AGTEC-Org Technological Handbook of Methods

A common handbook was conceived in the CORE Organic AGTEC-Org project in order to give some elements on technological treatments and analyses which will be led in the project

ETUDE MORPHO-GRANULOMETRIQUE ET STRUCTURALE DES SEMOULES DE BLE DUR PROPRIETES D'HYDRATATION ET D'AGGLOMERATION

International audienceLes propriétés d'hydratation et d'agglomération de la semoule de blé dur dépendent des caractéristiques de la matière à granuler, du liquide de granulation et de l'outil de granulation. Dans le procédé de fabrication des graines de couscous, la formation, la croissance et la densification des grains de semoule sont effectuées par addition d'eau, mélange et roulage. Une étude de caractérisation de la semoule de blé dur a été entreprise à différentes échelles d'observation : macroscopique, mésoscopique et moléculaire afin de comprendre les mécanismes d'hydratation et d'agglomération. La semoule de blé dur constitue une population de particules hétérogènes en granulométrie et en composition biochimique. Si cette hétérogénéité se traduit par des modifications des propriétés d'hydratation, d'autres études sont nécessaires pour mieux appréhender son influence sur les propriétés d'agglomération

Impact of durum wheat milling on the deoxynivalenol distribution in the outcoming fractions

International audienceThe milling behavior of two naturally infected samples from durum wheat grains displaying contrasting levels of mycotoxins were analyzed. Although the two samples showed a similar milling behavior, an increase of about twenty percent in deoxynivalenol level was found in semolina from the sample containing the higher amount of mycotoxins. However, even if the highest concentration of deoxynivalenol was found in fractions originating from the grain outer layers, the mycotoxin contamination in semolina and flours were not related to the amount of two biochemical compounds (ash or phytic acid) that could be used as markers to monitor these external tissues. Presence of the trichothecene-producing fungi in the most internal semolina fraction was also shown using specific DNA primers and PCR amplification. Comparison between deoxynivalenol concentration in the feedings and corresponding output at each milling step or grinding of semolina fractions followed by sizing showed that mycotoxin concentration occurs in the finest particles at the first processing steps. Therefore, deoxynivalenol contamination of the milling fractions is not simply due to the presence of peripheral grain tissues

New challenges to improve organic bread wheat production in Europe

The total organic area in the EU-27 had an annual average growth rate of nearly 15% from 1998 to 2006 with winter wheat being the most important cereal crop. Wheat yield in organic farming is around 30% to 70% of yield of conventional farming but higher premia for organic wheat may to some extent compensate for this. Bread wheat is grown in a variety of crop rotations and farming systems and four basic organic crop production systems have been defined. Nitrogen deficiency and weed infestation are considered to be the most serious threat in organic wheat production. Organic wheat producers will have to fulfil the technological needs of bakers although the requirements differ widely from small artisan bakers to large enterprises handling the organic bread processing. To maintain and expand organic wheat production, there is a need to control weed population, manage nitrogen nutrition and maintain crop diversity in the cropping system. In order to obtain a share in the premium price of organic wheat products, farmers may involve in further processing and marketing

International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database - the quality controlled standard tool for routine identification of human and animal pathogenic fungi

Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections and creating new risks for established ones. There is a growing need for a rapid and accurate identification of pathogens to enable early diagnosis and targeted antifungal therapy. Morphological and biochemical identification methods are time-consuming and require trained experts. Alternatively, molecular methods, such as DNA barcoding, a powerful and easy tool for rapid monophasic identification, offer a practical approach for species identification and less demanding in terms of taxonomical expertise. However, its wide-spread use is still limited by a lack of quality-controlled reference databases and the evolving recognition and definition of new fungal species/complexes. An international consortium of medical mycology laboratories was formed aiming to establish a quality controlled ITS database under the umbrella of the ISHAM working group on "DNA barcoding of human and animal pathogenic fungi." A new database, containing 2800 ITS sequences representing 421 fungal species, providing the medical community with a freely accessible tool at http://www.isham.org and http://its.mycologylab.org/ to rapidly and reliably identify most agents of mycoses, was established. The generated sequences included in the new database were used to evaluate the variation and overall utility of the ITS region for the identification of pathogenic fungi at intra-and interspecies level. The average intraspecies variation ranged from 0 to 2.25%. This highlighted selected pathogenic fungal species, such as the dermatophytes and emerging yeast, for which additional molecular methods/genetic markers are required for their reliable identification from clinical and veterinary specimens.This study was supported by an National Health and Medical Research Council of Australia (NH&MRC) grant [#APP1031952] to W Meyer, S Chen, V Robert, and D Ellis; CNPq [350338/2000-0] and FAPERJ [E-26/103.157/2011] grants to RM Zancope-Oliveira; CNPq [308011/2010-4] and FAPESP [2007/08575-1] Fundacao de Amparo Pesquisa do Estado de So Paulo (FAPESP) grants to AL Colombo; PEst-OE/BIA/UI4050/2014 from Fundacao para a Ciencia e Tecnologia (FCT) to C Pais; the Belgian Science Policy Office (Belspo) to BCCM/IHEM; the MEXBOL program of CONACyT-Mexico, [ref. number: 1228961 to ML Taylor and [122481] to C Toriello; the Institut Pasteur and Institut de Veil le Sanitaire to F Dromer and D Garcia-Hermoso; and the grants from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) and the Fundacao de Amparo a Pesquisa do Estado de Goias (FAPEG) to CM de Almeida Soares and JA Parente Rocha. I Arthur would like to thank G Cherian, A Higgins and the staff of the Molecular Diagnostics Laboratory, Division of Microbiology and Infectious Diseases, Path West, QEII Medial Centre. Dromer would like to thank for the technical help of the sequencing facility and specifically that of I, Diancourt, A-S Delannoy-Vieillard, J-M Thiberge (Genotyping of Pathogens and Public Health, Institut Pasteur). RM Zancope-Oliveira would like to thank the Genomic/DNA Sequencing Platform at Fundacao Oswaldo Cruz-PDTIS/FIOCRUZ [RPT01A], Brazil for the sequencing. B Robbertse and CL Schoch acknowledge support from the Intramural Research Program of the NIH, National Library of Medicine. T Sorrell's work is funded by the NH&MRC of Australia; she is a Sydney Medical School Foundation Fellow.info:eu-repo/semantics/publishedVersio

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Chromatographie liquide à haute performance

National audienc

Anti-peptide antibodies directed against omega-gliadins for the detection of sequences from bread and durum wheats

International audienc

Grains de blé dur : importance de la résistance mécanique dans le comportement à la mouture

National audiencePour le blé dur (Triticum durum), la vitrosité des grains est une propriété capitale dans l’obtentionde rendement semoulier satisfaisant. Elle reflète la compaction des granules d’amidon et de lamatrice protéique. Les grains dont l’amande n’est pas vitreuse sont dits « mitadinés ». Un tauxde mitadin supérieur à 27% dans un lot de blé dur est un critère de déclassement du lot. Eneffet, le mitadin reflète une structure poreuse et moins résistante du grain qui générera doncplus de farines et moins de semoules à la mouture. Les blés mitadinés sont connus pour êtremoins riches en protéines et moins durs. La méthode de référence du taux de mitadin est uncomptage visuel sur des grains coupés. Dans cette étude, l’utilité de cette analyse de référencepour la prédiction du rendement en semoule est comparée à l’efficacité de prédictionseffectuées à partir de mesures des teneurs en protéines ou de duretés. Une trentaine de lotsde blés durs de variétés différentes, cultivés en Sud-Est et Sud-Ouest sur deux années, ont étécaractérisés pour leur vitrosité au farinotome de Pohl, leur teneur et composition en protéines,leur dureté PSI (Particle Size Index) et leur résistance mécanique (Hardness Index) mesurée auSKCS 4100 (Single Kernel Characterization System). Ces lots de qualités contrastées ont ensuiteété transformés dans une semoulerie semi-industrielle (150kg/h). Les proportions de semoulesobtenues ont été corrélées aux caractéristiques des blés durs afin de discuter du caractère plusou moins prédictif de chaque variable mesurée. L’identification de variables prédictives durendement semoulier apparait pertinente afin d’orienter la sélection des blés durs

Suivi de la cinétique thermique d'insolubilisation des protéines de la pâte alimentaire par chromatographie d'exclusion-diffusion haute performance

National audienc