Systemic suppression of the shoot metabolism upon rice root nematode infection

Hirschmanniella oryzae is the most common plant-parasitic nematode in flooded rice cultivation systems. These migratory animals penetrate the plant roots and feed on the root cells, creating large cavities, extensive root necrosis and rotting. The objective of this study was to investigate the systemic response of the rice plant upon root infection by this nematode. RNA sequencing was applied on the above-ground parts of the rice plants at 3 and 7 days post inoculation. The data revealed significant modifications in the primary metabolism of the plant shoot, with a general suppression of for instance chlorophyll biosynthesis, the brassinosteroid pathway, and amino acid production. In the secondary metabolism, we detected a repression of the isoprenoid and shikimate pathways. These molecular changes can have dramatic consequences for the growth and yield of the rice plants, and could potentially change their susceptibility to above-ground pathogens and pests

Quantification of Nematic Cell Polarity in Three-dimensional Tissues

How epithelial cells coordinate their polarity to form functional tissues is an open question in cell biology. Here, we characterize a unique type of polarity found in liver tissue, nematic cell polarity, which is different from vectorial cell polarity in simple, sheet-like epithelia. We propose a conceptual and algorithmic framework to characterize complex patterns of polarity proteins on the surface of a cell in terms of a multipole expansion. To rigorously quantify previously observed tissue-level patterns of nematic cell polarity (Morales-Navarette et al., eLife 8:e44860, 2019), we introduce the concept of co-orientational order parameters, which generalize the known biaxial order parameters of the theory of liquid crystals. Applying these concepts to three-dimensional reconstructions of single cells from high-resolution imaging data of mouse liver tissue, we show that the axes of nematic cell polarity of hepatocytes exhibit local coordination and are aligned with the biaxially anisotropic sinusoidal network for blood transport. Our study characterizes liver tissue as a biological example of a biaxial liquid crystal. The general methodology developed here could be applied to other tissues or in-vitro organoids.Comment: 27 pages, 9 color figure

RAB25 expression is epigenetically downregulated in oral and oropharyngeal squamous cell carcinoma with lymph node metastasis

Oral and oropharyngeal squamous cell carcinoma (OOSCC) have a low survival rate, mainly due to metastasis to the regional lymph nodes. For optimal treatment of these metastases, a neck dissection is required; however, inaccurate detection methods results in under- and over-treatment. New DNA prognostic methylation biomarkers might improve lymph node metastases detection. To identify epigenetically regulated genes associated with lymph node metastases, genome-wide methylation analysis was performed on 6 OOSCC with (pN+) and 6 OOSCC without (pN0) lymph node metastases and combined with a gene expression signature predictive for pN+ status in OOSCC. Selected genes were validated using an independent OOSCC cohort by immunohistochemistry and pyrosequencing, and on data retrieved from The Cancer Genome Atlas. A two-step statistical selection of differentially methylated sequences revealed 14 genes with increased methylation status and mRNA downregulation in pN+ OOSCC. RAB25, a known tumor suppressor gene, was the highest-ranking gene in the discovery set. In the validation sets, both RAB25 mRNA (P = 0.015) and protein levels (P = 0.012) were lower in pN+ OOSCC. RAB25 mRNA levels were negatively correlated with RAB25 methylation levels (P < 0.001) but RAB25 protein expression was not. Our data revealed that promoter methylation is a mechanism resulting in downregulation of RAB25 expression in pN+ OOSCC and decreased expression is associated with lymph node metastasis. Detection of RAB25 methylation might contribute to lymph node metastasis diagnosis and serve as a potential new therapeutic target in OOSCC

Clifford-Jacobs Forging Company v. Capital Engineering & (and) Manufacturing Co.: The Continuing Problem within U.C.C. Section 2-207, 16 J. Marshall L. Rev. 589 (1983)

status: accepte

Horizontal Transfer of Symbiosis Genes within and Between Rhizobial Genera : Occurrence and Importance

Rhizobial symbiosis genes are often carried on symbiotic islands or plasmids that can be transferred (horizontal transfer) between different bacterial species. Symbiosis genes involved in horizontal transfer have different phylogenies with respect to the core genome of their ‘host’. Here, the literature on legume–rhizobium symbioses in field soils was reviewed, and cases of phylogenetic incongruence between rhizobium core and symbiosis genes were collated. The occurrence and importance of horizontal transfer of rhizobial symbiosis genes within and between bacterial genera were assessed. Horizontal transfer of symbiosis genes between rhizobial strains is of common occurrence, is widespread geographically, is not restricted to specific rhizobial genera, and occurs within and between rhizobial genera. The transfer of symbiosis genes to bacteria adapted to local soil conditions can allow these bacteria to become rhizobial symbionts of previously incompatible legumes growing in these soils. This, in turn, will have consequences for the growth, life history, and biogeography of the legume species involved, which provides a critical ecological link connecting the horizontal transfer of symbiosis genes between rhizobial bacteria in the soil to the above-ground floral biodiversity and vegetation community structure

Nanovezels voor drug delivery van slecht oplosbare medicijnen

Om een ziekte te bestrijden via orale medicatie, moet het actieve farmaceutische bestandsdeel (API) van het gebruikte medicijn oplosbaar zijn in de waterige omgeving van het menselijk lichaam. Bepaalde, dikwijls veelbelovende, API’s zijn echter slecht oplosbaar, wat vaak te wijten is aan de kristalliniteit van het API. In dit project werd de solvent elektrospintechniek aangewend om het kristallijne API in amorfe toestand te brengen als solid dispersion (SD). Uit verschillende analyses bleek dat de wateroplosbaarheid van het medicijn hierdoor drastisch verbetert. Het elektrospinproces is daardoor een potentieel veelbelovende techniek voor de verwerking van doeltreffende API’s in de farmaceutische industrie

Ultra faint dwarfs: probing early cosmic star formation

We investigate the nature of the newly discovered Ultra Faint dwarf spheroidal galaxies (UF dSphs) in a general cosmological context simultaneously accounting for various ``classical`` dSphs and Milky Way properties including their Metallicity Distribution Function (MDF). To this aim we extend the merger tree approach previously developed to include the presence of star-forming minihaloes, and an heuristic prescription for radiative feedback. The model successfully reproduces both the observed [Fe/H]-Luminosity relation and the mean MDF of UFs. In this picture UFs are the oldest, most dark matter-dominated (M/L > 100) dSphs with a total mass M= 10^{7-8}Msun; they are leftovers of H_2-cooling minihaloes formed at z > 8.5, i.e. before reionization. Their MDF is broader (because of a more prolonged SF) and shifted towards lower [Fe/H] (as a result of a lower gas metallicity at the time of formation) than that of classical dSphs. These systems are very ineffectively star-forming, turning into stars by z=0 only <3% of the potentially available baryons. We provide a useful fit for the star formation efficiency of dSphs.Comment: 5 pages, 4 figures, accepted for publication in MNRAS http://saveitalianbrains.wordpress.co

Basic science research opportunities in thrombosis and hemostasis : Communication from the SSC of the ISTH

ACKNOWLEDGMENTS We thank Drs. Hari Hara Sudhan Lakshmanan and Sven Olson for illustrative assistance and design.Peer reviewedPublisher PD

Host intestinal biomarker identification in a gut leakage model in broilers

International audienceAbstractIntestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance (p < 0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth (p < 0.0001) as well as higher infiltration of T-lymphocytes (p < 0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals (p < 0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals (p < 0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers