The decision-making process for breast reconstruction after cancer surgery: representations of heterosexual couples in longstanding relationships

International audienceOBJECTIVES: Most people deal with intrusive life events such as cancer and the care trajectory together with their intimate partners. To our knowledge, no research has studied the involvement of the partner in the decision-making process regarding breast reconstruction (BR) after cancer. This study aimed to gain a better understanding of the couples' decision-making process for BR in the cancer context and particularly to investigate the partners' involvement in this process.METHOD: Eighteen participants (nine women who underwent a mastectomy following a first breast cancer and their intimate partners) took part in this study. We conducted semidirective interviews, and a general inductive approach was chosen to capture the representations of the couples.RESULTS: The women in the sample were aged between 33 and 66 years (M = 54, SD = 7.5) and their partner between 40 and 76 years (M = 59, SD = 11.6). The duration of their intimate relationship was on average 18 years (SD = 10.4; minimum = 4; maximum = 33). The analysis revealed 11 major themes. The two most salient ones were 'external influence' and 'implication of the partner'. The exploration of the subthemes revealed that the decision-making process is often reported as an interrelated experience by the couples and as a dyadic stressor. The partner's role is depicted as consultative and mostly supportive.CONCLUSION: These results provide new insights on the involvement of the partner in decision-making. Thus, it now seems crucial to develop a prospective study, which will help understand the progression of the decision-making process over time. Statement of contribution What is already known on this subject? Most people deal with intrusive life events such as cancer and the care trajectory together with their intimate partners. Shared decision-making between patients and physicians is now the 'gold standard' in Western Europe and the United States. However, in the context of breast reconstruction (BR) after cancer, factors guiding the decision-making process for BR, especially the potential involvement of the partner, are not very well understood. What does this study add? Provides a qualitative insight on the specific nature of heterosexual couples' representations regarding the decision-making process for breast reconstruction after cancer. Reveals that the decision-making process is often reported as an interrelated experience by the couples and as a dyadic stressor. Underlines the consultative function of partners with women engaged in breast reconstruction

Investigating the effect of available redox protein ratios for the conversion of a steroid by a myxobacterial CYP260A1

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136683/1/feb212619.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136683/2/feb212619_am.pd

Computer-based intrapartum fetal monitoring and beyond: A review of the 2nd Workshop on Signal Processing and Monitoring in Labor (October 2017, Oxford, UK).

The second Signal Processing and Monitoring in Labor workshop gathered researchers who utilize promising new research strategies and initiatives to tackle the challenges of intrapartum fetal monitoring. The workshop included a series of lectures and discussions focusing on: new algorithms and techniques for cardiotocogoraphy (CTG) and electrocardiogram acquisition and analyses; the results of a CTG evaluation challenge comparing state-of-the-art computerized methods and visual interpretation for the detection of arterial cord pH <7.05 at birth; the lack of consensus about the role of intrapartum acidemia in the etiology of fetal brain injury; the differences between methods for CTG analysis "mimicking" expert clinicians and those derived from "data-driven" analyses; a critical review of the results from two randomized controlled trials testing the former in clinical practice; and relevant insights from modern physiology-based studies. We concluded that the automated algorithms performed comparably to each other and to clinical assessment of the CTG. However, the sensitivity and specificity urgently need to be improved (both computerized and visual assessment). Data-driven CTG evaluation requires further work with large multicenter datasets based on well-defined labor outcomes. And before first tests in the clinic, there are important lessons to be learnt from clinical trials that tested automated algorithms mimicking expert CTG interpretation. In addition, transabdominal fetal electrocardiogram monitoring provides reliable CTG traces and variability estimates; and fetal electrocardiogram waveform analysis is subject to promising new research. There is a clear need for close collaboration between computing and clinical experts. We believe that progress will be possible with multidisciplinary collaborative research

La inserción de los graduados 2011 y 2012 de la Universidad Nacional de La Matanza en el mercado laboral

La presente investigación es llevada a cabo por el Departamento de Ciencias de Económicas en conjunto con la Dirección de Graduados dependiente de la Secretaria de Extensión Universitaria de la Universidad Nacional de La Matanza. Este proyecto indaga acerca del vínculo graduado-inserción laboral de los graduados de la Universidad Nacional de La Matanza en relación a su titulación académica; y pretende ofrecer a la Institución un diagnóstico acerca del panorama laboral del graduado 2011 y 2012 de la Universidad. El fin de este trabajo es profundizar el proceso de seguimiento de los graduados en relación a la esfera laboral, dichos resultados junto a investigaciones futuras podrán influir en el diseño de políticas educativas institucionales orientados a la mejora continua. La realidad actual invita a ahondar a través de técnicas de investigación que permitan entender el dinamismo de los fenómenos que tienen lugar en la sociedad. A partir de una combinación de herramientas cuantitativas y cualitativas de recolección de información se pretende construir nuevo conocimiento que aporte a la mejora en la calidad educativa universitaria. Entre los instrumentos utilizados se pueden mencionar el análisis de base de datos, el relevamiento bibliográfico, la realización de encuestas y entrevistas, entre otras. En una primera instancia, se trabaja con técnicas que permiten conocer datos estructurados acerca de la situación laboral de los graduados, a través de encuestas, relevamiento y análisis de documentación estadística. En una segunda instancia, se orienta a profundizar la realidad existente que se pretende abordar, a través de un enfoque fenomenológico complementario, el cual intenta interpretar tanto el detalle de las vidas individuales como de los contextos sociales, políticos, y otros que sean pertinentes. Este proceso de investigación encausa la comprensión empática del fenómeno objeto de estudio y, si bien profundiza en las particularidades de cada sujeto, pone principal interés en su desempeño profesional y en su vínculo con el entorno laboral. De esta manera, se puede afirmar que la inserción laboral resulta un proceso complejo en el cual interviene no sólo la formación académica recibida sino variables sociales, económicas, entre otras. Por este motivo, resulta de vital importancia el acompañamiento que los graduados reciben de las instituciones que los forman, no sólo para brindarles una oferta de especialización académica vinculada a sus áreas de formación sino para guiarlos en el proceso y contenerlos en su inserción al mercado laboral. Como producto del trabajo de intervención originado por dicha investigación se ha diseñado y puesto en funcionamiento el Programa de Inclusión Laboral de Graduados de la Universidad Nacional de La Matanza. A través de este Proyecto, y sumado a la información relevada en esta investigación, se pretende conocer la realidad actual y, en función a ello, diseñar políticas educativas institucionales que acompañen y favorezcan la inserción laboral.Fil: Martínez, Julio Alejandro Nicolás. Universidad Nacional de La Matanza; Argentina.Fil: Otegui, Cristina. Universidad Nacional de La Matanza; Argentina.Fil: Mouta, Silvina Raquel. Universidad Nacional de La Matanza; Argentina.Fil: Faraldi, Ricardo. Universidad Nacional de La Matanza; Argentina.Fil: Galanti, Melody Nayla. Universidad Nacional de La Matanza; Argentina.Fil: Schifrin, Antonella. Universidad Nacional de La Matanza; Argentina.Fil: Varvuzza, Mailén Sofia Elisa. Universidad Nacional de La Matanza; Argentina.Fil: Feré, Maribel. Universidad Nacional de La Matanza; Argentina.Fil: Martínez, Nicolás. Universidad Nacional de La Matanza; Argentina.Fil: Rodríguez, Néstor Ariel. Universidad Nacional de La Matanza; Argentina.Fil: Pepe, María Laura. Universidad Nacional de La Matanza; Argentina.Fil: Santoriello, Hernán Carlos Marcelo. Universidad Nacional de La Matanza; Argentina.Fil: Marcos, Mariel Verónica. Universidad Nacional de La Matanza; Argentina.Fil: Lamas, Marcelo Oscar. Universidad Nacional de La Matanza; Argentina.Fil: Ibañez, Rosana Beatriz. Universidad Nacional de La Matanza; Argentina.Fil: Almaraz, Lorena Paola. Universidad Nacional de La Matanza; Argentina.Fil: Mansour, Jorge Alejandro. Universidad Nacional de La Matanza; Argentina.Fil: Santos, Edgard. Universidad Nacional de La Matanza; Argentina.Fil: Iribarne, Alba Esther. Universidad Nacional de La Matanza; Argentina.Fil: Turczak, Pedro. Universidad Nacional de La Matanza; Argentina

Characterization and structure-guided engineering of the novel versatile terpene monooxygenase CYP109Q5 from Chondromyces apiculatus DSM436

One of the major challenges in chemical synthesis is the selective oxyfunctionalization of non-activated C-H bonds, which can be enabled by biocatalysis using cytochrome P450 monooxygenases. In this study, we report on the characterization of the versatile CYP109Q5 from Chondromyces apiculatus DSM436, which is able to functionalize a wide range of substrates (terpenes, steroids and drugs), including the ring of β-ionone in non-allylic positions. The crystal structure of CYP109Q5 revealed flexibility within the active site pocket that permitted the accommodation of bulky substrates, and enabled a structure-guided approach to engineering the enzyme. Some variants of CYP109Q5 displayed a switch in selectivity towards the non-allylic positions of β-ionone, allowing the simultaneous production of 2- and 3-hydroxy-β-ionone, which are chemically challenging to synthesize and are important precursors for carotenoid synthesis. An efficient whole-cell system finally enabled the production of up to 0.5 g L-1 hydroxylated products of β-ionone; this system can be applied to product identification in further biotransformations. Overall, CYP109Q5 proved to be highly evolvable and active. The studies in this work demonstrate that, using rational mutagenesis, the highly versatile CYP109Q5 generalist can be progressively evolved to be an industrially valuable specialist for the synthesis of specific product