Black-hole masses of type 1 AGN in the XMM-Newton bright serendipitous survey

We derive masses of the central super-massive black hole (SMBH) and accretion rates for 154 type1 AGN belonging to a well-defined X-ray-selected sample, the XMM-Newton Serendipitous Sample (XBS). To this end, we use the most recent "single-epoch" relations, based on Hbeta and MgII2798A emission lines, to derive the SMBH masses. We then use the bolometric luminosities, computed on the basis of an SED-fitting procedure, to calculate the accretion rates, both absolute and normalized to the Eddington luminosity (Eddington ratio). The selected AGNs cover a range of masses from 10^7 to 10^10 Msun with a peak around 8x10^8 Msun and a range of accretion rates from 0.01 to ~50 Msun/year (assuming an efficiency of 0.1), with a peak at ~1 Msun/year. The values of Eddington ratio range from 0.001 to ~0.5 and peak at 0.1.Comment: 14 pages, 11 figures. Accepted for publication in Astronomy & Astrophysic

SDSSJ143244.91+301435.3: a link between radio-loud narrow-line Seyfert 1 galaxies and compact steep-spectrum radio sources?

We present SDSSJ143244.91+301435.3, a new case of radio-loud narrow line Seyfert 1 (RL NLS1) with a relatively high radio power (P1.4GHz=2.1x10^25 W Hz^-1) and large radioloudness parameter (R1.4=600+/-100). The radio source is compact with a linear size below ~1.4 kpc but, contrary to most of the RL NLS1 discovered so far with such a high R1.4, its radio spectrum is very steep (alpha=0.93) and not supporting a 'blazar-like' nature. Both the small mass of the central super-massive black-hole and the high accretion rate relative to the Eddington limit estimated for this object (3.2x10^7 Msun and 0.27, respectively, with a formal error of ~0.4 dex on both quantities) are typical of the class of NLS1. Through a modeling of the spectral energy distribution of the source we have found that the galaxy hosting SDSSJ143244.91+301435.3 is undergoing a quite intense star-formation (SFR=50 Msun y^-1) which, however, is expected to contribute only marginally (~1 per cent) to the observed radio emission. The radio properties of SDSSJ143244.91+301435.3 are remarkably similar to those of compact steep spectrum (CSS) radio sources, a class of AGN mostly composed by young radio galaxies. This may suggest a direct link between these two classes of AGN, with the CSS sources possibly representing the misaligned version (the so-called parent population) of RL NLS1 showing blazar characteristics.Comment: 14 pages, 7 figures, 4 tables, accepted for publication in MNRA

Comparison between in vitro chemical and ex vivo biological assays to evaluate antioxidant capacity of botanical extracts

The anti-oxidative activity of plant-derived extracts is well-known and confers health-promoting effects on functional foods and food supplements. Aim of this work is to evaluate the capability of two different assays to predict the real biological antioxidant efficiency. At this purpose, extracts from five different plant-derived matrices and commercial purified phytochemicals were analyzed for their anti-oxidative properties by using well-standardized in vitro chemical method (TEAC) and an ex vivo biological assay. The biological assay, a cellular membrane system obtained from erythrocytes of healthy volunteers, is based on the capability of phytochemicals treatment to prevent membrane lipid peroxidation under oxidative stress by UV-B radiation. Plant extracts naturally rich in phenols with different structure and purified phytochemicals showed different in vitro and ex vivo antioxidant capacities. A high correlation between phenolic contents of the plant-derived extracts and their ability to prevent oxidative injuries in a biological system was found, thus underlying the relevance of this class of metabolites in preventing oxidative stress. On the other hand, a low correlation between the antioxidant capacities was shown between in vitro and ex vivo antioxidant assay. Moreover, data presented in this work show how food complex matrices are more effective in preventing oxidative damages at biological level than pure phytochemicals, even if for these latter, the antioxidant activity was generally higher than that observed for food complex matrices

Interaction of anticancer reduced Schiff base coumarin derivatives with human serum albumin investigated by fluorescence quenching and molecular modeling

The specific binding of five reduced Schiff base derived 7-amino-coumarin compounds with antitumor activity to human serum albumin, the principal binding protein of blood, was studied by fluorescence spectroscopy. Their conditional binding constants were computed and the reversible binding at the Sudlow’s site I was found to be strong (KD ~ 0.03-2.09 M). Based on the data albumin can provide a depot for the compounds and is responsible for their biodistribution and transport processes. The experimental data is complemented by protein– ligand docking calculations for two representatives which support the observations. The proton dissociation constants of the compounds were also determined by UV-Vis spectrophotometric and fluorometric titrations to obtain the actual charges and distribution of the species in the various protonation states at physiological pH

Quenched phosphorescence as alternative detection mode in the chiral separation of methotrexate by electrokinetic chromatography

Quenched phosphorescence was used, for the first time, as detection mode in the chiral separation of methotrexate (MTX) enantiomers by electrokinetic chromatography. The detection is based on dynamic quenching of the strong emission of the phosphorophore 1-bromo-4-naphthalene sulfonic acid (BrNS) by MTX under deoxygenated conditions. The use of a background electrolyte with 3 mg/mL 2-hydroxypropyl-β-cyclodextrin and 20% MeOH in 25 mM phosphate buffer (pH 7.0) and an applied voltage of 30 kV allowed the separation of l-MTX and its enantiomeric impurity d-MTX with sufficient resolution. In the presence of 1 mM BrNS, a detection limit of 3.2 × 10−7 M was achieved, about an order of magnitude better than published techniques based on UV absorption. The potential of the method was demonstrated with a degradation study and an enantiomeric purity assessment of l-MTX. Furthermore, l-MTX was determined in a cell culture extract as a proof-of-principle experiment to show the applicability of the method to biological samples

Erratum: Studying the relationship between X-ray emission and accretion in AGNs using theXMM–NewtonBright Serendipitous Survey

We study the link between the X-ray emission in radio-quiet active galactic nuclei (AGN) and the accretion rate on the central supermassive black hole using a statistically well-defined and representative sample of 71 type 1 AGN extracted from the XMM–Newton Bright Serendipitous Survey. We search and quantify the statistical correlations between some fundamental parameters that characterize the X-ray emission, i.e. the X-ray spectral slope, Γ, and the X-ray ‘loudness’, and the accretion rate, both absolute (Ṁ) and normalized to the Eddington luminosity (Eddington ratio, λ). We parametrize the X-ray loudness using three different quantities: the bolometric correction Kbol, the two-point spectral index αOX and the disc/corona luminosity ratio. We find that the X-ray spectral index depends on the normalized accretion rate while the ‘X-ray loudness’ depends on both the normalized and the absolute accretion rate. The dependence on the Eddington ratio, in particular, is probably induced by the Γ – λ correlation. The two proxies usually adopted in the literature to quantify the X-ray loudness of an AGN, i.e. Kbol and αOX, behave differently, with Kbol being more sensitive to the Eddington ratio and αOX having a stronger dependence with the absolute accretion. The explanation of this result is likely related to the different sensitivity of the two parameters to the X-ray spectral index

Characterization of the binding sites of the anticancer ruthenium(III) complexes KP1019 and KP1339 on human serum albumin via competition studies

Indazolium trans-[tetrachloridobis(1H-indazole)ruthenate(III)] (KP1019) and its Na+ analogue (KP1339) are two of the most prominent non-platinum antitumor metal complexes currently undergoing clinical trials. After intravenous administration, they are known to bind to human serum albumin (HSA) in a noncovalent manner. To elucidate their HSA binding sites, displacement reactions with the established site markers warfarin and dansylglycine as well as bilirubin were monitored by spectrofluorimetry, ultrafiltration-UV-vis spectrophotometry, and/or capillary zone electrophoresis. Conditional stability constants for the binding of KP1019 and KP1339 to sites I and II of HSA were determined, indicating that both Ru(III) compounds bind to both sites with moderately strong affinity (log K (1)' = 5.3-5.8). No preference for either binding site was found, and similar results were obtained for both metal complexes, demonstrating low influence of the counter ion on the binding event

Aloe barbadensis: how a miraculous plant becomes reality

Aloe barbadensis Miller is a plant that is native to North and East Africa and has accompanied man for over 5,000 years. The aloe vera plant has been endowed with digestive, dermatological, culinary and cosmetic virtues. On this basis, aloe provides a range of possibilities for fascinating studies from several points of view, including the analysis of chemical composition, the biochemistry involved in various activities and its application in pharmacology, as well as from horticultural and economic standpoints. The use of aloe vera as a medicinal plant is mentioned in numerous ancient texts such as the Bible. This multitude of medicinal uses has been described and discussed for centuries, thus transforming this miracle plant into reality. A summary of the historical uses, chemical composition and biological activities of this species is presented in this review. The latest clinical studies involved in vivo and in vitro assays conducted with aloe vera gel or its metabolites and the results of these studies are reviewed