The Behaviour of Both Listeria monocytogenes and Rat Ciliated Ependymal Cells Is Altered during Their Co-Culture

Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis

Kajian Normatif: Pengelolaan Perbataan dan Kelembagaan Pusat-daerah Berdasarkan Rencana Induk Pengelolaan Perbatasan Negara

Penulis ingin menelaah bagaimana rentang kendali kelembagaan dari pusat ke daerah dalam mengelolawilayah perbatasan berdasarkan rencana induk pengelolaan perbatasan negara. Rencana induk pengelolaanbatas wilayah negara dan kawasan perbatasan atau Rinduk adalah rencana pembangunan nasional jangkamenengah 5 (lima) tahun yang memberikan arah kebijakan, strategi, dan program pengelolaan batas wilayahnegara dan pembangunan kawasan perbatasan yang berpedoman pada Rencana Pembangunan Jangka PanjangNasional atau RPJPN dan Rencana Pembangunan Jangka Menengah Nasional atau RPJMN. Metode penelitianpada paper ini menggunakan metode normatif, dimana secara kualitatif ditelaah berbagai regulasi yang terkaitdengan lembaga dan kelembagaan pengelola perbatasan Negara Kesatuan Republik Indonesia. Regulasimengenai kelembagaan (Badan Nasional Pengelola Perbatasan (BNPP) yang mengatur tata kelola wilayahperbatasan negara sudah ada, namun perlu penguatan kelembagaan yang lebih lagi, pelatihan sumber dayamanusia dan penyediaan dana yang dibutuhkan sesuai dengan prioritas penanganan perbatasan Negara

Pemberian Motivasi Cinta Lingkungan Kepada Siswasiswi SMA

Kerusakan kualitas lingkungan disebabkan juga oleh sistem pendidikan yang tidak memperhatikanpendidikan lingkungan terhadap anak didik sejak dini. Tujuan pengabdian kepada masyarakat ini adalahmemberikan motivasi kepada siswa-siswa SMA dalam memelihara lingkungan sejak dini. Metodologi penulisanlaporan ini adalah dengan metode deskriptif. Metode pelaksanaan diawali dengan rapat perencanaan, persiapanbahan, pelaksanaan dan evaluasi. Kegiatan pelaksanaan dilakukan dengan mengumpulkan siswa-siswi SMA dariPerguruan Advent Bogor dalam suatu kelas, lalu diberikan paparan mengapa mereka perlu mencintailingkungan demi generasi mereka sendiri. Perhatian utama yang menjadi acuan adalah lingkungan sungai,karena semua sampah pada akhirnya akan hanyut ke aliran sungai menuju laut. Pemaparan juga mengenaiSungai Citarum yang menjadi perhatian dunia karena sangat kotor dan sampai saat ini belum dapat dibersihkansecara baik. Para peserta, siswa-siswi dan guru-guru, memperhatikan dengan seksama dan banyak bertanyamengenai siapa yang bertanggung jawab mengenai kelestarian lingkungan apakah pemesrintah atau pihaklainnya. Pihak yang terutama menjaga lingkungan adalah diri sendiri agar lingkungan yang bersih dapatdinikmati secara baik sampai dengan generasi yang akan datang

A ~4.6 h quasi-periodic oscillation in the BL Lacertae PKS 2155-304?

We report a possible detection of an ~4.6-hour quasi-periodic oscillation (QPO) in the 0.3-10 keV emission of the high-energy peaked blazar PKS 2155-304 from a 64 ks observation by the XMM-Newton EPIC/pn detector. We identify a total modulation of ~5% in the light curve and confirm that nominal period by periodogram, structure function and wavelet analyses. The limited light curve duration allows the capture of only 3.8 cycles of this oscillation and thus precludes a very strong claim for this QPO, despite a nominally high (>3 sigma) statistical significance. We briefly discuss models capable of producing an X-ray QPO of such a period in a blazar.Comment: 4 pages, 6 figures, accepted for publication in A&A Letter

Hydrocephalus and diffuse choroid plexus hyperplasia in primary ciliary dyskinesia-related MCIDAS mutation

OBJECTIVE: To report a neuroradiologic phenotype associated with reduced generation of multiple motile cilia (RGMC) and mutations in the multicilin gene. We hypothesize that the observed phenotype may reflect the emerging role that ependymal cilia play in regulating CSF production. METHOD: Clinical and radiologic records were retrospectively reviewed for 7 consecutive patients diagnosed by the Leicester UK national primary ciliary dyskinesia (PCD) diagnostic laboratory. RESULTS: On MRI scanning, all patients demonstrated hydrocephalus, choroid plexus hyperplasia (CPH), and arachnoid cysts. No patient had any sign of neurologic deficit. All patients had significant lung disease. CONCLUSIONS: We conclude that there is a high incidence of hydrocephalus, arachnoid cysts, and CPH in MCIDAS-associated RGMC. In all cases, the observed hydrocephalus seems arrested in childhood without progression or adverse neurologic sequelae. Our new observation of CPH, which is associated with CSF overproduction, is the first macroscopic evidence that ependymal cilia may be involved in the regulation of CSF production and flow. We suggest that brain imaging should be performed in all cases of RGMC and that a diagnosis of PCD or RGMC be strongly considered in patients with unexplained hydrocephalus and a lifelong “wet”-sounding cough

Hydrocephalus and diffuse choroid plexus hyperplasia in primary ciliary dyskinesia-related MCIDAS mutation

Objective: To report a neuroradiologic phenotype associated with reduced generation of multiple motile cilia (RGMC) and mutations in the multicilin gene. We hypothesize that the observed phenotype may reflect the emerging role that ependymal cilia play in regulating CSF production. Method: Clinical and radiologic records were retrospectively reviewed for 7 consecutive patients diagnosed by the Leicester UK national primary ciliary dyskinesia (PCD) diagnostic laboratory. Results: On MRI scanning, all patients demonstrated hydrocephalus, choroid plexus hyperplasia (CPH), and arachnoid cysts. No patient had any sign of neurologic deficit. All patients had significant lung disease. Conclusions: We conclude that there is a high incidence of hydrocephalus, arachnoid cysts, and CPH in MCIDAS-associated RGMC. In all cases, the observed hydrocephalus seems arrested in childhood without progression or adverse neurologic sequelae. Our new observation of CPH, which is associated with CSF overproduction, is the first macroscopic evidence that ependymal cilia may be involved in the regulation of CSF production and flow. We suggest that brain imaging should be performed in all cases of RGMC and that a diagnosis of PCD or RGMC be strongly considered in patients with unexplained hydrocephalus and a lifelong “wet”-sounding cough

Long-time Low-latency Quantum Memory by Dynamical Decoupling

Quantum memory is a central component for quantum information processing devices, and will be required to provide high-fidelity storage of arbitrary states, long storage times and small access latencies. Despite growing interest in applying physical-layer error-suppression strategies to boost fidelities, it has not previously been possible to meet such competing demands with a single approach. Here we use an experimentally validated theoretical framework to identify periodic repetition of a high-order dynamical decoupling sequence as a systematic strategy to meet these challenges. We provide analytic bounds-validated by numerical calculations-on the characteristics of the relevant control sequences and show that a "stroboscopic saturation" of coherence, or coherence plateau, can be engineered, even in the presence of experimental imperfection. This permits high-fidelity storage for times that can be exceptionally long, meaning that our device-independent results should prove instrumental in producing practically useful quantum technologies.Comment: abstract and authors list fixe

Ciliary dyskinesia is an early feature of respiratory syncytial virus infection

Respiratory syncytial virus is a major cause of respiratory disease. There are conflicting accounts of the response of human epithelial cells to respiratory syncytial virus and a lack of data on its effect on ciliary function. Our aim was to study the early stages of respiratory syncytial virus infection of primary human basal and ciliated cultures. Using high speed videomicroscopy, we found that ciliary beat frequency was unaffected by respiratory syncytial virus infection over 72 h; however, ciliary dyskinesia significantly increased within 24 h of infection (p<0.05). Transmission electron microscopy revealed that ultrastructural abnormalities were confined to ciliated cells, including increased cilia loss and mitochondrial damage. Confocal immunofluorescence microscopy showed that respiratory syncytial virus antigen gradually spread from the cell surface to the ciliary tip of infected cells over 3 days. Interestingly, ciliated cultures secreted fewer viruses than basal (progenitor) cell cultures and produced a chemokine response focused on recruitment of neutrophils. This study highlights differences in infection models and underscores the need to explore further the role of ciliated cells in the establishment of respiratory syncytial virus infection. Increased ciliary dyskinesia combined with ciliary loss and epithelial damage is likely to result in reduced mucociliary clearance early in the infective process

Ciliary central microtubular orientation is of no clinical significance in bronchiectasis

It has been suggested that patients with bronchiectasis might have increased central microtubular orientation angle (CMOA), which leads to poor coordination of ciliary beating, and consequently impairment of airway defence. We have employed transmission electron microscopy to assess CMOA of ciliated nasal mucosa in a cohort of 133 (81F, 56.8±16.1 yr) stable bronchiectasis and 59 healthy subjects (30F, 49.3±22.1 yr). There was no significant difference in CMOA between bronchiectasis (13.2 degree) and control subjects (13.0 degree, P = 0.82). There was no significant difference in CMOA among patients according to the etiology of bronchiectasis, presence of nasal symptoms, or sputum status of Pseudomonas aeruginosa infection. Patients with more severe bronchiectasis, i.e. those with FEV 1 0.05). CMOA correlated with ciliary beat frequency (negative), and the percent of cilia showing ultrastructural or microtubular defects (P < 0.05). Central microtubular orientation angle does not correlate with clinically important parameters, in contrary to the results reported by previously published smaller scale studies. © 2004 Elsevier Ltd. All rights reserved.postprin

Risk factors for situs defects and congenital heart disease in primary ciliary dyskinesia

Primary ciliary dyskinesia (PCD) is associated with abnormal organ positioning (situs) and congenital heart disease (CHD). This study investigated genotype–phenotype associations in PCD to facilitate risk predictions for cardiac and laterality defects. This retrospective cohort study of 389 UK patients with PCD found 51% had abnormal situs and 25% had CHD and/or laterality defects other than situs inversus totalis. Patients with biallelic mutations in a subset of nine PCD genes had normal situs. Patients with consanguineous parents had higher odds of situs abnormalities than patients with non-consanguineous parents. Patients with abnormal situs had higher odds of CHD and/or laterality defects