Vertical variation of atrazine mineralization capacity in soils

Se estudió la variación de la capacidad de mineralización de atrazina en dos suelos de la provincia de Córdoba, Argentina. Se seleccionaron dos perfiles de suelos con (suelo adaptado) y sin (suelo no adaptado) historia de aplicación de atrazina. La evolución de atrazina fue caracterizada mediante el balance de la radioactividad del 14C del anillo triazínico identificando las fracciones mineralizada,extractable y no extractable, durante incubaciones en condiciones de laboratorio con suelo tratado con calor (T105) y sin él (Control). La disminución de la capacidad de degradación de atrazina después de la inactivación microbiana y la limitada recuperación de esa capacidad observada en el perfil de suelo adaptado sugirió que los microorganismos degradadores de atrazina estarían distribuidos en todo el perfil siendo los responsables de la mineralización de atrazina en los horizontes subsuperficiales. Contrariamente, en el perfil de suelo no adaptado,la recuperación de la mineralización de atrazina a niveles similares o ligeramente superiores a los del Control corroboró la vía cometabólica del proceso característica de suelos sin historia de uso de atrazina. La capacidad de mineralizaciónde atrazina por los horizontes subsuperficiales, donde tiende a acumularse este herbicida, en suelos con aplicaciones frecuentes de atrazina debiera ser considerada y estimulada como un mecanismo de bioremediación natural

Valorisation de substrats organiques divers dans l'agriculture péri-urbaine de Ouagadougou (Burkina Faso) pour l'amendement et la fertilisation des sols : acteurs et pratiques

Organic substrates recycling in the sub-urban agriculture of Ouagadougou (Burkina Faso) for soils fertilization: description of the different actors and their practices. This study aimed to establish an overview of the main actors of the sub-urban agriculture of Ouagadougou, their practices and expectations, and to characterize the used organic substrates (OS). For that, a farm survey was carried out on the most representative sites with 64 persons (cereal farmers, truckers and nurserymen) randomly chosen. OS were sampled with each actor, when available. Thus, 27 samples were taken and characterized through chemical analyses and laboratory incubations to follow organic carbon mineralization. The results highlighted that 35% of cereal farmers, 69% of truckers and 95% of nurserymen were 20 to 40 years old, that a large proportion of cereal farmers and truckers were not sent to school while 70% of nurserymen had at least primary education. Sub-urban agriculture was the only activity for 43% of farmers, 83% of truckers and 91% of nurserymen. Moreover, 71% of cereal farmers and 73% of nurserymen used municipal wastes as source of organic matter against only 17% of truckers, the majority of them using animal manures. The main criterion of choice of OS was their availability without any other consideration. Even if a large majority of the actors think that composts of OS were better than brut OS, a minority of them used currently composts. The best ways for an adoption and utilization of OS composts were to facilitate their accessibility and to promote these composts, composting techniques and their interests through advertising in mass media. Chemical analyses and laboratory incubations showed a great variability of the SUW, both in the same group and actors' groups. However, in case of total organic carbon, total nitrogen and total phosphorous contents, the following tendencies were observed: Contenttruckers > Contentfarmers > Contentnurserymen. For the sum of major elements Ca, K, Mg, Na (SME), it was observed that SMEtruckers ≈ SMEfarmers > SMEnurserymen. In addition, except a few atypical substrates, OS used by nurserymen were largely stabilized compared to those of cereal farmers and truckers

A High-Throughput Screen Identifies a New Natural Product with Broad-Spectrum Antibacterial Activity

Due to the inexorable invasion of our hospitals and communities by drug-resistant bacteria, there is a pressing need for novel antibacterial agents. Here we report the development of a sensitive and robust but low-tech and inexpensive high-throughput metabolic screen for novel antibiotics. This screen is based on a colorimetric assay of pH that identifies inhibitors of bacterial sugar fermentation. After validation of the method, we screened over 39,000 crude extracts derived from organisms that grow in the diverse ecosystems of Costa Rica and identified 49 with reproducible antibacterial effects. An extract from an endophytic fungus was further characterized, and this led to the discovery of three novel natural products. One of these, which we named mirandamycin, has broad-spectrum antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, methicillin-resistant Staphylococcus aureus, and Mycobacterium tuberculosis. This demonstrates the power of simple high throughput screens for rapid identification of new antibacterial agents from environmental samples

Association of brain network dynamics with plasma biomarkers in subjective memory complainers

Using a single integrated analysis, we examined the relationship between brain networks and molecular pathways in a cohort of elderly individuals at risk for Alzheimer's disease. In 205 subjective memory complainers (124 females, mean age: 75.7 ± 3.4), individual functional connectome was computed for a total of 3081 functional connections (set A) and 6 core plasma biomarkers of Alzheimer's disease (set B) were assessed. Partial least squares correlation analysis identified one dimension of population covariation between the 2 sets (p < 0.006), which we named bioneural mode. Five core plasma biomarkers and 190 functional connections presented bootstrap ratios greater than the critical value |1.96|. T-tau protein showed a trend toward significance (bootstrap resampling = 1.64). The salience, the language, the visuospatial, and the default mode networks were the strongest significant networks. We detected a strong association between network dynamics and core pathophysiological blood biomarkers. Innovative composite biomarkers, such as the bioneural mode, are promising to provide outcomes and better inform drug development and clinical practice for neurodegenerative diseases

A Communal Bacterial Adhesin Anchors Biofilm and Bystander Cells to Surfaces

While the exopolysaccharide component of the biofilm matrix has been intensively studied, much less is known about matrix-associated proteins. To better understand the role of these proteins, we undertook a proteomic analysis of the V. cholerae biofilm matrix. Here we show that the two matrix-associated proteins, Bap1 and RbmA, perform distinct roles in the biofilm matrix. RbmA strengthens intercellular attachments. In contrast, Bap1 is concentrated on surfaces where it serves to anchor the biofilm and recruit cells not yet committed to the sessile lifestyle. This is the first example of a biofilm-derived, communally synthesized conditioning film that stabilizes the association of multilayer biofilms with a surface and facilitates recruitment of planktonic bystanders to the substratum. These studies define a novel paradigm for spatial and functional differentiation of proteins in the biofilm matrix and provide evidence for bacterial cooperation in maintenance and expansion of the multilayer biofilm

Urelumab alone or in combination with rituximab in patients with relapsed or refractory B-cell lymphoma

Altres ajuts: This study was supported by Bristol-Myers Squibb, Princeton, NJ.Urelumab, a fully human, non-ligand binding, CD137 agonist IgG4 monoclonal antibody, enhances T-cell and natural killer-cell antitumor activity in preclinical models, and may enhance cytotoxic activity of rituximab. Here we report results in patients with relapsed or refractory diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), and other B-cell lymphomas, in phase 1 studies evaluating urelumab alone (NCT01471210) or combined with rituximab (NCT01775631). Sixty patients received urelumab (0.3 mg/kg IV Q3W, 8 mg IV Q3W, or 8 mg IV Q6W); 46 received urelumab (0.1 mg/kg, 0.3 mg/kg, or 8 mg IV Q3W) plus rituximab 375 mg/m 2 IV QW. The maximum tolerated dose (MTD) of urelumab was determined to be 0.1 mg/kg or 8 mg Q3W after a single event of potential drug-induced liver injury occurred with urelumab 0.3 mg/kg. Treatment-related AEs were reported in 52% (urelumab: grade 3/4, 15%) and 72% (urelumab + rituximab: grade 3/4, 28%); three led to discontinuation (grade 3 increased AST, grade 4 acute hepatitis [urelumab]; one death from sepsis syndrome [urelumab plus rituximab]). Objective response rates/disease control rates were 6%/19% (DLBCL, n = 31), 12%/35% (FL, n = 17), and 17%/42% (other B-cell lymphomas, n = 12) with urelumab and 10%/24% (DLBCL, n = 29) and 35%/71% (FL, n = 17) with urelumab plus rituximab. Durable remissions in heavily pretreated patients were achieved; however, many were observed at doses exceeding the MTD. These data show that urelumab alone or in combination with rituximab demonstrated manageable safety in B-cell lymphoma, but the combination did not enhance clinical activity relative to rituximab alone or other current standard of care