Effects of buprenorphine on model development in an adjuvant-induced monoarthritis rat model

Complete Freund’s adjuvant (CFA)-induced arthritis in rats is a common animal model for studying chronic inflammatory pain. However, modelling of the disease is associated with unnecessary pain and impaired animal wellbeing, particularly in the immediate post-induction phase. Few attempts have been made to counteract these adverse effects with analgesics. The present study investigated the effect of buprenorphine on animal welfare, pain-related behaviour and model-specific parameters during the disease progression in a rat model of CFA-induced monoarthritis. The aim was to reduce or eliminate unnecessary pain in this model, in order to improve animal welfare and to avoid suffering, without compromising the quality of the model. Twenty-four male Sprague Dawley rats were injected with 20 μl of CFA into the left tibio-tarsal joint to induce monoarthritis. Rats were treated with either buprenorphine or carprofen for 15 days during the disease development, and were compared to a saline-treated CFA-injected group or a negative control group. Measurements of welfare, pain-related behaviour and clinical model-specific parameters were collected. The study was terminated after 3 weeks, ending with a histopathologic analysis. Regardless of treatment, CFA-injected rats displayed mechanical hyperalgesia and developed severe histopathological changes associated with arthritis. However, no severe effects on general welfare were found at any time. Buprenorphine treatment reduced facial pain expression scores, improved mobility, stance and lameness scores and it did not supress the CFA-induced ankle swelling, contrary to carprofen. Although buprenorphine failed to demonstrate a robust analgesic effect on the mechanical hyperalgesia in this study, it did not interfere with the development of the intended pathology

Canid vs. Canid: Insights into Coyote-Dog Encounters from Social Media

While the relationship between coyotes (Canis latrans) and house cats (Felis catus) may be characterized as one between predators and their prey, coyote interactions with domestic dogs (C. lupus familiaris) appear to be more varied and may include behaviors associated with canid sociality. While encounters between coyotes and dogs are difficult to observe, we capitalized on publically available video recordings of coyote-dog encounters to observe canid behaviors and examined 35 video clips downloaded from YouTube during fall 2014. We identified coyote-dog interactions that were playful, agonistic, or predatory; those that we could not clearly categorize were labeled as other/undetermined. We found that both species were recorded directing play to the other species, which led to mutual play bouts. We observed a similar number of agonistic encounters, which included dogs biting coyotes and coyotes biting dogs. The main difference in agonistic behavior was that coyotes usually showed defensive aggression while dogs did not show defensive aggression. We also observed coyotes ambushing and bite-shaking small dogs in 3 video clips, from which the dogs escaped, but we did not see predatory behavior of dogs towards coyotes. Dog size may be related to types of interactions. No small dogs were involved in agonistic interactions, and only 1 small dog was observed playing with a coyote. From these videos, we conclude that the relationship between coyotes and dogs cannot be simply described as predator-prey; indeed, much of it appears to be social behavior divided between playful and agonistic. Future work that aims to explain the proximate correlates of play and aggression would provide more information for managers who wish to educate humans to reduce wildlife-human-dog conflicts

Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (<i>Heterocephalus glaber</i>)

The present study investigated the cholinergic system in the African naked mole-rat (Heterocephalus glaber) with focus on the muscarinic acetylcholine receptor subtypes M(1) and M(4). The protein sequences for the subtypes m(1–5) of the naked mole-rat were compared to that of the house mouse (Mus musculus) using basic local alignment search tool (BLAST). The presence and function of M(1) and M(4) was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies with [(3)H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg/kg in the formalin test. Administration of 50 mg/kg VU0152100 resulted in a non-significant tendency towards antinociception. The antinociceptive effects were reversed by the muscarinic acetylcholine receptor antagonist atropine. Binding studies indicated presence of muscarinic acetylcholine receptors with a radioligand affinity comparable to that reported in mice. In conclusion, muscarinic acetylcholine receptor subtypes are present in the naked mole-rat and contribute to antinociception in the naked mole-rat

Unexpected short- and medium-range atomic structure of sputtered amorphous silicon upon thermal annealing

We investigate the structure of magnetron-sputtered (MS) amorphous silicon(a-Si) prepared under standard deposition conditions and compare this to pure ion-implanted (II) a-Si. The structure of both films is characterized in their as-prepared and thermally annealed states. Significant differences are observed in short- and medium-range order following thermal annealing. Whereas II a-Si undergoes structural relaxation toward a continuous random network, MS a-Si exhibits little change. Cross-sectional transmission electron microscopy reveals the presence of nanopores in the MS film consistent with reduced mass-density. Therefore, the short- and medium-range order of annealed, MS a-Si is tentatively attributed to these pores

DIAmante TESS AutoRegressive Planet Search (DTARPS): I. Analysis of 0.9 Million Light Curves

Nearly one million light curves from the TESS Year 1 southern hemisphere extracted from Full Frame Images with the DIAmante pipeline are processed through the AutoRegressive Planet Search statistical procedure. ARIMA models remove trends and lingering autocorrelated noise, the Transit Comb Filter identifies the strongest periodic signal in the light curve, and a Random Forest machine learning classifier is trained and applied to identify the best potential candidates. Classifier training sets include injections of both planetary transit signals and contaminating eclipsing binaries. The optimized classifier has a True Positive Rate of 92.8% and a False Positive Rate of 0.37% from the labeled training set. The result of this DIAmante TESS autoregressive planet search (DTARPS) analysis is a list of 7,377 potential exoplanet candidates. The classifier has a False Positive Rate of 0.3%, a 64% recall rate for previously confirmed exoplanets, and a 78% negative recall rate for known False Positives. The completeness map of the injected planetary signals shows high recall rates for planets with 8 - 30 R(Earth) radii and periods 0.6-13 days and poor completeness for planets with radii < 2 R(Earth) or periods < 1 day. The list has many False Alarms and False Positives that need to be culled with multifaceted vetting operations (Paper II).Comment: 46 pages, 21 figures, submitted to AAS Journals. A Machine Readable Table for Table 3 is available at https://drive.google.com/drive/folders/1DyxNcNlfcHHAoCdsaipxxIbP5A2FPey

DIAmante TESS AutoRegressive Planet Search (DTARPS): II. Hundreds of New TESS Candidate Exoplanets

The DIAmante TESS AutoRegressive Planet Search (DTARPS) project seeks to identify photometric transiting planets from 976,814 southern hemisphere stars observed in Year 1 of the TESS mission. This paper follows the methodology developed by Melton et al. (Paper I) using light curves extracted and pre-processed by the DIAmante project (Montalto et al. 2020). Paper I emerged with a list of 7,377 light curves with statistical properties characteristic of transiting planets but dominated by False Alarms and False Positives. Here a multistage vetting procedure is applied including: centroid motion and crowding metrics, False Alarm and False Positive reduction, photometric binary elimination, and ephemeris match removal. The vetting produces a catalog of 462 DTARPS Candidates across the southern ecliptic hemisphere and 310 objects in a spatially incomplete Galactic Plane list. Fifty-eight percent were not previously identified as transiting systems. Candidates are flagged for possible blending from nearby stars based on Zwicky Transient Facility data and for possible radial velocity variations based on Gaia satellite data. Orbital periods and planetary radii are refined using astrophysical modeling; the resulting parameters closely match published values for Confirmed Planets. Their properties are discussed in Paper III.Comment: 25 pages, 10 figures, submitted to AAS Journals. Machine Readable Tables and Figure Sets for Tables 1 and 4 are available at https://drive.google.com/drive/folders/1DyxNcNlfcHHAoCdsaipxxIbP5A2FPeyi?usp=share_lin

DIAmante TESS AutoRegressive Planet Search (DTARPS): III. Understanding the DTARPS Candidate Transiting Planet Catalogs

The DIAmante TESS AutoRegressive Planet Search (DTARPS) project, using novel statistical methods, has identified several hundred candidates for transiting planetary systems obtained from 0.9 million Full Frame Image light curves obtained in the TESS Year 1 southern hemisphere survey (Melton et al. 2022a and 2022b). Several lines of evidence, including limited reconnaissance spectroscopy, indicate that at least half are true planets rather than False Positives. Here various population properties of these objects are examined. Half of the DTARPS candidates are hot Neptunes, populating the 'Neptune desert' found in Kepler planet samples. The DTARPS samples also identify dozens of Ultra Short Period planets with orbital periods down to 5 hours, high priority systems for atmospheric transimssion spectroscopy, and planets orbiting low-mass M stars. DTARPS methodology is sufficiently well-characterized at each step that preliminary planet occurrence rates can be estimated. Except for the increase in hot Neptunes, DTARPS planet occurrence rates are consistent with Kepler rates. Overall, DTARPS provides one of the largest and most reliable catalog of TESS exoplanet candidates that can be tapped to improve our understanding of various exoplanetary populations and astrophysical processes.Comment: 29 pages, 16 figures, submitted to the AAS Journals February 13, 202