Tourmaline 40Ar/39Ar chronology of tourmaline-rich rocks from Central Iberia dates the main Variscan deformation phases

During crustal thickening, metapelites taken to depth release boron-bearing hydrothermal fluids because of progressive heating and dehydration. These fluids swiftly percolate upwards, especially if the crust is being actively deformed, to form tourmaline where the PT conditions and the chemical composition of the host-rock are favorable. The age of the so-formed tourmaline would record the age of the upward admittance of B-bearing fluids and, presumably, the age of the deformation. This process has been documented in the Martinamor Antiform of Central Iberia, a region where tourmaline-bearing rocks are particularly abundant. Metasomatic tourmaline from the Late Cambrian San Pelayo orthogneisses (zircon U-Pb age of 496 ± 5 Ma) yielded 40Ar/39Ar plateau ages at 370 ± 5 Ma and 342 ± 5 Ma. The first value represents the crystallization age of the tourmaline and is so far the most precise estimation of the age of crustal thickening in Central Iberia (D1). The second value reflects a partial loss of Ar caused by the second deformation phase (D2). Tourmaline from mylonitized and folded tourmalinites developed above D2 shear zones yield perturbed spectra with mean "plateau" ages of 347 ± 9 Ma and 342 ± 9 Ma which may represent either the resetting of older tourmaline or the formation of new tourmaline by focused boron metasomatism. After the metamorphic peak and simultaneously with the emplacement of the main granitoids of the Avila Batholith (310-315 Ma), another episode of boron metasomatism precipitated a new generation of tourmaline, which appears either concentrated in fine-layered tourmalinites (318 ± 2 Ma) or disseminated within Ediacaran-Cambrian metasediments (316 ± 2 Ma). The source of boron was the breakdown of previously formed tourmaline during melting reactions. Lastly, tourmaline from a leucogranitic body yielded a saddle-shaped age spectrum with a minimum age of ca. 296 Ma, roughly coeval with the youngest leucograni - tes. Although further work is required, our results suggest that tourmaline can be a useful chronological marker for dating deformation and magmatism

Multivariate manifold-valued curve regression in time

Fr\'echet global regression is extended to the context of bivariate curve stochastic processes with values in a Riemannian manifold. The proposed regression predictor arises as a reformulation of the standard least-squares parametric linear predictor in terms of a weighted Fr\'echet functional mean. Specifically, in our context, in this reformulation, the Euclidean distance is replaced by the integrated quadratic geodesic distance. The regression predictor is then obtained from the weighted Fr\'echet curve mean, lying in the time-varying geodesic submanifold, generated by the regressor process components involved in the time correlation range. The regularized Fr\'echet weights are computed in the time-varying tangent spaces. The uniform weak-consistency of the regression predictor is proved. Model selection is also addressed. A simulation study is undertaken to illustrate the performance of the spherical curve variable selection algorithm proposed in a multivariate framework.Comment: 24 pages, 8 Figure

Mitochondria and the NLRP3 Inflammasome in Alcoholic and Nonalcoholic Steatohepatitis

Alcoholic (ASH) and nonalcoholic steatohepatitis (NASH) are advanced stages of fatty liver disease and two of the most prevalent forms of chronic liver disease. ASH and NASH are associated with significant risk of further progression to cirrhosis and hepatocellular carcinoma (HCC), the most common type of liver cancer, and a major cause of cancer-related mortality. Despite extensive research and progress in the last decades to elucidate the mechanisms of the development of ASH and NASH, the pathogenesis of both diseases is still poorly understood. Mitochondrial damage and activation of inflammasome complexes have a role in inducing and sustaining liver damage. Mitochondrial dysfunction produces inflammatory factors that activate the inflammasome complexes. NLRP3 inflammasome (nucleotide-binding oligomerization domain-like receptor protein 3) is a multiprotein complex that activates caspase 1 and the release of pro-inflammatory cytokines, including interleukin-1? (IL-1?) and interleukin-18 (IL-18), and contributes to inflammatory pyroptotic cell death. The present review, which is part of the issue "Mitochondria in Liver Pathobiology", provides an overview of the role of mitochondrial dysfunction and NLRP3 activation in ASH and NASH

Performance of malolactic fermentation by inoculation of selected Lactobacillus plantarum and Oenococcus oeni strains isolated from Rioja red wines

Malolactic fermentations (MLF) of wines inoculated with selected lactic acid bacteria strains of the species Oenococcus oeni and Lactobacillus plantarum were studied and compared with spontaneous MLF. Bacterial populations were monitored along the whole process of MLF and bacteria identifications were carried out at species and strain level. Macrorestriction analysis with SfiI endonuclease and subsequent PFGE was carried out in order to identify O. oeni individual strains. L. plantarum active lyophila did not survive competing with the indigenous microbiota in a wine with 15.3 % (vol/vol) alcohol, whereas the selected O. oeni strains carried out wine MLF. The highest production of histamine took place during MLF in those wines that underwent spontaneous MLF with a mixed population of indigenous strains. The lowest levels of histamine were obtained with the selected commercial O. oeni strain that succeeded 100 % over the indigenous microbiota. Results indicate that development of MLF leaded by selected O. oeni active lyophila provides negligible histamine levels in red wines of quality that can be submitted to subsequent ageing in wood.

Complex Carbohydrates in the Cyst Wall of Histriculus similis

The ultrastructure of the cyst wall has been studied in severa! hypotrichous ciliates. In Oxytrichafallax (Grimes, 1973),Pleurotricha sp. (Matsusaka, 1976), Stylonychia mytilus (Walker et al., 1975), Gastrostyla steinii (Walker et al., 1980) and Laurentiella acuminata (Gutierrez et al., l 980), the cyst wall is composed of four layers called, from outside to inside :the ectocyst, meso­ cyst, endocyst and granular layers. In these ciliates a resorption of cilia and kinetosomes occurs during encystment, and the mature cyst is termed kinetosome resorbing. Diophrys scutum (Walker & Maugel , 1980) has a three layered cyst wall and the mature cyst is non kinetosome resorbing. In the present work a structural and cytochemical study of the cyst wall in Histriculus similis was carried out by means of light microscopy and transmission electron microscopy

Neutron radiobiology studies with a pure cold neutron beam

Data on the radiobiological effects of thermal neutrons are usually obtained from irradiations in a mixed field of neutrons of different energies and gamma rays or from conversion of proton data with similar energies to those created in the neutron capture on nitrogen. Experimental data from irradiations in a pure thermal or cold neutron beam can help to find new values for neutron relative biological effectiveness (RBE) factors, which are useful for BNCT (Boron Neutron Capture Therapy) and radiation protection applications. We present a new experimental setup for radiobiological studies at a cold neutron beam at Institut Laue-Langevin, a beam without fast neutron component and almost no gamma ray contribution. After the irradiation, survival assays are performed to obtain the survival curves. Finally, comparing with a reference photon irradiation, the thermal neutron RBE factors can be calculated. The methodology is outlined at the example of A375 melanoma cells for which new radiobiological data were obtained.We acknowledge financial support for this work from the Fundación Científica de la Asociación Española Contra el Cáncer (AECC) under grant PS16163811PORR, Junta de Andalucía (Andalusian Regional Government), under contract P11-FQM-8229, Spanish MINECO and FEDER funds under contract FIS2015-69941-C2-1-P, the grant agreement ILL-UGR and the founders of the University of Granada Chair Neutrons for Medicine: Spanish Fundación ACS and Capitán Antonio. M.P. acknowledges a grant under the program Becas de Iniciación a la Investigación from the Universidad de Granada (Plan Propio de Investigación). The open access fee was covered by FILL2030, a European Union project within the European Commission’s Horizon 2020 Research and Innovation programme under grant agreement N°731096