Deletion of the S3–S4 Linker in theShaker Potassium Channel Reveals Two Quenching Groups near the outside of S4

When attached outside the voltage-sensing S4 segment of the Shaker potassium channel, the fluorescent probe tetramethylrhodamine (TMRM) undergoes voltage-dependent fluorescence changes (ΔF) due to differential interaction with a pH-titratable external protein-lined vestibule (Cha, A., and F. Bezanilla. 1998. J. Gen. Physiol. 112:391–408.). We attached TMRM at the same sites [corresponding to M356C and A359C in the wild-type (wt) channel] in a deletion mutant of Shaker where all but the five amino acids closest to S4 had been removed from the S3–S4 linker. In the deletion mutant, the maximal ΔF/F seen was diminished 10-fold, and the ΔF at M356C became pH independent, suggesting that the protein-lined vestibule is made up in large part by the S3–S4 linker. The residual ΔF showed that the probe still interacted with two putative quenching groups near the S4 segment. One group was detected by M356C-TMRM (located outside of S3 in the deletion mutant) and reported on deactivation gating charge movement when applying hyperpolarizing voltage steps from a holding potential of 0 mV. During activating voltage steps from a holding potential of −90 mV, the fluorescence lagged considerably behind the movement of gating charge over a range of potentials. Another putative quenching group was seen by probes attached closer to the S4 and caused a ΔF at extreme hyperpolarizations (more negative than −90 mV) only. A signal from the interaction with this group in the wt S3–S4 linker channel (at L361C) correlated with gating charge moving in the hyperpolarized part of the Q-V curve. Probe attached at A359C in the deletion mutant and at L361C in wt channel showed a biphasic ΔF as the probe oscillated between the two groups, revealing that there is a transient state of the voltage sensor in between, where the probe has maximal fluorescence. We conclude that the voltage sensor undergoes two distinct conformational changes as seen from probes attached outside the S4 segment

Quantum Chaos Border for Quantum Computing

We study a generic model of quantum computer, composed of many qubits coupled by short-range interaction. Above a critical interqubit coupling strength, quantum chaos sets in, leading to quantum ergodicity of the computer eigenstates. In this regime the noninteracting qubit structure disappears, the eigenstates become complex and the operability of the computer is destroyed. Despite the fact that the spacing between multi-qubit states drops exponentially with the number of qubits $n$, we show that the quantum chaos border decreases only linearly with $n$. This opens a broad parameter region where the efficient operation of a quantum computer remains possible.Comment: revtex, 4 pages, 5 figures, more details and data adde

Dispersive Manipulation of Paired Superconducting Qubits

We combine the ideas of qubit encoding and dispersive dynamics to enable robust and easy quantum information processing (QIP) on paired superconducting charge boxes sharing a common bias lead. We establish a decoherence free subspace on these and introduce universal gates by dispersive interaction with a LC resonator and inductive couplings between the encoded qubits. These gates preserve the code space and only require the established local symmetry and the control of the voltage bias.Comment: 5 pages, incl. 1 figur

Combustion of fuel blends containing digestate pyrolysis oil in a multi-cylinder compression ignition engine

Digestate from the anaerobic digestion conversion process is widely used as a farm land fertiliser. This study proposes an alternative use as a source of energy. Dried digestate was pyrolysed and the resulting oil was blended with waste cooking oil and butanol (10, 20 and 30 vol.%). The physical and chemical properties of the pyrolysis oil blends were measured and compared with pure fossil diesel and waste cooking oil. The blends were tested in a multi-cylinder indirect injection compression ignition engine.Engine combustion, exhaust gas emissions and performance parameters were measured and compared with pure fossil diesel operation. The ASTM copper corrosion values for 20% and 30% pyrolysis blends were 2c, compared to 1b for fossil diesel. The kinematic viscosities of the blends at 40 C were 5–7 times higher than that of fossil diesel. Digested pyrolysis oil blends produced lower in-cylinder peak pressures than fossil diesel and waste cooking oil operation. The maximum heat release rates of the blends were approximately 8% higher than with fossil diesel. The ignition delay periods of the blends were higher; pyrolysis oil blends started to combust late and once combustion started burnt quicker than fossil diesel. The total burning duration of the 20% and 30% blends were decreased by 12% and 3% compared to fossil diesel. At full engine load, the brake thermal efficiencies of the blends were decreased by about 3–7% when compared to fossil diesel. The pyrolysis blends gave lower smoke levels; at full engine load, smoke level of the 20% blend was 44% lower than fossil diesel. In comparison to fossil diesel and at full load, the brake specific fuel consumption (wt.) of the 30% and 20% blends were approximately 32% and 15% higher. At full engine load, the CO emission of the 20% and 30% blends were decreased by 39% and 66% with respect to the fossil diesel. Blends CO2 emissions were similar to that of fossil diesel; at full engine load, 30% blend produced approximately 5% higher CO2 emission than fossil diesel. The study concludes that on the basis of short term engine experiment up to 30% blend of pyrolysis oil from digestate of arable crops can be used in a compression ignition engine

The spectral gap for some spin chains with discrete symmetry breaking

We prove that for any finite set of generalized valence bond solid (GVBS) states of a quantum spin chain there exists a translation invariant finite-range Hamiltonian for which this set is the set of ground states. This result implies that there are GVBS models with arbitrary broken discrete symmetries that are described as combinations of lattice translations, lattice reflections, and local unitary or anti-unitary transformations. We also show that all GVBS models that satisfy some natural conditions have a spectral gap. The existence of a spectral gap is obtained by applying a simple and quite general strategy for proving lower bounds on the spectral gap of the generator of a classical or quantum spin dynamics. This general scheme is interesting in its own right and therefore, although the basic idea is not new, we present it in a system-independent setting. The results are illustrated with an number of examples.Comment: 48 pages, Plain TeX, BN26/Oct/9

Various correlations in a Heisenberg XXZ spin chain both in thermal equilibrium and under the intrinsic decoherence

In this paper we discuss various correlations measured by the concurrence (C), classical correlation (CC), quantum discord (QD), and geometric measure of discord (GMD) in a two-qubit Heisenberg XXZ spin chain in the presence of external magnetic field and Dzyaloshinskii-Moriya (DM) anisotropic antisymmetric interaction. Based on the analytically derived expressions for the correlations for the cases of thermal equilibrium and the inclusion of intrinsic decoherence, we discuss and compare the effects of various system parameters on the correlations in different cases. The results show that the anisotropy Jz is considerably crucial for the correlations in thermal equilibrium at zero temperature limit but ineffective under the consideration of the intrinsic decoherence, and these quantities decrease as temperature T rises on the whole. Besides, J turned out to be constructive, but B be detrimental in the manipulation and control of various quantities both in thermal equilibrium and under the intrinsic decoherence which can be avoided by tuning other system parameters, while D is constructive in thermal equilibrium, but destructive in the case of intrinsic decoherence in general. In addition, for the initial state $|\Psi_1(0) > = \frac{1}{\sqrt{2}} (|01 > + |10 >)$, all the correlations except the CC, exhibit a damping oscillation to a stable value larger than zero following the time, while for the initial state $|\Psi_2(0) > = \frac{1}{\sqrt{2}} (|00 > + |11 >)$, all the correlations monotonously decrease, but CC still remains maximum. Moreover, there is not a definite ordering of these quantities in thermal equilibrium, whereas there is a descending order of the CC, C, GMD and QD under the intrinsic decoherence with a nonnull B when the initial state is $|\Psi_2(0) >$.Comment: 8 pages, 7 figure

Genome-wide analyses identify a role for SLC17A4 and AADAT in thyroid hormone regulation.

Thyroid dysfunction is an important public health problem, which affects 10% of the general population and increases the risk of cardiovascular morbidity and mortality. Many aspects of thyroid hormone regulation have only partly been elucidated, including its transport, metabolism, and genetic determinants. Here we report a large meta-analysis of genome-wide association studies for thyroid function and dysfunction, testing 8 million genetic variants in up to 72,167 individuals. One-hundred-and-nine independent genetic variants are associated with these traits. A genetic risk score, calculated to assess their combined effects on clinical end points, shows significant associations with increased risk of both overt (Graves' disease) and subclinical thyroid disease, as well as clinical complications. By functional follow-up on selected signals, we identify a novel thyroid hormone transporter (SLC17A4) and a metabolizing enzyme (AADAT). Together, these results provide new knowledge about thyroid hormone physiology and disease, opening new possibilities for therapeutic targets