Application of Scanning Electron Microscopy and X-Ray Analysis to Urinary Tract Cancer in Animals and Humans

Scanning electron microscopy (SEM) has proven useful in various aspects of urinary bladder carcinogenesis research and these are reviewed as they pertain to our research involving sodium saccharin in the rat. Sodium saccharin-carcinogenesis in rats requires administration at high doses beginning at birth or earlier. Administration beginning at ages of 5 weeks or later results in much lower incidences of bladder tumors. Methods were developed for examining the rat fetal and neonatal bladder to further evaluate effects at these critical ages. Several significant differences were found by SEM between the fetal bladder compared to the-adult. The typical polygonal superficial cells of the bladder with asymmetric unit membrane were present before birth, but the slow turnover rate of the adult bladder did not occur until 3-4 weeks of age. Sodium saccharin causes increased proliferation rates and hyperplasia of the urothelium which is dose-dependent. SEM was found to be more sensitive than either light microscopy or labeling indices to detect the earliest lesions induced by sodium saccharin. More recently, amorphous and crystalline material in the urine of rats fed high doses of sodium saccharin were detected by SEM examinations which contained silicon as well as calcium, phosphate, and magnesium as detected by energy dispersive X-ray analysis (EDS) with the SEM. These parameters may be relevant to differences between rats and humans and pertain to extrapolations regarding risk assessment

English Broadcast News Speech Recognition by Humans and Machines

With recent advances in deep learning, considerable attention has been given to achieving automatic speech recognition performance close to human performance on tasks like conversational telephone speech (CTS) recognition. In this paper we evaluate the usefulness of these proposed techniques on broadcast news (BN), a similar challenging task. We also perform a set of recognition measurements to understand how close the achieved automatic speech recognition results are to human performance on this task. On two publicly available BN test sets, DEV04F and RT04, our speech recognition system using LSTM and residual network based acoustic models with a combination of n-gram and neural network language models performs at 6.5% and 5.9% word error rate. By achieving new performance milestones on these test sets, our experiments show that techniques developed on other related tasks, like CTS, can be transferred to achieve similar performance. In contrast, the best measured human recognition performance on these test sets is much lower, at 3.6% and 2.8% respectively, indicating that there is still room for new techniques and improvements in this space, to reach human performance levels.Comment: \copyright 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other work

Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions.

BackgroundCTCF and BORIS (CTCFL), two paralogous mammalian proteins sharing nearly identical DNA binding domains, are thought to function in a mutually exclusive manner in DNA binding and transcriptional regulation.ResultsHere we show that these two proteins co-occupy a specific subset of regulatory elements consisting of clustered CTCF binding motifs (termed 2xCTSes). BORIS occupancy at 2xCTSes is largely invariant in BORIS-positive cancer cells, with the genomic pattern recapitulating the germline-specific BORIS binding to chromatin. In contrast to the single-motif CTCF target sites (1xCTSes), the 2xCTS elements are preferentially found at active promoters and enhancers, both in cancer and germ cells. 2xCTSes are also enriched in genomic regions that escape histone to protamine replacement in human and mouse sperm. Depletion of the BORIS gene leads to altered transcription of a large number of genes and the differentiation of K562 cells, while the ectopic expression of this CTCF paralog leads to specific changes in transcription in MCF7 cells.ConclusionsWe discover two functionally and structurally different classes of CTCF binding regions, 2xCTSes and 1xCTSes, revealed by their predisposition to bind BORIS. We propose that 2xCTSes play key roles in the transcriptional program of cancer and germ cells

Advanced burning stages and fate of 8-10 Mo stars

The stellar mass range 8<M/Mo<12 corresponds to the most massive AGB stars and the most numerous massive stars. It is host to a variety of supernova progenitors and is therefore very important for galactic chemical evolution and stellar population studies. In this paper, we study the transition from super-AGB star to massive star and find that a propagating neon-oxygen burning shell is common to both the most massive electron capture supernova (EC-SN) progenitors and the lowest mass iron-core collapse supernova (FeCCSN) progenitors. Of the models that ignite neon burning off-center, the 9.5Mo model would evolve to an FeCCSN after the neon-burning shell propagates to the center, as in previous studies. The neon-burning shell in the 8.8Mo model, however, fails to reach the center as the URCA process and an extended (0.6 Mo) region of low Ye (0.48) in the outer part of the core begin to dominate the late evolution; the model evolves to an EC-SN. This is the first study to follow the most massive EC-SN progenitors to collapse, representing an evolutionary path to EC-SN in addition to that from SAGB stars undergoing thermal pulses. We also present models of an 8.75Mo super-AGB star through its entire thermal pulse phase until electron captures on 20Ne begin at its center and of a 12Mo star up to the iron core collapse. We discuss key uncertainties and how the different pathways to collapse affect the pre-supernova structure. Finally, we compare our results to the observed neutron star mass distribution.Comment: 20 pages, 14 figures, 1 table. Submitted to ApJ 2013 February 19; accepted 2013 June

Large outdoor fires and the built environment: summary of kick-off workshop

Presentacions del Workshop celebrat com una part del 11th Asia-Oceania Symposium on Fire Science and Technology (AOSFST) a Taipei, Taiwan.The kickoff workshop of the new permanent working group, sponsored by the International Association for Fire Safety Science (IAFSS), entitled Large Outdoor Fires and the Built Environment was held from 3:00 pm to 4:30 pm on Sunday October 21, 2018. The workshop was held as a part of the 11th Asia-Oceania Symposium on Fire Science and Technology (AOSFST) in Taipei, Taiwan. The working group is co-led by Sara McAllister of the U.S. Forest Service (unable to come to Taiwan), Sayaka Suzuki of National Research Institute of Fire and Disaster, and Samuel L. Manzello of NIST’s Engineering Laboratory. The IAFSS permanent working group consists of three subgroups, with subleaders appointed by Manzello, McAllister, and Suzuki, and these are prioritized into the following topics: Ignition Resistant Communities (IRC – led by Elsa Pastor, UPC, unable to come to Taiwan), Emergency Management and Evacuation (EME, led by Enrico Ronchi, Lund University, unable to come to Taiwan), and Large Outdoor Firefighting (LOFF, led by Raphaele Blanchi, CSIRO). The IRC subgroup is focused on developing the scientific basis for new standard testing methodologies indicative of large outdoor fire exposures, including the development of necessary testing methodologies to characterize wildland fuel treatments adjacent to communities. The EME subgroup is focused on developing the scientific basis for effective emergency management strategies for communities exposed to large outdoor fires. The LOFF subgroup is providing a review of various tactics that are used, as well as the various personal protective equipment (PPE), and suggest pathways for research community engagement, including environmental issues in suppressing these fires. The overall objectives are to bring the full depth of knowledge of the IAFSS community to work on these priority topics. At the kickoff workshop, detailed ideas were presented regarding the planned activities of the working group, especially the large workshop to be held at IAFSS 2020.Peer ReviewedPostprint (published version

Towards understanding structure of the monopole clusters

We consider geometrical characteristics of monopole clusters of the lattice SU(2) gluodynamics. We argue that the polymer approach to the field theory is an adequate means to describe the monopole clusters. Both finite-size and the infinite, or percolating clusters are considered. We find out that the percolation theory allows to reproduce the observed distribution of the finite-size clusters in their length and radius. Geometrical characteristics of the percolating cluster reflect, in turn, the basic properties of the ground state of a system with a gap.Comment: 20 pages, RevTeX

On the Landau Ginzburg theory of MAG projected SU(2) lattice gauge theory

Maximal Abelian gauge fixing and subsequent Abelian projection of SU(2) lattice gauge theory defines closed trajectories of magnetic monopoles. These trajectories can be interpreted in terms of an effective scalar field theory of the MAG monopoles using the worldline representation of the functional determinants. Employing the monopole worldlines detected in the numerical simulation, we show that a scalar bound state exists. The screening mass $m$ of this state properly scales towards the continuum limit. We find m ~ 1.3 $GeV when the string tension sigma = 440 MeV is used as reference scale.Comment: 9 pages, 3 figures, accepted by Phys. Lett.

Risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of aedes aegypti (linnaeus) in some sites in Accra, Ghana

Background: Dengue is one of the emerging diseases that can mostly only be controlled by vector control since there is no vaccine for the disease. Although, Dengue has not been reported in Ghana, movement of people from neighbouring countries where the disease has been reported can facilitate transmission of the disease. Objective: This study was carried on the University of Ghana campus to determine the risk of transmission of viral haemorrhagic fevers and the insecticide susceptibility status of Ae. aegypti in some sites in Accra, Ghana. Design: Larval surveys were carried to inspect containers within households and estimate larval indices and adult Aedes mosquitoes were collected using human landing collection technique. WHO tube assays was used to assess the insecticide susceptibility status of Aedes mosquitoes. Results: Ae. aegypti were the most prevalent species, 75.5% and followed by Ae. vittatus, 23.9 %. Ae. albopictus and Ae. granti were in smaller numbers. Household index (HI), Breteau index (BI), and container index were calculated as 8.2%, 11.2% and 10.3% respectively with man-vector contact rate of 0.67 bites/man-hour estimated for the area. The mortalities recorded for Ae. aegypti from WHO tube assays was 88%, 94%, 80% and 99% for DDT (4%), deltamethrin (0.05%), lambdacyhalothrin (0.05%) and permethrin (0.75%) respectively.Conclusion: The survey results indicated that the density of Aedes mosquitoes was considered to be sufficient to promote an outbreak of viral haemorrhagic fevers on Legon Campus. Aedes mosquitoes were found to be resistant to DDT, deltamethrin and lamdacyhalothrin, but susceptible to permethrin. Funding: This study was supported in part by Japan Initiative for Global Research Network on Infectious Diseases(J-Grid).Keywords: Mosquitoes, Aedes aegypti, insecticide, risk, VHF transmission, Ghan

Differential Geometry of Group Lattices

In a series of publications we developed "differential geometry" on discrete sets based on concepts of noncommutative geometry. In particular, it turned out that first order differential calculi (over the algebra of functions) on a discrete set are in bijective correspondence with digraph structures where the vertices are given by the elements of the set. A particular class of digraphs are Cayley graphs, also known as group lattices. They are determined by a discrete group G and a finite subset S. There is a distinguished subclass of "bicovariant" Cayley graphs with the property that ad(S)S is contained in S. We explore the properties of differential calculi which arise from Cayley graphs via the above correspondence. The first order calculi extend to higher orders and then allow to introduce further differential geometric structures. Furthermore, we explore the properties of "discrete" vector fields which describe deterministic flows on group lattices. A Lie derivative with respect to a discrete vector field and an inner product with forms is defined. The Lie-Cartan identity then holds on all forms for a certain subclass of discrete vector fields. We develop elements of gauge theory and construct an analogue of the lattice gauge theory (Yang-Mills) action on an arbitrary group lattice. Also linear connections are considered and a simple geometric interpretation of the torsion is established. By taking a quotient with respect to some subgroup of the discrete group, generalized differential calculi associated with so-called Schreier diagrams are obtained.Comment: 51 pages, 11 figure