Holocene record of Tuggerah Lake estuary development on the Australian east coast: sedimentary responses to sea-level fluctuations and climate variability

We investigated the Holocene palaeo-environmental record of the Tuggerah Lake barrier estuary on the south-east coast of Australia to determine the influence of local, regional and global environmental changes on estuary development. Using multi-proxy approaches, we identified significant down-core variation in sediment cores relating to sea-level rise and regional climate change. Following erosion of the antecedent land surface during the post-glacial marine transgression, sediment began to accumulate at the more seaward location at ~8500. years before present, some 1500. years prior to barrier emplacement and ~4000. years earlier than at the landward site. The delay in sediment accumulation at the landward site was a consequence of exposure to wave action prior to barrier emplacement, and due to high river flows of the mid-Holocene post-barrier emplacement. As a consequence of the mid-Holocene reduction in river flows, coupled with a moderate decline in sea-level, the lake experienced major changes in conditions at ~4000. years before present. The entrance channel connecting the lake with the ocean became periodically constricted, producing cyclic alternation between intervals of fluvial- and marine-dominated conditions. Overall, this study provides a detailed, multi-proxy investigation of the physical evolution of Tuggerah Lake with causative environmental processes that have influenced development of the estuary

Do ENSO and coastal development enhance coastal burial of terrestrial carbon?

Carbon cycling on the east coast of Australia has the potential to be strongly affected by El Niño-Southern Oscillation (ENSO) intensification and coastal development (industrialization and urbanization). We performed paleoreconstructions of estuarine sediments from a seagrass-dominated estuary on the east coast of Australia (Tuggerah Lake, New South Wales) to test the hypothesis that millennial-scale ENSO intensification and European settlement in Australia have increased the transfer of organic carbon from land into coastal waters. Our data show that carbon accumulation rates within coastal sediments increased significantly during periods of maximum millennial-scale ENSO intensity ("super-ENSO") and coastal development. We suggest that ENSO and coastal development destabilize and liberate terrestrial soil carbon, which, during rainfall events (e.g., La Niña), washes into estuaries and becomes trapped and buried by coastal vegetation (seagrass in this case). Indeed, periods of high carbon burial were generally characterized as having rapid sedimentation rates, higher content of fine-grained sediments, and increased content of wood and charcoal fragments. These results, though preliminary, suggest that coastal development and ENSO intensificationboth of which are predicted to increase over the coming centurycan enhance capture and burial of terrestrial carbon by coastal ecosystems. These findings have important relevance for current efforts to build an understanding of terrestrial- marine carbon connectivity into global carbon budgets

CTCF variants in 39 individuals with a variable neurodevelopmental disorder broaden the mutational and clinical spectrum

Purpose: Pathogenic variants in the chromatin organizer CTCF were previously reported in seven individuals with a neurodevelopmental disorder (NDD). Methods: Through international collaboration we collected data from 39 subjects with variants in CTCF. We performed transcriptome analysis on RNA from blood samples and utilized Drosophila melanogaster to investigate the impact of Ctcf dosage alteration on nervous system development and function. Results: The individuals in our cohort carried 2 deletions, 8 likely gene-disruptive, 2 splice-site, and 20 different missense variants, most of them de novo. Two cases were familial. The associated phenotype was of variable severity extending from mild developmental delay or normal IQ to severe intellectual disability. Feeding difficulties and behavioral abnormalities were common, and variable other findings including growth restriction and cardiac defects were observed. RNA-sequencing in five individuals identified 3828 deregulated genes enriched for known NDD genes and biological processes such as transcriptional regulation. Ctcf dosage alteration in Drosophila resulted in impaired gross neurological functioning and learning and memory deficits. Conclusion: We significantly broaden the mutational and clinical spectrum of CTCF-associated NDDs. Our data shed light onto the functional role of CTCF by identifying deregulated genes and show that Ctcf alterations result in nervous system defects in Drosophila.Peer reviewe

The development and validation of a scoring tool to predict the operative duration of elective laparoscopic cholecystectomy

Background: The ability to accurately predict operative duration has the potential to optimise theatre efficiency and utilisation, thus reducing costs and increasing staff and patient satisfaction. With laparoscopic cholecystectomy being one of the most commonly performed procedures worldwide, a tool to predict operative duration could be extremely beneficial to healthcare organisations. Methods: Data collected from the CholeS study on patients undergoing cholecystectomy in UK and Irish hospitals between 04/2014 and 05/2014 were used to study operative duration. A multivariable binary logistic regression model was produced in order to identify significant independent predictors of long (> 90 min) operations. The resulting model was converted to a risk score, which was subsequently validated on second cohort of patients using ROC curves. Results: After exclusions, data were available for 7227 patients in the derivation (CholeS) cohort. The median operative duration was 60 min (interquartile range 45–85), with 17.7% of operations lasting longer than 90 min. Ten factors were found to be significant independent predictors of operative durations > 90 min, including ASA, age, previous surgical admissions, BMI, gallbladder wall thickness and CBD diameter. A risk score was then produced from these factors, and applied to a cohort of 2405 patients from a tertiary centre for external validation. This returned an area under the ROC curve of 0.708 (SE = 0.013, p  90 min increasing more than eightfold from 5.1 to 41.8% in the extremes of the score. Conclusion: The scoring tool produced in this study was found to be significantly predictive of long operative durations on validation in an external cohort. As such, the tool may have the potential to enable organisations to better organise theatre lists and deliver greater efficiencies in care

Holocene millennial/centennial-scale multiproxy cyclicity in temperate eastern Australian estuary sediments

We have undertaken a comparative study of down-core variation in multiproxy palaeoclimate data (magnetic susceptibility, calcium carbonate content and total organic carbon) from two coastal water bodies (Myall and Tuggerah Lakes) in temperate eastern Australia to identify local, regional and global-forcing factors within Holocene estuarine sediments. The two lakes lie within the same temperate climate zone adjacent to the Tasman Sea, but are not part of the same catchment and drain different geological provinces. One is essentially a freshwater coastal lake whereas the other is a brackish back-barrier lagoon. Despite these differences, data from two sites in each of the two lakes have allowed us to investigate and compare cyclicity in otherwise uniform, single facies sediments within the frequency range of 200-2000 years, limited by the sedimentation rate within the lakes and our sample requirements. We have auto- and cross-correlated strong periodicities at similar to 360 years, similar to 500-530 years, similar to 270-290 years, 420-450 years and similar to 210 years, and subordinate periods of similar to 650 years, 1200-1400 years and similar to 1800 years. Our thesis is that climate is the only regionally available mechanism available to control common millennial and centennial scale cyclicity in these sediments, given the geographical and other differences. However, regional climate may not be the dominant effect at any single time and either location. Within the range of frequency spectral peaks we have identified, several fall within known long-term periodical fluctuations of sun spot activity; however, feedback loops associated with short-term orbital variation, such as Dansgaard-Oeschger cycles, and the relationship between these and palaeo-ENSO variation, are also possible contributors

Relationship between sediment % total organic carbon (%TOC) and mud content.

<p>Data taken from the <63 μm fraction from unit 1 and 2 sediments (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145136#pone.0145136.g002" target="_blank">Fig 2</a>).</p

Sediment cores taken from Chittaway Bay and Pelican Island showing how sediment type changes with depth (cm) down core and age (¹⁴C data, mean ± SE).

<p>Sediment cores taken from Chittaway Bay and Pelican Island showing how sediment type changes with depth (cm) down core and age (¹⁴C data, mean ± SE).</p

Age-models of sediment accumulation rates.

<p>Age-models of sediment accumulation rates.</p

Sediment archives showing geochemical changes during the past 9000 years.

<p>Increased foraminifera abundance, % organic carbon (C), organic C accumulation rate (C_org A.R.), and % mud are observed during ENSO intensification (grey-shaded area) and since European settlement (~200 years ago). Darker shading indicates the peak period following re-establishment of ENSO after the mid-Holocene hiatus (Gagan et al. 2004). Sediment cores taken from Tuggerah Lake, NSW, Australia (Sites: PI—Pelican Island, CB—Chittaway Bay).</p