Hydrothermal Routes for the Synthesis of CdSe Core Quantum Dots

23 pagesThe synthesis of QDs in aqueous solution is still pursued in hopes of providing a material that is easily fabricated and functionalized. During many years, the synthesis of CdSe in aqueous media has been investigated with limited success due to the low quantum yields and poor crystallinity of the nanoparticles produced. Moreover, the fluorescence of the CdSe QDs obtained by this approach cannot be controlled over a wide range. Direct synthesis of CdSe QDs in water is however a promising alternative route to organometallic reactions and facilitates the use of the dots in biological systems. Hydrothermal synthesis offers also the following advantages: (1) lower reaction temperatures with comparable PL QY; (2) does not use toxic and expensive organometallic reagents; (3) surface functionalization during synthesis without further treatment; (4) comparatively smaller sizes (3-8 nm) than those obtained after encapsulation of hydrophobic QDs with amphiphilic lipids or polymers (generally > 20 nm); (5) more reproducible. Thus, it is still a challenging task to develop a method for preparing CdSe QDs with the desired quality under mild and environmentally friendly conditions using a low-cost and simple method. In this review, we summarize the aqueous solution-based syntheses of CdSe nanocrystals developed in recent years and the applications of these nanocrystals

Kriterien zur Stickstoffeffizienz moderner Winterweizensorten und deren Eignung für low-input Anbausysteme

Nitrogen deficiency is one of the most important reasons for reduced quality and yield stability in wheat production under low-input conditions. The present project intends to develop criteria allowing to find varieties that are adapted for low-input agricultural conditions. Therefore, yield and nitrogen uptake and use dynamics of eleven modern wheat varieties have been studied under low and high input conditions on a long term field experiment in Changins. This preliminary report shows that nitrogen efficiency defined as yield per available nitrogen is a useful criterion to distinguish nutrient efficiency under high nitrogen conditons but not when nitrogen is lacking. In further studies we will focus on backing quality as a potential indicator for nitrogen efficiency at low nitrogen conditions

Expected Performance of a Self-Coherent Camera

Residual wavefront errors in optical elements limit the performance of coronagraphs. To improve their efficiency, different types of devices have been proposed to correct or calibrate these errors. In this paper, we study one of these techniques proposed by Baudoz et al. 2006 and called Self-Coherent Camera (SCC). The principle of this instrument is based on the lack of coherence between the stellar light and the planet that is searched for. After recalling the principle of the SCC, we simulate its performance under realistic conditions and compare it with the performance of differential imaging.Comment: 6 pages, 4 figure

Dating and morpho-stratigraphy of uplifted marine terraces in the Makran subduction zone (Iran)

The western part of the Makran subduction zone (Iran) is currently experiencing active surface uplift, as attested by the presence of emerged marine terraces along the coast. To better understand the uplift recorded by these terraces, we investigated seven localities along the Iranian Makran and we performed radiocarbon, 230Th∕U and optically stimulated luminescence (OSL) dating of the layers of marine sediments deposited on top of the terraces. This enabled us to correlate the terraces regionally and to assign them to different Quaternary sea-level highstands. Our results show east–west variations in surface uplift rates mostly between 0.05 and 1.2 mm yr−1. We detected a region of anomalously high uplift rate, where two MIS 3 terraces are emerged, but we are uncertain how to interpret these results in a geologically coherent context. Although it is presently not clear whether the uplift of the terraces is linked to the occurrence of large megathrust earthquakes, our results highlight rapid surface uplift for a subduction zone context and heterogeneous accumulation of deformation in the overriding plate

Performance of eleven winter wheat varieties in a long term experiment on mineral nitrogen and organic fertilisation

There is an increasing awareness about the need for improving nitrogen use efficiency in crop production in order to meet economic and ecological standards. The present work provides insight into the varietal factors of wheat that determine nitrogen use in the plant. The performance of eleven winter wheat varieties in terms of grain yield and nitrogen uptake and use efficiency was tested within the framework of a 40-year long term field experiment of organic enrichment and mineral nitrogen fertilisation treatments. Globally, organic enrichment had a beneficial effect on the yield and grain nitrogen concentration and showed a strong interaction with the amount of applied mineral nitrogen fertiliser. Manure application generally had positive effects on varietal performances mainly due to indirect longterm effects on the soil properties. Varieties showed a broad range of responses to the treatments of the long term experiment, revealing significant genotype × environment interactions. Nevertheless, the varieties which performed well at high input levels were also the best at low input levels, suggesting that the genotype × environment interactions were not strong enough to inverse the performance ranking.Similarly, the varietal traits associated with high yielding or grain nitrogen concentration in high input conditions were the same as those identified under low input conditions. To conclude, these results suggest that the selection of wheat for nitrogen efficiency is possible under any nitrogen fertilisation regime.However, to be adapted to low input or organic agriculture, varieties also need traits other than nutrientuse efficiency, for example, disease resistance, resilience to abiotic stresses and competitiveness against weeds

Microfluidic characterisation reveals broad range of SARS-CoV-2 antibody affinity in human plasma.

Funder: Herchel Smith FundFunder: St John’s College CambridgeFunder: Centre for Misfolding Diseases, CambridgeFunder: Swiss FCS and the Forschungskredit of the University of ZurichFunder: Frances and Augustus Newman FoundationFunder: BBRSCFunder: NOMIS FoundationThe clinical outcome of SARS-CoV-2 infections, which can range from asymptomatic to lethal, is crucially shaped by the concentration of antiviral antibodies and by their affinity to their targets. However, the affinity of polyclonal antibody responses in plasma is difficult to measure. Here we used microfluidic antibody affinity profiling (MAAP) to determine the aggregate affinities and concentrations of anti-SARS-CoV-2 antibodies in plasma samples of 42 seropositive individuals, 19 of which were healthy donors, 20 displayed mild symptoms, and 3 were critically ill. We found that dissociation constants, K d, of anti-receptor-binding domain antibodies spanned 2.5 orders of magnitude from sub-nanomolar to 43 nM. Using MAAP we found that antibodies of seropositive individuals induced the dissociation of pre-formed spike-ACE2 receptor complexes, which indicates that MAAP can be adapted as a complementary receptor competition assay. By comparison with cytopathic effect-based neutralisation assays, we show that MAAP can reliably predict the cellular neutralisation ability of sera, which may be an important consideration when selecting the most effective samples for therapeutic plasmapheresis and tracking the success of vaccinations

Continuous population-level monitoring of SARS-CoV-2 seroprevalence in a large European metropolitan region

Effective public health measures against SARS-CoV-2 require granular knowledge of population-level immune responses. We developed a Tripartite Automated Blood Immunoassay (TRABI) to assess the IgG response against three SARS-CoV-2 proteins. We used TRABI for continuous seromonitoring of hospital patients and blood donors (n = 72'250) in the canton of Zurich from December 2019 to December 2020 (pre-vaccine period). We found that antibodies waned with a half-life of 75 days, whereas the cumulative incidence rose from 2.3% in June 2020 to 12.2% in mid-December 2020. A follow-up health survey indicated that about 10% of patients infected with wildtype SARS-CoV-2 sustained some symptoms at least twelve months post COVID-19. Crucially, we found no evidence of a difference in long-term complications between those whose infection was symptomatic and those with asymptomatic acute infection. The cohort of asymptomatic SARS-CoV-2-infected subjects represents a resource for the study of chronic and possibly unexpected sequelae

Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis

The early development of many animals relies on the posttranscriptional regulations of maternally stored mRNAs. In particular, the translation of maternal mRNAs is tightly controlled during oocyte maturation and early mitotic cycles in Xenopus. The Embryonic Deadenylation ElemeNt (EDEN) and its associated protein EDEN-BP are known to trigger deadenylation and translational silencing to several mRNAs bearing an EDEN. This Xenopus RNA-binding protein is an ortholog of the human protein CUG-BP1/CELF1. Five mRNAs, encoding cell cycle regulators and a protein involved in the notch pathway, have been identified as being deadenylated by EDEN/EDEN-BP. To identify new EDEN-BP targets, we immunoprecipitated EDEN-BP/mRNA complexes from Xenopus tropicalis egg extracts. We identified 153 mRNAs as new binding targets for EDEN-BP using microarrays. Sequence analyses of the 3′ untranslated regions of the newly identified EDEN-BP targets reveal an enrichment in putative EDEN sequences. EDEN-BP binding to a subset of the targets was confirmed both in vitro and in vivo. Among the newly identified targets, Cdk1, a key player of oocyte maturation and cell cycle progression, is specifically targeted by its 3′ UTR for an EDEN-BP-dependent deadenylation after fertilization