132 research outputs found

    Simultaneous Cassini VIMS and UVIS observations of Saturn's southern aurora: Comparing emissions from H, H-2 and H-3(+) at a high spatial resolution

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    Here, for the first time, temporally coincident and spatially overlapping Cassini VIMS and UVIS observations of Saturn's southern aurora are presented. Ultraviolet auroral H and H-2 emissions from UVIS are compared to infrared H-3(+) emission from VIMS. The auroral emission is structured into three arcs - H, H-2 and H-3(+) are morphologically identical in the bright main auroral oval (similar to 73 degrees S), but there is an equatorward arc that is seen predominantly in H (similar to 70 degrees S), and a poleward arc (similar to 74 degrees S) that is seen mainly in H-2 and H-3(+). These observations indicate that, for the main auroral oval, UV emission is a good proxy for the infrared H-3(+) morphology (and vice versa), but for emission either poleward or equatorward this is no longer true. Hence, simultaneous UV/IR observations are crucial for completing the picture of how the atmosphere interacts with the magnetosphere

    Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone

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    Plant cell wall composition is important for regulating growth rates, especially in roots. However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates. This study reveals the benefits of carrying out multiple analyses in combination. Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates. We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots. Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones. Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes. Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele. The transcriptome analysis found several gene families peaking in the REZ. These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19). The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion. Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth
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