420 research outputs found
La investigación hidrogeomorfológica en cuencas experimentales de montaña media en el Pirineo Central (1996-2014)
10 páginasPeer reviewe
Análisis de la variabilidad temporal de la infiltración en un gradiente de degradación de usos del suelo en el Pirineo central
20 págs, 4 figs, 5 tablas[ES] Se analiza
la
capacidad
de
infiltración
con
respecto
a
las
condiciones
físicas
superficiales
(humedad
y
resistencia
mecánica)
de
suelos
en
el
Pirineo
Central,
considerando tres
escenarios:
bosque
natural,
campos
abandonados
y
cárcavas.
El
estudio
se
ha
realizado
en
tres
cuencas
experimentales
(San
Salvador,
Arnás
y
Araguás)
situadas
en
un
contexto
geográfico,
geológico
y
climático
similar.
La
información
se
ha
obtenido
mediante
ensayos
con
un
infiltrómetro
de
doble
anillo
entre
otoño
de
2008
y
verano
de
2009.
Los
resultados
sugieren
cierto
efecto
de
las
condiciones
físicas
previas
sobre
la capacidad
de
infiltración.
Sin
embargo,
el
uso
del
suelo
y
la orientación
de
las
laderas
(N‐S) se
han
revelado
los factores más
determinantes.
Esto
se
relaciona
con
la
variabilidad
estacional del
estado
físico
antecedente
y
su
dinámica
durante
los
ensayos.
Las
diferencias
de la
infiltración
asociada
a
la
orientación
de
las
laderas
incluso
pueden
superar
a
las
observadas
entre
usos
del
suelo.[EN] The
infiltration
capacity
has
been
analysed
related
to
the
variation
of
surface
physical
conditions
(moisture
and
mechanical
r esistance)
of
soils
from
the
Central
Spanish
Pyrenees,
taking
into
account
the
most
representative
situations
of
land
uses
and
vegetal
covers.
The
study
was
carried
out
in
three
experimental
catchments,
each
representative
of
different
environments
in
terms
of
soil
use
and
vegetation
cover:
forested
(San
Salvador),
field
abandonment
(Arnás)
and
badlands
(Araguás).
The
three
catchment s
are
located
near by
and
have
similar
geologica l
and
climatic
conditions.
The
study
was
done
through
experimental
infiltration
s et s
using
a
big
double
ring
instrument
(50
cm
water
column
height.
Experiments
were
performed
from
autumn
2008
to
summer
2009.
The
infiltration
process
was
analysed
on
six
land
uses
situations:
bare regolith,
grass
c overed
regolith,
north‐facing
slope
in
forested
soil,
south‐facing
slope
in
forested soil,
north‐facing
slope
in
meadow
soil
and
south‐facing
slope
in
pasture
soil.
Before and
after
each
experiment
soil
was
sampled
in
order
to
estimate
moisture
content
(0‐1,
0‐5
and
5‐10
cm
depth)
and
surface
mechanical
resistance
was
measured
with
the
help
of
a
pocket
penetrometer,
providing
an
idea
a bout
previous
conditions
and
its
variation.
The
results
suggest
a
certain
effect
of
moisture
conditions
on
infiltration
capacity.
However,
soil
use
and
slope
exposition
(North
and
South)
seem
to
be
the
most
determinant
factors.
This
is
related
to
the
seasonal
variability
of antecedent
soil
conditions
and
its
dynamics
during
the
experiments.
The
differences
on
infiltration
capacity
associated
with
slope
e xposition
can
be
higher
than
those
observed
between
land
uses.Este
estudio
se
ha financiado
mediante
fondos
del
proyecto
CETSUS
(CGL2007‐66644‐C04‐01/HID)
del
Plan
Nacional
I+D+I
2004‐207
(Ministerio
de
Ciencia
e
Innovación).
El
mantenimiento
y
parte
de
la
monitorización
de
las
cuencas
experimentales
se
ha
logrado
a
partir
de
la
financiación
proporcionada
por
el
convenio
RESEL (Ministerio
del
Medio
Ambiente‐CSIC). E.
Nadal‐Romero
y
N.
Lana‐Renault
contaron
durante
2009
y
2010
con
un
contrato postdoctoral
del
Ministerio
de
Ciencia
e
Innovación,
mediante
el
Programa
Nacional
de
Movilidad
de Recursos
Humanos
del
Plan
nacional
de
I‐D+I
2008‐2011
y
en
la
actualidad
están
contratadas
gracias
al
programa
Postdoctoral
Juan
de
la
Cierva
(Ministerio
de
Ciencia
e
Innovación).Peer reviewe
Seasonal differences in runoff between forested and non-forested catchments: a case study in the Spanish Pyrenees
[EN] The hydrological response of two neighbouring catchments in the central Spanish Pyrenees with similar lithology and topography but different land use was compared. One catchment (2.84 km 2 ) was extensively cultivated in the past, and the other (0.92 km 2 ) is covered by dense natural forest. Differences in runoff were strongly related to catchment wetness conditions and showed a marked seasonality: under dry conditions runoff tended to be greater in the former agricultural catchment, whereas under wet conditions it tended to be greater in the forested catchment. One explanation for this switching behaviour could be an increase in the hydrological connectivity within the slopes of the forested catchment as it becomes wetter, which favours the release of large amounts of subsurface flow. Differences in land use (vegetation and soil properties) dictate the contrasting dominant runoff generation processes operating in each catchment, and consequently the differences between their hydrological responses. Key words water yield; seasonal controls; hydrograph characteristics; forestSupport for this research was provided by the following projects: PROBASE (CGL2006-11619/HID), RespHiMed (CGL2010-18374) and MONTES (CSD2008-00040), financed by the Spanish Commission of Science and Technology; ACQWA (FP7-ENV-2007-1), financed by the European Commission; and PI032/08, financed by the Aragón Regional Government. The authors also acknowledge support from RESEL (the Spanish Ministry of the Environment). N. Lana-Renault was the recipient of a research contract (Juan de la Cierva programme) and J. Latron the recipient of a research contract (Ramón y Cajal programme), both funded by the Spanish Ministry of Sciences and Innovation.Peer Reviewe
Compatison of S-SI, A-SI and CDTE technologies wording at the same conditions, after the first year of electricity production
Grid connected solar plants are a good opportunity for their use for research as a secondary objective. In countries were feed-in tariffs are still active, it is possible to include in the design of the solar plant elements for its use for research. In the case of the solar plant presented here both objectives are covered. The solar plant of this work is formed by PV modules of three different technologies: Multicrystalline, amorphous and CdTe. In one part of the solar plant, the three technologies are working at the same conditions, not only ambient conditions but also similar voltage and current input to the inverters. Both the commercial and the experimental parts of the solar plant have their own independent inverters with their meters but are finally connected to the same meter to inject. In this work we analyse the results for the first year of operation of the experimental solar plant. Productions of three different technologies in exactly the same conditions are compared and presented. According to the results, all the three technologies have conversion efficiencies dropping when the temperature increases. Amorphous module experiences the lesser reduction, whereas the multicrystalline module suffers the most
Born too early and too small: higher order cognitive function and brain at risk at ages 8–16
Prematurity presents a risk for higher order cognitive functions. Some of these deficits
manifest later in development, when these functions are expected to mature. However,
the causes and consequences of prematurity are still unclear. We conducted a
longitudinal study to first identify clinical predictors of ultrasound brain abnormalities in
196 children born very preterm (VP; gestational age 32 weeks) and with very low birth
weight (VLBW; birth weight 1500 g). At ages 8–16, the subset of VP-VLBW children
without neurological findings (124) were invited for a neuropsychological assessment
and an MRI scan (41 accepted). Of these, 29 met a rigorous criterion for MRI quality
and an age, and gender-matched control group (n = 14) was included in this study.
The key findings in the VP-VLBW neonates were: (a) 37% of the VP-VLBW neonates
had ultrasound brain abnormalities; (b) gestational age and birth weight collectively with
hospital course (i.e., days in hospital, neonatal intensive care, mechanical ventilation and
with oxygen therapy, surgeries, and retinopathy of prematurity) predicted ultrasound
brain abnormalities. At ages 8–16, VP-VLBW children showed: a) lower intelligent
quotient (IQ) and executive function; b) decreased gray and white matter (WM) integrity;
(c) IQ correlated negatively with cortical thickness in higher order processing cortical
areas. In conclusion, our data indicate that facets of executive function and IQ are the
most affected in VP-VLBW children likely due to altered higher order cortical areas and
underlying WMThis study was supported by the Spanish Government Institute Carlos III (FIS Pl11/02860), Spanish Ministry of Health to MM-L, and the University of Castilla-La Mancha mobility Grant VA1381500149
Una especie nueva de avispa gallícola para México: Andricus sphaericus Pujade-Villar n. sp. (Hymenoptera: Cynipidae: Cynipini)
Se describe una nueva especie de cinípido gallícola de México, Andricus sphaericus Pujade-Villar n. sp., solo conocida por su forma sexual. Induce agallas en las hojas de Quercus rugosa Née. Representa la segunda colecta de una generación sexual del género Andricus para México. Se proporcionan datos referidos a la diagnosis, distribución y biología de esta nueva especie
Cyclic multiplex fluorescent immunohistochemistry and machine learning reveal distinct states of astrocytes and microglia in normal aging and Alzheimer’s disease
Background
Astrocytes and microglia react to Aβ plaques, neurofibrillary tangles, and neurodegeneration in the Alzheimer’s disease (AD) brain. Single-nuclei and single-cell RNA-seq have revealed multiple states or subpopulations of these glial cells but lack spatial information. We have developed a methodology of cyclic multiplex fluorescent immunohistochemistry on human postmortem brains and image analysis that enables a comprehensive morphological quantitative characterization of astrocytes and microglia in the context of their spatial relationships with plaques and tangles.
Methods
Single FFPE sections from the temporal association cortex of control and AD subjects were subjected to 8 cycles of multiplex fluorescent immunohistochemistry, including 7 astroglial, 6 microglial, 1 neuronal, Aβ, and phospho-tau markers. Our analysis pipeline consisted of: (1) image alignment across cycles; (2) background subtraction; (3) manual annotation of 5172 ALDH1L1+ astrocytic and 6226 IBA1+ microglial profiles; (4) local thresholding and segmentation of profiles; (5) machine learning on marker intensity data; and (6) deep learning on image features.
Results
Spectral clustering identified three phenotypes of astrocytes and microglia, which we termed “homeostatic,” “intermediate,” and “reactive.” Reactive and, to a lesser extent, intermediate astrocytes and microglia were closely associated with AD pathology (≤ 50 µm). Compared to homeostatic, reactive astrocytes contained substantially higher GFAP and YKL-40, modestly elevated vimentin and TSPO as well as EAAT1, and reduced GS. Intermediate astrocytes had markedly increased EAAT2, moderately increased GS, and intermediate GFAP and YKL-40 levels. Relative to homeostatic, reactive microglia showed increased expression of all markers (CD68, ferritin, MHC2, TMEM119, TSPO), whereas intermediate microglia exhibited increased ferritin and TMEM119 as well as intermediate CD68 levels. Machine learning models applied on either high-plex signal intensity data (gradient boosting machines) or directly on image features (convolutional neural networks) accurately discriminated control vs. AD diagnoses at the single-cell level.
Conclusions
Cyclic multiplex fluorescent immunohistochemistry combined with machine learning models holds promise to advance our understanding of the complexity and heterogeneity of glial responses as well as inform transcriptomics studies. Three distinct phenotypes emerged with our combination of markers, thus expanding the classic binary “homeostatic vs. reactive” classification to a third state, which could represent “transitional” or “resilient” glia.España Ministry of Science, Innovation, and Universities FPU fellowship to CM-CMassachusetts Alzheimer’s Disease Research Center grant P30AG062421 to BTH, and 1R56AG061196 to BTHAlzheimer’s Association (AACF17-524184 and AACF-17-524184-RAPID to AS-P
Programmed cell senescence during mammalian embryonic development
Cellular senescence disables proliferation in damaged cells, and it is relevant for cancer and aging. Here, we show that senescence occurs during mammalian embryonic development at multiple locations, including the mesonephros and the endolymphatic sac of the inner ear, which we have analyzed in detail. Mechanistically, senescence in both structures is strictly dependent on p21, but independent of DNA damage, p53, or other cell-cycle inhibitors, and it is regulated by the TGF-beta/SMAD and PI3K/FOXO pathways. Developmentally programmed senescence is followed by macrophage infiltration, clearance of senescent cells, and tissue remodeling. Loss of senescence due to the absence of p21 is partially compensated by apoptosis but still results in detectable developmental abnormalities. Importantly, the mesonephros and endolymphatic sac of human embryos also show evidence of senescence. We conclude that the role of developmentally programmed senescence is to promote tissue remodeling and propose that this is the evolutionary origin of damage-induced senescence
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