The role of MIF in chronic lung diseases:Looking beyond inflammation

Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine that has been associated with many diseases. Most studies found in literature describe MIF as a proinflammatory cytokine involved in chronic inflammatory conditions, but evidence from last years suggests that many of its key effects are not directly related to inflammation. In fact, MIF is constitutively expressed in most human tissues and in some cases in high levels, which does not reflect the pattern of expression of a classic proinflammatory cytokine. Moreover, MIF is highly expressed during embryonic development and decreases during adulthood, which point toward a more likely role as growth factor. Accordingly, MIF knockout mice develop age-related spontaneous emphysema, suggesting that MIF presence (e.g., in younger individuals and wild-type animals) is part of a healthy lung. In view of this new line of evidence, we aimed to review data on the role of MIF in the pathogenesis of chronic lung diseases

Importancia de la conducta animal para el manejo productivo de la fauna silvestre y doméstica

The department of Sucre exhibits high biological diversity in terms of both, wild and domesticated species. In particular, the variety and quality of its cattle herds and other livestock are widely recognized. The growing productivity of these resources and the pressing need to preserve wild species in their natural habitats whilst continuing to make sustainable use of these assets, call for indepth knowledge of applicable biological, zootechnical and other related concepts. Disciplines such as Animal Behavior, for example, have generated very scarce scientific data. Through a detailed review and analysis of the available information , this paper demonstrates that a sound understanding of the ethology of both domestic and wild species is critical for their successful management and conservation. The study seeks to encourage research into the behavior of these animals and to provide a preliminary source for subsequent investigations in this areaEl departamento de Sucre presenta una gran diversidad biológica en cuanto a especies de fauna silvestre y también se distingue por la variedad y calidad de su ganado vacuno y otros domésticos. El incremento de la productividad de estos recursos, la necesidad de su conservación en estado silvestre y las posibilidades de explotación racional demandan conocimientos avanzados en el campo de la biología, la zootecnia y otras ramas afines. Una de las disciplinas donde se observan pocos resultados investigativos, es Comportamiento Animal. En este trabajo se demuestra, a través de la recopilación y análisis de la información disponible, la importancia que reviste el conocimiento de la etología de las especies de fauna, ya sean domésticas o silvestres, para su manejo productivo, conservación, o exhibición. Se pretende incentivar la realización de investigaciones sobre el comportamiento de especies domésticas y silvestres y brindar un punto de partida para la investigación acerca de este importante tema

Small-scale turbulence can reduce parasite infectivity to dinoflagellates

mall-scale turbulence and parasite infection are 2 important factors that govern the dynamics and fate of phytoplankton populations. We experimentally investigated the influence of turbulent mixing on the infectivity of the parasite Parvilucifera sinerae to dinoflagellates. Natural phytoplankton communities were collected during 3 stages of a bloom event in Arenys de Mar Har- bour (NW Mediterranean). The 15 to 60 μm size fraction was used as the inoculum and distributed into spherical flasks. Half of the recipients were exposed to turbulence while the rest were kept still. In the experiments, the dinoflagellate assemblage was mainly composed of Prorocentrum micans, Scrippsiella trochoidea and Alexandrium minutum. We observed a collapse of A. minutum and S. tro- choidea populations in the unshaken flasks, which coincided with an increase in parasite infectivity. After a short exposure to turbulence, the development of the dinoflagellate populations slowed down and stabilised as expected. In the shaken treatments, the infectivity was lower and the decay in the host cells numbers was delayed compared to the still treatments. The degree of interference of the turbulence with infectivity varied among the experiments, due to differences in cell abundances and possibly their physiological state. Results from a numerical model suggest that turbulence could lead to a 25 to 30% decrease in the maximum infection rate, which could be due to host population disper- sion and/or reduced host–parasite contact times. Turbulence may thus be effective in delaying the initial infection, but not in preventing it.Postprin

Arguments against the proposal 2302 by John & al. to reject the name Gonyaulax catenella (Alexandrium catenella)

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Explaining the polarized macrophage pool during murine allergic lung inflammation

IntroductionDifferentially polarized macrophages, especially YM1+ and MHCII+ macrophages, play an important role in asthma development. The origin of these polarized macrophages has not been elucidated yet. We therefore aimed to investigate how proliferation, monocyte recruitment, and/or switching of polarization states contribute to this specific pool of polarized interstitial and alveolar macrophages during development of house dust mite (HDM)-induced allergic lung inflammation in mice.MethodsMale and female mice were first treated intranasally with PKH26 to label lung-resident macrophages and were then exposed to either HDM or phosphate-buffered saline (PBS) for two weeks. Different myeloid immune cell types were quantified in lung tissue and blood using flow cytometry.ResultsWe found that macrophage polarization only starts up in the second week of HDM exposures. Before this happened, unpolarized alveolar and interstitial macrophages transiently increased in HDM-exposed mice. This transient increase was mostly local proliferation of alveolar macrophages, while interstitial macrophages also contained unlabeled macrophages suggesting monocyte contribution. After two weeks of exposures, the number of interstitial and alveolar macrophages was similar between HDM and PBS-exposed mice, but the distribution of polarization states was remarkably different. HDM-exposed mice selectively developed YM1+ alveolar macrophages and MHCII-hi interstitial macrophages while nonpolarized macrophages were lost compared to PBS-exposed mice. DiscussionIn this HDM model we have shown that development of a polarized macrophage pool during allergic inflammation is first dependent on proliferation of nonpolarized tissue-resident macrophages with some help of infiltrating unlabeled cells, presumably circulating monocytes. These nonpolarized macrophages then acquire their polarized phenotype by upregulating YM1 on alveolar macrophages and MHCII on interstitial macrophages. This novel information will help us to better understand the role of macrophages in asthma and designing therapeutic strategies targeting macrophage functions.</p

Quantifying the reductions in mortality from air-pollution by cancelling new coal power plants

Deep decarbonization paths to the 1.5 °C or 2 °C temperature stabilization futures require a rapid reduction in coal-fired power plants, but many countries are continuing to build new ones. Coal-fired plants are also a major contributor to air pollution related health impacts. Here, we couple an integrated human-earth system model (GCAM) with an air quality model (TM5-FASST) to examine regional health co-benefits from cancelling new coal-fired plants worldwide. Our analysis considers the evolution of pollutants control based on coal plants vintage and regional policies. We find that cancelling all new proposed projects would decrease air pollution related premature mortality between 101,388–213,205 deaths (2–5%) in 2030, and 213,414–373,054 (5–8%) in 2050, globally, but heavily concentrated in developing Asia. These health co-benefits are comparable in magnitude to the values obtained by implementing the Nationally Determined Contributions (NDCs). Furthermore, we estimate that strengthening the climate target from 2 °C to 1.5 °C would avoid 326,351 additional mortalities in 2030, of which 251,011 (75%) are attributable to the incremental coal plant shutdown.The authors acknowledge funding support from Bloomberg Philanthropies. This research is also supported by Basque Government through the BERC 2018-2021 and the Spanish Government through María de Maeztu excellence accreditation MDM-2017-0714. Jon Sampedro and Ignacio Cazcarro acknowledge financial support from the Ministry of the Economy and Competitiveness of Spain (RTI2018-099858-A-100 and RTI2018-093352-B-I00). Jon Sampedro acknowledge financial support from the Basque Government (PRE_2017_2_0139). The authors thank Patrick O’Rourke and Brinda Yarlagadda for their support with data processing. The authors declare no competing interests

The poor accuracy of D-dimer for the diagnosis of prosthetic joint infection but its potential usefulness in early postoperative infections following revision arthroplasty for aseptic loosening

Background: D-dimer was introduced in 2018 as an alternative biomarker for C-reactive protein (CRP) in the diagnostic of prosthetic joint infection (PJI) criteria of the Musculoskeletal Infection Society. We assessed the accuracy of plasma D-dimer for the diagnosis of early, delayed, and late PJI according to Infectious Diseases Society of America (IDSA) criteria, and whether persistently high levels of D-dimer in cases of aseptic loosening (AL) may be predictive of subsequent implant-related infection. Methods: A prospective study of a consecutive series of 187 revision arthroplasties was performed at a single institution.Septic (n = 39) and aseptic revisions (n = 141) were classified based on IDSA criteria. Preoperative assessment of CRP, erythrocyte sedimentation rate (ESR) and D-dimer was performed. Receiver operating curves were used to determine maximum sensitivity and specificity of the biomarkers. The natural progress of D-dimer for AL cases was followed up either until the date of implant-related infection at any time during the first year or 1 year after revision in patients without failure. Clinical outcomes for those AL cases included infection-related failure that required a new surgery or need for antibiotic suppression. Results: Preoperative D-dimer level was significantly higher in PJI cases than in AL cases (p = 0.000). The optimal threshold of D-dimer for the diagnosis of PJI was 1167 ng/mL. For overall diagnosis of PJI, C-reactive protein (CRP) achieved the highest sensitivity (84.6%), followed by erythrocyte sedimentation rate (ESR) and D-dimer (82% and 71.8%, respectively). Plasma D-dimer sensitivity was lower for all PJI types. When combinations of 2 tests were studied, the combined use of ESR and CRP achieved the best accuracy for all types of PJI (76.9%). 4.25% of AL cases had implant failure due to implant-related infection during the first year after the index revision arthroplasty, only the cases with early failure maintained high D-dimer levels

The toxicity and intraspecific variability of Alexandrium andersonii Balech

The toxicity of Alexandrium andersonii Balech is unclear and its intraspecific variability has yet to be studied. To address these gaps in our knowledge, in the present work five strains of A. andersonii from four different localities were characterized. The results showed that despite genetic homogeneity in the 5.8-ITS (internal transcribed spacer) and large subunit (LSU) regions and similar growth rates, strains originating from different locations varied with respect to cell size, the ratios of certain pigments, and their growth patterns. Cultures of the strains grown at 20 8C were analyzed for toxicity using four different methodologies. The two officially established methods, mouse bioassay and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and post-column reaction analysis of PSP toxins, failed to show the toxicity of any strain. Strains grown at 14 8C were also negative for PSP toxins by HPLC-FLD. However, strains grown at 20 8C exhibited both a response characteristic of the presence of toxin-inhibiting voltage-gated sodium channels, as demonstrated in a neuroblastoma neuro- 2a cell-based assay, as well as hemolytic activity in a sheep red blood cell assay

CUIDADOS A PERSONAS CON DISCAPACIDAD SEVERA. CASO CLÍNICO

It is known that the primary care nurse is responsible of domiciliary attention to those persons who are unable to care for him/herself due to illness or disability. Many times, they present very high levels of dependency reason why they are considered like great disabled. For that reason, the nurse must propose a nursing care plan in order to develop the autonomy of disabled person at the best possible levels; or, at least, to guard by the maintenance of its more basic necessities. For it, it is taught the caregiver, who is responsible for attending the daily needs of this patients. It is described the clinical case of a man with 24 years old who is totally dependent. In this case, a individual nursing care plan is developed. This plan includes desired outcomes and nursing interventions. The primary care nurse should pay special attention to the care of these persons.Se sabe que la enfermera de atención primaria es responsable de la atención domiciliaria a aquellas personas que son incapaces de cuidar de si mismos, debido a enfermedad o discapacidad. En muchas ocasiones presentan cotas de dependencia muy altas, los cuales son considerados como grandes discapacitados. Por ello, el profesional enfermero debe proponer un plan de cuidados enfermero, con el fin de desarrollar al máximo su autonomía; o, al menos, velar por el mantenimiento de sus necesidades más básicas. Para ello, se enseña al cuidador, que es el responsable de la atención de las necesidades diarias de estos pacientes. Se describe el caso clínico de un hombre de 24 años, que es totalmente dependiente. En este caso, se desarrolla un plan de cuidados enfermeros. Este plan incluye criterios de resultados e intervenciones enfermeras. La enfermera de atención primaria debe prestar especial atención a estas personas

Rules, Standards, and the Internal Point of View

Large scale structure and cosmolog