Quaking Aspen Ecology on Forest Service Lands North of Yellowstone National Park

Quaking aspen (Populus tremuloides) occupy a small area in the northern Rocky Mountains, but are highly valued as wildlife habitat. Aspen stands in and around Yellowstone National Park commonly consist of few, large, mature overstory stems and numerous root suckers that do not grow above the browsing reach (≈ 2 m) of most wild ungulates. Our primary objective was to determine if the recruitment or density of aspen stems \u3e 2 m tall had changed from 1991 to 2006 on a portion of the Gallatin National Forest. The same aspen stands were surveyed in 1991 and 2006 in the 560 km² study area (n = 316). Secondary objectives were to determine if aspen density was influenced by elk (Cervus elaphus) browsing, conifer establishment, and cattle (Bos spp.) grazing. Mean recruitment stem density did not change from 1991 to 2006 (P = 0.95). Density of stems \u3e 2 m declined 12 percent from 1991 to 2006 (P = 0.04), which indicates that recruitment stems are not being produced at a sufficient rate to replace aging overstories. Areas with the greatest elk densities had the lowest recruitment stem densities and contributed the most to the decline. Although elk browsing seemed to play the largest role, conifer establishment and cattle grazing have also negatively impacted overstory recruitment in aspen stands. Even though elk numbers on the Northern Yellowstone Winter Range have declined since wolf reintroduction, aspen recruitment has not increased at the landscape level on the Gallatin National Forest

Performance Metrics and Empirical Results of a PUF Cryptographic Key Generation ASIC

We describe a PUF design with integrated error correction that is robust to various layout implementations and achieves excellent and consistent results in each of the following four areas: Randomness, Uniqueness, Bias and Stability. 133 PUF devices in 0.13 μm technology encompassing seven circuit layout implementations were tested. The PUF-based key generation design achieved less than 0.58 ppm failure rates with 50%+ stability safety margin. 1.75M error correction blocks ran error-free under worst-case V/T corners (±10% V, 125°C/-65°C) and under voltage extremes of ±20% V. All PUF devices demonstrated excellent NIST-random behavior (99 cumulative percentile), a criterion used to qualify random sources for use as keying material for cryptographic-grade applications

Development and Initial Findings of an Implementation Process Measure for Child Welfare System Change

This article describes a new measure designed to examine the process of implementation of child welfare systems change. The measure was developed to document the status of the interventions and strategies that are being implemented and the drivers that are being installed to achieve sustainable changes in systems. The measure was used in a Children’s Bureau-supported national effort to assess the ongoing implementation of 24 systems-change projects in child welfare jurisdictions across the country. The article describes the process for measure development, method of administration and data collection, and quantitative and qualitative findings

Diagnostic Potential of the NMDA Receptor Peptide Assay for Acute Ischemic Stroke

Background The acute assessment of patients with suspected ischemic stroke remains challenging. The use of brain biomarker assays may improve the early diagnosis of ischemic stroke. The main goal of the study was to evaluate whether the NR2 peptide, a product of the proteolytic degradation of N-methyl-D-aspartate (NMDA) receptors, can differentiate acute ischemic stroke (IS) from stroke mimics and persons with vascular risk factors/healthy controls. A possible correlation between biomarker values and lesion sizes was investigated as the secondary objective. Methods and Findings A total of 192 patients with suspected stroke who presented within 72 h of symptom onset were prospectively enrolled. The final diagnosis was determined based on clinical observations and radiological findings. Additionally gender- and age-matched healthy controls (n = 52) and persons with controlled vascular risk factors (n = 48) were recruited to compare NR2 peptide levels. Blinded plasma was assayed by rapid magnetic particles (MP) ELISA for NR2 peptide within 30 min and results for different groups compared using univariate and multivariate statistical analyses. There was a clinical diagnosis of IS in 101 of 192 (53%) and non-stroke in 91 (47%) subjects. The non-stroke group included presented with acute stroke symptoms who had no stroke (n = 71) and stroke mimics (n = 20). The highest NR2 peptide elevations where found in patients with IS that peaked at 12 h following symptom onset. When the biomarker cut off was set at 1.0 ug/L, this resulted in a sensitivity of 92% and a specificity of 96% to detect IS. A moderate correlation (rs = 0.73) between NR2 peptide values and acute ischemic cortical lesions (\u3c200 \u3emL) was found. Conclusions This study suggests that the NR2 peptide may be a brain specific biomarker to diagnose acute IS and may allow the differentiation of IS from stroke mimics and controls. Additional larger scale clinical validation studies are required

Hands-on Computer Use in Science Classrooms: The Skeptics Are Still Waiting

Frank Collea was a friend of Robert Fuller and David Brooks, and a mentor to Brooks. We miss him for his energy, his enthusiasm for teaching science, and his perception about how to improve science education. Frank Collea was not a big fan of using computers in instruction. Frank was neither an advocate of using computers to deliver instruction, nor an advocate of teaching their use as professional tools. Indeed, he thought that most of those of us who advocate computer use make assertions that are unwarranted. A decade ago, desktop computers were beginning to appear in colleges and universities in small numbers, and we began to explore their use (Sowell and Fuller, 1990). Since then, our thinking has changed substantially, moving away from having computers serve as patient teachers of the classical curriculum, and toward using them as professional tools—to extend, to magnify, to expand, and to enhance human reasoning. This article deals with the issues related to students learning to use computers as such professional tools. Two qualitative data sources inform this paper. The first is a recent doctoral dissertation consisting of a case study of a ‘mathematical methods in physics’ course that incorporated the use of Maple™* software (Runge, 1997). The other is an evaluation of a new undergraduate course, ‘multimedia physics,’ that sought to integrate mathematics and physics content, and involved the use of many media forms (Pytlik Z. and Spiegel, 1997)

Molecular Mechanisms of the Diabetogenic Effects of Arsenic: Inhibition of Insulin Signaling by Arsenite and Methylarsonous Acid

BACKGROUND: Increased prevalences of diabetes mellitus have been reported among individuals chronically exposed to inorganic arsenic (iAs). However, the mechanisms underlying the diabetogenic effects of iAs have not been characterized. We have previously shown that trivalent metabolites of iAs, arsenite (iAs(III)) and methylarsonous acid (MAs(III)) inhibit insulin-stimulated glucose uptake (ISGU) in 3T3-L1 adipocytes by suppressing the insulin-dependent phosphorylation of protein kinase B (PKB/Akt). OBJECTIVES: Our goal was to identify the molecular mechanisms responsible for the suppression of PKB/Akt phosphorylation by iAs(III) and MAs(III). METHODS: The effects of iAs(III) and MAs(III) on components of the insulin-activated signal transduction pathway that regulate PKB/Akt phosphorylation were examined in 3T3-L1 adipocytes. RESULTS: Subtoxic concentrations of iAs(III) or MAs(III) had little or no effect on the activity of phosphatidylinositol 3-kinase (PI-3K), which synthesizes phosphatidylinositol-3,4,5-triphosphate (PIP(3)), or on phosphorylation of PTEN (phosphatase and tensin homolog deleted on chromosome ten), a PIP(3) phosphatase. Neither iAs(III) nor MAs(III) interfered with the phosphorylation of 3-phosphoinositide-dependent kinase-1 (PDK-1) located downstream from PI-3K. However, PDK-1 activity was inhibited by both iAs(III) and MAs(III). Consistent with these findings, PDK-1-catalyzed phosphorylation of PKB/Akt(Thr308) and PKB/Akt activity were suppressed in exposed cells. In addition, PKB/Akt(Ser473) phosphorylation, which is catalyzed by a putative PDK-2, was also suppressed. Notably, expression of constitutively active PKB/Akt restored the normal ISGU pattern in adipocytes treated with either iAs(III) or MAs(III). CONCLUSIONS: These results suggest that inhibition of the PDK-1/PKB/Akt-mediated transduction step is the key mechanism for the inhibition of ISGU in adipocytes exposed to iAs(III) or MAs(III), and possibly for impaired glucose tolerance associated with human exposures to iAs

Examining the Proteome of Drosophila Across Organism Lifespan

A survey of the proteome of Drosophila melanogaster at nine time points across the adult lifespan based on several mass-spectrometry-based techniques is presented. In total, there is evidence for 5902 unique peptides corresponding to 1699 different proteins. Of hundreds of relatively abundant components, many appear to be highly dynamic as the adult fly ages. Of those proteins that we observe changing with age, a majority, associated with metabolism, reproduction, and development, are downregulated. Other biological pathways such as defense response also show variable changes, where some proteins are down-regulated and others are up-regulated. The observed variations are compared with a report of genome-wide changes at the transcriptome level at different ages and the similarities and differences are presented

BACE1 activity impairs neuronal glucose oxidation:rescue by beta-hydroxybutyrate and lipoic acid

Glucose hypometabolism and impaired mitochondrial function in neurons have been suggested to play early and perhaps causative roles in Alzheimer's disease (AD) pathogenesis. Activity of the aspartic acid protease, beta-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1), responsible for beta amyloid peptide generation, has recently been demonstrated to modify glucose metabolism. We therefore examined, using a human neuroblastoma (SH-SY5Y) cell line, whether increased BACE1 activity is responsible for a reduction in cellular glucose metabolism. Overexpression of active BACE1, but not a protease-dead mutant BACE1, protein in SH-SY5Y cells reduced glucose oxidation and the basal oxygen consumption rate, which was associated with a compensatory increase in glycolysis. Increased BACE1 activity had no effect on the mitochondrial electron transfer process but was found to diminish substrate delivery to the mitochondria by inhibition of key mitochondrial decarboxylation reaction enzymes. This BACE1 activity-dependent deficit in glucose oxidation was alleviated by the presence of beta hydroxybutyrate or α-lipoic acid. Consequently our data indicate that raised cellular BACE1 activity drives reduced glucose oxidation in a human neuronal cell line through impairments in the activity of specific tricarboxylic acid cycle enzymes. Because this bioenergetic deficit is recoverable by neutraceutical compounds we suggest that such agents, perhaps in conjunction with BACE1 inhibitors, may be an effective therapeutic strategy in the early-stage management or treatment of AD

Multimodal imaging of the self-regulating developing brain

Self-regulation refers to the ability to control behavior, cognition, and emotions, and self-regulation failure is related to a range of neuropsychiatric problems. It is poorly understood how structural maturation of the brain brings about the gradual improvement in self-regulation during childhood. In a large-scale multicenter effort, 735 children (4-21 y) underwent structural MRI for quantification of cortical thickness and surface area and diffusion tensor imaging for quantification of the quality of major fiber connections. Brain development was related to a standardized measure of cognitive control (the flanker task from the National Institutes of Health Toolbox), a critical component of self-regulation. Ability to inhibit responses and impose cognitive control increased rapidly during preteen years. Surface area of the anterior cingulate cortex accounted for a significant proportion of the variance in cognitive performance. This finding is intriguing, because characteristics of the anterior cingulum are shown to be related to impulse, attention, and executive problems in neurodevelopmental disorders, indicating a neural foundation for self-regulation abilities along a continuum from normality to pathology. The relationship was strongest in the younger children. Properties of large-fiber connections added to the picture by explaining additional variance in cognitive control. Although cognitive control was related to surface area of the anterior cingulate independently of basic processes of mental speed, the relationship between white matter quality and cognitive control could be fully accounted for by speed. The results underscore the need for integration of different aspects of brain maturation to understand the foundations of cognitive development