Effects of human chorionic gonadotropin intrauterine injection on oocyte retrieval day on assisted reproductive techniques outcomes: An RCT

Background: Several mediators play an important role in implantation. One of these mediators is human chorionic gonadotropin (HCG). Objective: To evaluate the effects of HCG intrauterine injection on the day of oocyte retrieval on the result of assisted reproductive techniques (ART). Materials and Methods: In this randomized clinical trial study, 126 women who were referred to Afzalipour Infertility Center between December 2018 to December 2019 undergoing in vitro fertilization/intracytoplasmic sperm injection cycles were enrolled and assigned to two groups of: a case (n = 62) and a control group (n = 64). The protocols for both groups were the same; except that the case group was injected with the protocols for both groups were the same, except that the case group was injected with 1000 IU of HCG into uterine cavity following the oocyte puncture, while no medication was administered to the control group. The implantation rate, chemical pregnancy, clinical pregnancy, and abortion rates were compared between the two groups. Results: Positive chemical pregnancy was seen in 15 (27.3%) cases of the case group and 14 (25.5%) of the control group. No significant difference was seen in the chemical and clinical pregnancy rates between the groups. The abortion rate was higher in the control group but that was not significant. Conclusion: A 1000 IU of HCG intrauterine injection after oocyte retrieval does not improve implantation, chemical or clinical pregnancy rates in ART cycles. Further studies are needed to clearly understand the role of HCG intrauterine injection in the day of oocyte retrieval in ART outcomes. Key words: Oocyte retrieval, Chorionic gonadotropin, Pregnancy, Assisted reproductive techniques

Effect of cement type and thickness on push-out bond strength of fiber posts

Background. This study investigated the effect of the thickness of two resin cements on push-out bond strength of fiber posts to root dentin. Methods. Sixty decoronated single-rooted teeth were endodontically treated. The specimens were then randomly allocated to two groups (n=30); group 1: drill size 90 w, matching the size of fiber posts used; group 2: drill size 110, larger than the posts. The specimens in each group were divided into 2 subgroups (n=15); subgroup SE, in which the posts were cemented with self-etch Panavia F2.0 and subgroup SA, in which self-adhesive Panavia cement was used. After 72 hours, 2 slices with 1 mm of thickness were prepared from the mid-root of each specimen. Push-out bond strength test was performed in a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with two-way ANOVA and t-test. Results. The mean bond strengths of the posts cemented in matched-size spaces for SE and SA groups were 4.02±1.6 and 4.12±2.3 MPa, respectively. For posts cemented in oversized spaces, the values were 4.9±2.3 and 2.8±1.3 MPa, respectively. In matched-size spaces, there was no significant difference between the two groups. Conclusion. The results of this study suggested that increasing the cement thickness would reduce the bond strength of fiber posts to root dentin when self-adhesive cements are used; however, self-etch adhesives were not affected

Efficacy of Low-Level Laser, Hard Occlusal Appliance and Conventional Pharmacotherapy in the Management of Myofascial Pain Dysfunction Syndrome; A Preliminary Study

Introduction:Myofascial pain dysfunction syndrome (MPDS) is a common temporomandibular joint disorder. Due to its multifactorial etiology, treatment usually involves more than one modality to obtain complementary results. The purpose of this study was to compare the combined effect of low level laser, hard occlusal appliance and conventional pharmacotherapy versus pharmacotherapy only in the management of patients with MPDS.Methods: In this randomized clinical trial, 15 MPDS patients were diagnosed and randomly assigned to 3 groups (n=5). Subjects in Group 1 were treated with pharmacotherapy (PT), Group 2 received active laser (940 nm Gallium Arsenide) every other day for a total of 10 sessions, plus pharmacotherapy (PTL) and Group 3 were given hard occlusal splint 12 h/day for 4 weeks plus pharmacotherapy (PTO). The intensity of pain was measured using visual analog scale (VAS) prior to treatment, 2 and 4 weeks after treatment onset and 2 weeks later. Maximum painless mouth opening and pain intensity at muscle palpation was also recorded. Comparisons were made between groups in 4 treatment sessions via repeated measure analysis of variance (ANOVA) (P < 0.05).Results: Pain relief, in subjective VAS was observed in both laser and appliance groups in the third and fourth examination sessions, though occlusal appliance significantly showed to provide the best results between the three groups (p<0.05). No statistically significant reduction in pain was noted using pharmacotherapy only. Maximum painless mouth opening and muscle tenderness was not significantly different among the three groups (p>0.05).Conclusion: Both Laser and occlusal appliance combined with pharmacotherapy proved to be effective for pain reduction. The 3 groups however failed to result in significant improvement in maximum mouth opening or tenderness of the muscles of mastication

Resistance to demineralisation of adjacent enamel and dentine, fluoride release and dentine bond strength of fluoride-containing self-etch adhesive systems

Background: The current study aimed to assess the amount of fluoride released from fluoride-containing dental adhesives and its effect on micro-tensile bond strength (?TBS) and on resistance to demineralisation of dentine and enamel.Material and Methods: Two fluoride-containing dental adhesives, and a fluoride-free adhesive were used as expe-rimental adhesives. After thermal cycling the ?-TBS of adhesives to dentine and the failure mode were assessed. The fluoride release and cross-sectional microhardness (CSMH) of specimens were measured before and after one day, 7 and 28 days of pH-cycling. The data were analysed using one-way ANOVA, Weibull statistics and repeated measures ANOVA. Results: The results indicated a significant difference between the group of FL and both the SE and LBF groups (p?0.001). The CSMH values of both the dentine and enamel underneath the adhesives was reduced at 28 th day of the pH-cycling compared to the baseline (p?0.001). From day 1 to day 28, the released fluoride declined in both the fluoride containing dental adhesives (p?0.001).Conclusions: Based on the results, the released fluoride from dental adhesives may adversely influence the bond strength and durability of the resin/dentine interface. Moreover, the released fluoride didn?t improve the resistance to demineralisation of adjacent enamel and dentine to bond interface

Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development

Purpose Recently, the upgrading of in vitro maturation (IVM) of human oocytes as a promising strategy has emerged in assisted reproductive technology (ART). The goal was to evaluate the correlation of the in vitro matured oocytes selected on the basis of the zona pellucida (ZP) birefringence and meiotic spindles (MS) detection with fertilization and subsequent embryo development in ICSI program. Methods A total of 168 immature oocytes [germinal vesicle (n = 140) and metaphase I (n = 28)] obtained from patients undergoing oocytes retrieval for ICSI. After in vitro culture for 24–40 h, 112 (67 %) oocytes reached to MII stage. Using a polarized microscopy, the presence of MS and ZP birefringence were assessed in matured oocytes, followed by ICSI performance. Results The rates of fertilization in oocytes with spindles (51.3 %) were similar to that of the oocytes without spindles (50.7 %; P = 1.00). Moreover, the fertilization rates in high birefringence (HB) oocytes was not statistically different than oocytes with low birefringence (LB) (P = 0.44). The findings also showed that 64.9 % of the fertilized oocytes developed to embryos, in which 33.3 % were derived from spindle-detected oocytes. Regarding the ZP birefringence, 35.5 % of the embryos were derived from HB oocytes. Conclusions There were insignificant relationships between the MS detection and ZP birefringence score with the rates of fertilization and embryo development in IVM oocytes

The global burden of cancer attributable to risk factors, 2010-19 : a systematic analysis for the Global Burden of Disease Study 2019

Background Understanding the magnitude of cancer burden attributable to potentially modifiable risk factors is crucial for development of effective prevention and mitigation strategies. We analysed results from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 to inform cancer control planning efforts globally. Methods The GBD 2019 comparative risk assessment framework was used to estimate cancer burden attributable to behavioural, environmental and occupational, and metabolic risk factors. A total of 82 risk-outcome pairs were included on the basis of the World Cancer Research Fund criteria. Estimated cancer deaths and disability-adjusted life-years (DALYs) in 2019 and change in these measures between 2010 and 2019 are presented. Findings Globally, in 2019, the risk factors included in this analysis accounted for 4.45 million (95% uncertainty interval 4.01-4.94) deaths and 105 million (95.0-116) DALYs for both sexes combined, representing 44.4% (41.3-48.4) of all cancer deaths and 42.0% (39.1-45.6) of all DALYs. There were 2.88 million (2.60-3.18) risk-attributable cancer deaths in males (50.6% [47.8-54.1] of all male cancer deaths) and 1.58 million (1.36-1.84) risk-attributable cancer deaths in females (36.3% [32.5-41.3] of all female cancer deaths). The leading risk factors at the most detailed level globally for risk-attributable cancer deaths and DALYs in 2019 for both sexes combined were smoking, followed by alcohol use and high BMI. Risk-attributable cancer burden varied by world region and Socio-demographic Index (SDI), with smoking, unsafe sex, and alcohol use being the three leading risk factors for risk-attributable cancer DALYs in low SDI locations in 2019, whereas DALYs in high SDI locations mirrored the top three global risk factor rankings. From 2010 to 2019, global risk-attributable cancer deaths increased by 20.4% (12.6-28.4) and DALYs by 16.8% (8.8-25.0), with the greatest percentage increase in metabolic risks (34.7% [27.9-42.8] and 33.3% [25.8-42.0]). Interpretation The leading risk factors contributing to global cancer burden in 2019 were behavioural, whereas metabolic risk factors saw the largest increases between 2010 and 2019. Reducing exposure to these modifiable risk factors would decrease cancer mortality and DALY rates worldwide, and policies should be tailored appropriately to local cancer risk factor burden. Copyright (C) 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license.Peer reviewe

Severe Bone Resorption Following Allogenic Bone Block Augmentation: A Case Report and Review of Literature

Allogeneic bone graft has been suggested to be a quite propitious alternative bone substitute to autograft. However, controlled clinical trials with adequate samples, including meticulous bone gain measurements, histological evaluations, and long-term clinical and radiographic assessments of inserted dental implants have not yet been conducted to evaluate the abovementioned assumption. This case report presents a patient for whom allogeneic bone block grafting with a guided bone regeneration procedure had been performed in order to improve the horizontal dimension of atrophic maxillary edentulous ridge. Following an uneventful 8-month healing period, though not obvious clinically, tomography images revealed a great amount of resorption, suggesting the replacement of bone graft with soft tissue. Histologic evaluation revealed no evidence of active bone formation. A mass of connective fibrous tissue was infiltrated with inflammatory cells. In between, the presence of trabecular bony structures with a distribution of empty lacunae was illustrative of graft remnants

Biomechanical Effects of Platform Switching in Two Different Implant Systems: A Three-Dimensional Finite Element Analysis

Aims: The purpose of this study was to determine the influence of platform switching on stress distribution of two different implant systems, using three-dimensional (3D) finite element models.Methods: Six 3D finite element models were created to replicate two different implant systems with peri-implant bone tissue, in which six different implant-abutment configurations were represented: model XiVE-a: 3.8-mm-diameter implant and 3.8-mm-diameter abutment; model XiVE-b (platform-switching model): 4.5-mm-diameter implant and 3.8-mm-diameter abutment; model XiVE-c: 4.5-mm-diameter implant and 4.5-mm-diameter abutment; model 3i-a: 4.0-mm-diameter implant and 4.1-mm-diameter abutment; model 3i-b (platform-switching model): 5.0-mm-diameter implant and 4.1-mm-diameter abutment; model 3i-c: 5.0-mm-diameter implant and 5.0-mm-diameter abutment. Axial and oblique loads of 100 were applied to all models.Results: While the pattern of stress distribution was similar for both loading situations, oblique loading resulted in higher intensity and greater distribution of stress than axial loading in both cortical bone and abutment-implant interface. Stress distribution at peri-implant bone was almost identical with similar magnitudes for all six models. In both implant systems, platform switching models demonstrated lower maximum von Mises stress in cortical bone than conventional models. However, in both implant systems and under both loading situation, platform switching models showed higher stresses at the abutment-implant interface than conventional models.Conclusion: In both implant systems, platform switching design reduced the stress concentration in the crestal bone and shifted it towards the area of implant-abutment interfac

Effect of Storage Time and Temperature on Dimensional Stability of Impressions Made with Zinc Oxide Impression Paste

Objectives: This study aimed to assess the effect of storage time and temperature on dimensional stability of impressions made with Cavex Outline zinc oxide impression paste. Materials and Methods: A round stainless steel mold with five grooves (three horizontal and two vertical) was used in this in-vitro experimental study. Cavex Outline impression paste was prepared according to the manufacturer’s instructions and applied to the mold. The mold was placed on a block and stored at 35°C and 100% humidity for setting. The impressions were poured with stone immediately and also after 30, 120, 240 and 420 minutes and 24 hours. The distance between the vertical lines on the casts was measured and compared with that in the immediately poured cast. Results: Storage in a refrigerator and at room temperature for zero to seven hours had no significant effect on dimensional stability of the impressions; however, 24 hours of storage in a refrigerator or at room temperature decreased the dimensional stability of Cavex Outline (P=0.001). Also, a significant association was found between dimensional changes following 24 hours of storage in a refrigerator (4°C) and at room temperature (23°C; P<0.01). Conclusions: The optimal pouring time of Cavex Outline impressions with stone is between zero to seven hours, and 24 hours of storage significantly decreases the dimensional stability.

Effect of Storage Conditions and Disinfection with 2% Deconex® on Dimensional Stability of Two Self-Cure Acrylic Resins

Background and Aim: There is a gap of information on the effect of time, disinfectant, and storage conditions on the dimensional stability of direct post patterns made of self-cure acrylic resins. This study assessed the effect of Deconex® and wet or dry stor-age conditions on the dimensional stability of post patterns made of Duralay and GC acrylic resins. Materials and Methods: Sixty specimens were fabricated of GC and Duralay acrylic resins (n=30) by using a stainless steel mold. The specimens in each group were divided into three subgroups (n=10). The specimens of subgroup 1 were immersed in Deconex® for 2 minutes followed by dry storage at 25°C. The specimens of subgroup 2 were stored in water at 25°C. The samples of subgroup 3 were immersed in Deconex® for 2 minutes followed by water storage at 25°C. The dimensions were measured immediately and 2 minutes, 1 hour, and 24 hours after setting. Data were analyzed by repeated measures ANOVA. One-way ANOVA and t-test were used for subgroup analysis at 5% significance level. Results: The greatest mean dimensional changes occurred after 24 hours in both groups. In GC group, subgroup 1 experienced minimal changes during 24 hours. Duralay subgroups 1 and 2 experienced minimal changes after 1 and 24 hours; the difference between the two subgroups was not significant. Maximum changes were noted in GC subgroups 2 and 3 and in Duralay subgroup 3 (P<0.05). Conclusion: In case of a 24-hour delay, storage conditions would have no effect on the dimensional stability of Duralay patterns, while GC patterns should be stored in a dry condition