Mechanical Evaluation of Commercially Available Fibrin Sealants for Cartilage Repair.

OBJECTIVE: Fibrin sealants are routinely used for intra-articular surgical fixation of cartilage fragments and implants. However, the mechanical properties of fibrin sealants in the context of cartilage repair are unknown. The purpose of this study was to characterize the adhesive and frictional properties of fibrin sealants using an ex vivo model. DESIGN: Native bovine cartilage-bone composites were assembled with a single application of Tisseel or Vistaseal. Composites were tested in tension and lap shear. In addition, the coefficient of friction (COF) was measured in a native cartilage annulus model alone and with minced cartilage. Finally, the effect of a double application of fibrin sealant was evaluated. RESULTS: There were no significant differences in tensile modulus, ultimate tensile strength (UTS), shear modulus, or ultimate shear strength (USS) between the 2 fibrin sealants. Both fibrin sealants demonstrated a UTS and USS of <8 and <30 kPa, respectively. There were no differences in COF between the sealants when tested alone or with minced cartilage. A double application of fibrin sealant did not alter the mechanical properties compared with a single application of fibrin sealant. CONCLUSIONS: Fibrin sealant adhesive properties are not affected by the sealant type studied or the number of applications in a bovine cartilage-bone model. Fibrin sealant tribological properties are not affected by sealant type or the addition of minced cartilage. The adhesive properties of Tisseel and Vistaseal were less than those desired for the in vivo fixation of cartilage repair implants. These findings motivate the development of an improved cartilage-specific adhesive for cartilage repair applications

Sceloporus smithi Hartweg and Oliver, 1937 (Squamata: Phrynosomatidae): distributional range extension and a new elevation record from Oaxaca, México

A male Sceloporus smithi was collected at Hierve El Agua, in the Municipality of San Lorenzo Albarradas, Oaxaca, México. This record extends the known distribution of the species by approximately 25 km N from the closest acknowledged locality and also represents a new upward elevational record. Even though the elevation at this site is almost 700 m higher than for previously documented localities, the subhumid forest and igneous rock association is similar to other inhabited localities at lower elevations

Taklobo Tours: Conservation, Tourism and Livelihood Opportunities in the Island Garden City of Samal, Southern Philippines

Taklobo Tours was launched in 2013 at Adecor, Samal Island, Southern Philippines, to promote giant clam conservation, ecotourism, and provide livelihood to 19 fisherfolk beneficiaries. This is to address the observations that coastal communities in Barangay Adecor and other areas in Region XI have violated RA 8550 on the rules and regulations of the CITES-listed species, Tridacna spp., and that fisherfolk have low income. This study aims to determine the impacts of Taklobo Tours to conservation, tourism, and livelihood of beneficiaries. Qualitative and quantitative data from 2013 to 2015 were used to analyze and describe the impacts. On top of the existing in-situ conservation of giant clams in a marine protected area (MPA), capacity building, conservation advocacy, policy support, and site development were among the site interventions. To date, the beneficiaries have increased their level of participation as conservation advocates, violations are no longer observed in the barangay, and communities have shown strong support for the advocacy. Being the banner tourism project of the local government, it has an increasing number of guests from 284 per month in 2013 to 700 per month in 2015, but usually higher during peak months of April and May. A total of 11,611 local and international guests visited and contributed to the income from fees amounting to PhP835,195.00. This provided additional income of PhP50 to PhP700 per month in 2013 and PhP500 to PhP4,800 per month in 2015 to the beneficiaries and sustainability fund to the project. This remarkable journey was made possible through the strong partnership of academe with local government units of Island Garden City of Samal and Adecor for technical assistance, promotion, management, and legislations. Regular monitoring is recommended to ensure project sustainability

The herpetofauna of Hidalgo, Mexico: Composition, distribution, and conservation status

The herpetofauna of Hidalgo, Mexico, is comprised of 203 species, including 42 anurans, 17 caudates, one crocodylian, 137 squamates, and six turtles. Here, the distribution of the herpetofaunal species are catalogued among the four recognized physiographic regions. The total number of species varies from 77 in the Mexican Plateau to 166 in the Sierra Madre Oriental. The individual species occupy from one to four regions (mean = 2.1). About 69% of the Hidalgo herpetofauna is found in only one or two of the four regions, which is of considerable conservation significance. The greatest number of single-region species occupies the Sierra Madre Oriental (25), followed by the Gulf Coastal Lowlands (15), the Trans-Mexican Volcanic Belt (6), and the Mexican Plateau (2). The Coefficient of Biogeographic Resemblance (CBR) indicates that the Sierra Madre Oriental and the Gulf Coastal lowlands share the most species (72), because of their adjacent geographic position and they contain a significant number of generalist species that occur in the Gulf lowlands of Mexico, southern USA, Central America, and/or South America. The two largest geographic regions in Hidalgo by area, Sierra Madre Oriental and Mexican Plateau, reflect opposite patterns in species richness (166 and 77 species, respectively) due to overall differences in the ecological characteristics between them. A similarity dendrogram based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) depicts two distinct clusters, one between the Sierra Madre Oriental and Gulf Coastal Lowlands and the other between the Mexican Plateau and Trans-Mexican Volcanic Belt. The former cluster reflects the two regions sharing a substantial number of herpetofaunal species that occur on the Gulf lowlands of North America and Central America, as well as a few that enter South America. The second cluster is due to the two montane regions being adjacent to one another and their ecological similarities. With respect to the distributional categories, the largest number of species is that of the country endemics (104 of 203), followed by non-endemics (92), state endemics (four), and non-natives (three). The principal environmental threats to the Hidalgo herpetofauna are deforestation, livestock, roads, pollution of water sources, cultural factors, and diseases. The conservation status of each native species was assessed by means of the SEMARNAT (NOM-059), IUCN, and EVS systems, of which the EVS system was the most useful. The Relative Herpetofaunal Priority (RHP) method was also used to designate the rank order significance of the physiographic regions and the highest values were found for the Sierra Madre Oriental. Most of the five protected areas in Hidalgo are located in the Trans-Mexican Volcanic Belt, which is only the second most important region from a conservation perspective. In addition, only 78 of the 200 native species found in Hidalgo are recorded in total from the five protected areas. Finally, a set of conclusions and recommendations are offered for the future protection of the Hidalgo herpetofauna

The herpetofauna of Veracruz, Mexico: composition, distribution, and conservation status

The herpetofauna of the state of Veracruz, Mexico, currently consists of 359 species, including 76 anurans, 45 caudates, one caecilian, one crocodylian, 217 squamates, and 19 turtles. The distribution of the herpetofaunal species are catalogued here among the four recognized physiographic regions in the state. The total number of species ranges from 179 in the Sierra de Los Tuxtlas to 236 in the Sierra Madre Oriental. The number of species shared among the four physiographic regions ranges from 100 between the Gulf Coastal Lowlands and the Transmexican Volcanic Belt, to 190 between the Sierra Madre Oriental and the Transmexican Volcanic Belt. A similarity dendrogram based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) depicts two distinct clusters, one between the Sierra Madre Oriental and the Transmexican Volcanic Belt, and the other between the Gulf Coastal Lowlands and the Sierra de Los Tuxtlas. The former cluster refects two adjacent regions in highland environments that share a substantial number of herpetofaunal species, and the latter cluster shares a sizeable number of wide-ranging, generalist, lowland species found on the Atlantic and Pacifc versants of Mexico and Central America. The level of herpetofaunal endemism is relatively high, with 182 of 359 species either endemic to Mexico or to Veracruz. The distributional categorization of the total herpetofauna is as follows: 169 non-endemic species; 138 country endemic species; 44 state endemic species; and eight non-native species. The 169 non-endemic species are allocated to the following distributional categories: MXCA (89), MXSA (30), MXUS (29), USCA (11), USSA (four), and OCEA (fve). The principal environmental threats to the herpetofauna of Veracruz include deforestation, livestock, roads, water pollution, myths and other cultural factors, diseases, invasive species, and illegal commerce. The conservation status of each native species was evaluated using the SEMARNAT, IUCN, and EVS systems, of which the EVS system proved to be the most useful. The Relative Herpetofaunal Priority method was employed to determine the rank order signifcance of the four regions, and this identifed the Sierra Madre Oriental as the region of greatest importance. Only six protected areas exist in Veracruz, most of which are located in the Gulf Coastal Lowlands, the region of least conservation signifcance. The area of greatest signifcance, the Sierra Madre Oriental, does not contain any protected areas. A total of 265 species have been recorded within the six protected areas, of which 138 are non-endemics, 89 are country endemics, 31 are state endemics, and seven are non-natives. Finally, we provide a set of conclusions and recommendations to enhance the prospects for the future protection of the herpetofauna of Veracruz

An updated list of the Mexican herpetofauna: with a summary of historical and contemporary studies

The growth in our knowledge of the diversity of the herpetofauna of Mexico has occurred over the period of approximately 445 years from the work of Francisco Hernández to that of a broad multinational array of present-day herpetologists. The work of this huge group of people has established Mexico as one of the most significant centers of herpetofaunal biodiversity in the world. This status is the result of a complex orography, in addition to diverse habitats and environments and the biogeographic history of Mexico. The current herpetofauna consists of 1,421 native and introduced species, allocated to 220 genera, and 61 families. This figure is comprised of 1,405 native species and 16 non-native species (as of April 2023). The non-native species include two anurans, 13 squamates, and one turtle. The level of endemism is very high, presently lying at 63%, with this level expected to increase with time. Species richness varies among the 32 federal entities in the country, from a low of 50 in Tlaxcala to a high of 492 in Oaxaca. Amphibian species richness by state-level can be envisioned as comprising three levels of low, medium, and high, with the lowest levels occurring in the Peninsula of Baja California, a group of seven states in north-central and central Mexico, and a group of three states in the Yucatan Peninsula, with the highest levels occupying the southern states of Guerrero, Puebla, Veracruz, Oaxaca, and Chiapas, and the medium level in the remaining states of the country. Reptile species richness also can be allocated to three categories, with the lowest level occupying Baja California Sur, a group of central states, and the states of the Yucatan Peninsula, and the highest level found in a cluster of the states of Veracruz, Guerrero, Oaxaca, and Chiapas. Knowledge of the Mexican herpetofauna will continue to grow with additional studies on systematics, conservation, and the construction of checklists at various levels

The trans-ancestral genomic architecture of glycemic traits

Glycemic traits are used to diagnose and monitor type 2 diabetes and cardiometabolic health. To date, most genetic studies of glycemic traits have focused on individuals of European ancestry. Here we aggregated genome-wide association studies comprising up to 281,416 individuals without diabetes (30% non-European ancestry) for whom fasting glucose, 2-h glucose after an oral glucose challenge, glycated hemoglobin and fasting insulin data were available. Trans-ancestry and single-ancestry meta-analyses identified 242 loci (99 novel; P < 5 x 10(-8)), 80% of which had no significant evidence of between-ancestry heterogeneity. Analyses restricted to individuals of European ancestry with equivalent sample size would have led to 24 fewer new loci. Compared with single-ancestry analyses, equivalent-sized trans-ancestry fine-mapping reduced the number of estimated variants in 99% credible sets by a median of 37.5%. Genomic-feature, gene-expression and gene-set analyses revealed distinct biological signatures for each trait, highlighting different underlying biological pathways. Our results increase our understanding of diabetes pathophysiology by using trans-ancestry studies for improved power and resolution. A trans-ancestry meta-analysis of GWAS of glycemic traits in up to 281,416 individuals identifies 99 novel loci, of which one quarter was found due to the multi-ancestry approach, which also improves fine-mapping of credible variant sets.Peer reviewe

Genetic Drivers of Heterogeneity in Type 2 Diabetes Pathophysiology

Type 2 diabetes (T2D) is a heterogeneous disease that develops through diverse pathophysiological processes1,2 and molecular mechanisms that are often specific to cell type3,4. Here, to characterize the genetic contribution to these processes across ancestry groups, we aggregate genome-wide association study data from 2,535,601 individuals (39.7% not of European ancestry), including 428,452 cases of T2D. We identify 1,289 independent association signals at genome-wide significance (P \u3c 5 × 10-8) that map to 611 loci, of which 145 loci are, to our knowledge, previously unreported. We define eight non-overlapping clusters of T2D signals that are characterized by distinct profiles of cardiometabolic trait associations. These clusters are differentially enriched for cell-type-specific regions of open chromatin, including pancreatic islets, adipocytes, endothelial cells and enteroendocrine cells. We build cluster-specific partitioned polygenic scores5 in a further 279,552 individuals of diverse ancestry, including 30,288 cases of T2D, and test their association with T2D-related vascular outcomes. Cluster-specific partitioned polygenic scores are associated with coronary artery disease, peripheral artery disease and end-stage diabetic nephropathy across ancestry groups, highlighting the importance of obesity-related processes in the development of vascular outcomes. Our findings show the value of integrating multi-ancestry genome-wide association study data with single-cell epigenomics to disentangle the aetiological heterogeneity that drives the development and progression of T2D. This might offer a route to optimize global access to genetically informed diabetes care

Genetic drivers of heterogeneity in type 2 diabetes pathophysiology

Type 2 diabetes (T2D) is a heterogeneous disease that develops through diverse pathophysiological processes1,2 and molecular mechanisms that are often specific to cell type3,4. Here, to characterize the genetic contribution to these processes across ancestry groups, we aggregate genome-wide association study data from 2,535,601 individuals (39.7% not of European ancestry), including 428,452 cases of T2D. We identify 1,289 independent association signals at genome-wide significance (P &lt; 5 × 10-8) that map to 611 loci, of which 145 loci are, to our knowledge, previously unreported. We define eight non-overlapping clusters of T2D signals that are characterized by distinct profiles of cardiometabolic trait associations. These clusters are differentially enriched for cell-type-specific regions of open chromatin, including pancreatic islets, adipocytes, endothelial cells and enteroendocrine cells. We build cluster-specific partitioned polygenic scores5 in a further 279,552 individuals of diverse ancestry, including 30,288 cases of T2D, and test their association with T2D-related vascular outcomes. Cluster-specific partitioned polygenic scores are associated with coronary artery disease, peripheral artery disease and end-stage diabetic nephropathy across ancestry groups, highlighting the importance of obesity-related processes in the development of vascular outcomes. Our findings show the value of integrating multi-ancestry genome-wide association study data with single-cell epigenomics to disentangle the aetiological heterogeneity that drives the development and progression of T2D. This might offer a route to optimize global access to genetically informed diabetes care.</p

Ancestral diversity improves discovery and fine-mapping of genetic loci for anthropometric traits - the Hispanic/Latino Anthropometry Consortium

Hispanic/Latinos have been underrepresented in genome-wide association studies (GWAS) for anthropometric traits despite their notable anthropometric variability, ancestry proportions, and high burden of growth stunting and overweight/obesity. To address this knowledge gap, we analyzed densely-imputed genetic data in a sample of Hispanic/Latino adults to identify and fine-map genetic variants associated with body mass index (BMI), height, and BMI-adjusted waist-to-hip ratio (WHRadjBMI). We conducted a GWAS of 18 studies/consortia as part of the Hispanic/Latino Anthropometry (HISLA) Consortium (Stage 1, n=59,771) and generalized our findings in 9 additional studies (HISLA Stage 2, n=10,538). We conducted a trans-ancestral GWAS with summary statistics from HISLA Stage 1 and existing consortia of European and African ancestries. In our HISLA Stage 1+2 analyses, we discovered one BMI locus, as well as two BMI signals and another height signal each within established anthropometric loci. In our trans-ancestral meta-analysis, we discovered three BMI loci, one height locus, and one WHRadjBMI locus. We also identified three secondary signals for BMI, 28 for height, and two for WHRadjBMI in established loci. We show that 336 known BMI, 1,177 known height, and 143 known WHRadjBMI (combined) SNPs demonstrated suggestive transferability (nominal significance and effect estimate directional consistency) in Hispanic/Latino adults. Of these, 36 BMI, 124 height, and 11 WHRadjBMI SNPs were significant after trait-specific Bonferroni correction. Trans-ancestral meta-analysis of the three ancestries showed a small-to-moderate impact of uncorrected population stratification on the resulting effect size estimates. Our findings demonstrate that future studies may also benefit from leveraging diverse ancestries and differences in linkage disequilibrium patterns to discover novel loci and additional signals with less residual population stratification