Conservation and Diversity of Seed Associated Endophytes in Zea across Boundaries of Evolution, Ethnography and Ecology

Endophytes are non-pathogenic microbes living inside plants. We asked whether endophytic species were conserved in the agriculturally important plant genus Zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from Mexico to Canada. Kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and DNA fingerprinting using terminal restriction fragment length polymorphism (TRFLP) of 16S rDNA. Principle component analysis of TRFLP data showed that seed endophyte community composition varied in relation to plant host phylogeny. However, there was a core microbiota of endophytes that was conserved in Zea seeds across boundaries of evolution, ethnography and ecology. The majority of seed endophytes in the wild ancestor persist today in domesticated maize, though ancient selection against the hard fruitcase surrounding seeds may have altered the abundance of endophytes. Four TRFLP signals including two predicted to represent Clostridium and Paenibacillus species were conserved across all Zea genotypes, while culturing showed that Enterobacter, Methylobacteria, Pantoea and Pseudomonas species were widespread, with γ-proteobacteria being the prevalent class. Twenty-six different genera were cultured, and these were evaluated for their ability to stimulate plant growth, grow on nitrogen-free media, solubilize phosphate, sequester iron, secrete RNAse, antagonize pathogens, catabolize the precursor of ethylene, produce auxin and acetoin/butanediol. Of these traits, phosphate solubilization and production of acetoin/butanediol were the most commonly observed. An isolate from the giant Mexican landrace Mixteco, with 100% identity to Burkholderia phytofirmans, significantly promoted shoot potato biomass. GFP tagging and maize stem injection confirmed that several seed endophytes could spread systemically through the plant. One seed isolate, Enterobacter asburiae, was able to exit the root and colonize the rhizosphere. Conservation and diversity in Zea-microbe relationships are discussed in the context of ecology, crop domestication, selection and migration

Study of the doubly charmed tetraquark T+cc

Quantum chromodynamics, the theory of the strong force, describes interactions of coloured quarks and gluons and the formation of hadronic matter. Conventional hadronic matter consists of baryons and mesons made of three quarks and quark-antiquark pairs, respectively. Particles with an alternative quark content are known as exotic states. Here a study is reported of an exotic narrow state in the D0D0π+ mass spectrum just below the D*+D0 mass threshold produced in proton-proton collisions collected with the LHCb detector at the Large Hadron Collider. The state is consistent with the ground isoscalar T+cc tetraquark with a quark content of ccu⎯⎯⎯d⎯⎯⎯ and spin-parity quantum numbers JP = 1+. Study of the DD mass spectra disfavours interpretation of the resonance as the isovector state. The decay structure via intermediate off-shell D*+ mesons is consistent with the observed D0π+ mass distribution. To analyse the mass of the resonance and its coupling to the D*D system, a dedicated model is developed under the assumption of an isoscalar axial-vector T+cc state decaying to the D*D channel. Using this model, resonance parameters including the pole position, scattering length, effective range and compositeness are determined to reveal important information about the nature of the T+cc state. In addition, an unexpected dependence of the production rate on track multiplicity is observed

Lanthanide complexes of 2,6-dihydroxybenzoic acid: synthesis, crystal structures and luminescent properties of [nBu(4)N](2)[Ln(2,6-dhb)(5)(H(2)O)(2)] (Ln = Sm and Tb)

Lanthanide complexes of 2,6-dihydroxybenzoic acid (2,6- Hdhb), namely [nBu4N]2[Ln(2,6-dhb)5(H2O)2] [Ln = Sm (1) and Tb (2)], were synthesized and their crystal structures determined by single-crystal X-ray diffraction studies. The 2,6- dhb− ligand coordinates to the lanthanide cations through the carboxylate group in a monodentate or bidentate chelating coordination mode. The compounds were further characterised using IR, Raman, 1H and 13C NMR spectroscopy and elemental analysis. Photoluminescence measurements were performed on both compounds. In particular, the terbium complex 2 shows intense photoluminescence both in the solid state and in ethanolic solution. Compound 2 also exhibits triboluminescence

Terbiumpolyoxotungstate anions as building units to fabricate nanostructured films

Sousa FL, Soares-Santos PCR, Nogueira HIS, et al. Terbiumpolyoxotungstate anions as building units to fabricate nanostructured films. In: Vilarinho PM, ed. Advanced Materials Forum III, Pts 1 and 2. Material Science Forum. Vol 514. Trans Tech Publ.; 2006: 1135-1139.A layer-by-layer assembly method was employed to fabricate multilayered films containing the terbium heteropolyanions K-6[Tb(BW11O39)(H2O)(3)](.)32H(2)O and Ky[Tb(XW11O39)(2)](.)nH(2)O (X = Si and P) and the polyelectrolytes poly(4-styrenesulfonate) and poly(allylamine hydrochloride). A comparative study on the optical properties of the films containing distinct polyoxometalates and different types of multilayers was carried out. The photoluminescence of the solid polyoxometalates and of the resulting films was investigated

Crises hipertensivas em portadores de hipertensão arterial em tratamento ambulatorial Crisis hipertensivas en portadores de hipertensión arterial en tratamiento ambulatorio Hypertensive crises in bearers of arterial hypertension in ambulatorial treatment

O presente estudo avaliou as características sociodemográficas e de adesão terapêutica de 27 portadores de hipertensão arterial em tratamento ambulatorial que apresentaram crises de urgências ou emergências hipertensivas e haviam sido atendidos em uma unidade de leito-dia e em uma unidade de emergência da cidade de Fortaleza-Ceará, no período de outubro de 2002 a maio de 2003. A maioria era mulher, com idade de 50 a 60 anos, pouca escolaridade, tempo de tratamento inferior a cinco anos e tempo de diagnóstico entre cinco e dez anos. O uso dos remédios foi o tratamento mais referido, seguido pela redução do consumo de sal e comparecimento às consultas. No entanto, o fato de comparecerem às consultas e receberem orientação parece não modificar o comportamento, uma vez que a maioria dos entrevistados não praticava exercícios físicos e demonstrava deficiência no conhecimento sobre a doença, atribuindo a elevação da pressão arterial a fatores emocionais.<br>El presente estudio evaluó las características sociodemográficas y de adhesión terapéutica de 27 portadores de hipertensión arterial en tratamiento ambulatorio que presentaron crisis de urgencias o emergencias hipertensivas y habían sido atendidos en una unidad de internamiento diurno y en una unidad de emergencia de la ciudad de Fortaleza - Ceará, en el período de octubre del 2002 a mayo del 2003. La mayoría fue del sexo femenino, con edad de 50 a 60 años, poca escolaridad, tiempo de tratamiento inferior a cinco años y tiempo de diagnóstico entre cinco y diez años. El uso de los remedios fue el tratamiento más referido, seguido por la reducción del consumo de sal y asistencia a las consultas. Entre tanto, el hecho de asistir a las consultas y recibir orientación parece no modificar su comportamiento, pues la mayoría de los entrevistados no praticaba ejercicios físicos y demostraba falta de conocimiento sobre la enfermedad, atribuyendo la elevación de la presión arterial a factores emocionales.<br>This study assessed the socio-demographic characteristics and the characteristics of therapeutic adhesion of 27 bearers of arterial hypertension undergoing ambulatorial treatment who had hypertensive urgencies crises or emergencies in the city of Fortaleza in the period between October of 2002 and May of 2003. The majority were women, between 50 and 60-years old, with little formal education, treatment time shorter than 5 years and time of diagnosis varying from 5 to 10 years. The use of medicine was the treatment that was most mentioned, followed by the reduction of the consumption of salt and attendance to medical appointments. However, attending the appointments and receiving orientation did not seem to change their behavior, since most of the patients that were interviewed practiced no physical exercises and demonstrated little knowledge of the illness, for they attributed the rise of the arterial pressure to emotional factors

Automedicação na adolescência: um desafio para a educação em saúde Self-medication in adolescence: a challenge to health education

O estudo visa analisar o conhecimento dos estudantes de escolas públicas e privadas do município de Fortaleza (CE) sobre o uso de medicamentos e suas implicações para a saúde. Trata-se de um estudo descritivo, com 722 adolescentes matriculados em dez escolas do município de Fortaleza. Levantou-se, por meio de um questionário, aspectos relativos ao consumo, indicação e orientação de medicamentos e a influência da mídia. Relataram uso de medicamentos, nos últimos sessenta dias, 72,0% dos participantes, sendo os analgésicos os mais citados (65,4%). Os responsáveis pelas indicações foram familiares (51,2%) e médicos (33,1%), sendo a automedicação relatada por 20,8% dos estudantes. Não receberam orientações sobre o uso correto de medicamentos 70,9% dos alunos. Do total, 34,1% revelaram já terem sido influenciados por meios de comunicação na compra de medicamentos. É possível concluir que o conhecimento dos adolescentes sobre medicamentos e suas implicações na saúde é bastante incipiente e desprovido de qualquer noção básica sobre o uso racional deles. Além disso, os estudantes da rede pública são mais orientados sobre o uso correto de medicamentos, por parte dos profissionais do sistema público de saúde, o que demonstra a eficiência do sistema como promotor de saúde da população matriculada em escolas públicas de Fortaleza.<br>The study aims to examine the knowledge of students of public and private schools in the city of Fortaleza, Ceará State, Brazil on the use of medicine and their health implications. It is a descriptive study of 722 adolescents enrolled in ten schools in the city of Fortaleza. He got up aspects of consumption, indication and guidance medicine and influence of the media using a questionnaire. Seventy two percent of the participants reported use of medicine the last 60 days, and painkillers were the most cited (65.4%). The indications were responsible for the family (51.2%) and physicians (33.1%), and the self-medication by 20.8% of the students. Regarding the use of medicine 70.9% of the students were not instructed on the proper use of the medicine. Of the total, 34.1% had already been influenced by media in the purchase of medicine. It's possible to conclude that teenagers' knowledge about medicine and its implications on health is very weak and devoid of any basic notion of the rational use of medicine. In addition, public school students are more oriented on the proper use of medicines by professionals in the public health system, which demonstrates the efficiency of the promoter of health of the population enrolled in public schools in Fortaleza