110 research outputs found
Herschel SPIRE-FTS Observations of Excited CO and [CI] in the Antennae (NGC 4038/39): Warm and Cold Molecular Gas
We present Herschel SPIRE-FTS observations of the Antennae (NGC 4038/39), a
well studied, nearby ( Mpc) ongoing merger between two gas rich spiral
galaxies. We detect 5 CO transitions ( to ), both [CI]
transitions and the [NII] transition across the entire system, which
we supplement with ground based observations of the CO , and
transitions, and Herschel PACS observations of [CII] and [OI].
Using the CO and [CI] transitions, we perform both a LTE analysis of [CI], and
a non-LTE radiative transfer analysis of CO and [CI] using the radiative
transfer code RADEX along with a Bayesian likelihood analysis. We find that
there are two components to the molecular gas: a cold ( K)
and a warm ( K) component. By comparing the warm gas mass
to previously observed values, we determine a CO abundance in the warm gas of
. If the CO abundance is the same in the warm and
cold gas phases, this abundance corresponds to a CO luminosity-to-mass
conversion factor of $\alpha_{CO} \sim 7 \ M_{\odot}{pc^{-2} \ (K \ km \
s^{-1})^{-1}}_263\mu m\sim 0.01 L_{\odot}/M_{\odot}G_0\sim 1000$. Finally, we find
that a combination of turbulent heating, due to the ongoing merger, and
supernova and stellar winds are sufficient to heat the molecular gas.Comment: 50 pages, 15 figures, 8 tables, Accepted for publication in The
Astrophysical Journa
The GALEX Arecibo SDSS Survey. I. Gas Fraction Scaling Relations of Massive Galaxies and First Data Release
We introduce the GALEX Arecibo SDSS Survey (GASS), an on-going large program
that is gathering high quality HI-line spectra using the Arecibo radio
telescope for an unbiased sample of ~1000 galaxies with stellar masses greater
than 10^10 Msun and redshifts 0.025<z<0.05, selected from the SDSS
spectroscopic and GALEX imaging surveys. The galaxies are observed until
detected or until a low gas mass fraction limit (1.5-5%) is reached. This paper
presents the first Data Release, consisting of ~20% of the final GASS sample.
We use this data set to explore the main scaling relations of HI gas fraction
with galaxy structure and NUV-r colour. A large fraction (~60%) of the galaxies
in our sample are detected in HI. We find that the atomic gas fraction
decreases strongly with stellar mass, stellar surface mass density and NUV-r
colour, but is only weakly correlated with galaxy bulge-to-disk ratio (as
measured by the concentration index of the r-band light). We also find that the
fraction of galaxies with significant (more than a few percent) HI decreases
sharply above a characteristic stellar surface mass density of 10^8.5 Msun
kpc^-2. The fraction of gas-rich galaxies decreases much more smoothly with
stellar mass. One of the key goals of GASS is to identify and quantify the
incidence of galaxies that are transitioning between the blue, star-forming
cloud and the red sequence of passively-evolving galaxies. Likely transition
candidates can be identified as outliers from the mean scaling relations
between gas fraction and other galaxy properties. [abridged]Comment: 25 pages, 12 figures. Accepted for publication in MNRAS. Version with
high resolution figures available at
http://www.mpa-garching.mpg.de/GASS/pubs.ph
Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat 3 wild emmer wheat RIL population
Mineral nutrient malnutrition, and particularly
deficiency in zinc and iron, afflicts over 3 billion people
worldwide. Wild emmer wheat, Triticum turgidum ssp.
dicoccoides, genepool harbors a rich allelic repertoire for
mineral nutrients in the grain. The genetic and physiological
basis of grain protein, micronutrients (zinc, iron,
copper and manganese) and macronutrients (calcium,
magnesium, potassium, phosphorus and sulfur) concentration
was studied in tetraploid wheat population of 152
recombinant inbred lines (RILs), derived from a cross
between durum wheat (cv. Langdon) and wild emmer
(accession G18-16). Wide genetic variation was found
among the RILs for all grain minerals, with considerable
transgressive effect. A total of 82 QTLs were mapped for
10 minerals with LOD score range of 3.2–16.7. Most QTLs
were in favor of the wild allele (50 QTLs). Fourteen pairs
of QTLs for the same trait were mapped to seemingly
homoeologous positions, reflecting synteny between the A
and B genomes. Significant positive correlation was found
between grain protein concentration (GPC), Zn, Fe and Cu,
which was supported by significant overlap between the
respective QTLs, suggesting common physiological and/or
genetic factors controlling the concentrations of these
mineral nutrients. Few genomic regions (chromosomes 2A,
5A, 6B and 7A) were found to harbor clusters of QTLs for
GPC and other nutrients. These identified QTLs may
facilitate the use of wild alleles for improving grain
nutritional quality of elite wheat cultivars, especially in
terms of protein, Zn and Fe
The GALEX Arecibo SDSS Survey II: The Star Formation Efficiency of Massive Galaxies
We use measurements of the HI content, stellar mass and star formation rates
in ~190 massive galaxies with stellar masses greater than 10^10 Msun, obtained
from the Galex Arecibo SDSS Survey (GASS) described in Paper I (Catinella et
al. 2010) to explore the global scaling relations associated with the
bin-averaged ratio of the star formation rate over the HI mass, which we call
the HI-based star formation efficiency (SFE). Unlike the mean specific star
formation rate, which decreases with stellar mass and stellar mass surface
density, the star formation efficiency remains relatively constant across the
sample with a value close to SFE = 10^-9.5 yr^-1 (or an equivalent gas
consumption timescale of ~3 Gyr). Specifically, we find little variation in SFE
with stellar mass, stellar mass surface density, NUV-r color and concentration.
We interpret these results as an indication that external processes or feedback
mechanisms that control the gas supply are important for regulating star
formation in massive galaxies. An investigation into the detailed distribution
of SFEs reveals that approximately 5% of the sample shows high efficiencies
with SFE > 10^-9 yr^-1, and we suggest that this is very likely due to a
deficiency of cold gas rather than an excess star formation rate. Conversely,
we also find a similar fraction of galaxies that appear to be gas-rich for
their given specific star-formation rate, although these galaxies show both a
higher than average gas fraction and lower than average specific star formation
rate. Both of these populations are plausible candidates for "transition"
galaxies, showing potential for a change (either decrease or increase) in their
specific star formation rate in the near future. We also find that 36+/-5% of
the total HI mass density and 47+/-5% of the total SFR density is found in
galaxies with stellar mass greater than 10^10 Msun. [abridged]Comment: 18 pages, 11 figures. Accepted for publication in MNRAS. GASS
publications and released data can be found at
http://www.mpa-garching.mpg.de/GASS/index.ph
Inhibitors of trypanosoma cruzi Sir2 related protein 1 as potential drugs against Chagas disease.
Chagas disease remains one of the most neglected diseases in the world despite being the most important parasitic disease in Latin America. The characteristic chronic manifestation of chagasic cardiomyopathy is the region's leading cause of heart-related illness, causing significant mortality and morbidity. Due to the limited available therapeutic options, new drugs are urgently needed to control the disease. Sirtuins, also called Silent information regulator 2 (Sir2) proteins have long been suggested as interesting targets to treat different diseases, including parasitic infections. Recent studies on Trypanosoma cruzi sirtuins have hinted at the possibility to exploit these enzymes as a possible drug targets. In the present work, the T. cruzi Sir2 related protein 1 (TcSir2rp1) is genetically validated as a drug target and biochemically characterized for its NAD+-dependent deacetylase activity and its inhibition by the classic sirtuin inhibitor nicotinamide, as well as by bisnaphthalimidopropyl (BNIP) derivatives, a class of parasite sirtuin inhibitors. BNIPs ability to inhibit TcSir2rp1, and anti-parasitic activity against T. cruzi amastigotes in vitro were investigated. The compound BNIP Spermidine (BNIPSpd) (9), was found to be the most potent inhibitor of TcSir2rp1. Moreover, this compound showed altered trypanocidal activity against TcSir2rp1 overexpressing epimastigotes and anti-parasitic activity similar to the reference drug benznidazole against the medically important amastigotes, while having the highest selectivity index amongst the compounds tested. Unfortunately, BNIPSpd failed to treat a mouse model of Chagas disease, possibly due to its pharmacokinetic profile. Medicinal chemistry modifications of the compound, as well as alternative formulations may improve activity and pharmacokinetics in the future. Additionally, an initial TcSIR2rp1 model in complex with p53 peptide substrate was obtained from low resolution X-ray data (3.5 Å) to gain insight into the potential specificity of the interaction with the BNIP compounds. In conclusion, the search for TcSir2rp1 specific inhibitors may represent a valuable strategy for drug discovery against T. cruzi
The crystal structure of the Leishmania infantum Silent Information Regulator 2 related protein 1: implications to protein function and drug design.
The research leading to these results received funding from the European Community’s Seventh Framework Programme under grant agreement No.602773 (Project KINDRED).The de novo crystal structure of the Leishmania infantum Silent Information Regulator 2 related protein 1 (LiSir2rp1) has been solved at 1.99Å in complex with an acetyl-lysine peptide substrate. The structure is broadly commensurate with Hst2/SIRT2 proteins of yeast and human origin, reproducing many of the structural features common to these sirtuin deacetylases, including the characteristic small zinc-binding domain, and the larger Rossmann-fold domain involved in NAD+-binding interactions. The two domains are linked via a cofactor binding loop ordered in open conformation. The peptide substrate binds to the LiSir2rp1 protein via a cleft formed between the small and large domains, with the acetyl-lysine side chain inserting further into the resultant hydrophobic tunnel. Crystals were obtained only with recombinant LiSir2rp1 possessing an extensive internal deletion of a proteolytically-sensitive region unique to the sirtuins of kinetoplastid origin. Deletion of 51 internal amino acids (P253-E303) from LiSir2rp1 did not appear to alter peptide substrate interactions in deacetylation assays, but was indispensable to obtain crystals. Removal of this potentially flexible region, that otherwise extends from the classical structural elements of the Rossmann-fold, specifically the β8-β9 connector, appears to result in lower accumulation of the protein when expressed from episomal vectors in L. infantum SIR2rp1 single knockout promastigotes. The biological function of the large serine-rich insertion in kinetoplastid/trypanosomatid sirtuins, highlighted as a disordered region with strong potential for post-translational modification, remains unknown but may confer additional cellular functions that are distinct from their human counterparts. These unique molecular features, along with the resolution of the first kinetoplastid sirtuin deacetylase structure, present novel opportunities for drug design against a protein target previously established as essential to parasite survival and proliferation.Publisher PDFPeer reviewe
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