Axion dark matter in a 3-3-1 model

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORSlightly extending a right-handed neutrino version of the 3 - 3 - 1 model, we show that it is not only possible to solve the strong CP problem but also to give the total dark matter abundance reported by the Planck collaboration. Specifically, we consider the possibility of introducing a 3 - 3 - 1 scalar singlet to implement a gravity stable Peccei-Quinn mechanism in this model. Remarkably, for allowed regions of the parameter space, the arising axions with masses m(a) approximate to meV can both make up the total dark matter relic density through nonthermal production mechanisms and be very close to the region to be explored by the IAXO helioscope.976112FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIOR2014/19164-6Sem informaçã

High bubble concentrations produced by ultrasounds in binary mixtures

7th Meeting of the European‐Society‐of‐Sonochemistry, BIARRITZ GUETHARY, FRANCE, MAY 14‐18, 2000International audienceIt was discovered that simultaneous insonification and air blowing of different aqueous binary solutions such as water/sodium‐dodecyl‐sulphate (SDS), water/methanol or water/potassium‐sulphate yields a very concentrated bubble cloud invading the whole vessel in a few seconds. After the end of insonification, this cloudiness remained in the solution for about 1 min. The phenomenon was investigated by computer‐treatment of solution pictures recorded every second after the end of insonification. Turbidity appeared to increase with ultrasound power, and also with SDS concentration. During the disappearance of the cloud, a turbidity front appeared rising and spreading upward. This front was studied in the characteristic plane and interpreted as a spatial segregation of different bubble sizes rising with different terminal velocities. The bubble sizes involved were estimated to about 10 mum. Adsorption of surface active species are invoked to explain the cloud formation and its abnormally slow disappearance, but the occurrence of the phenomenon for potassium‐sulphate salt remains unexplained

Asymmetric transfer hydrogenation by synthetic catalysts in cancer cells

Catalytic anticancer metallodrugs active at low doses could minimise side-effects, introduce novel mechanisms of action which combat resistance, and widen the spectrum of anticancer drug activity. We have used new highly-stable chiral half-sandwich organometallic Os(II) arene sulfonyl diamine complexes, [Os(arene)(TsDPEN)] to achieve highly enantioselective reduction of pyruvate, a key intermediate in metabolic pathways, both in aqueous model systems and in human cancer cells, using non-toxic concentrations of sodium formate as a hydride source. Importantly the catalytic mechanism generates selectivity towards ovarian cancer cells versus non-cancerous fibroblasts (both ovarian and lung), which are commonly used as models of healthy proliferating cells. The formate precursor N-formylmethionine was explored as an alternative to formate in PC3 prostate cancer cells, which are known to over-express a deformylase enzyme. Transfer hydrogenation catalysts generating reductive stress in cancer cells offer a ground-breaking new approach to cancer therapy

Bone growth during rapamycin therapy in young rats

<p>Abstract</p> <p>Background</p> <p>Rapamycin is an effective immunosuppressant widely used to maintain the renal allograft in pediatric patients. Linear growth may be adversely affected in young children since rapamycin has potent anti-proliferative and anti-angiogenic properties.</p> <p>Methods</p> <p>Weanling three week old rats were given rapamycin at 2.5 mg/kg daily by gavage for 2 or 4 weeks and compared to a Control group given equivalent amount of saline. Morphometric measurements and biochemical determinations for serum calcium, phosphate, iPTH, urea nitrogen, creatinine and insulin-growth factor I (IGF-I) were obtained. Histomorphometric analysis of the growth plate cartilage, in-situ hybridization experiments and immunohistochemical studies for various proteins were performed to evaluate for chondrocyte proliferation, chondrocyte differentiation and chondro/osteoclastic resorption.</p> <p>Results</p> <p>At the end of the 2 weeks, body and tibia length measurements were shorter after rapamycin therapy associated with an enlargement of the hypertrophic zone in the growth plate cartilage. There was a decrease in chondrocyte proliferation assessed by <it>histone-4 </it>and <it>mammalian target of rapamycin </it>(<it>mTOR</it>) expression. A reduction in <it>parathyroid hormone/parathyroid hormone related peptide (PTH/PTHrP) </it>and an increase in <it>Indian hedgehog </it>(<it>Ihh</it>) expression may explain in part, the increase number of hypertrophic chondrocytes. The number of TRAP positive multinucleated chondro/osteoclasts declined in the chondro-osseous junction with a decrease in the <it>receptor activator of nuclear factor kappa β ligand </it>(<it>RANKL</it>) and <it>vascular endothelial growth factor </it>(<it>VEGF</it>) expression. Although body and tibial length remained short after 4 weeks of rapamycin, changes in the expression of chondrocyte proliferation, chondrocyte differentiation and chondro/osteoclastic resorption which were significant after 2 weeks of rapamycin improved at the end of 4 weeks.</p> <p>Conclusion</p> <p>When given to young rats, 2 weeks of rapamycin significantly decreased endochondral bone growth. No catch-up growth was demonstrated at the end of 4 weeks, although markers of chondrocyte proliferation and differentiation improved. Clinical studies need to be done to evaluate these changes in growing children.</p

COVID-19 and Sick Leave: An Analysis of the Ibermutua Cohort of Over 1,651,305 Spanish Workers in the First Trimester of 2020

Objectives: The worldwide SARS-COV2 pandemic has impacted the health of workers and companies. The aim is to quantify it according to sick leave. Methods: Using ICD-9 codes, we analyzed Ibermutua records of all sick leaves during the first trimester of 2020, compared to during the same months of 2017, 2018, and 2019. We stratified the analysis by causes, patient sex, activity sectors, and regional data. All sick leaves were adjusted by the number of Ibermutua-affiliated persons in each period. Results: In March 2020, there was an unprecedented (116%) increase in total sick leaves, mainly due to infectious and respiratory diseases. Men and women were equally affected. All activity sectors were impacted, with the highest increase (457%) observed among health-related workers, especially due to contagious disease. The incidences of sick leaves were heterogeneous among different regions. Cost-analysis of sick leaves during the first trimester of 2020 compared with in previous years showed 40.3% increment (mean 2,813 vs. 2,005 euro per 100 affiliated workers). Conclusions: The SARS-COV2 pandemic is having a huge impact on workers' health, as shown by data regarding sick leaves in March 2020. This is associated with greater economic burden for companies, both due to the cost associated with sick leaves and the losses in productivity due to confinement

Comparison of proximal remote sensing devices of vegetable crops to determine the role of grafting in plant resistance to meloidogyne incognita

Proximal remote sensing devices are novel tools that enable the study of plant health status through the measurement of specific characteristics, including the color or spectrum of light reflected or transmitted by the leaves or the canopy. The aim of this study is to compare the RGB and multispectral data collected during five years (2016–2020) of four fruiting vegetables (melon, tomato, eggplant, and peppers) with trial treatments of non-grafted and grafted onto resistant rootstocks cultivated in a Meloidogyne incognita (a root-knot nematode) infested soil in a greenhouse. The proximal remote sensing of plant health status data collected was divided into three levels. Firstly, leaf level pigments were measured using two different handheld sensors (SPAD and Dualex). Secondly, canopy vigor and biomass were assessed using vegetation indices derived from RGB images and the Normalized Difference Vegetation Index (NDVI) measured with a portable spectroradiometer (Greenseeker). Third, we assessed plant level water stress, as a consequence of the root damage by nematodes, using stomatal conductance measured with a porometer and indirectly using plant temperature with an infrared thermometer, and also the stable carbon isotope composition of leaf dry matter.. It was found that the interaction between treatments and crops (ANOVA) was statistically different for only four of seventeen parameters: flavonoid (p < 0.05), NBI (p < 0.05), NDVI (p < 0.05) and the RGB CSI (Crop Senescence Index) (p < 0.05). Concerning the effect of treatments across all crops, differences existed only in two parameters, which were flavonoid (p < 0.05) and CSI (p < 0.001). Grafted plants contained fewer flavonoids (x¯ = 1.37) and showed lower CSI (x¯ = 11.65) than non-grafted plants (x¯ = 1.98 and x¯ = 17.28, respectively, p < 0.05 and p < 0.05) when combining all five years and four crops. We conclude that the grafted plants were less stressed and more protected against nematode attack. Leaf flavonoids content and the CSI index were robust indicators of root-knot nematode impacts across multiple crop typesY.H. acknowledges the support of the Tunisian government from the Ministery of Higher Education and Scientific Research. J.L.A. acknowledges support from the Institucio Catalana d'Investigacio i Estudis Avancats (ICREA) Academia, Generalitat de Catalunya, Spain. S.C.K. is supported by the Ramon y Cajal RYC-2019-027818-I research fellowship from the Ministerio de Ciencia e Innovacion, Spain. Thanks are also given to the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) for funding the project AGL2013-49040-C2-1-R and to the Ministry of Science and Innovation from the Spanish Government for funding the AGL2017-89785-R, and to the European Regional Development Fund (FEDER) AGL2017-89785-R, and for providing the FPI grant PRE2018-084265 to AMF. This research was also supported by the COST Action CA17134 SENSECO (Optical synergies for spatiotemporal sensing of scalable ecophysiological traits) funded by COST (European Cooperation in Science and Technology, www.cost.eu accessed on 29 April 2022)

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening

Directed evolution has revolutionized protein engineering. Still, enzyme optimization by random library screening remains sluggish, in large part due to futile probing of mutations that are catalytically neutral and/or impair stability and folding. FuncLib is a novel approach which uses phylogenetic analysis and Rosetta design to rank enzyme variants with multiple mutations, on the basis of predicted stability. Here, we use it to target the active site region of a minimalist-designed, de novo Kemp eliminase. The similarity between the Michaelis complex and transition state for the enzymatic reaction makes this system particularly challenging to optimize. Yet, experimental screening of a small number of active-site variants at the top of the predicted stability ranking leads to catalytic efficiencies and turnover numbers ( 2 104 M 1 s 1 and 102 s 1) for this anthropogenic reaction that compare favorably to those of modern natural enzymes. This result illustrates the promise of FuncLib as a powerful tool with which to speed up directed evolution, even on scaffolds that were not originally evolved for those functions, by guiding screening to regions of the sequence space that encode stable and catalytically diverse enzymes. Empirical valence bond calculations reproduce the experimental activation energies for the optimized eliminases to within 2 kcal mol 1 and indicate that the enhanced activity is linked to better geometric preorganization of the active site. This raises the possibility of further enhancing the stabilityguidance of FuncLib by computational predictions of catalytic activity, as a generalized approach for computational enzyme designKnut and Alice Wallenberg Foundation (Wallenberg Academy Fellowship) 2018.0140Human Frontier Science Program RGP0041/2017FEDER Funds/Spanish Ministry of Science, Innovation and Universities BIO2015-66426-R RTI2018-097142-B-100FEDER/Junta de Andalucia - Consejeria de Economia y Conocimiento E.FQM.113.UGR18Swedish National Infrastructure for computing (SNAC) 2018/2-3 2019/2-

Leptin Activates Human B Cells to Secrete TNF-α, IL-6, and IL-10 via JAK2/STAT3 and p38MAPK/ERK1/2 Signaling Pathway

Leptin, one of the adipokines, functions as a hormone and a cytokine. In this investigation, we show for the first time that leptin, in a concentration-dependent manner, activates human peripheral blood B cells to induce secretion of IL-6, IL-10, and TNF-α. Leptin increased B cells expressing CD25 and HLA-DR. Leptin induces phosphorylation of Janus activation kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), p38 mitogen-activated protein kinase (p38MAPK), and extracellular signal-regulated kinase (ERK1/2). Furthermore, leptin-induced cytokine secretion by B cells was blocked by inhibitors of JAK2, STAT3, p38MAPK, and ERK1/2. These data demonstrate that leptin activates human B cells to secrete cytokines via activation of JAK2/STAT3 and p38MAPK/ERK1/2 signaling pathways, which may contribute to its inflammatory and immunoregulatory properties