ISOLATION OF Salmonella typhoid 16s rRNA GENE FRAGMENT BASED ON POLYMERASE CHAIN REACTION (PCR)

Iridescent is one of the traditional fish fermentation products that has a sour taste and is widely known in various regions of Indonesia. In its metabolism LAB produces organic acids, hydrogen peroxide, diacetyl, CO2 and bacteriocin. These compounds are antimicrobial compounds that inhibit the growth of pathogenic bacteria and bacterial decomposition. The purpose of this study was to determine the antibacterial activity produced by lactic acid bacteria isolates from catfish (Pangasius hypophthalmus) inhibiting the growth of Escherecia coli. From iridescent obtained 9 isolates that have different characteristics from each other by the morphology of LAB isolate colonies which include colony color, colony shape, colony elevation and colony margin. After production of antibacterial, all isolates showed positive result when antibacterial tested againts Escherecia coli either in acidic condition or neutralized

Pelayanan Pembuatan Akta Kelahiran Di Kabupaten Subang

According to the data gained by researchers from the Department of Population and Civil Registration in Subang, there are still the number of children aged 0 to 18 years who do not owna Birth Certificate. In 2018, the Subang Population and Civil Registry Service set a target of 85%, where children in Subang Ages 0 to 18 years must own a Birth Certificate. However, there are lots of obstacles to realizing this target, both from service and the community. The research method used in this study is qualitative approach. In this study, researchers used a descriptive research type so that it would be described how effective the service of making Birth Certificates for Children aged 0 to 18 years in the Population and Civil Registry Office of Subang. In order to get data and information, The techniques that the researchers did are through observation and interviews. The results of the research in the field show that the effectiveness of birth certificate making services in the context of fulfilling the identity of children aged 0 to 18 years in Subang in terms of service is very optimal but there are obstacles from the community

A Contextual Semi-Assisted Project-Based Learning on Ocean Wave Energy: Pre-service Physics Teachers’ Perceptions

The lack of technological innovation in the utilization of ocean wave energy as a renewable energy source is caused by a lack of public knowledge about this matter. Therefore, it needs to be introduced early, especially to pre-service teachers through innovative and fun learning. The aim of this study was to identify the responses of pre-service physics teachers about the learning program to introduce the concept of utilizing ocean wave energy. The research subjects were 31 sixth semester students who are taking environmental physics lectures in the physics education study program at one of the teachers' university in West Nusa Tenggara Province for the 2019/2020 academic year. The sample was taken by using purposive sampling technique. Data collection was carried out by means of observation, interview, and questionnaire techniques. All data collected were then analyzed simultaneously descriptively. The findings of this study showed that almost 90% pre-service physics teacher students agreed that the learning program had a positive impact on increasing interest, motivation, critical thinking skills, and creative thinking skills, and made them easier to solve problems and grow solid cooperation. Therefore, it can be concluded that pre-service physics teacher students agree to the use of semi-assisted project-based learning programs with contextual problems

Detection of Luminous Vibrio harveyi in Penaeid Shrimp Through Nested PCR Using Haemolysin Gene Primer

Whiteleg shrimp (Litopenaeus vannamei)  is one of the most important aquaculture commodity in Indonesia. However, the luminous disease primarily caused by Vibrio harveyi bacteria still becomes an obstacle in penaeid shrimp farming, especially in shrimp hatchery. This study was aimed to identify the presence of V. harveyi in L. vannamei through nested PCR using haemolysin gene primer. First, initial primers were designed using V. harveyi VIB 391 haemolysin gene sequence (accession number: DQ640264), flanking the position 133 to 756. This primer pairs were used to identify haemolysin gene in both V. harveyi MR5339 and V. harveyi 275 strain. Sequencing results from each sample showed 99% similarity with haemolysin gene sequence in Genebank. Furthermore, the sequence of V. harveyi MR5339 haemolysin gene was used to design the nested PCR primers. The first primer pairs of nested PCR have successfully amplified the haemolysin gene fragment of all V. harveyi strains samples from position 52 to 405. The second primer pairs of nested PCR have amplified position 204 to 405 where it can detect all of V. harveyi strains used as sample sources in this study. The application of nested PCR technique in this study was able to identify V. harveyi strains at serial dilution of cells density as low as 100 cfu/mL, which is equal to a single cell or at DNA concentration up to 101 fg/µL

A contextual semi assisted project-based learning (SA-PjBL) about ocean wave energy: Creative thinking of pre-service physics teachers

One of the factors that have become an obstacle in the development of techno-logy to use ocean wave energy as a renewable energy source is the lack of human resources who have expertise in this field. Therefore, in the long term, it is necessary to introduce basic knowledge to the young generation about wave energy and other renew-able energies and even must equip them with important skills such as creative thinking skills. This study aims to introduce students to how to utilize ocean waves as renewable energy while at the same time developing student creative thinking skills through semi-assisted project-based learning. This type of research is weak experiment research with one group pretest-posttest design research. The research was conducted on 31 pre-service physics physics teachers who were taking environmental physics lectures at a teacher training university in West Nusa Tenggara. The performance appraisal data were analyzed descriptively while the test result data were analyzed using the N-gain and the Wilcoxon test. Based on the results of data analysis, it was obtained <g> = 0.725, which means that there was a high increase in students' mastery of the concept of using ocean waves as a renewable energy source. In addition, the results of the performance appraisal data (score > 3) indicated that at each stage of learning, students were provided with indicators of creative thinking skills with very satisfied categories. Therefore, it can be concluded that environmental physics lectures using semi-assisted project-based learning able to improve students' mastery of concepts and creative thinking skills

RESISTANCE OF RED CURLY CHILI (Capsicum annuum L.) SPROUTS TO FUSARIUM OXYSPORUM INFECTION FROM SEEDS INDUCED BY 0.2 mT

Curly red chili (Capsicum annuum L.) is widely used as industrial raw material because it has a spicy taste. The increasing demand for chili is not balanced with the level of production. Chilies are susceptible to disease, one of which is fusarium wilt. The magnetic field affected the physical and chemical properties of water so that it increased peroxidase enzyme activity and plant metabolism. This research was aim to know the protective effect seeds induced by 0.2 mT Magnetic Fiel had given to red curly chili (Capsicum annuum L.) sprouts to Fusarium oxysporum infection. This study was arranged in a completely randomized design (CRD) with 5 treatments consisting of M0F0 (control), M7F0, M7F60, M15F0, M15F60. M0 is a seed not induced by a magnetic field; M7 is seed induced by magnetic field 7 minutes 48 seconds, M15 is seed induced by magnetic field 15 minutes 36 seconds, F0 is sprouted not infected with F. oxysporum and F60 is sprouts infected with F. oxysporum for 60 minutes. Each unit is repeated 5 times. The results of the ANOVA showed that exposure to a magnetic field had a significant effect on increasing plant height at 21, 28, and 35 days after planting (HST); the wet and dry weight of 7 days old plants; and the content of chlorophyll a, b, and total before flowering 21 days after planting. Overall, the 0.2 mT magnetic field treatment for 7 minutes 48 seconds tended to give better results to increase the growth of chili plants against F. oxysporum infection

Pertumbuhan Benih Cabai (Capsicum annuum L.) dengan Induksi Medan Magnet 0,2 mT dan Infeksi Fusarium oxysporum

Medan magnet merupakan salah satu upaya yang dapat digunakan untuk mengendalikan penyakit layu fusarium pada tanaman cabai (Capsicum annuum L.). Pengendalian yang diharapkan tidak berdampak negatif terhadap manusia dan hewan serta ramah lingkungan. Penelitian ini bertujuan untuk mengetahui pengaruh lama paparan medan magnet 0,2 mT (M) dan infeksi Fusarium oxysporum  (F) terhadap pertumbuhan kecambah cabai. Penelitian ini disusun menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan antara lain M0F0, M7F0, M15F0, M0F60, M7F60, dan M15F60. Benih cabai diinfeksi dengan Fusarium oxysporum selama 0 menit (F0) dan 60 menit (F60). Data yang diperoleh dilakukan analisis ragam, apabila perlakuan memberikan pengaruh terhadap parameter yang diukur, akan dilanjutkan dengan Uji BNT 5%. Hasil penelitian menunjukkan bahwa aktivitas enzim α-amilase tertinggi terdapat pada M15F60 sebesar 2,78 U/mL pada benih dan 5,28 U/mL pada kecambah. Indeks vigor memiliki korelasi positif terhadap tinggi kecambah. Semakin meningkatnya indeks vigor maka semakin meningkat pula tinggi kecambah. Indeks vigor tertinggi diperoleh pada hari ke-6 dengan perlakuan M7F60 sebesar 4,04. Tinggi kecambah tertinggi diperoleh pada hari ke-9 dengan perlakuan yang sama seperti indeks vigor yaitu M7F60 sebesar 2,04 cm

ISOLASI BAKTERI FOTOSINTETIK DARI DASAR TAMBAK PADA KONDISI UDARA YANG MENGANDUNG ASAP KENDARAAN BERMOTOR

Semakin meningkatnya volume kendaraan saat ini berdampak pada meningkatnya polusi asap kendaraan. Asap kendaraan bermotor juga diketahui mengandung CO2 yang mampu meningkatkan pemanasan global. Diketahui bahwa terdapat bakteri fotosintetik yang mampu memfiksasi CO2. Bakteri ini dapat ditemukan di danau, sawah, maupun dasar tambak. Penelitian ini bertujuan untuk mengisolasi bakteri fotosintetik dari berasal dari dasar tambak setelah diberi penambahan asap kendaraan bermotor pada medium kulturnya. Ditemukan terdapat 5 isolat bakteri yang tumbuh; 3 bakteri bersifat gram negatif, 2 berbentuk batang dan 1 berbentuk bulat. 2 bakteri bersifat gram positif dengan bentuk batang

Metode Analytical Hierarchy Process (AHP) untuk Pemilihan Media Sosial Pemasaran Songket Sambas

Songket Sambas (Kain Lunggi) is a gold thread cloth typical of the culture of the Sambas people. The Sambas songket craftsmen have used social media a lot in marketing their products. The selection of the right social media in product marketing can help increase sales of Sambas songket. It can be the key to success in expanding Sambas songket sales to various regions. This study aims to apply the Analytical Hierarchy Process (AHP) method in the selection of social media that is widely used as the right Sambas songket marketing media. In this study, the AHP method was used as a method for decision making. In determining the pairwise comparison matrix, Sambas songket craftsmen were involved. The criteria used are age range (C1), gender (C2), number of users (C3), level of popularity (C4). From this study, we have the value of Facebook is 0.82, Instagram is 0.60, Youtube is 0.06, Twitter is 0.04. The final priority result of the right social media to use with the first order is Facebook

Antifungal Agent Chitooligosaccharides Derived from Solid-State Fermentation of Shrimp Shell Waste by Pseudonocardia antitumoralis 18D36-A1

Shrimp shell waste is a potential source of the biopolymer chitin. Through fermentation, chitin can be converted into its derivative products. This study aimed to isolate and characterize the products of the biodegradation of chitin from shrimp shell waste through a solid-state fermentation process using actinomycetes. Actinomycete isolates were obtained from tunicate marine biota collected from the waters of Buleleng, Bali, using a dilution technique on 1% chitin colloid agar medium. The isolated actinomycetes were cultivated on a shrimp shell waste medium for 7 days, and then the products of the biodegradation of the oligomers were extracted using water. The extracts of the biodegradation products of the shrimp shells were isolated through several chromatographic steps and analyzed using LC–MS–MS, and the bioactivity of the biodegradation products against fungi was tested. The morphological observations and phylogenetic analysis showed that the isolate 18D36-A1 was a rare actinomycete with the proposed name Pseudonocardia antitumoralis 18D36-A1. The results of the analysis using TLC showed that the solid-state fermented water isolate 18D36-A1 produced several oligomeric components. These results indicate that the isolate 18D36-A1 was able to convert chitin into chitooligosaccharides. Further isolation of the extract produced the active fraction D36A1C38, which can inhibit the growth of fungi by 74% at a concentration of 1 mg/mL. This initial information is very important for further studies related to the development of a solid-state fermentation process for obtaining bioactive compounds from shrimp shell waste