A Near-Surface Microstructure Sensor System Used During TOGA COARE. Part II: Turbulence Measurements

New techniques developed for near-surface turbulence measurements during the Tropical Ocean Global Atmosphere (TOGA) Coupled Ocean–Atmosphere Response Experiment (COARE) employ a difference in spatial scales of turbulence and surface waves. According to this approach, high relative speed of the measurements provides separation of the turbulence and surface wave signals. During the TOGA COARE field studies, highresolution probes of pressure, temperature, conductivity, fluctuation velocity, and acceleration were mounted on the bow of the vessel at a 1.7-m depth in an undisturbed region ahead of the moving vessel. The localization in narrow frequency bands of the vibrations of the bow sensors allows accurate calculation of the dissipation rate. A coherent noise reduction algorithm effectively removes vibration contamination of the velocity dataset. Due to the presence of surface waves and the associated pitching of the vessel, the bow probes ‘‘scanned’’ the near-surface layer of the ocean. Contour plots calculated using the bow signals provide a spatial context for the analysis of near-surface turbulence. A fast-moving free-rising profiler equipped by similar probes sampled the near-surface turbulence during stations. Theory of the three-component electromagnetic velocity sensor and examples of data obtained by bow sensors and free-rising profiler are also presented in this paper

Expression of adhesion molecules and cytokines after coronary artery bypass grafting during normothermic and hypothermic cardiac arrest

Objective: Cardiac surgery with cardiopulmonary bypass (CPB) results in vascular injury and tissue damage which involves leukocyte-endothelial interactions mediated by cytokines and adhesion molecules. This study was designed to demonstrate the effect of normothermic and hypothermic CPB to cytokine and soluble adhesion molecule levels in adults and to determine whether these levels correlate to the patients postoperative course. Design and patients: In 25 patients after normothermic and in 25 patients after hypothermic coronary artery bypass grafting with cardiopulmonary bypass (CPB), blood samples for cytokine and soluble adhesion molecule analysis were taken preoperatively, 24, 36, 48 h, and 6 days postoperatively. Soluble adhesion molecules (sE-selectin, sICAM-1) were measured by ELISA and cytokines (TNF-α, IL-6, IL-8) by chemilumenscent-immunoassay. Clinical data were collected prospectively. Results: Postoperatively, adhesion molecule and cytokine levels were significantly elevated after CPB. Mean plasma levels of sICAM-1 was 2.4-fold higher after 6 days. Mean plasma concentration of sE-selectin peaked after 48 h with a 2-fold increase compared to normothermic conditions. In the hypothermia group sICAM-1, sE-selectin, IL-6, and IL-8 showed significantly higher levels (P≪0.0057, P≪0.0012, P≪0.0419, P≪0.0145) after 24 h compared to the normothermia group. No clinical differences were seen. Conclusion: Adhesion molecules and cytokines are elevated after CPB. Patients after hypothermic CPB show significant higher sICAM-1, sE-selectin, IL-6, and IL-8 levels after 24 h compared to normothermic conditions. These results are mainly due to longer CPB and crossclamp times but do not alter the patient's postoperative cours

Seeing like the international community: how peacebuilding failed (and survived) in Tajikistan

Post-print version. 18 month embargo by the publisher. Article will be released April 2010.The international community claims transformative power over post-conflict spaces via the concept of peacebuilding. International actors discursively make space for themselves in settings such as the Central Asian state of Tajikistan which endured a civil war during the 1990s and has only seen an end to widespread political violence in recent years. With the work of James C. Scott, this paper challenges the notion that post-conflict spaces are merely the objects of international intervention. It reveals how, even in cases of apparent stability such as that of Tajikistan, international actors fail to achieve their ostensible goals for that place yet make space for themselves in that place. International peacebuilders may provide essential resources for the re-emergence of local forms of order yet these symbolic and material resources are inevitably re-interpreted and re-appropriated by local actors to serve purposes which may be the opposite of their aims. However, despite this ‘failure’ of peacebuilding it nevertheless survives as a discursive construction through highly subjective processes of monitoring and evaluation. So maintained, peacebuilding is a constitutive element of world order where the necessity of intervention for humanitarian, democratic and statebuilding ends goes unchallenged. This raises the question of what or where – in spatial terms – is the locus of international intervention: the local recipients of peacebuilding programmes (who are the ostensible targets) or ‘the International Community’ itself (whose space is re-inscribed as that of an imperfect but necessary regulator of world order)

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

© 2024 The Authors. Journal of Extracellular Vesicles, published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY), https://creativecommons.org/licenses/by/4.0/Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.Peer reviewe

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly