Assessment of the phenotypic effects of Platelet Rich Fibrin on Mesenchymal Stem Cells derived from Minced Pulp

Our aim is to investigate the effects of autologous platelet-rich fibrin (PRF) on Mesenchymal Stem cell derived from Minced Pulp (MP-MSCs). We first developed a mouse model of PRF to study the phenotypic effects of PRF in cultured cells. We obtained PRF from the blood and prepared PRF-enriched culture media. The phenotypic effects of PRF on MP-MSCs were determined by assessing the changes in cell proliferation, differentiation and immunophenotypic profiling. The mRNA levels of ALP, OCN, DMP1 and DSPP were determined by qRT-PCR. It was found that PRF increased the proliferation capacity of MP-MSCs and reduced the cell doubling time. With PRF exposure, the MP-MSCs were able to retain their immunophenotypic characteristics defining them as MSCs, as the cells expressing surface markers CD105, CD146 and CD73 were higher. MP-MSCs were able to undergo osteogenic differentiation in the presence of PRF and the mRNA levels of OCN was significantly increased in the presence of PRF. To assess the odontogenic differentiation of cells in response to PRF, we prepared dentin-slice model in which we cultured MP-MSCs embedded in PRF. Histological sections of the dentin slice model revealed that there was increase in the cellularity of the pulp tissue along the edges of the pulp tissue. Based on our findings, PRF can act as a source of growth factors cell proliferation, migration and differentiation

The IntAct database:Efficient access to fine-grained molecular interaction data

The IntAct molecular interaction database (https://www.ebi.ac.uk/intact) is a curated resource of molecular interactions, derived from the scientific literature and from direct data depositions. As of August 2021, IntAct provides more than one million binary interactions, curated by twelve global partners of the International Molecular Exchange consortium, for which the IntAct database provides a shared curation and dissemination platform. The IMEx curation policy has always emphasised a fine-grained data and curation model, aiming to capture the relevant experimental detail essential for the interpretation of the provided molecular interaction data. Here, we present recent curation focus and progress, as well as a completely redeveloped website which presents IntAct data in a much more user-friendly and detailed way

Balancing Social and Political Strategies in Emerging Markets: Evidence from India

This article explores the substitution and complementary effects between political and social strategies on firm performance in the context of an emerging market (EM). Using in-depth, historical case-study approach, the article investigates how companies integrate political and social resources in this market. Corporate performance includes traditional measures such as accounting performance and nonfinancial measures like the ease of doing business. The study finds that social strategies are stronger enablers of firm long-term performance than political strategies. The latter have a short-term impact on performance, but their success over time is limited. The main drawback of reliance on political resources in EMs is the lack of political stability, fragmented polity, and weak political coalitions. We identify rather limited evidence of firms using these two strategies as complements. Thus, we suggest that firms should employ both these strategies in the EM

Assessment of the phenotypic effects of Platelet Rich Fibrin on Mesenchymal Stem Cells derived from Minced Pulp

Our aim is to investigate the effects of autologous platelet-rich fibrin (PRF) on Mesenchymal Stem cell derived from Minced Pulp (MP-MSCs). We first developed a mouse model of PRF to study the phenotypic effects of PRF in cultured cells. We obtained PRF from the blood and prepared PRF-enriched culture media. The phenotypic effects of PRF on MP-MSCs were determined by assessing the changes in cell proliferation, differentiation and immunophenotypic profiling. The mRNA levels of ALP, OCN, DMP1 and DSPP were determined by qRT-PCR. It was found that PRF increased the proliferation capacity of MP-MSCs and reduced the cell doubling time. With PRF exposure, the MP-MSCs were able to retain their immunophenotypic characteristics defining them as MSCs, as the cells expressing surface markers CD105, CD146 and CD73 were higher. MP-MSCs were able to undergo osteogenic differentiation in the presence of PRF and the mRNA levels of OCN was significantly increased in the presence of PRF. To assess the odontogenic differentiation of cells in response to PRF, we prepared dentin-slice model in which we cultured MP-MSCs embedded in PRF. Histological sections of the dentin slice model revealed that there was increase in the cellularity of the pulp tissue along the edges of the pulp tissue. Based on our findings, PRF can act as a source of growth factors cell proliferation, migration and differentiation