Preparation, Characterization and NO-CO Redox Reaction Studies over Palladium and Rhodium Oxides Supported on Manganese Dioxide

The catalytic activity of PdO/MnO2 and Rh2O3/MnO2 is investigated for NO-CO redox reaction. Supported catalysts are prepared by wet impregnation method. Among the tested catalysts, PdO/MnO2 shows higher activity for this reaction. Active metal dispersion on MnO2 enhances the selectivity for N2 over N2O in this reaction. The XRD substantiate the formation of MnO2 monophasic phase. SEM images show the formation of elongated particles. TEM images indicate nano-size rod-like morphologies. An increase in the catalytic activity is observed on supported Pd and Rh oxides on MnO2. Temperature programed desorption studies with NO and CO are undertaken to investigate the catalytic surface studies. © 2015 BCREC UNDIP. All rights reserve

Ectopic Pregnancy as a Model to Identify Endometrial Genes and Signaling Pathways Important in Decidualization and Regulated by Local Trophoblast

The endometrium in early pregnancy undergoes decidualization and functional changes induced by local trophoblast, which are not fully understood. We hypothesized that endometrium from tubal ectopic pregnancy (EP) could be interrogated to identify novel genes and pathways involved in these processes. Gestation-matched endometrium was collected from women with EP (n = 11) and intrauterine pregnancies (IUP) (n = 13). RNA was extracted from the tissue. In addition, tissues were prepared for histological analysis for degree of decidualization. We compared a) the samples from EP that were decidualized (n = 6) with non-decidualized samples (n = 5), and b) the decidualized EP (n = 6) with decidualization-matched IUP (n = 6) samples using an Affymetrix gene array platform, with Ingenuity Pathway Analysis, combined with quantitative RT-PCR. Expression of PRL and IGFBP1 was used to confirm the degree of decidualization in each group. There were no differences in PRL or IGFBP1 expression in the decidualization-matched samples but a marked reduction (P<0.001) in the non-decidualized samples. Decidualization was associated with increased expression of 428 genes including SCARA5 (181-fold), DKK1 (71-fold) and PROK1 (32-fold), and decreased expression of 230 genes including MMP-7 (35-fold) and SFRP4 (21-fold). The top canonical pathways associated with these differentially expressed genes were Natural Killer Cell and Wnt/b-Catenin signaling. Local trophoblast was associated with much less alteration of endometrial gene expression with an increase in 56 genes, including CSH1 (8-fold), and a reduction in 29 genes including CRISP3 (8-fold). The top associated canonical pathway was Antigen Presentation. The study of endometrium from tubal EP may promote novel insights into genes involved in decidualization and those influenced by factors from neighboring trophoblast. This has afforded unique information not highlighted by previous studies and adds to our understanding of the endometrium in early pregnancy

Decidual-Secreted Factors Alter Invasive Trophoblast Membrane and Secreted Proteins Implying a Role for Decidual Cell Regulation of Placentation

Inadequate or inappropriate implantation and placentation during the establishment of human pregnancy is thought to lead to first trimester miscarriage, placental insufficiency and other obstetric complications. To create the placental blood supply, specialized cells, the ‘extravillous trophoblast’ (EVT) invade through the differentiated uterine endometrium (the decidua) to engraft and remodel uterine spiral arteries. We hypothesized that decidual factors would regulate EVT function by altering the production of EVT membrane and secreted factors. We used a proteomics approach to identify EVT membrane and secreted proteins regulated by decidual cell factors. Human endometrial stromal cells were decidualized in vitro by treatment with estradiol (10−8 M), medroxyprogesterone acetate (10−7 M) and cAMP (0.5 mM) for 14 days. Conditioned media (CM) was collected on day 2 (non-decidualized CM) and 14 (decidualized CM) of treatment. Isolated primary EVT cultured on Matrigel™ were treated with media control, non-decidualized or decidualized CM for 16 h. EVT CM was fractionated for proteins <30 kDa using size-exclusion affinity nanoparticles (SEAN) before trypsin digestion and HPLC-MS/MS. 43 proteins produced by EVT were identified; 14 not previously known to be expressed in the placenta and 12 which had previously been associated with diseases of pregnancy including preeclampsia. Profilin 1, lysosome associated membrane glycoprotein 1 (LAMP1), dipeptidyl peptidase 1 (DPP1/cathepsin C) and annexin A2 expression by interstitial EVT in vivo was validated by immunhistochemistry. Decidual CM regulation in vitro was validated by western blotting: decidualized CM upregulated profilin 1 in EVT CM and non-decidualized CM upregulated annexin A2 in EVT CM and pro-DPP1 in EVT cell lysate. Here, non-decidualized factors induced protease expression by EVT suggesting that non-decidualized factors may induce a pro-inflammatory cascade. Preeclampsia is a pro-inflammatory condition. Overall, we have demonstrated the potential of a proteomics approach to identify novel proteins expressed by EVT and to uncover the mechanisms leading to disease states

Mechanistic approach to NO-CO reaction over some rare earth perovskites

863-864Catalytic oxidation reduction reaction between carbon monoxide (CO) and nitric oxide (NO) over perovskite type catalysts such as DyCoO3 and SmCoO3 have been studied from room temperature to 800 K, in a continuous flow fixed bed quartz reactor. Perovskites have been prepared by cobalticyanide method and characterized by XRD, surface area and electrical resistivity. Dy-CoO3 shows higher catalytic activity than SmCoO3 The mechanistic reaction path ways have been proposed

Zeolite supported iron catalyst for nitric oxide reduction by ammonia in the presence of oxygen

683-687Iron based H-ZSM-5 catalysts were used to study NO reduction with NH3 in the presence of oxygen with and without humid conditions. The iron based H-ZSM-5 was prepared by wet impregnation method and characterized by X-ray diffraction technique, BET surface area and SEM. TPD experiments showed low and high temperature NH3 desorption peaks. FTIR indicated only ammonia adsorbed species. Fe/ZSM-5 showed better NOx reduction with NH3 than H-ZSM-5 which was a poor catalyst for the NOx reduction. The catalytic activity did not show significant difference in the NOx conversion with and without water, but there was significant difference in NH3 conversion. It may be said that the dispersed Fe species and acid sites were the active centers for the reduction of NO with NH3 in the presence of oxygen

Selective catalytic reduction of nitric oxide with ammonia in presence of oxygen over Mn-ZSM5

303-306Selective catalytic reduction of NO with NH3 in the presence of oxygen with and without humid conditions has been studied over Mn-ZSM5 and H-ZSM5. The Mn-ZSM5 has been prepared by wet impregnation method, characterized by X-ray diffraction technique, atomic absorption spectroscopy and BET surface area. Mn-ZSM5 shows better NOx reduction than HZSM5. H-ZSM5 alone is a poor catalyst for NOx reduction with NH3, but metal incorporated catalyst becomes active. In the presence of humid condition Mn-ZSM5 gives lower NO3 and NH3 conversions. It may be concluded that the dispersed manganese species are the active centres for the selective catalytic reduction of NO with NH3 in the presence of oxygen

Catalytic reduction of NO_x by ammonia over nickel and lanthanum supported on zeolite

1167-1171The effects of oxygen and water on the reduction of NO by NH3 over metal supported H-ZSM-5 catalysts have been determined. The catalysts have been prepared by wet impregnation method and characterized by X-ray diffraction and BET surface area. Higher NOx reduction rate is obtained over Ni/ZSM-5 catalyst compared with La/ZSM-5 and H-ZSM-5. In presence of water vapour the NOx rate has been found to be lower than in the absence of water vapour. Oxygen promotes SCR reaction in forming reactive intermediate species for the reduction of NO by NH3

Synthesis, characterization and photocatalytic studies of some metal phthalocyanines

341-346Metal phthalocyanines (MPc) (M = Cu, Ni, Co and Fe) have been synthesized and characterized by XRD, IR, ESR and thermal technique. The solid state studies such as magnetic susceptibility, electrical resistivity and electron spin resonance have been carried out. Solar induced degradation of a textile dye Naphthol Blue Black (NBB) has been studied employing metal phthalocyanines