9 research outputs found
ENHANCED APOPTOSIS IN MCF-7 HUMAN BREAST CANCER CELLS BY BIOGENIC GOLD NANOPARTICLES SYNTHESIZED FROM ARGEMONE MEXICANA LEAF EXTRACT
Objective: To assess the in vitro anticancer activity of A. mexicana mediated gold nanoparticles on MCF-7 breast cancer cells.
Methods: The present study reveals the synthesis and characterization of gold nanoparticles from aqueous extract of Argemone mexicana L. by biological method. UV-Vis spectroscopy, XRD, FT-IR, SEM and TEM analyses revealed that green synthesized gold nanoparticles were 26 ± 5 nm in size and spherical in shape. MCF- 7 Breast cancer cells were treated with different concentrations of green synthesised gold nanoparticles at different time intervals. Cytotoxicity activity and the mechanism of apoptosis were determined by morphological and chromatin cleavage assays.
Results: Green synthesised gold nanoparticles showed dose-dependent cytotoxic and apoptotic effect in MCF-7 breast cancer cells at concentration of 100 ĂŽÂĽg/mL.
Conclusion: The present investigation showed that Argemone mediated gold nanoparticles may be an alternative chemotherapeutic agent for treatment of breast cancer
Mixture of betel leaf, areca nut and tobacco chewing is a risk factor for cytogenetic damage in construction workers from south India
Aim: To determine the cytogenetic effect of betel leaf, areca nut and
tobacco mixture usage among female construction workers in Tamilnadu,
Southern India. Methods: Totally 236 buccal cells and blood samples
were collected from 80 betel quid users and 76 users with tobacco
snuffing habit which were compared with 80 healthy subjects. Peripheral
blood leukocyte cultures were analyzed for chromosomal aberrations (CA)
and exfoliated cells from the buccal mucosa were examined for
micronucleus (MN). Results: Statistically significant (p<0.01)
increase in CA and MN were observed in users with snuffing habit when
compared to users without snuffing habit and controls as confirmed by
chi-square test. Therefore, specific biomarkers on cytogenetic
endpoints might help in planning precautionary measures to reduce oral
cancer risks. Conclusions: The present study can be concluded that a
mixture of betel quid, areca nut and tobacco chewing/snuffing is unsafe
for oral health. The genotoxic effect of smokeless tobacco should be
considered in addition to other known hazards for assessing health
risks
Mixture of betel leaf, areca nut and tobacco chewing is a risk factor for cytogenetic damage in construction workers from south India
determine the cytogenetic effect of betel leaf, areca nut and tobacco mixture usage among female construction workers in Tamilnadu, Southern India. Methods: Totally 236 buccal cells and blood samples were collected from 80 betel quid users and 76 users with tobacco snuffing habit which were compared with 80 healthy subjects. Peripheral blood leukocyte cultures were analyzed for chromosomal aberrations (CA) and exfoliated cells from the buccal mucosa were examined for micronucleus (MN). Results: Statistically significant (p<0.01) increase in CA and MN were observed in users with snuffing habit when compared to users without snuffing habit and controls as confirmed by chi-square test. Therefore, specific biomarkers on cytogenetic endpoints might help in planning precautionary measures to reduce oral cancer risks. Conclusions: The present study can be concluded that a mixture of betel quid, areca nut and tobacco chewing/snuffing is unsafe for oral health. The genotoxic effect of smokeless tobacco should be considered in addition to other known hazards for assessing health risks