A study of the relationship between cloud-to-ground lightning and precipitation in the convective weather system in China

International audienceIn this paper, the correlation between cloud-to-ground (CG) lightning and precipitation has been studied by making use of the data from weather radar, meteorological soundings, and a lightning location system that includes three direction finders about 40 km apart from each other in the Pingliang area of east Gansu province in P. R. China. We have studied the convective systems that developed during two cold front processes passing over the observation area, and found that the CG lightning can be an important factor in the precipitation estimation. The regression equation between the average precipitation intensity (R) and the number of CG lightning flashes (L) in the main precipitation period is R = 1.69 ln (L) - 0.27, and the correlation coefficient r is 0.86. The CG lightning flash rate can be used as an indicator of the formation and development of the convective weather system. Another more exhaustive precipitation estimation method has been developed by analyzing the temporal and spatial distributions of the precipitation relative to the location of the CG lightning flashes. Precipitation calculated from the CG lightning flashes is very useful, especially in regions with inadequate radar cover

Symbiotic performance of Medicago ciliaris lines in association with different Sinorhizobium strains

We analyzed nodulation and nitrogen fixation efficiency for seven lines of Medicago ciliaris in association with two references strains of Sinorhizobium medicae (M104) and S. meliloti (RCR2011). These M. ciliaris lines were collected in Northern-East (Enfidha and Soliman) and Western (Rhayet and Mateur) Tunisian areas. The M. truncatula reference line Jemalong A17 (JA17) was also included. Plants were harvested after a culture period of 60 days. Two quantitative traits were measured at the harvest including the mean number of nodules per plant and the aerial dry weight (ADW). Analysis of variance showed that nodulation and nitrogen fixation efficiency (NFE) were dependent on the effects of line, strain and their interaction. The highest levels were observed for strain (45.07%) and line (53.24%) effects, respectively, for nodulation and NFE. While studied lines showed generally more number of nodules with RCR2011, they were more efficient with M104 strain. No significant difference in number of nodules was detected between M. ciliaris lines and JA17 with M104, whereas RCR2011 was generally most infective with JA17. Overall, JA17 line exhibited the largest NFE with both strains. Furthermore, there was no significant (P>0.05) correlation between nodulation and NFE for M. ciliaris line by strain associations

Design of double-walled carbon nanotubes for biomedical applications

Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT–siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). Uptake of the custom designed nanotubes was not associated with detectable biochemical perturbations in cultured cells, but transfection of cells with DWNTs loaded with siRNA targeting the green fluorescent protein (GFP) gene, serving as a model system, as well as with therapeutic siRNA targeting the survivin gene, led to a significant gene silencing effect, and in the latter case a resulting apoptotic effect in cancer cells

Building Information Modelling (BIM) value realisation framework for asset owners

The paper is presenting a value realisation framework for asset owners based on an exploratory study. The study is descriptive in nature and adopting a qualitative approach towards data collection. The paper adopts the viewpoint of BIM business value measurement considering that; (i) if the process is better as a result of BIM-based processes, then it is different in some relevant way; (ii) if it is different in some relevant way as a result of certain BIM properties or characteristics, then the change is observable; (iii) if the change is observ-able because of certain direct BIM benefits, then it is countable; (iv) if it is countable using defined measurement metrics, then it is measurable; (v) if it is measurable using established measurement techniques, an organisation can value each unit and therefore, realise the benefits of BIM. The specific contribution of paper is to improve asset owners’ understanding of BIM-business value measurement techniques and approaches

Multi-instrumental analysis of large sprite events and their producing storm in southern France

During the night of 01-02 September, 2009, seventeen distinct sprite events including 3 halos were observed above a storm in north-western Mediterranean Sea, with a video camera at Pic du Midi (42.93N; 0.14E; 2877m). The sprites occurred at distances between 280 and 390km which are estimated based on their parent CG location. The MCS-type storm was characterized by a trailing-stratiform structure and a very circular shape with a size of about 70,000km2 (cloud top temperature lower than -35°C) when the TLEs were observed. The cloud to ground (CG) flash rate was large (45min-1) one hour before the TLE observation and very low (<5min-1) during it. Out of the 17 sprite events, 15 parent+CG (P+CG) strokes have been identified and their average peak current is 87kA (67kA for the 14 events without halo), while the associated charge moment changes (CMC) that could be determined, range from 424 to 2088±20%Ckm. Several 2-second videos contain multiple sprite events: one with four events, one with three events and three with two events. Column and carrot type sprites are identified, either together or separately. All P+CG strokes are clearly located within the stratiform region of the storm and the second P+CG stroke of a multiple event is back within the stratiform region. Groups of large and bright carrots reach ~70km height and ~80km horizontal extent. These groups are associated with a second pulse of electric field radiation in the ELF range which occurs ~5ms after the P+CG stroke and exhibits the same polarity, which is evidence for current in the sprite body. VLF perturbations associated with the sprite events were recorded with a station in Algiers. © 2012 Elsevier B.V

Reliability and psychometric properties of the Greek translation of the State-Trait Anxiety Inventory form Y: Preliminary data

BACKGROUND: The State-Trait Anxiety Inventory form Y is a brief self-rating scale for the assessment of state and trait anxiety. The aim of the current preliminary study was to assess the psychometric properties of its Greek translation. MATERIALS AND METHODS: 121 healthy volunteers 27.22 ± 10.61 years old, and 22 depressed patients 29.48 ± 9.28 years old entered the study. In 20 of them the instrument was re-applied 1–2 days later. Translation and Back Translation was made. The clinical diagnosis was reached with the SCAN v.2.0 and the IPDE. The Symptoms Rating Scale for Depression and Anxiety (SRSDA) and the EPQ were applied for cross-validation purposes. The Statistical Analysis included the Pearson Correlation Coefficient and the calculation of Cronbach's alpha. RESULTS: The State score for healthy subjects was 34.30 ± 10.79 and the Trait score was 36.07 ± 10.47. The respected scores for the depressed patients were 56.22 ± 8.86 and 53.83 ± 10.87. Both State and Trait scores followed the normal distribution in control subjects. Cronbach's alpha was 0.93 for the State and 0.92 for the Trait subscale. The Pearson Correlation Coefficient between State and Trait subscales was 0.79. Both subscales correlated fairly with the anxiety subscale of the SRSDA. Test-retest reliability was excellent, with Pearson coefficient being between 0.75 and 0.98 for individual items and equal to 0.96 for State and 0.98 for Trait. CONCLUSION: The current study provided preliminary evidence concerning the reliability and the validity of the Greek translation of the STAI-form Y. Its properties are generally similar to those reported in the international literature, but further research is necessary

Observations of the relationship between sprite morphology and in-cloud lightning processes

[1] During a thunderstorm on 23 July 2003, 15 sprites were captured by a LLTV camera mounted at the observatory on Pic du Midi in the French Pyrénées. Simultaneous observations of cloud-to-ground (CG) and intracloud (IC) lightning activity from two independent lightning detection systems and a broadband ELF/VLF receiver allow a detailed study of the relationship between electrical activity in a thunderstorm and the sprites generated in the mesosphere above. Results suggest that positive CG and IC lightning differ for the two types of sprites most frequently observed, the carrot- and column-shaped sprites. Column sprites occur after a short delay (<30 ms) from the causative +CG and are associated with little VHF activity, suggesting no direct IC action on the charge transfer process. On the other hand, carrot sprites are delayed up to about 200 ms relative to their causative +CG stroke and are accompanied by a burst of VHF activity starting 25–75 ms before the CG stroke. While column sprites associate with short-lasting (less than 30 ms) ELF/VLF sferics, carrot sprites associate with bursts of sferics initiating at the time of the causative +CG discharge and persisting for 50 to 250 ms, indicating extensive in-cloud activity. One carrot event was found to be preceded by vigorous IC activity and a strong, long-lived cluster of ELF/VLF sferics but lacking a +CG. The observations of ELF/VLF sferic clusters associated with lightning and sprites form the basis for a discussion of the reliability of lightning detection systems based on VHF interferometry.Peer ReviewedPostprint (published version

Chloroquine-enhanced gene delivery mediated by carbon nanotubes

Polyethyleneimine-coated double-walled carbon nanotubes (DWCNTs) were used for dual gene and drug delivery, after loading the DWCNTs with the drug chloroquine, a lysosomotropic compound that is able to promote escape from the lysosomal compartment. Different forms of functionalization of the DWCNTs were examined in order to optimize this system. They included the testing of different treatments on DWCNTs to optimize the loading and delivery of chloroquine and the selection of a cationic polymer for coating the DWCNTs for optimum DNA binding and delivery. An acid oxidation treatment of DWCNTs was selected for optimum chloroquine loading together with polyethyleneimine as optimum cationic coating agent for plasmid DNA binding. Optimization of the conditions for choroquine-enhanced gene delivery were developed using luciferase expression as a model system. We have demonstrated that chloroquine-loading increases the ability of polyethyleneimine-coated DWCNTs to deliver functional nucleic acid to human cells. Cell viability tests have shown no cytotoxicity of the functionalized DWCNTs at the concentrations needed for optimum gene delivery. These results support the potential applications of this methodology in gene therapy