Nitric Oxide Regulates Neurogenesis in the Hippocampus following Seizures

Hippocampal neurogenesis is changed by brain injury. When neuroinflammation accompanies injury, activation of resident microglial cells promotes the release of inflammatory cytokines and reactive oxygen/nitrogen species like nitric oxide (NO). In these conditions, NO promotes proliferation of neural stem cells (NSC) in the hippocampus. However, little is known about the role of NO in the survival and differentiation of newborn cells in the injured dentate gyrus. Here we investigated the role of NO following seizures in the regulation of proliferation, migration, differentiation, and survival of NSC in the hippocampus using the kainic acid (KA) induced seizuremouse model. We show that NO increased the proliferation of NSC and the number of neuroblasts following seizures but was detrimental to the survival of newborn neurons. NO was also required for the maintenance of long-term neuroinflammation. Taken together, our data show that NO positively contributes to the initial stages of neurogenesis following seizures but compromises survival of newborn neurons.Foundation for Science and Technology (FCT, Portugal); COMPETE; FEDER [PTDC/SAU-NEU/102612/2008, PTDC/NEU-OSD/0473/2012, PEst-C/SAU/LA0001/2013-2014, PEst-OE/EQB/LA0023/2013-2014]; FCT, Portugal [SFRH/BPD/78901/2011, SFRH/BD/77903/2011

Bond behavior of self consolidating concrete

The new generation of innovative projects has led to the use of greater amounts of reinforcement and the development of concrete with specific characteristics. It is necessary to use a material that ensures the uniformity of the cross section, as well as the adherence of the existing reinforcement, and due to this, the self-consolidating concrete becomes an technique alternative has great potential to achieve these properties. The aim of this paper is to analyze the bond behavior of self-consolidating concrete that was obtained by means of the Beam Test performed within a large experimental campaign of characterization. Four types of SCC were studied with two strength levels (40 MPa and 60MPa) and two different types of granular skeletons, using two specimens at each age (3, 7, and 28 days). All specimens were tested with a corrugated steel bar 10 mm in diameter. The results show that the adhesion tension independently of resistance presents a rapidly evolving at 7 days reached 95% of the total adhesion by 28 days.Postprint (published version

SSA of biomedical signals: A linear invariant systems approach

Singular spectrum analysis (SSA) is considered from a linear invariant systems perspective. In this terminology, the extracted components are considered as outputs of a linear invariant system which corresponds to finite impulse response (FIR) filters. The number of filters is determined by the embedding dimension.We propose to explicitly define the frequency response of each filter responsible for the selection of informative components. We also introduce a subspace distance measure for clustering subspace models. We illustrate the methodology by analyzing lectroencephalograms (EEG).FCT - PhD scholarship (SFRH/BD/28404/2006)FCT - PhD scholarship (SFRH/BD/48775/2008

Brand gender and consumer-based brand equity on Facebook: The mediating role of consumer-brand engagement and brand love

Brand gender has been suggested as a relevant source of consumer-based brand equity (CBBE). The purpose of this paper is to deepen understanding of the relationship between brand gender and CBBE by analyzing the mediating roleofconsumer–brandengagement (CBE)andbrandlove(BL)onthisrelationship.Thisresearchwas conducted on Facebook, the dominant global social media platform. The hypotheses were tested using structural equation modeling. Results support 6 of the 9 hypotheses, with a significant relationship between analyzed constructs. This study advances prior work by showing that brand gender has an indirect and relevant impact on CBBE through BL and CBE. Therefore, this research confirms the advantages of clear gender positioning and extends prior research by suggesting that brands with a strong gender identity will encourage BL and CB

Acoustic channel frequency response estimation using sources of opportunity

This work addresses the usage of ship radiated noise to estimate the ocean acoustic water propagation channel response between two vertical line arrays. We derive an expression for the frequency response channel estimate using a normal mode development based on cross-correlation methods, in a similar way as Roux et al. [1]. Its applicability and limitations in simulated and real conditions is discussed. Simulations are conducted using the normal mode model KRAKEN, based on the experimental setup and environmental parameters gathered during the RADAR' 07 sea trial, off the west coast of Portugal, in July of 2007. In this sea trial two drifting vertical line arrays with 16 and 8 hydrophones were deployed in a range independent bathymetric area, at 300 m and 1.3 km distance from the Research Vessel NRP D. Carlos I, whose track then moved away from the arrays, radiating noise in the frequency band bellow 750 Hz. The wave fronts structure, obtained from actual acoustic data of the above referred sea trial, reveals agreement with the simulations obtained with the proposed approach. These results suggest the feasibility of the method for future application in a passive ocean acoustics tomography framework to the estimation of sound speed perturbations in the water column

Sponge perforating lace coral with anticancer activity

This short note reports results from a pilot study to investigate new anticancer agents from deepsea corals in which colonizing sponges were encountered. The pure white stylasterid coral fans of Errina dabneyi (Pourtalès, 1871) are a conspicuous feature on the upper bathyal slopes in Azorean waters and can be found in depths from 215 to more than 500 m (Wisshak et al. 2009; Braga-Henriques et al. 2013). From the 26 species of Errina known worldwide (most from deeper waters) (Cairns 1983), E. dabneyi seems to be endemic to the Azores Archipelago and the adjacent Mid-Atlantic Ridge region (Zibrowius & Cairns 1992; Braga-Henriques et al. 2013). […]

Methodology to design an economic and strategic offshore wind energy Roadmap in Portugal

The main objective of this paper is to establish a roadmap for offshore wind energy in Portugal. It will determine the best sea areas to install fixed and floating offshore wind farms in this region, using spatial analysis of four economic indexes: Internal Rate of Return (IRR), Net Present Value (NPV), Discounted Pay-Back Period (DPBP) and Levelized Cost Of Energy (LCOE). Several economic parameters will be considered (Portuguese offshore tariff, investment and O&M costs, credit values, etc.).Three different discount rates were used into the sensitivity analysis. Several types of physical restrictions will be taking into account: submarine electrical cables, bathymetry, seabed geology, environmental conditions, protected areas in terms of heritage, navigation areas, seismic fault lines, etc. Moreover, location settings as proximity to shipyards or ports will be considered to complement the strategy. All of them will define the resulting area to install offshore wind farms along Portuguese coast. Spatial operations, considering economic, physical and strategic issues, have been carried out using Model Builder of GIS (Geographic Information Systems) software. Results indicate the Portuguese areas economically suitable for installing offshore wind farms

Policy Effects on the Quality of Public Health Care: Evaluating Portuguese Public Hospitals’ Quality through Customers’ Views

In this paper, public health care administration issues are reviewed and public hospital patients’ views on quality of health care are empirically tested. The purpose is to support the recommendation of new public policies that lead to better performance, if necessary. Hospital patients’ views on service quality were assessed through a questionnaire to estimate a global customer satisfaction measure. We argue that customer satisfaction should be measured through multiple indicators, as a latent variable. Thus, we considered the latent segment models (LSM) approach to assess customer service satisfaction. We found a two segment latent structure: segment 1, the satisfied, with 48 percent of patients, mostly male and middle-aged patients; and segment 2, the unsatisfied, with 52 percent of patients, mostly female and youngest/oldest patients.info:eu-repo/semantics/publishedVersio

A case study of product usability of a pelvic device used by children with neuromotor impairments

On assistive technology targeted for people with activity limitations and participation, usability issues becomes an essential tool to ensure that the product has the appropriate ergonomics characteristics, in other words, ensure that it fits the specific user´s needs. The aim of this study was to analyze the usability of an adaptive seating device for children with neuromotor impairments, by using kinematic indicators of the reaching movement. The study sample consisted of 13 children with associated neurologic conditions. The tests were developed by using a wooden bench height adjustable, integrated with the adaptive seating device under study, and a system to capture three-dimensional image, called Qualisys Track Manager. The following reaching kinematics variables were measured: maximum reaching velocity, movement duration, index of curvature, and unit movements. It was found that the use of the adaptive seating device had a positive impact on upper limb function in children with neuromotor impairments. It was also noticed an improvement in the reaching movement kinematics, which was statistical significant for the index of curvature and unit movements. As main conclusions, it is possible to point out some positive effects that the product under study seems to have on users' movements, such as the improved movement quality of the upper limb, which could mean a better postural adjustments and higher trunk postural control. By identifying new measures of usability in terms of effectiveness and efficiency for the analyzeddevice, the results obtained may serve also as performance indicators, providing new data that may help to improve the product and eventually modifying it, in order to turn it more compatible with the needs of the considered target population