134 research outputs found
Diseño del sistema de agua potable para mejorar las condiciones de vida de la población de la localidad de Cuchulia, distrito Jazán, provincia Bongará, región Amazonas para el año 2015
El presente trabajo de investigación consistió en la propuesta de un diseño del sistema de agua potable por gravedad con planta de tratamiento que abastezca agua de la quebrada Anshe, con la calidad y cantidad adecuada a los pobladores de la localidad de Cuchulia, distrito de Jazán, provincia de Bongará, región de Amazonas. Previamente se aplicó como instrumento una encuesta a 31 pobladores, detectándose la insatisfacción por la falta de este servicio. Los datos procesados permitieron constatar la situación problemática y para ello se formuló la propuesta del diseño, tomando como base la estadística poblacional y proyección de tasa de crecimiento proporcionada por INEI. Los diseños propuestos consideraron los caudales, la población beneficiaria, el levantamiento topográfico, estudios de laboratorio, cálculos hidráulicos y cálculos estructurales. Finalmente, se concluyó que el diseño propuesto debería ejecutarse porque mejoraría el abastecimiento de agua en calidad y cantidad para la población de Cuchulia
Homocisteína en mujeres menopáusicas de Lima
Abstract Objectives: To compare serum homocysteine levels according to menopausal status in a group of women. Design: Descriptive study. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Women 40 to 60 year-old. Interventions: The menopausal status was determined clinically and by serum FSH and estradiol in 97 women 40 to 60 year-old attended at San Bartolome Hospital, Lima, Peru. Plasma homocysteine was determined by fluorescence polarization immunoanalysis. Main outcome measures: Homocysteine levels. Results: Homocysteine levels were within international referential values (5-15 μmol/L). Postmenopausal women levels were significantly higher compared to premenopausal women levels (p< 0.05). Conclusions: Homocysteine levels are increased in menopausal women and could be considered in cardiovascular disease prediction.Objetivos: Comparar los niveles de homocisteína según el estado menopáusico en un grupo de mujeres atendidas en un hospital de Lima. Diseño: Estudio descriptivo. Lugar: Centro de Investigación de Bioquímica y Nutrición, Facultad Medicina, Universidad Nacional Mayor San Marcos, Lima, Perú. Participantes: Mujeres de 40 a 60 años de edad. Intervenciones: En 97 mujeres de 40 a 60 años de edad atendidas en el Hospital San Bartolomé, Lima, Perú, se determinó el estado menopáusico en base a evaluación clínica y dosaje de FSH y estradiol en sangre. Para determinar la homocisteína plasmática se usó el método de inmunoanálisis de polarización de fluorescencia. Principales medidas de resultados: Niveles de homocisteína en sangre. Resultados: Los niveles de homocisteína hallados estuvieron dentro de los valores referenciales internacionales (5 a 15 μmol/L). Las mujeres posmenopáusicas presentaron niveles de homocisteína significativamente mayores comparados con las mujeres premenopáusicas (p<0,05). Conclusiones: Los niveles plasmáticos de homocisteína fueron mayores en las mujeres menopáusicas y su incremento podría ser considerado en la predicción de la enfermedad cardiovascular
Corrigendum: Variability of Bacterial Essential Genes Among Closely Related Bacteria: The Case of Escherichia coli
The definition of bacterial essential genes has been widely pursued using different approaches. Their study has impacted several fields of research such as synthetic biology, the construction of bacteria with minimal chromosomes, the search for new antibiotic targets, or the design of strains with biotechnological applications. Bacterial genomes are mosaics that only share a small subset of gene-sequences (core genome) even among members of the same species. It has been reported that the presence of essential genes is highly variable between closely related bacteria and even among members of the same species, due to the phenomenon known as “non-orthologous gene displacement” that refers to the coding for an essential function by genes with no sequence homology due to horizontal gene transfer (HGT). The existence of dormant forms among bacteria and the high incidence of HGT have been proposed to be driving forces of bacterial evolution, and they might have a role in the low level of conservation of essential genes among related bacteria by non-orthologous gene displacement, but this correlation has not been recognized. The aim of this mini-review is to give a brief overview of the approaches that have been taken to define and study essential genes, and the implications of non-orthologous gene displacement in bacterial evolution, focusing mainly in the case of Escherichia coli. To this end, we reviewed the available literature, and we searched for the presence of the essential genes defined by mutagenesis in the genomes of the 63 best-sequenced E. coli genomes that are available in NCBI database. We could not document specific cases of non-orthologous gene displacement among the E. coli strains analyzed, but we found that the quality of the genome-sequences in the database is not enough to make accurate predictions about the conservation of essential-genes among members of this bacterial species
Novel Application of Cyclolipopeptide Amphisin: Feasibility Study as Additive to Remediate Polycyclic Aromatic Hydrocarbon (PAH) Contaminated Sediments
To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73’s growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production
Opuntia in México: Identifying Priority Areas for Conserving Biodiversity in a Multi-Use Landscape
BACKGROUND: México is one of the world's centers of species diversity (richness) for Opuntia cacti. Yet, in spite of their economic and ecological importance, Opuntia species remain poorly studied and protected in México. Many of the species are sparsely but widely distributed across the landscape and are subject to a variety of human uses, so devising implementable conservation plans for them presents formidable difficulties. Multi-criteria analysis can be used to design a spatially coherent conservation area network while permitting sustainable human usage. METHODS AND FINDINGS: Species distribution models were created for 60 Opuntia species using MaxEnt. Targets of representation within conservation area networks were assigned at 100% for the geographically rarest species and 10% for the most common ones. Three different conservation plans were developed to represent the species within these networks using total area, shape, and connectivity as relevant criteria. Multi-criteria analysis and a metaheuristic adaptive tabu search algorithm were used to search for optimal solutions. The plans were built on the existing protected areas of México and prioritized additional areas for management for the persistence of Opuntia species. All plans required around one-third of México's total area to be prioritized for attention for Opuntia conservation, underscoring the implausibility of Opuntia conservation through traditional land reservation. Tabu search turned out to be both computationally tractable and easily implementable for search problems of this kind. CONCLUSIONS: Opuntia conservation in México require the management of large areas of land for multiple uses. The multi-criteria analyses identified priority areas and organized them in large contiguous blocks that can be effectively managed. A high level of connectivity was established among the prioritized areas resulting in the enhancement of possible modes of plant dispersal as well as only a small number of blocks that would be recommended for conservation management
Rhamnolipids: diversity of structures, microbial origins and roles
Rhamnolipids are glycolipidic biosurfactants produced by various bacterial species. They were initially found as exoproducts of the opportunistic pathogen Pseudomonas aeruginosa and described as a mixture of four congeners: α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha-Rha-C10-C10), α-L-rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoate (Rha-Rha-C10), as well as their mono-rhamnolipid congeners Rha-C10-C10 and Rha-C10. The development of more sensitive analytical techniques has lead to the further discovery of a wide diversity of rhamnolipid congeners and homologues (about 60) that are produced at different concentrations by various Pseudomonas species and by bacteria belonging to other families, classes, or even phyla. For example, various Burkholderia species have been shown to produce rhamnolipids that have longer alkyl chains than those produced by P. aeruginosa. In P. aeruginosa, three genes, carried on two distinct operons, code for the enzymes responsible for the final steps of rhamnolipid synthesis: one operon carries the rhlAB genes and the other rhlC. Genes highly similar to rhlA, rhlB, and rhlC have also been found in various Burkholderia species but grouped within one putative operon, and they have been shown to be required for rhamnolipid production as well. The exact physiological function of these secondary metabolites is still unclear. Most identified activities are derived from the surface activity, wetting ability, detergency, and other amphipathic-related properties of these molecules. Indeed, rhamnolipids promote the uptake and biodegradation of poorly soluble substrates, act as immune modulators and virulence factors, have antimicrobial activities, and are involved in surface motility and in bacterial biofilm development
Microbial surfactants: fundamentals and applicability in the formulation of nano-sized drug delivery vectors
Microbial surfactants, so-called biosurfactants, comprise a wide variety of structurally distinct amphipathic molecules produced by several microorganisms. Besides exhibiting surface activity at the interfaces, these molecules present powerful characteristics including high biodegradability, low toxicity and special biological activities (e.g. antimicrobial, antiviral, anticancer, among others), that make them an alternative to their chemical counterparts. Several medical-related applications have been suggested for these molecules, including some reports on their potential use in the formulation of nano-sized drug delivery vectors. However, despite their promises, due to the generalized lack of knowledge on microbial surfactants phase behavior and stability under diverse physicochemical conditions, these applications remain largely unexplored, thus representing an exciting field of research. These nano-sized vectors are a powerful approach towards the current medical challenges regarding the development of efficient and targeted treatments for several diseases. In this review, a special emphasis will be given to nanoparticles and microemulsions. Nanoparticles are very auspicious as their size, shape and stability can be manipulated by changing the environmental conditions. On the other hand, the easiness of formulation, as well as the broad possibilities of administration justifies the recent popularity of the microemulsions. Notwithstanding, both vector types still require further developments to overcome some critical limitations related with toxicity and costs, among others. Such developments may include the search for other system components, as the microbial surfactants, that can display improved features.The author acknowledges the financial support from the Strategic Project PEst-OE/EQB/LA0023/2013 and project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462) funded by Fundacao para a Ciencia e a Tecnologia
High-risk human papillomavirus (HPV) DNA sequences in metaplastic breast carcinomas of Mexican women
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