Safety of improved Milbond-TX mycotoxin binder when fed to broiler breeders above recommended levels

An increasing concern in poultry nutrition is the effects of mycotoxins in contaminated grain. Several new products have come onto the market that chemically bind these toxins preventing mycotoxicosis. However, many of these products have not been tested for safety if accidently overfed to broiler breeders. In order to simulate a feed mixing error at a feed mill, Improved Milbond-TX® was overfed to broiler breeders to see if this would cause any negative effects on bird performance. A typical corn-soybean based diet supplemented with Milbond-TX mycotoxin binder at three different levels of inclusion (0%, 0.5%, and 1%) was fed to 300 broiler breeder hens. Data were collected on egg production, egg weights, hatchability, fertility, and chick weights from 24 to 35 weeks of age. Eggs per hen housed were not significantly different between the three treatments. The differences in egg weights, hatchability, fertility, and chick weights were also insignificant among the three treatments. We were able to conclude that overfeeding Improved Milbond-TX had no negative effect on bird performance and is safe to feed at a level of up to 1%

Familienrekonstitution avant la lettre: Volksgenealogie und historische Demographie im Kontext von völkischer Wissenschaft, Rassenkunde und Rassenpolitik

International literature on the history of historical demography leaves no doubt that Louis Henry and his associates ‘invented’ the method of family reconstitution in the 1950s and revolutionized the study of demographic behaviour in the past by using individual level data from parish registers. This article adds a largely ignored prehistory to this narrative through three points. First, from the 1920s, family reconstitutions were carried out on a large scale in German-speaking countries. They served a wide variety of scholarly, popular and political purposes. Second, many family reconstitutions were used for analysing demographic behaviour, often in ways similar to Henry’s historical demography. Third, most family reconstitutions and a large part of demographic research based on them were closely linked to racial science. Many of these scholarly activities helped propagate and implement the racial policy of the Nazi regime, and many of the institutions and persons promoting them were strongly involved in enforcing it.International literature on the history of historical demography leaves no doubt that Louis Henry and his associates ‘invented’ the method of family reconstitution in the 1950s and revolutionized the study of demographic behaviour in the past by using individual level data from parish registers. This article adds a largely ignored prehistory to this narrative through three points. First, from the 1920s, family reconstitutions were carried out on a large scale in German-speaking countries. They served a wide variety of scholarly, popular and political purposes. Second, many family reconstitutions were used for analysing demographic behaviour, often in ways similar to Henry’s historical demography. Third, most family reconstitutions and a large part of demographic research based on them were closely linked to racial science. Many of these scholarly activities helped propagate and implement the racial policy of the Nazi regime, and many of the institutions and persons promoting them were strongly involved in enforcing it

Predicting non-response in patient-reported outcome measures: results from the Swiss quality assurance programme in cardiac inpatient rehabilitation

Background Quality assurance programmes measure and compare certain health outcomes to ensure high quality care in the health care sector. The outcome health related quality of life (HRQOL) is typically measured by patient-reported outcome measures (PROMs). However, certain patient groups are less likely to respond to PROMs than others. This non-response bias can potentially distort results in quality assurance programmes. Our study aims to identify relevant predictors for non-response during assessment using the PROM MacNew Heart Disease questionnaire in cardiac rehabilitation. Methods This is a cross-sectional study based on data from the Swiss external quality assurance programme. All patients aged 18 years or older who underwent inpatient cardiac rehabilitation in 16 Swiss rehabilitation clinics between 2016 and 2019 were included. Patients’ sociodemographic and basic medical data were analysed descriptively by comparing two groups: non-responders and responders. We used a random intercept logistic regression model to estimate associations of patient characteristics and clinic differences with non-response. Results Of 24 572 patients, there were 33.3% non-responders and 66.7% responders. The mean age was 70; 31.0% were women. The regression model showed that being female was associated with non-response (odds ratio (OR) 1.22; 95% confidence interval (95% CI) 1.14–1.30), as well as having no supplementary health insurance (OR 1.49; 95% CI 1.39–1.59). Each additional year of age increased the chance of non-response by an OR of 1.02 (95% CI 1.02–1.02). Not being a first language speaker of German, French, or Italian increased the chance of non-response by an OR of 6.94 (95% CI 6.03–7.99). Patients admitted directly from acute care had a higher chance of non-response (OR 1.23; 95% CI 1.10–1.38), as well as patients being discharged back into acute care after rehabilitation (OR 3.89; 95% CI 3.00–5.04). Each point on the cumulative illness rating scale (CIRS) total score increased the chance of non-response by an OR of 1.05 (95% CI 1.04–1.05). Certain diagnoses also influenced the chance of non-response. Even after adjustment for known confounders, response rates differed substantially between the 16 clinics. Conclusion We have found significant non-response bias among certain patient groups, as well as across different treatment facilities. Measures to improve response rates among patients with known barriers to participation, as well as among different treatment facilities need to be considered, particularly when PROMs are being used for comparison of providers in quality assurance programmes or outcome evaluation

Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate

Contains fulltext : 34911.pdf (publisher's version ) (Open Access)BACKGROUND: The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited. RESULTS: Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of the marmoset microarray with labelled RNA from hippocampus, cortex and a panel of 7 different peripheral tissues resulted in high detection rates of 85% in the neuronal tissues and on average 70% in the non-neuronal tissues. The expression profiles of the 2 neuronal tissues, hippocampus and cortex, were highly similar, as indicated by a correlation coefficient of 0.96. Several transcripts with a tissue-specific pattern of expression were identified. Besides the marmoset microarray we have generated 3215 ESTs derived from marmoset hippocampus, which have been annotated and submitted to GenBank [GenBank: EF214838-EF215447, EH380242-EH382846]. CONCLUSION: We have generated the first marmoset-specific DNA microarray and demonstrated its use to characterise large-scale gene expression profiles of hippocampus but also of other neuronal and non-neuronal tissues. In addition, we have generated a large collection of ESTs of marmoset origin, which are now available in the public domain. These new tools will facilitate molecular genetic research into this non-human primate animal model

Prenatal Programming of Metabolic Syndrome in the Common Marmoset Is Associated With Increased Expression of 11β-Hydroxysteroid Dehydrogenase Type 1

OBJECTIVE: Recent studies in humans and animal models of obesity have shown increased adipose tissue activity of 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1), which amplifies local tissue glucocorticoid concentrations. The reasons for this 11beta-HSD1 dysregulation are unknown. Here, we tested whether 11beta-HSD1 expression, like the metabolic syndrome, is "programmed" by prenatal environmental events in a nonhuman primate model, the common marmoset monkey. RESEARCH DESIGN AND METHODS: We used a "fetal programming" paradigm where brief antenatal exposure to glucocorticoids leads to the metabolic syndrome in the offspring. Pregnant marmosets were given the synthetic glucocorticoid dexamethasone orally for 1 week in either early or late gestation, or they were given vehicle. Tissue 11beta-HSD1 and glucocorticoid receptor mRNA expression were examined in the offspring at 4 and 24 months of age. RESULTS: Prenatal dexamethasone administration, selectively during late gestation, resulted in early and persistent elevations in 11beta-HSD1 mRNA expression and activity in the liver, pancreas, and subcutaneous-but not visceral-fat. The increase in 11beta-HSD1 occurred before animals developed obesity or overt features of the metabolic syndrome. In contrast to rodents, in utero dexamethasone exposure did not alter glucocorticoid receptor expression in metabolic tissues in marmosets. CONCLUSIONS: These data suggest that long-term upregulation of 11beta-HSD1 in metabolically active tissues may follow prenatal "stress" hormone exposure and indicates a novel mechanism for fetal origins of adult obesity and the metabolic syndrome

Comparative Analysis of the Effects of Neurotrophic Factors CDNF and GDNF in a Nonhuman Primate Model of Parkinson's Disease

Cerebral dopamine neurotrophic factor (CDNF) belongs to a newly discovered family of evolutionarily conserved neurotrophic factors. We demonstrate for the first time a therapeutic effect of CDNF in a unilateral 6-hydroxydopamine (6-OHDA) lesion model of Parkinson's disease in marmoset monkeys. Furthermore, we tested the impact of high chronic doses of human recombinant CDNF on unlesionedmonkeys and analyzed the amino acid sequence ofmarmoset CDNF. The severity of 6-OHDA lesions and treatment effects weremonitored in vivo using 123I-FP-CIT (DaTSCAN) SPECT. Quantitative analysis of 123I-FP-CIT SPECT showed a significant increase of dopamine transporter binding activity in lesioned animals treated with CDNF. Glial cell line-derived neurotrophic factor (GDNF), a well-characterized and potent neurotrophic factor for dopamine neurons, served as a control in a parallel comparison with CDNF. By contrast with CDNF, only single animals responded to the treatment with GDNF, but no statistical difference was observed in the GDNF group. However, increased numbers of tyrosine hydroxylase immunoreactive neurons, observed within the lesioned caudate nucleus of GDNF-treated animals, indicate a strong bioactive potential of GDNF.Peer reviewe