The role of somatic maturation on bioimpedance patterns and body composition in male elite youth soccer players

The purpose of this study was to examine the influence of chronological age (CA) and somatic maturation on body composition (BC) and bioimpedance parameters in male elite soccer players. BC and bioimpedance variables were measured in a sample of 249 players aged 9 1218 years of age and registered in two professional Italian soccer teams. Results from segmental analysis showed transition time points where the influence of CA and somatic maturation on bioimpedance patterns and BC characteristics increased or subsided. The accelerated phases were assessed for fat free mass, total body water, and upper muscle area, with a starting time point at approximately 122.00 years from peak at velocity (YPHV), and for body cell mass, whose developmental tempo sped up around 121.00 YPHV. An increase in the rate of development was also observed close to 122.00 YPHV for phase angle (PA), although without accelerated phases. From a CA point of view, significant slope changes were found for all BC and bioimpendance variables, except for the calf muscle area. Although the starting points and the span of the accelerated phases were different, they subsided or disappeared at ~15 years, except for PA, whose growth waned at ~17 years

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mature dendritic cells (mDCs) derived from porcine monocytes and matured with lipopolysaccharide. The infection of mDCs induced apoptosis, reduced the expression of CD80/86 and major histocompatibility complex class II molecules, and increased the expression of interleukin-10, thus suggesting that such mDC modulation results in the impairment of T-cell activation

Classic Bioelectrical Impedance Vector Reference Values for Assessing Body Composition in Male and Female Athletes

Bioimpedance standards are well established for the normal healthy population and in clinical settings, but they are not available for many sports categories. The aim of this study was to develop reference values for male and female athletes using classic bioimpedance vector analysis (BIVA). In this study, 1556 athletes engaged in different sports were evaluated during their off-season period. A tetrapolar bioelectrical impedance analyzer was used to determine measurements of resistance (R) and reactance (Xc). The classic BIVA procedure, which corrects bioelectrical values for body height, was applied, and fat-free mass, fat mass, and total body water were estimated. In order to verify the need for specific references, classic bioelectrical values were compared to the reference values for the general male and female populations. Additionally, athletes were divided into three groups: endurance, velocity/power, and team sports. In comparison with the general healthy male and female populations, the mean vectors of the athletes showed a shift to the left on the R-Xc graph. Considering the same set of modalities, BIVA confidence graphs showed that male and female endurance athletes presented lower body fluids, fat mass, and fat-free mass than other sets of modalities. This study provides BIVA reference values for an athletic population that can be used as a standard for assessing body composition in male and female athletes

The predictive role of raw bioelectrical impedance parameters in water compartments and fluid distribution assessed by dilution techniques in athletes

The aims of this study were to analyze the usefulness of raw bioelectrical impedance (BI) parameters in assessing water compartments and fluid distribution in athletes. A total of 202 men and 71 female athletes were analyzed. Total body water (TBW) and extracellular water (ECW) were determined by dilution techniques, while intracellular water (ICW) was calculated. Fluid distribution was calculated as the ECW/ICW ratio (E:I). Phase angle (PhA), resistance (R) and reactance (Xc) were obtained through BI spectroscopy using frequency 50kHz. Fat (FM) and fat\u2010free mass (FFM) were assessed by dual\u2010energy X\u2010ray absorptiometry. After adjusting for height, FM, FFM, age and sports category we observed that: PhA predicted ICW (females: \u3b2 = 1.62, p < 0.01; males: \u3b2 = 2.70, p < 0.01) and E:I (males and females: \u3b2 = 120.08; p < 0.01); R explained TBW (females: \u3b2 = 120.03; p < 0.01; males: \u3b2 = 120.06; p < 0.01) and ECW (females: \u3b2 = \u20130.02, p < 0.01; males: \u3b2 = 120.03, p < 0.01) and ICW (females: \u3b2 = \u20130.01, p < 0.053; males: \u3b2 = \u20130.03 p < 0.01); and Xc predicted ECW (females: \u3b2 = 120.06, p < 0.01; males: \u3b2 = 120.12, p < 0.01). A higher PhA is a good predictor of a larger ICW pool and a lower E:I, regardless of body composition, age, height, and sports category. Lower R is associated with higher water pools whereas ECW expansion is explained by lower Xc. Raw BI parameters are useful predictors of total and extracellular pools, cellular hydration and fluid distribution in athletes

An AFM Approach of RBC Micro and Nanoscale Topographic Features During Storage

Blood gamma irradiation is the only available method to prevent transfusion-associated graft versus host disease (TA-GVHD). However, when blood is irradiated, determine blood shelf time is crucial. Non-irradiated blood has a self-time from 21 to 35 days when is preserved with an anticoagulated solution and stored at 4°C. During their storage, red blood cells (RBC) undergo a series of biochemical, biomechanical and molecular changes involving what is known as storage lesion (SL). SL include loss of structural integrity of RBC, a decrease of 2,3-diphosphatidylglyceric acid levels, and an increase of both ion potassium concentration and hemoglobin (Hb). On the other hand, Atomic force Microscopy (AFM) represents a versatile tool for a nano-scale high-resolution topographic analysis in biological systems. In order to evaluate SL in irradiated and non-irradiated blood, RBC topography and morphometric parameters were obtained from an AFM XE-BIO system. Cell viability was followed using flow cytometry. Our results showed that early markers as nanoscale roughness, allow us to evaluate blood quality since another perspective

The open economy balance sheet channel and the exporting decisions of firms: evidence from the Brazilian crisis of 1999

We consider the impact of the Brazilian crisis of 1999 on the extensive and intensive margin of exporters versus non-exporters through the open economy balance sheet channel. Using an open economy balance sheet channel model with firm heterogeneity, we explore predictors that firms will engage in global markets. Our results based on a detailed firm-level panel of data for Brazilian 10,573 firms for the period 1996–2007, show that the decision to export and overall growth of sales for exporting firms is driven by size, the debt ratio, the current ratio and operating costs as well as the direct impact of the crisis itself. The findings suggest that the mechanism is driven by the response of the credit market to the creditworthiness of firms as it is by changing terms of trade

Physicochemical Characterization And Sources Of The Thoracic Fraction Of Road Dust In A Latin American Megacity

Road dust has been identified as one of the main sources of outdoor PM10 in Bogota (a Latin American megacity), but there are no studies that have analyzed the physicochemical characteristics and origins of its respirable fraction. A characterization of inorganic compounds (water soluble ions, major and trace elements, organic and elemental carbon) and an analysis of source contributions to the PM10 fraction of road dust were carried out in this study. A total of twenty road dust samples, selected from representative industrial, residential and commercial areas, were swept and resuspended to obtain the thoracic fraction. Size distribution by laser diffraction and individual particle morphology by Scanning Electron Microscopy were also evaluated. The data obtained revealed that the volume (%) of thoracic particles was higher in samples from industrial zones where heavy vehicular traffic, industrial emissions and deteriorated pavements predominated. Crustal elements were the most abundant species, accounting for 49–62% of the thoracic mass, followed by OC (13–29%), water-soluble ions (1.4–3.8%), EC (0.8–1.9%) and trace elements (0.2–0.5%). The Coefficient of Divergence was obtained to identify the spatial variability of the samples. A source apportionment analysis was carried out considering the variability of chemical profiles, enrichment factors and ratios of Fe/Al, K/Al, Ca/Al, Ti/Al, Cu/Sb, Zn/Sb, OC/TC and OC/EC. By means of a PCA analysis, five components were identified, including local soils and pavement erosion (63%), construction and demolition activities (13%), industrial emissions (6%), brake wear (5%) and tailpipe emissions (4%). These components accounted for 91% of the total variance. The results provide data to understand better one of the main sources of PM10 emissions in Bogota, such as road dust. These data will be useful to optimize environmental policies, and they may be used in future studies of human health and air quality modeling

New alkaloid antibiotics that target the DNA topoisomerase I of Streptococcus pneumoniae

Streptococcus pneumoniae has two type II DNA-topoisomerases (DNA-gyrase and DNA topoisomerase IV) and a single type I enzyme (DNA-topoisomerase I, TopA), as demonstrated here. Although fluoroquinolones target type II enzymes, antibiotics efficiently targeting TopA have not yet been reported. Eighteen alkaloids (seven aporphine and 11 phenanthrenes) were semisynthesized from boldine and used to test inhibition both of TopA activity and of cell growth. Two phenanthrenes (seconeolitsine and N-methyl-seconeolitsine) effectively inhibited both TopA activity and cell growth at equivalent concentrations (∼17 μM). Evidence for in vivo TopA targeting by seconeolitsine was provided by the protection of growth inhibition in a S. pneumoniae culture in which the enzyme was overproduced. Additionally, hypernegative supercoiling was observed in an internal plasmid after drug treatment. Furthermore, a model of pneumococcal TopA was made based on the crystal structure of Escherichia coli TopA. Docking calculations indicated strong interactions of the alkaloids with the nucleotide-binding site in the closed protein conformation, which correlated with their inhibitory effect. Finally, although seconeolitsine and N-methyl-seconeolitsine inhibited TopA and bacterial growth, they did not affect human cell viability. Therefore, these new alkaloids can be envisaged as new therapeutic candidates for the treatment of S. pneumoniae infections resistant to other antibiotics.The work was supported by Comunidad de Madrid Grant CM-BIO0260-2006, COMBACT (to J. H. and A. G. C.); Spanish Ministry of Science and Innovation Grants BIO2008-02154 (to A. G. C.), BFU2008-01711 (to J. A. H.), and SAF2008-03477 (to M. J. S.); and Spanish Ministry of Health, Carlos III Health Institute Grants RIER, RD08/0075/0016 (to M. J. S.).S

Specific Bioelectrical Impedance Vector Analysis Identifies Body Fat Reduction after a Lifestyle Intervention in Former Elite Athletes

Background: specific bioelectrical impedance vector analysis (BIVA) has been proposed as an alternative bioimpedance method for evaluating body composition. This investigation aimed to verify the ability of specific BIVA in identifying changes in fat mass after a 16-week lifestyle program in former athletes. Methods: The 94 participants included in the Champ4life project (clinicaltrials.gov: NCT03031951) were randomized into intervention (n = 49) and control (n = 45) groups, from which 82 athletes completed the intervention (age 43.9 ± 9.2 y; body mass index 31.1 ± 4.6 kg/m2). Fat mass was estimated by dual-energy X-ray absorptiometry. Bioelectric resistance, reactance, phase angle, and vector length were assessed by bioelectric impedance spectroscopy, and the BIVA procedure was applied. Results: A significant (p < 0.05) group x time interaction for fat mass, specific resistance, reactance, and vector length was found. Fat mass and vector length significantly (p < 0.05) decreased in the intervention group, while no change was measured in the control group. Considering the participants as a whole group, changes in vector length were associated with changes in fat mass percentage (r2 = 0.246; β = 0.33; p < 0.001) even after adjusting for age, sex, and group (R2 = 0.373; β = 0.23; p = 0.002). Conclusions: The specific BIVA approach is suitable to track fat mass changes during an intervention program aimed to reduce body fat in former athletes