Real-scale investigation of the kinematic response of a rockfall protection embankment

This paper addresses the response of rockfall protection embankments when exposed to a rock impact. For this purpose, real-scale impact experiments were conducted with impact energies ranging from 200 to 2200 kJ. The structure was composed of a 4m high cellular wall leaned against a levee. The wall was a double-layer sandwich made from gabion cages filled with either stones or a sand–schreddedtyre mixture. For the first time, sensors were placed in different locations within the structure to measure real-time accelerations and displacements. The test conditions, measurement methods and results are presented in detail. The structure’s response is discussed in a descriptive and phenomenological approach and compared with previous real-scale experiments on other types of embankments

Instabilities of a sand layer subjected to an upward water flow by a 2D coupled discrete element - Lattice Boltzmann hydromechanical model

This work deals with the numerical simulation of the instabilities occurring in a sand layer subjected to an upward water flow. A coupled Discrete Elements - Lattice Boltzmann hydromechanical model is used for this end. After a brief presentation of the numerical model, simulations of an upward fluid flow through granular deposits are performed for two cases namely under controlled hydraulic gradients and under controlled volumetric flow rates. In the first case i.e. under controlled hydraulic gradient, the simulations show that the quicksand condition is actually reached for a hydraulic gradient very close to the critical hydraulic gradient calculated from the global analysis of classical soil mechanics. The simulations point out moreover that the quicksand phenomenon could be produced locally under slightly lower gradients. In the second case i.e. under controlled volumetric flow rates, the simulations show that there are three levels of flow ; low flow rates that allow infiltration without any destabilization, medium flow rates that cause expansion of the deposit to increase its permeability and high flow rates which may cause the formation continuous tunnel between the upstream and the downstream sides as well as sand boils. It is shown also that under the controlled flow rate condition the hydraulic gradient remains in all cases less than the average critical hydraulic gradient

Variability in the durability of CRISPR-Cas immunity

This is the author accepted manuscript. The final version is available from Royal Society via the DOI in this record.The durability of host resistance is challenged by the ability of pathogens to escape the defence of their hosts. Understanding the variability in the durability of host resistance is of paramount importance for designing more effective control strategies against infectious diseases. Here, we study the durability of various clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) alleles of the bacteria Streptococcus thermophilus against lytic phages. We found substantial variability in durability among different resistant bacteria. Since the escape of the phage is driven by a mutation in the phage sequence targeted by CRISPR-Cas, we explored the fitness costs associated with these escape mutations. We found that, on average, escape mutations decrease the fitness of the phage. Yet, the magnitude of this fitness cost does not predict the durability of CRISPR-Cas immunity. We contend that this variability in the durability of resistance may be because of variations in phage mutation rate or in the proportion of lethal mutations across the phage genome. These results have important implications on the coevolutionary dynamics between bacteria and phages and for the optimal deployment of resistance strategies against pathogens and pests. Understanding the durability of CRISPR-Cas immunity may also help develop more effective gene-drive strategies based on CRISPR-Cas9 technology. This article is part of a discussion meeting issue 'The ecology and evolution of prokaryotic CRISPR-Cas adaptive immune systems'.Natural Environment Research Council (NERC)Biotechnology & Biological Sciences Research Council (BBSRC)European CommissionEuropean Molecular Biology Organization (EMBO)Leverhulme TrustNatural Sciences and Engineering Research Council of Canad

Caractérisation pathologique in vitro de deightoniella torulosa (Syd.) Ellis sur les cultivars de bananiers figue sucrée (musa AA), grande naine (musa AAA) et orishele (musa AAB)

Deightoniella torulosa est un champignon fréquemment associé aux attaques des cercosporioses sur les principaux cultivars de bananier en Côte-d\'Ivoire. Les symptômes qu\'il induit sont mal connus et les attaques occasionnées en plantation sont négligées. Le champignon a été isolé et son pouvoir pathogène a été mis en évidence sur des fragments de feuilles et des feuilles entières de différents cultivars de bananiers en utilisant différentes concentrations de spores : à 104, 5.104 et 105 spores / ml. Des coupes histologiques ont été aussi réalisées. Les résultats ont mis en évidence l\'activité parasitaire du champignon sur les fragments de feuilles et sur les feuilles entières. La période d\'incubation est de 3 à 5 jours. La germination des spores débute en moins d\'une heure et atteint 95 à 100 % au bout de 4 heures. L\'évolution des symptômes varie en fonction des cultivars. L\'ampleur des attaques s\'est révélée importante lorsque les concentrations de spores sont élevées. Le champignon provoque des attaques sévères pour des concentrations de 105 spores / ml. Ainsi, Deightoniella torulosa induit seul des attaques, colonise les tissus du limbe foliaire et contribue à la sénescence prématurée des feuilles de bananiers.Deightoniella torulosa is a fungus usually associated with symptoms of black sigatoka leaf streak in Côte-d\'Ivoire. The pathogenic activity of the fungus is not well known and its effect on field is neglected. The fungus has been isolated and its pathogenic activity has been determined on leaves of cultivated banana through the use of different concentrations of conidia: 104, 5.104 and 105 spores / ml. Inoculated samples have been fixed for histological observations. Results showed stomata penetration and pathogenic activity of fungus beginning from the third (3rd) to the fifth (5th) day after inoculation. We observed that conidia germination begins in less than 1 hour and reaches 95 to 100 % within 4 hours. The progress of the disease varies according to the according to the banana cultivars and the amount of conidia. 105 spores / ml have induced symptoms. Then, Deightoniella torulosa alone could induce symptoms and was involved in rapid necrosis of infected leaves in natural conditions. Keywords: Deightoniella torulosa, activité parasitaire, inoculations, cultivars de bananiers, sénescence, Côte-d\'Ivoire./Deightoniella torulosa, pathogenic activity, inoculations, cultivated banana, Côte-d\'Ivoire.Sciences & Nature Vol. 4 (2) 2007: pp. 179-18

Starch and oil in the donor cow diet and starch in substrate differently affect the in vitro ruminal biohydrogenation of linoleic and linolenic acids

Trans isomers of fatty acids exhibit different health properties. Among them, trans-10,cis-12 conjugated linoleic acid has negative effects on milk fat production and can affect human health. A shift from the trans-11 to the trans-10 pathway of biohydrogenation (BH) can occur in the rumen of dairy cows receiving high-concentrate diets, especially when the diet is supplemented with highly unsaturated fat sources. The differences of BH patterns between linoleic acid (LeA) and linolenic acid (LnA) in such ruminal conditions remain unknown; thus, the aim of this work was to investigate in vitro the effects of starch and sunflower oil in the diet of the donor cows and starch level in the incubates on the BH patterns and efficiencies of LeA and LnA. The design was a 4 × 4 Latin square design with 4 cows, 4 periods, and 4 diets with combinations of 21 or 34% starch and 0 or 5% sunflower oil. The rumen content of each cow during each period was incubated with 4 substrates, combining 2 starch levels and either LeA or LnA addition. Capillary electrophoresis single-strand conformation polymorphism of incubates showed that dietary starch decreased the diversity of the bacterial community and the high-starch plus oil diet modified its structure. High-starch diets poorly affected isomerization and first reduction of LeA and LnA, but decreased the efficiencies of trans-11,cis-15-C18:2 and trans C18:1 reduction. Dietary sunflower oil increased the efficiency of LeA isomerization but decreased the efficiency of trans C18:1 reduction. An interaction between dietary starch and dietary oil resulted in the highest trans-10 isomers production in incubates when the donor cow received the high-starch plus oil diet. The partition between trans-10 and trans-11 isomers was also affected by an interaction between starch level and the fatty acid added to the incubates, showing that the trans-10 shift only occurred with LeA, whereas LnA was mainly hydrogenated via the more usual trans-11 pathway, whatever the starch level in the substrate, although the bacterial communities were not different between LeA and LnA incubates. In LeA incubates, trans-10 isomer production was significantly related to the structure of the bacterial community

Altered splicing of the BIN1 muscle-specific exon in humans and dogs with highly progressive centronuclear myopathy

Amphiphysin 2, encoded by BIN1, is a key factor for membrane sensing and remodelling in different cell types. Homozygous BIN1 mutations in ubiquitously expressed exons are associated with autosomal recessive centronuclear myopathy (CNM), a mildly progressive muscle disorder typically showing abnormal nuclear centralization on biopsies. In addition, misregulation of BIN1 splicing partially accounts for the muscle defects in myotonic dystrophy (DM). However, the muscle-specific function of amphiphysin 2 and its pathogenicity in both muscle disorders are not well understood. In this study we identified and characterized the first mutation affecting the splicing of the muscle-specific BIN1 exon 11 in a consanguineous family with rapidly progressive and ultimately fatal centronuclear myopathy. In parallel, we discovered a mutation in the same BIN1 exon 11 acceptor splice site as the genetic cause of the canine Inherited Myopathy of Great Danes (IMGD). Analysis of RNA from patient muscle demonstrated complete skipping of exon 11 and BIN1 constructs without exon 11 were unable to promote membrane tubulation in differentiated myotubes. Comparative immunofluorescence and ultrastructural analyses of patient and canine biopsies revealed common structural defects, emphasizing the importance of amphiphysin 2 in membrane remodelling and maintenance of the skeletal muscle triad. Our data demonstrate that the alteration of the muscle-specific function of amphiphysin 2 is a common pathomechanism for centronuclear myopathy, myotonic dystrophy, and IMGD. The IMGD dog is the first faithful model for human BIN1-related CNM and represents a mammalian model available for preclinical trials of potential therapies