extinction.Comment: 11 pages, 4 figures, accepted for publication by MNRAS. 3 online tables adde

Cross section of α\alpha-induced reactions on 197^{197}Au at sub-Coulomb energies

Statistical model calculations have to be used for the determination of reaction rates in large-scale reaction networks for heavy-element nucleosynthesis. A basic ingredient of such a calculation is the a-nucleus optical model potential. Several different parameter sets are available in literature, but their predictions of a-induced reaction rates vary widely, sometimes even exceeding one order of magnitude. This paper presents the result of a-induced reaction cross-section measurements on gold which could be carried out for the first time very close to the astrophysically relevant energy region. The new experimental data are used to test statistical model predictions and to constrain the a-nucleus optical model potential. For the measurements the activation technique was used. The cross section of the (a,n) and (a,2n) reactions was determined from g-ray counting, while that of the radiative capture was determined via X-ray counting. The cross section of the reactions was measured below E$_a=20.0$~MeV. In the case of the $^{197}$Au(a,2n)$^{199}$Tl reaction down to 17.5~MeV with 0.5-MeV steps, reaching closer to the reaction threshold than ever before. The cross section of $^{197}$Au(a,n)$^{200}$Tl and $^{197}$Au(a,g)$^{201}$Tl was measured down to E$_a=13.6$ and 14.0~MeV, respectively, with 0.5-MeV steps above the (a,2n) reaction threshold and with 1.0-MeV steps below that. The new dataset is in agreement with the available values from the literature, but is more precise and extends towards lower energies. Two orders of magnitude lower cross sections were successfully measured than in previous experiments which used g-ray counting only, thus providing experimental data at lower energies than ever before. The new precision dataset allows us to find the best-fit a-nucleus optical model potential and to predict cross sections in the Gamow window with smaller uncertainties.Comment: Accepted for publication in Phys. Rev.

Primary alterations during the development of hidradenitis suppurativa

BACKGROUND: Hidradenitis suppurativa (HS) is a chronic, inflammatory disease of the apocrine gland‐rich (AGR) skin region. The initial steps of disease development are not fully understood, despite intense investigations into immune alterations in lesional HS skin. OBJECTIVES: We aimed to systematically investigate the inflammatory molecules involved in three stages of HS pathogenesis, including healthy AGR, non‐lesional HS and lesional HS skin, with the parallel application of multiple mRNA and protein‐based methods. METHODS: Immune cell counts (T cells, dendritic cells, macrophages), Th1/Th17‐related molecules (IL‐12B, TBX21, IFNG, TNFA, IL‐17, IL10, IL‐23A, TGFB1, RORC, CCL20), keratinocyte‐related sensors (TLR2,4), mediators (S100A7, S100A8, S100A9, DEFB4B, LCN2, CAMP, CCL2) and pro‐inflammatory molecules (IL1B, IL6, TNFA, IL‐23A) were investigated in the three groups by RNASeq, RT‐qPCR, immunohistochemistry and immunofluorescence. RESULTS: Epidermal changes were already detectable in non‐lesional HS skin; the epidermal occurrence of antimicrobial peptides (AMPs), IL‐1β, TNF‐α and IL‐23 was highly upregulated compared with healthy AGR skin. In lesional HS epidermis, TNF‐α and IL‐1β expression remained at high levels while AMPs and IL‐23 increased even more compared with non‐lesional skin. In the dermis of non‐lesional HS skin, signs of inflammation were barely detectable (vs. AGR), while in the lesional dermis, the number of inflammatory cells and Th1/Th17‐related mediators were significantly elevated. CONCLUSIONS: Our findings that non‐lesional HS epidermal keratinocytes produce not only AMPs and IL‐1β but also high levels of TNF‐α and IL‐23 confirm the driver role of keratinocytes in HS pathogenesis and highlight the possible role of keratinocytes in the transformation of non‐inflammatory Th17 cells (of healthy AGR skin) into inflammatory cells (of HS) via the production of these mediators. The fact that epidermal TNF‐α and IL‐23 appear also in non‐lesional HS seems to prove these cytokines as excellent therapeutic targets

Chlorine Dioxide Is a Size-Selective Antimicrobial Agent

Background / Aims: ClO2, the so-called "ideal biocide", could also be applied as an antiseptic if it was understood why the solution killing microbes rapidly does not cause any harm to humans or to animals. Our aim was to find the source of that selectivity by studying its reaction-diffusion mechanism both theoretically and experimentally. Methods: ClO2 permeation measurements through protein membranes were performed and the time delay of ClO2 transport due to reaction and diffusion was determined. To calculate ClO2 penetration depths and estimate bacterial killing times, approximate solutions of the reaction-diffusion equation were derived. In these calculations evaporation rates of ClO2 were also measured and taken into account. Results: The rate law of the reaction-diffusion model predicts that the killing time is proportional to the square of the characteristic size (e. g. diameter) of a body, thus, small ones will be killed extremely fast. For example, the killing time for a bacterium is on the order of milliseconds in a 300 ppm ClO2 solution. Thus, a few minutes of contact time (limited by the volatility of ClO2) is quite enough to kill all bacteria, but short enough to keep ClO2 penetration into the living tissues of a greater organism safely below 0.1 mm, minimizing cytotoxic effects when applying it as an antiseptic. Additional properties of ClO2, advantageous for an antiseptic, are also discussed. Most importantly, that bacteria are not able to develop resistance against ClO2 as it reacts with biological thiols which play a vital role in all living organisms. Conclusion: Selectivity of ClO2 between humans and bacteria is based not on their different biochemistry, but on their different size. We hope initiating clinical applications of this promising local antiseptic