555 research outputs found
Contraintes a l’adoption de la methode de l’hygiene sanitaire des vergers pour la lutte contre les mouches nuisibles aux fruits (Diptera, Tephritidae) par les producteurs de mangues et d’agrumes au Benin
No AbstractMots-clés: Bénin, hygiène sanitaire des vergers, mouches de fruits, producteurs de mangues et d’agrumes
Impacts of climate changes on risk zoning for cowpea in the Amazonian tropical conditions
The climate risk zoning was carried out for cowpea cultivated in northeastern Amazonia, Pará state, Brazil. Système d’Analyse
Régionale des Risques Agroclimatologiques (SARRA) crop model was used and calibrated from data obtained in field experiments
conducted between 2013 and 2016 in Castanhal, state of Pará. Low climate risk areas were defined as those with water requirement
satisfaction index (WRSI) greater than or equal to 0.5 during flowering and early grain development and less than 20 mm of total rainfall
during grain maturation and harvest for at least 80% of the simulated sowings of the crop. Simulations of the current and future climate
conditions were carried out with the regional climate modelling system version 4 (RegCM4) nested with the HadGEM2 global circulation
model with RCP 4.5 IPCC-AR4 scenario. The results show that climate changes are likely to reduce the duration of the optimal period for
sowing cowpea in the mentioned regioninfo:eu-repo/semantics/publishedVersio
Low-density Lipoprotein Cholesterol And Radiotherapy-induced Carotid Atherosclerosis In Subjects With Head And Neck Cancer
Background: Radiotherapy (RT) is a risk factor for accelerated carotid artery atherosclerotic disease in subjects with head and neck cancer. However, the risk factors of RT-induced carotid artery remodeling are not established. This study aimed to investigate the effects of RT on carotid and popliteal arteries in subjects with head and neck cancer and to evaluate the relationship between baseline clinical and laboratory features and the progression of RT-induced atherosclerosis.Findings: Eleven men (age = 57.9 ± 6.2years) with head and neck cancer who underwent cervical bilateral irradiation were prospectively examined by clinical and laboratory analysis and by carotid and popliteal ultrasound before and after treatment (mean interval between the end of RT and the post-RT assessment = 181 ± 47 days). No studied subject used hypocholesterolemic medications. Significant increases in carotid intima-media thickness (IMT) (0.95 ± 0.08 vs. 0.87 ± 0.05 mm; p 3.0.CO;2-X, 626923Pereira Lima, M.N., Biolo, A., Foppa, M., da Rosa, P.R., Rohde, L.E., Clausell, N., A prospective, comparative study on the early effects of local and remote radiation therapy on carotid intima-media thickness and vascular cellular adhesion molecule-1 in patients with head and neck and prostate tumors (2011) Radiother Oncol, 101, pp. 449-453. , 10.1016/j.radonc.2010.03.026, 20435360Kalábová, H., Melichar, B., Ungermann, L., Doležal, J., Krčmová, L., Kašparová, M., Plíšek, J., Solichová, D., Intima-media thickness, myocardial perfusion and laboratory risk factors of atherosclerosis in patients with breast cancer treated with anthracycline-based chemotherapy (2011) Med Oncol, 28, pp. 1281-1287. , 10.1007/s12032-010-9593-1, 20567943Muzaffar, K., Collins, S.L., Labropoulos, N., Baker, W.H., A prospective study of the effects of irradiation on the carotid artery (2000) Laryngoscope, 110, pp. 1811-1814. , 10.1097/00005537-200011000-00007, 11081590Faruolo, M., Fiorentino, A., Gallucci, G., Lapadula, L., Fusco, V., Intimal-medial thickness and carotid arteries lumen in irradiated patients for head and neck cancer: preliminary data of an observational study (2013) Clin Transl Oncol, 15, pp. 861-864. , 10.1007/s12094-013-1023-3, 23463595Ferreira-Sae, M.C., Cipolli, J.A., Cornélio, M.E., Matos-Souza, J.R., Fernandes, M.N., Schreiber, R., Costa, F.O., Nadruz, W., Sodium intake is associated with carotid artery structure alterations and plasma matrix metalloproteinase-9 upregulation in hypertensive adults (2011) J Nutr, 141, pp. 877-882. , 10.3945/jn.110.135921, 21430243Gemignani, T., Azevedo, R.C., Higa, C.M., Coelho, O.R., Matos-Souza, J.R., Nadruz, W., Increased popliteal circumferential wall tension induced by orthostatic body posture is associated with local atherosclerotic plaques (2012) Atherosclerosis, 224, pp. 118-122. , 10.1016/j.atherosclerosis.2012.06.069, 2281862
Redox transformations of adsorbed NO molecules on a Pt(100) electrode
The electrochemical behavior of adsorbed NO molecules on a Pt(100) electrode has been studied in perchloric acid solutions by means of cyclic voltammetry. According to the literature data, a saturated NO adlayer with a coverage of ∼0.5 monolayers (MLs) is formed under open circuit conditions in an acidic nitrite solution as a result of a disproportionation reaction. The saturated adlayer is stable in the potential range of 0.4–0.9 V vs. a reversible hydrogen electrode in 0.1 M HClO4. NO molecules are oxidized at 0.9–1.1 V with the formation of adsorbed nitrite anions, and they can be reduced to ammonia at potentials less than 0.4 V. In this paper it has been shown that the adlayer stability depends on the surface coverage and extent of ordering. An unsaturated NO adlayer demonstrates NO ↔ NH3 redox transformations at 0.5–0.8 V.Financial support from the Russian Foundation for Basic Research (project no. 10-03-00427), MICINN (CTR2010-1624)(Feder), and Generalitat Valenciana (Prometeo/2009/045)(Feder, Spain) is gratefully acknowledged
A Palatable Hyperlipidic Diet Causes Obesity and Affects Brain Glucose Metabolism in Rats
Background
We have previously shown that either the continuous intake of a palatable hyperlipidic diet (H) or the alternation of chow (C) and an H diet (CH regimen) induced obesity in rats. Here, we investigated whether the time of the start and duration of these feeding regimens are relevant and whether they affect brain glucose metabolism.
Methods
Male Wistar rats received C, H, or CH diets during various periods of their life spans: days 30-60, days 30-90, or days 60-90. Experiments were performed the 60th or the 90th day of life. Rats were killed by decapitation. The glucose, insulin, leptin plasma concentration, and lipid content of the carcasses were determined. The brain was sliced and incubated with or without insulin for the analysis of glucose uptake, oxidation, and the conversion of [1-14C]-glucose to lipids.
Results
The relative carcass lipid content increased in all of the H and CH groups, and the H30-60 and H30-90 groups had the highest levels. Groups H30-60, H30-90, CH30-60, and CH30-90 exhibited a higher serum glucose level. Serum leptin increased in all H groups and in the CH60-90 and CH30-90 groups. Serum insulin was elevated in the H30-60, H60-90, CH60-90, CH30-90 groups. Basal brain glucose consumption and hypothalamic insulin receptor density were lower only in the CH30-60 group. The rate of brain lipogenesis was increased in the H30-90 and CH30-90 groups.
Conclusion
These findings indicate that both H and CH diet regimens increased body adiposity independent treatment and the age at which treatment was started, whereas these diets caused hyperglycemia and affected brain metabolism when started at an early age
Expanded Bed Adsorption Of Bromelain (e.c. 3.4.22.33) From Ananas Comosus Crude Extract
This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25°C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain.261149157Amersham Pharmacia Biotech. EBA Handbook: Principles and Methods. Uppsala, ISBN 91-630-5519-8 (1997)Anspach, F.B., Curbelo, D., Hartman, R., Garke, G., Deckwer, W.D., Expanded-bed chromatography in primary protein purification (1999) J. Chromatogr. A, 865, p. 129Bruce, L.J., Chase, H.A., Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling (2001) Chem. Eng. Sci, 56, p. 3149Camprubi, S., Bruguera, M., Canalias, F., Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA). International (2006) J. Biol. Macromolecules, 38, pp. 134-139Chang, Y.K., McCreath, G.E., Chase, H.A., (1994) Advances in Bioprocess Engineering, p. 519. , Galindo, E, Ramirez, O.T, Eds, Kluwer Academics Publisher: Netherlands, pChase, H.A., Purification of proteins by adsorption chromatography in expanded beds (1994) Trends Biotechnol, 12, p. 296Dainiak, M.B., Galaev, I.Y., Matiasson, B., Direct capture of product from fermentation broth using a cell-repelling ion exchanger (2002) J. Chromatogr. A, 942, pp. 123-131Deutscher, M.P., Guide to protein purification (1990) Method. Enzymol, p. 182Fernandez-Lahore, H.M., Lin, D.-Q., Hubbuch, J.J., Kula, M.R., Thommes, J., The use of ionselective electrodes for evaluating residence time distributions in expanded bed adsorption systems (2001) Biotechnol. Progr, 17, pp. 1128-1136Gaspani, L., Limiroli, E., Ferrario, P., Bianchi, M., In vivo and in vitro effects of bromelain on PGE(2) and SP concentrations in the inflammatory exudate in rats (2002) Pharmacology, 65, pp. 83-86Haq, S.K., Rasheedi, S., Khan, R.H., Characterization of a partially folded intermediate of stem bromelain at low pH (2002) Eur. J. Biochem, 269, pp. 47-52Harrach, T., Eckert, K., Maurer, H.R., Machleidt, I., Machleidt, W., Nuck, R., Isolation and characterization of two forms of an acidic bromelain stem proteinase (1998) J. Protein Chem, 17, pp. 351-361Hatano, K., Sawano, Y., Tanokura, M., Structure-function relationship of bromelain isoinhibitors from pineapple stem (2002) Biol. Chem, 383, pp. 1151-1156Hatano, K., Tanokura, M., Takahashi, K., The amino acid sequences of isoforms of the bromelain inhibitor from pineapple stem (1998) J. Biochem, 124, pp. 457-461Hjorth, R., Expanded bed adsorption in industrial bioprocessing: Recent developments (1997) Trends Biotechnol, 15, p. 230Hochstrasser, D.F., Patchornik, A., Merril, C.R., Development of Polyacrylamide gels that improve the separation of proteins and their detection by silver staining (1998) Analyt. Biochem, 173, pp. 412-423Hochstrasser, D.F., Merril, C.R., Catalysts' for Polyacrylamide gel polymerization and detection of proteins by silver staining (1998) Appl. Theor. Electrophoresis, 1, pp. 35-40Hebbar, H.U., Sumana, B., Raghavarao, K.S.M.S., Use of reverse micellar systems for the extraction and purification of bromelain term from pineapple wastes (2008) Bioresource Technology, 99, pp. 4896-4902Khan, R.H., Rasheedi, S., Haq, S.K., Effect of pH, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain (2003) J. Biosci, 28, pp. 709-714Kim, M. H., Kim, H. K., Lee, J. K., Park, S. Y., Oh, T. 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B, 846, pp. 51-56Trinh, L., Phue, J.-N., Jaluria, P., Tsai, C.W., Narum, D.L., Shiloach, J., Screen-less expanded bed column: New approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris (2006) Biotechnol. Lett, 28, pp. 951-958Walter, H. E., Proteinases: methods with hemoglobin, casein, and azocoll as substrates. Pp. 270-277 in Methods of Enzymatic Analysis, 5, H.U. Bergmeyer, ed. Verlag Chemie, Weinheim, Germany (1984)Wang, S.-L., Peng, J.-H., Liang, T.-W., Liu, K.-C., Purification and characterization of a chitosanase from Serratia marcescens TKU011 (2008) Carbohydrate Research, 343, pp. 1316-1323Wharton, C.W., The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester (1974) Biochem. 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A Novel Hybrid Feature Selection Algorithm for Hierarchical Classification
Feature selection is a widespread preprocessing step in the data mining field. One of its purposes is to reduce the number of original dataset features to improve a predictive model’s performance. Despite the benefits of feature selection for the classification task, to the best of our knowledge, few studies in the literature address feature selection for the hierarchical classification context. This paper proposes a novel feature selection method based on the general variable neighborhood search metaheuristic, combining a filter and a wrapper step, wherein a global model hierarchical classifier evaluates feature subsets. We used twelve datasets from the proteins and images domains to perform computational experiments to validate the effect of the proposed algorithm on classification performance when using two global hierarchical classifiers proposed in the literature. Statistical tests showed that using our method for feature selection led to predictive performances that were consistently better than or equivalent to that obtained by using all features with the benefit of reducing the number of features needed, which justifies its efficiency for the hierarchical classification scenario
Prioritizing Amazon Forest conservation: Assessing potential biomass under climate change
This study aims to identify the capacity of intact forests to maintain Above Ground Biomass (AGB) under new climate conditions. Using a predictive approach under different climate scenarios (SSP1–2.6 and SSP5–8.5), and considering the mean of General Circulation Models (GCMs) for the period (2021–2040), it was revealed that the regions with the greatest capacity for AGB in the optimistic scenario are concentrated in the northwest and southeast regions, covering the basins of the Negro, Xingu and Tapajós rivers. In the SSP5–8.5 scenario, although potential AGB gains are concentrated in the same regions, the spatial distribution is more restricted and dispersed. Indigenous Landsemerge as areas with the greatest potential AGB gains in both climate scenarios. However, these predictions do not include the impact of extreme climate events, such as storms, severe droughts, and wildfires, which could lead to an underestimation of actual biomass variations
TEMPO NECESSÁRIO PARA NOVILHAS NELORE CRIADAS A PASTO, GANHAREM 210, 240, 270 KG DESDE O NASCIMENTO
O objetivo foi avaliar o número de dias para novilhas da raça Nelore (4.577) ganharem
210, 240 e 270 kg a partir do nascimento, criadas em regime de pasto. Para as análises estatísticas,
utilizou-se o método dos quadrados mínimos. O modelo continha os efeitos fixos de mês e ano de
nascimento da novilha e a idade da mãe como covariável; como efeito aleatório, touro aninhado
dentro de fazenda e o erro. As respostas mostraram que é possível desafiar fêmeas mais jovens,
com pesos menores, quando lhes são asseguradas boas condições nutricionais, e assim permitir
que seu crescimento não seja interrompido.
Days from birth to get 210, 240, 270 kg for nellore heifers on grazing
Abstract
The objective of this study was to evaluate the number of days for 4,577 nellore
heifers raised in regimen of grass to get 210, 240 and 270 kg of weight since the birth. For statistical
analyses, the square means method was used. The model contained the fixed effects from helfer´s
month and year of birth and the dam´s age as co-variable; as random effect, bull sheltered inside
the farm and the error. The results showed that it is possible to challenge younger females, with
lower weight, when good nutritional conditions are assured, allowing uninterrupted growth
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