117 research outputs found
Association of an ice-nucleating pseudomonad with cultures of the marine dinoflagellate, Heterocapsa niei
The observations that terrestrial ice nuclei can have a biogenic origin and that certain bacteria can initiate freezing at exceptionally warm temperatures (−1.5°C) are now well documented. Less well understood are active ice nuclei (−2 to −5°C) found in sea water, marine fog and the marine atmosphere in general. Recently, the authors have isolated an ice nucleation-active (INA) bacterial strain (FB 1032) from cultures of the marine dinoflagellate, Heterocapsa niei. FB 1032 is halotolerant and phenotypically similar to Pseudomonas fluorescens biotype G, although it differs from biotype G strains in its bacteriophage sensitivity and expression of the INA phenotype. A search for the INA pseudomonad in sea water at La Jolla, California was unsuccessful, but several INA Erwinia sp. were isolated. The possible terrestrial origin of these INA bacteria is suggested
Reemerging Rabies and Lack of Systemic Surveillance in People’s Republic of China
Standardized protocols and diagnostic-based surveillance are imperative for detection and elimination
Large Observatory for X-ray Timing (LOFT-P): A Probe-Class Mission Concept Study
LOFT-P is a mission concept for a NASA Astrophysics Probe-Class (less than 500M and $1B. We submitted a white paper4 in response to NASA PhysPAG's call for white papers: Probe-class Mission Concepts, describing LOFT-P science and a simple extrapolation from the ESA study costs. The next step for probe-class missions will be input into the NASA Astrophysics Decadal Survey to encourage the creation of a probe-class opportunity. We report on a 2016 study by MSFC's Advanced Concepts Office of LOFT-P, a US-led probe-class LOFT concept
Status Report of the DPHEP Study Group: Towards a Global Effort for Sustainable Data Preservation in High Energy Physics
Data from high-energy physics (HEP) experiments are collected with
significant financial and human effort and are mostly unique. An
inter-experimental study group on HEP data preservation and long-term analysis
was convened as a panel of the International Committee for Future Accelerators
(ICFA). The group was formed by large collider-based experiments and
investigated the technical and organisational aspects of HEP data preservation.
An intermediate report was released in November 2009 addressing the general
issues of data preservation in HEP. This paper includes and extends the
intermediate report. It provides an analysis of the research case for data
preservation and a detailed description of the various projects at experiment,
laboratory and international levels. In addition, the paper provides a concrete
proposal for an international organisation in charge of the data management and
policies in high-energy physics
Large Observatory for x-ray Timing (LOFT-P): a Probe-class mission concept study
LOFT-P is a concept for a NASA Astrophysics Probe-Class (<$1B) X-ray timing
mission, based on the LOFT concept originally proposed to ESAs M3 and M4 calls.
LOFT-P requires very large collecting area (>6 m^2, >10x RXTE), high time
resolution, good spectral resolution, broad-band spectral coverage (2-30 keV),
highly flexible scheduling, and an ability to detect and respond promptly to
time-critical targets of opportunity. It addresses science questions such as:
What is the equation of state of ultra dense matter? What are the effects of
strong gravity on matter spiraling into black holes? It would be optimized for
sub-millisecond timing to study phenomena at the natural timescales of neutron
star surfaces and black hole event horizons and to measure mass and spin of
black holes. These measurements are synergistic to imaging and high-resolution
spectroscopy instruments, addressing much smaller distance scales than are
possible without very long baseline X-ray interferometry, and using
complementary techniques to address the geometry and dynamics of emission
regions. A sky monitor (2-50 keV) acts as a trigger for pointed observations,
providing high duty cycle, high time resolution monitoring of the X-ray sky
with ~20 times the sensitivity of the RXTE All-Sky Monitor, enabling
multi-wavelength and multi-messenger studies. A probe-class mission concept
would employ lightweight collimator technology and large-area solid-state
detectors, technologies which have been recently greatly advanced during the
ESA M3 study. Given the large community interested in LOFT (>800 supporters,
the scientific productivity of this mission is expected to be very high,
similar to or greater than RXTE (~2000 refereed publications). We describe the
results of a study, recently completed by the MSFC Advanced Concepts Office,
that demonstrates that LOFT-P is feasible within a NASA probe-class mission
budget.Comment: Proc. SPIE 9905, Space Telescopes and Instrumentation 2016:
Ultraviolet to Gamma Ray, 99054Y (July 18, 2016
The Buffer Gas Beam: An Intense, Cold, and Slow Source for Atoms and Molecules
Beams of atoms and molecules are stalwart tools for spectroscopy and studies
of collisional processes. The supersonic expansion technique can create cold
beams of many species of atoms and molecules. However, the resulting beam is
typically moving at a speed of 300-600 m/s in the lab frame, and for a large
class of species has insufficient flux (i.e. brightness) for important
applications. In contrast, buffer gas beams can be a superior method in many
cases, producing cold and relatively slow molecules in the lab frame with high
brightness and great versatility. There are basic differences between
supersonic and buffer gas cooled beams regarding particular technological
advantages and constraints. At present, it is clear that not all of the
possible variations on the buffer gas method have been studied. In this review,
we will present a survey of the current state of the art in buffer gas beams,
and explore some of the possible future directions that these new methods might
take
Hepatitis C virus envelope glycoprotein fitness defines virus population composition following transmission to a new host
Genetic variability is a hallmark of RNA virus populations. However, transmission to a new host often results in a marked decrease in population diversity. This genetic bottlenecking is observed during hepatitis C virus (HCV) transmission and can arise via a selective sweep or through the founder effect. To model HCV transmission, we utilized chimeric SCID/Alb-uPA mice with transplanted human hepatocytes and infected them with a human serum HCV inoculum. E1E2 glycoprotein gene sequences in the donor inoculum and recipient mice were determined following single-genome amplification (SGA). In independent experiments, using mice with liver cells grafted from different sources, an E1E2 variant undetectable in the source inoculum was selected for during transmission. Bayesian coalescent analyses indicated that this variant arose in the inoculum pretransmission. Transmitted variants that established initial infection harbored key substitutions in E1E2 outside HVR1. Notably, all posttransmission E1E2s had lost a potential N-linked glycosylation site (PNGS) in E2. In lentiviral pseudoparticle assays, the major posttransmission E1E2 variant conferred an increased capacity for entry compared to the major variant present in the inoculum. Together, these data demonstrate that increased envelope glycoprotein fitness can drive selective outgrowth of minor variants posttransmission and that loss of a PNGS is integral to this improved phenotype. Mathematical modeling of the dynamics of competing HCV variants indicated that relatively modest differences in glycoprotein fitness can result in marked shifts in virus population composition. Overall, these data provide important insights into the dynamics and selection of HCV populations during transmission
Expression of the retinoic acid catabolic enzyme CYP26B1 in the human brain to maintain signaling homeostasis
Date of Acceptance: 27/08/2015 Funding was provided by the Wellcome Trust grant WT081633MA-NCE and Biological Sciences Research Council Grant BB/K001043/1. Prof Fragoso is the recipient of a Post Doctoral Science without Borders grant from the Brazilian National Council for Scientific and Technological Development (CNPq, 237450/2012-7).Peer reviewedPublisher PD
Biomass production of herbaceous energy crops in the United States: field trial results and yield potential maps from the multiyear regional feedstock partnership
Current knowledge of yield potential and best agronomic management practices for perennial bioenergy grasses is primarily derived from small-scale and short-term studies, yet these studies inform policy at the national scale. In an effort to learn more about how bioenergy grasses perform across multiple locations and years, the U.S. Department of Energy (US DOE)/Sun Grant Initiative Regional Feedstock Partnership was initiated in 2008. The objectives of the Feedstock Partnership were to (1) provide a wide range of information for feedstock selection (species choice) and management practice options for a variety of regions and (2) develop national maps of potential feedstock yield for each of the herbaceous species evaluated. The Feedstock Partnership expands our previous understanding of the bioenergy potential of switchgrass, Miscanthus, sorghum, energycane, and prairie mixtures on Conservation Reserve Program land by conducting long-term, replicated trials of each species at diverse environments in the U.S. Trials were initiated between 2008 and 2010 and completed between 2012 and 2015 depending on species. Field-scale plots were utilized for switchgrass and Conservation Reserve Program trials to use traditional agricultural machinery. This is important as we know that the smaller scale studies often overestimated yield potential of some of these species. Insufficient vegetative propagules of energycane and Miscanthus prohibited farm-scale trials of these species. The Feedstock Partnership studies also confirmed that environmental differences across years and across sites had a large impact on biomass production. Nitrogen application had variable effects across feedstocks, but some nitrogen fertilizer generally had a positive effect. National yield potential maps were developed using PRISM-ELM for each species in the Feedstock Partnership. This manuscript, with the accompanying supplemental data, will be useful in making decisions about feedstock selection as well as agronomic practices across a wide region of the country
- …