452 research outputs found

    The influence of tree root water uptake on the long term hydrology of a clay fill railway embankment

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    This paper uses a numerical model to investigate the influence of tree root water uptake and tree removal on pore water pressures and the vertical movement of a clay fill railway embankment. Simulated results of soil wetting and drying are compared with field measurements from an instrumented railway embankment before and after tree removal. A parametric study compares the influence of vegetation on the seasonal movement of the embankment slope. The simulations and field measurements show that while trees cause significant seasonal variations in pore water pressure and water content near the soil surface, they can maintain persistent soil suctions at depth within the tree rooting zone. Demonstration of this result using a numerical model requires a root water uptake function that separates spatially the processes of water infiltration, evaporation and transpiration. When all of the trees are removed, the persistent soil suctions established by the trees are lost as water infiltrates from the soil surface. Leaving the trees in place over the bottom third of the slope can maintain persistent suctions at the slope toe, while potentially also reducing seasonal ground movements at the crest that may adversely affect railway track geometry

    Error in target-based georeferencing and registration in terrestrial laser scanning

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    Terrestrial laser scanning (TLS) has been used widely for various applications, such as measurement of movement caused by natural hazards and Earth surface processes. In TLS surveying, registration and georeferencing are two essential steps, and their accuracy often determines the usefulness of TLS surveys. So far, evaluation of registration and georeferencing errors has been based on statistics obtained from the data processing software provided by scanner manufacturers. This paper demonstrates that these statistics are incompetent measures of the actual registration and georeferencing errors in TLS data and, thus, should no longer be used in practice. To seek a suitable replacement, an investigation of the spatial pattern and the magnitude of the actual registration and georeferencing errors in TLS data points was undertaken. This led to the development of a quantitative means of estimating the registration- or georeferencing-induced positional error in point clouds. The solutions proposed can aid in the planning of TLS surveys where a minimum accuracy requirement is known, and are of use for subsequent analysis of the uncertainty in TLS datasets

    Mechanisms of root reinforcement in soils:An experimental methodology using four-dimensional X-ray computed tomography and digital volume correlation

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    Vegetation on railway or highway slopes can improve slope stability through the generation of soil pore water suctions by plant transpiration and mechanical soil reinforcement by the roots. To incorporate the enhanced shearing resistance and stiffness of root-reinforced soils in stability calculations, it is necessary to understand and quantify its effectiveness. This requires integrated and sophisticated experimental and multiscale modelling approaches to develop an understanding of the processes at different length scales, from individual root-soil interaction through to full soil-profile or slope scale. One of the challenges with multiscale models is ensuring that they sufficiently closely represent real behaviour. This requires calibration against detailed high-quality and data-rich experiments. This study presents a novel experimental methodology, which combines in situ direct shear loading of a willow root reinforced soil with X-ray computed tomography to capture the 3D chronology of soil and root deformation within the shear zone. Digital volume correlation (DVC) analysis was applied to the computed tomography (CT) dataset to obtain full-field 3D displacement and strain information. This paper demonstrates the feasibility and discusses the challenges associated with DVC experiments on root-reinforced soils

    Rapid South Atlantic spreading changes and coeval vertical motion in surrounding continents: Evidence for temporal changes of pressure-driven upper mantle flow

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    International audienceThe South Atlantic region displays (1) a topographic gradient across the basin, with Africa elevated relative to South America, (2) a bimodal spreading history with fast spreading rates in Late Cretaceous and Eo-Oligocene, and (3) episodic regional uplift events in the adjacent continents concentrated in Late Cretaceous and Oligocene. Here we show that these observations can be linked by dynamic processes within Earth's mantle, through temporal changes in asthenosphere flow beneath the region. The topographic gradient implies westward, pressure-driven mantle flow beneath the basin, while the rapid spreading rate changes, on order 10 million years, require significant decoupling of regional plate motion from the large-scale mantle buoyancy distribution through a mechanically weak asthenosphere. Andean topographic growth in late Miocene can explain the most recent South Atlantic spreading velocity reduction, arising from increased plate boundary forcing associated with the newly elevated topography. But this mechanism is unlikely to explain the Late Cretaceous/Tertiary spreading variations, as changes in Andean paleoelevation at the time are small. We propose an unsteady pressure-driven flow component in the asthenosphere beneath the South Atlantic region to explain the Late Cretaceous/Tertiary spreading rate variations. Temporal changes in mantle flow due to temporal changes in regional mantle pressure gradients imply a correlation of horizontal and vertical motions: we find that this prediction from our models agrees with geologic and geophysical observations of the South Atlantic region, including episodes of passive margin uplift, regional basin reactivation, and magmatic activity

    Modelling of stress transfer in root-reinforced soils informed by four-dimensional X-ray computed tomography and digital volume correlation data

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    Vegetation enhances soil shearing resistance through water uptake and root reinforcement. Analytical models for soils reinforced with roots rely on input parameters that are difficult to measure, leading to widely varying predictions of behaviour. The opaque heterogeneous nature of rooted soils results in complex soil-root interaction mechanisms that cannot easily be quantified. The authors measured, for the first time, the shear resistance and deformations of fallow, willow-rooted, and gorse-rooted soils during direct shear using X-ray computed tomography and digital volume correlation. Both species caused an increase in shear zone thickness, both initially and as shear progressed. Shear zone thickness peaked at up to 35 mm, often close to the thickest roots and towards the centre of the column. Root extension during shear was 10-30% less than the tri-linear root profile assumed in a Waldron-type model, owing to root curvature. Root analogues used to explore the root-soil interface behaviour suggested that root lateral branches play an important role in anchoring the roots. The Waldron-type model was modified to incorporate non-uniform shear zone thickness and growth, and accurately predicted the observed, up to seven-fold, increase in shear resistance of root-reinforced soil

    Mechanical and hydrological impacts of tree removal on a clay fill railway embankment

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    Seasonal shrinkage and swelling of clay fill railway embankments can disturb the track geometry, resulting in train speed restrictions that disrupt normal operations. Such movements are exacerbated by vegetation, but reliable analytical descriptions of the effects of trees on embankment behaviour are not yet established. This paper presents and analyses the results of a field experiment, carried out on a heavily vegetated clay railway embankment to investigate quantitatively the influence of trees. After the first year of monitoring, the mature trees initially present on the upper two-thirds of the embankment slopes were removed. The field monitoring data are used to assess and understand the mechanisms of soil water content and pore water pressure changes before and after tree removal, and their influence on the vertical and lateral displacements of the embankment slopes. Removal of the vegetation stopped seasonal volume changes in the clay fill at the crest of the earthwork, but also resulted in the loss of the deep-seated suctions generally beneficial to embankment stability. The wider implications for the management of vegetation on embankment slopes are discusse

    Current and future role of instrumentation and monitoring in the performance of transport infrastructure slopes

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    Instrumentation is often used to monitor the performance of engineered infrastructure slopes. This paper looks at the current role of instrumentation and monitoring, including the reasons for monitoring infrastructure slopes, the instrumentation typically installed and parameters measured. The paper then investigates recent developments in technology and considers how these may change the way that monitoring is used in the future, and tries to summarize the barriers and challenges to greater use of instrumentation in slope engineering. The challenges relate to economics of instrumentation within a wider risk management system, a better understanding of the way in which slopes perform and/or lose performance, and the complexities of managing and making decisions from greater quantities of data

    3D Growth of Cancer Cells Elicits Sensitivity to Kinase Inhibitors but Not Lipid Metabolism Modifiers

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    Tumor cells exhibit altered lipid metabolism compared with normal cells. Cell signaling kinases are important for regulating lipid synthesis and energy storage. How upstream kinases regulate lipid content, versus direct targeting of lipid-metabolizing enzymes, is currently unexplored. We evaluated intracellular lipid concentrations in prostate and breast tumor spheroids, treated with drugs directly inhibiting metabolic enzymes fatty acid synthase (FASN), acetyl-CoA carboxylase (ACC), diacylglyceride acyltransferase (DGAT), and pyruvate dehydrogenase kinase (PDHK), or cell signaling kinase enzymes PI3K, AKT, and mTOR with lipidomic analysis. We assessed whether baseline lipid profiles corresponded to inhibitors' effectiveness in modulating lipid profiles in three-dimensional (3D) growth and their relationship to therapeutic activity. Inhibitors against PI3K, AKT, and mTOR significantly inhibited MDA-MB-468 and PC3 cell growth in two-dimensional (2D) and 3D spheroid growth, while moderately altering lipid content. Conversely, metabolism inhibitors against FASN and DGAT altered lipid content most effectively, while only moderately inhibiting growth compared with kinase inhibitors. The FASN and ACC inhibitors' effectiveness in MDA-MB-468, versus PC3, suggested the former depended more on synthesis, whereas the latter may salvage lipids. Although baseline lipid profiles did not predict growth effects, lipid changes on therapy matched the growth effects of FASN and DGAT inhibitors. Several phospholipids, including phosphatidylcholine, were also upregulated following treatment, possibly via the Kennedy pathway. As this promotes tumor growth, combination studies should include drugs targeting it. Two-dimensional drug screening may miss important metabolism inhibitors or underestimate their potency. Clinical studies should consider serial measurements of tumor lipids to prove target modulation. Pretherapy tumor classification by de novo lipid synthesis versus uptake may help demonstrate efficacy
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