172 research outputs found
Teacher collaboration : why isn\u27t it working?
Professional learning communities have started to develop in schools as an approach to look closely at student needs and implement changes in instructional practices. When teachers come together in professional learning communities they are ultimately collaborating. Through collaborative sessions teachers determine strengths and weaknesses of students and then work together to decide instructional strategies that will potentially lead to an increase in student achievement. The focus of this paper is to examine the benefits and barriers of successful teacher collaboration and also suggest an action plan to address barriers of collaboration for a team of 1st grade teachers
Strong-coupling expansions for chiral models of electroweak symmetry breaking
We consider chiral models with fermions in the limit of
infinitely large local bare Yukawa coupling. When the scalar field is subject
to non-linear constraint, phase transitions in these models are seen to be
identical to those in the corresponding purely bosonic ones. Relaxing the
non-linear constraint, we compute the seventh-order strong-coupling series for
the susceptibility in these models and analyze them numerically for the
case. We find that in four dimensions the approach to the
phase transition follows to a good accuracy the mean-field critical behavior,
indicating the absence of non-trivial fixed points at strong coupling and being
consistent with the first-order nature of the transition. In three dimensions,
the strongly-coupled bosonic model (without gauge fields) has
a first-order transition strong enough to accommodate electroweak baryogenesis
only for a narrow region of the bare parameter space.Comment: 11 pages, latex, no figure
Search for flavor-changing neutral currents and lepton-family-number violation in two-body D0 decays
Results of a search for the three neutral charm decays, D0 -> mu e, D0 -> mu
mu, and D0 -> e e, are presented. This study was based on data collected in
Experiment 789 at the Fermi National Accelerator Laboratory using 800 GeV/c
proton-Au and proton-Be interactions. No evidence is found for any of the
decays. Upper limits on the branching ratios, at the 90% confidence level, are
obtained.Comment: 28 pages, 18 figures. Submitted to Physical Review
A Glutamic Acid-Rich Protein Identified in Verticillium dahliae from an Insertional Mutagenesis Affects Microsclerotial Formation and Pathogenicity
Verticillium dahliae Kleb. is a phytopathogenic fungus that causes wilt disease in a wide range of crops, including cotton. The life cycle of V. dahliae includes three vegetative phases: parasitic, saprophytic and dormant. The dormant microsclerotia are the primary infectious propagules, which germinate when they are stimulated by root exudates. In this study, we report the first application of Agrobacterium tumefaciens-mediated transformation (ATMT) for construction of insertional mutants from a virulent defoliating isolate of V. dahliae (V592). Changes in morphology, especially a lack of melanized microsclerotia or pigmentation traits, were observed in mutants. Together with the established laboratory unimpaired root dip-inoculation approach, we found insertional mutants to be affected in their pathogenicities in cotton. One of the genes tagged in a pathogenicity mutant encoded a glutamic acid-rich protein (VdGARP1), which shared no significant similarity to any known annotated gene. The vdgarp1 mutant showed vigorous mycelium growth with a significant delay in melanized microsclerotial formation. The expression of VdGARP1 in the wild type V529 was organ-specific and differentially regulated by different stress agencies and conditions, in addition to being stimulated by cotton root extract in liquid culture medium. Under extreme infertile nutrient conditions, VdGARP1 was not necessary for melanized microsclerotial formation. Taken together, our data suggest that VdGARP1 plays an important role in sensing infertile nutrient conditions in infected cells to promote a transfer from saprophytic to dormant microsclerotia for long-term survival. Overall, our findings indicate that insertional mutagenesis by ATMT is a valuable tool for the genome-wide analysis of gene function and identification of pathogenicity genes in this important cotton pathogen
Desirable traits of a good biocontrol agent against Verticillium wilt
The soil-borne fungus Verticillium causes serious vascular disease in a wide variety of annual crops and woody perennials. Verticillium wilt is notoriously difficult to control by conventional methods, so there is great potential for biocontrol to manage this disease. In this study we aimed to review the research about Verticillium biocontrol to get a better understanding of characteristics that are desirable in a biocontrol agent (BCA) against Verticillium wilt. We only considered studies in which the BCAs were tested on plants. Most biocontrol studies were focused on plants of the Solanaceae, Malvaceae, and Brassicaceae and within these families eggplant, cotton, and oilseed rape were the most studied crops. The list of bacterial BCAs with potential against Verticillium was dominated by endophytic Bacillus and Pseudomonas isolates, while non-pathogenic xylem-colonizing Verticillium and Fusarium isolates topped the fungal list. Predominant modes of action involved in biocontrol were inhibition of primary inoculum germination, plant growth promotion, competition and induced resistance. Many BCAs showed in vitro antibiosis and mycoparasitism but these traits were not correlated with activity in vivo and there is no evidence that they play a role in planta. Good BCAs were obtained from soils suppressive to Verticillium wilt, disease suppressive composts, and healthy plants in infested fields. Desirable characteristics in a BCA against Verticillium are the ability to (1) affect the survival or germination of microsclerotia, (2) colonize the xylem and/or cortex and compete with the pathogen for nutrients and/or space, (3) induce resistance responses in the plant and/or (4) promote plant growth. Potential BCAs should be screened in conditions that resemble the field situation to increase the chance of successful use in practice. Furthermore, issues such as large scale production, formulation, preservation conditions, shelf life, and application methods should be considered early in the process of selecting BCAs against Verticillium
Silencing of Vlaro2 for chorismate synthase revealed that the phytopathogen Verticillium longisporum induces the cross-pathway control in the xylem
The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum (VL) has been generated. VL enters its host Brassica napus through the roots and colonizes the xylem vessels. The xylem contains little nutrients including low concentrations of amino acids. We isolated the gene Vlaro2 encoding chorismate synthase by complementation of the corresponding yeast mutant strain. Chorismate synthase produces the first branch point intermediate of aromatic amino acid biosynthesis. A novel RNA-mediated gene silencing method reduced gene expression of both isogenes by 80% and resulted in a bradytrophic mutant, which is a leaky auxotroph due to impaired expression of chorismate synthase. In contrast to the wild type, silencing resulted in increased expression of the cross-pathway regulatory gene VlcpcA (similar to cpcA/GCN4) during saprotrophic life. The mutant fungus is still able to infect the host plant B. napus and the model Arabidopsis thaliana with reduced efficiency. VlcpcA expression is increased in planta in the mutant and the wild-type fungus. We assume that xylem colonization requires induction of the cross-pathway control, presumably because the fungus has to overcome imbalanced amino acid supply in the xylem
Identification of Cauliflower Cultivars that Differ in Susceptibility to Verticillium longisporum using Different Inoculation Methods
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Preliminary results from Fermilab E789
Fermilab experiment 789 studies low-multiplicity decays of neutral D and B mesons in a high-rate fixed-target environment. Preliminary results from the 1991 run are presented
Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen
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