Early Maladaptive Schemas among children: a new way to screen for depressed child

How can children’s schemas evolve into adult schemas that are responsible for depression? To answer this question, we translated Schmidt, Joiner, Young, and Telch’s (1995) Early Maladaptive Schema Questionnaire into French and adapted it to children. We administered the questionnaire to two groups of children in years seven to ten (N = 252), one group containing children suffering from depression and the other containing children with no psychiatric disorders. The results provided insight into the structure of depression schemas. From a clinical perspective, we stress the possibility of using this tool to individually or collectively detect «normal» and «abnormal» schemas in children Early maladaptive schemas among children: a new way to screen for depressed child

Purging the HIV-1 reservoir through the disruption of the PD-1 pathway

International audiencen.

Microparticle-mediated transfer of the viral receptors CAR and CD46, and the CFTR channel in a CHO cell model confers new functions to target cells

Cell microparticles (MPs) released in the extracellular milieu can embark plasma membrane and intracellular components which are specific of their cellular origin, and transfer them to target cells. The MP-mediated, cell-to-cell transfer of three human membrane glycoproteins of different degrees of complexity was investigated in the present study, using a CHO cell model system. We first tested the delivery of CAR and CD46, two monospanins which act as adenovirus receptors, to target CHO cells. CHO cells lack CAR and CD46, high affinity receptors for human adenovirus serotype 5 (HAdV5), and serotype 35 (HAdV35), respectively. We found that MPs derived from CHO cells (MP-donor cells) constitutively expressing CAR (MP-CAR) or CD46 (MP-CD46) were able to transfer CAR and CD46 to target CHO cells, and conferred selective permissiveness to HAdV5 and HAdV35. In addition, target CHO cells incubated with MP-CD46 acquired the CD46-associated function in complement regulation. We also explored the MP-mediated delivery of a dodecaspanin membrane glycoprotein, the CFTR to target CHO cells. CFTR functions as a chloride channel in human cells and is implicated in the genetic disease cystic fibrosis. Target CHO cells incubated with MPs produced by CHO cells constitutively expressing GFP-tagged CFTR (MP-GFP-CFTR) were found to gain a new cellular function, the chloride channel activity associated to CFTR. Time-course analysis of the appearance of GFP-CFTR in target cells suggested that MPs could achieve the delivery of CFTR to target cells via two mechanisms: the transfer of mature, membrane-inserted CFTR glycoprotein, and the transfer of CFTR-encoding mRNA. These results confirmed that cell-derived MPs represent a new class of promising therapeutic vehicles for the delivery of bioactive macromolecules, proteins or mRNAs, the latter exerting the desired therapeutic effect in target cells via de novo synthesis of their encoded proteins

Photoelectrochemical characterization of electrodeposited polyaniline

Photoelectrochemistry is based on interfacial electron transfer, where one of the phases is the excited state of a semiconductor. We used the a.c. impedance technique in order to investigate these interface processes. Polyaniline films galvanostatically grown on platinum electrodes showed variation of photocurrent responses with d.c. bias with two maxima, 0.4 and 0.7 V, and a minimum at 0.65 V. Electrochemical impedance spectra were used to model the interface in terms of the variation of space-charge layer capacitance with d.c. bias, observed in lower frequencies. The flat-band potential, in the order of 0.65 V versus Ag/AgCl, was determined from Mott-Schottky plots and was also observed in photoelectrochemical experiments. From the slope of the Mott-Schottky plot we observed that the charge carriers concentration is higher when polyaniline is irradiated. The polyaniline gap, 2.85 eV, was determined by the Kubelka-Munk function. (C) 1997 Elsevier Science S.A.89316716

PD-1 blockade potentiates HIV latency reversal ex vivo in CD4⁺ T cells from ART-suppressed individuals.

HIV persists in latently infected CD4 <sup>+</sup> T cells during antiretroviral therapy (ART). Immune checkpoint molecules, including PD-1, are preferentially expressed at the surface of persistently infected cells. However, whether PD-1 plays a functional role in HIV latency and reservoir persistence remains unknown. Using CD4 <sup>+</sup> T cells from HIV-infected individuals, we show that the engagement of PD-1 inhibits viral production at the transcriptional level and abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells. Conversely, PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production in combination with the latency reversing agent bryostatin without increasing T cell activation. Our results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency disruption

Pricing variance swaps under the Hawkes jump-diffusion process

This paper presents an analytical approach for pricing variance swaps with discrete sampling times when the underlying asset follows a Hawkes jump-diffusion process characterized with both stochastic volatility and clustered jumps. A significantly simplified method, with which there is no need to solve partial differential equations, is used to derive a closed-form pricing formula. A distinguished feature is that many recently published formulas can be shown to be special cases of the one presented here. Some numerical examples are provided with results demonstrating that jump clustering indeed has a significant impact on the price of variance swaps

Cross-clade ultrasensitive PCR-based assays to measure HIV persistence in large-cohort studies

A small pool of infected cells persists in HIV-infected individuals receiving antiretroviral therapy (ART). Here, we developed ultrasensitive assays to precisely measure the frequency of cells harboring total HIV DNA, integrated HIV DNA, and two long terminal repeat (2-LTR) circles. These assays are performed on cell lysates, which circumvents the labor-intensive step of DNA extraction, and rely on the coquantification of each HIV molecular form together with CD3 gene sequences to precisely measure cell input. Using primary isolates from HIV subtypes A, B, C, D, and CRF01_A/E, we demonstrate that these assays can efficiently quantify low target copy numbers from diverse HIV subtypes. We further used these assays to measure total HIV DNA, integrated HIV DNA, and 2-LTR circles in CD4(+) T cells from HIV-infected subjects infected with subtype B. All samples obtained from ART-naive subjects were positive for the three HIV molecular forms (n = 15). Total HIV DNA, integrated HIV DNA, and 2-LTR circles were detected in, respectively, 100%, 94%, and 77% of the samples from individuals in which HIV was suppressed by ART. Higher levels of total HIV DNA and 2-LTR circles were detected in untreated subjects than individuals on ART (P = 0.0003 and P = 0.0004, respectively), while the frequency of CD4(+) T cells harboring integrated HIV DNA did not differ between the two groups. These results demonstrate that these novel assays have the ability to quantify very low levels of HIV DNA of multiple HIV subtypes without the need for nucleic acid extraction, making them well suited for the monitoring of viral persistence in large populations of HIV-infected individuals