Proceso de contratación y selección de personal, orientado a la satisfacción de las necesidades, criterios y objetivos, de las áreas específicas de la empresa Laboratorios Siegfried S.A.S

Anexo 1 (Procedimiento de incorporación interna) - Anexo 2 (Check-list de Verificación)Este trabajo tiene como fin comprender y analizar políticas y estrategias utilizadas en el proceso de selección de personal para cubrir vacantes en el área de Trademarketing de la empresa “Laboratorios Siegfried S.A.S”., y con ello poder identificar las falencias presentadas en el proceso, hallar las mejores alternativas para optimizarlo y brindar las recomendaciones pertinentes que contribuyan al mejoramiento del área de Talento Humano. A través de un método de investigación Cualitativa, basada en la obtención de información y observación directa y con entrevistas realizadas a la Gerente de Talento Humano se encontró que el proceso de selección de candidatos se realiza a través de agentes externos y de forma interna en el área de Talento Humano, donde se eligen a los candidatos adecuados que cumplan los requisitos para que ocupen los diferentes cargos y se logren los objetivos propuestos por la empresa. Del análisis de la información se pudo evidenciar, que la mayor falencia e incidencia en la rotación de personal en el área de Trademarketing, se da con el personal vinculado a través de agencias externas (90%), por lo tanto se recomienda fortalecer el área de Talento Humano de la empresa y que sea esta la encargada de realizar todos los procesos de vinculación, con unas políticas, procesos y procedimientos claros, eficaces y eficientes que permitan encontrar los mejores candidatos y con los mejores perfiles que requiere la empresa Laboratorios Siegfried S.A.S.The purpose of this work is to understand and analyze policies and strategies used in the personnel selection process to fill vacancies in the Trademarketing area of the company “Laboratorios Siegfried SAS”, and thus be able to identify the shortcomings presented in the process, find the best alternatives to optimize it and provide the pertinent recommendations that contribute to the improvement of the Human Talent area. Through a Qualitative research method, based on obtaining information and direct observation and with interviews with the Human Talent Manager, it was found that the personnel selection process is carried out through external agents and internally in the Human Talent area, where suitable candidates are chosen who meet the requirements to occupy the different positions and achieve the objectives proposed by the company. From the analysis of the information, it was possible to show that the greatest deficiency and incidence in the turnover of personnel in the Trademarketing area, occurs with personnel linked through external agencies (90%), therefore it is recommended to strengthen the area of Human Talent of the company and that it is in charge of carrying out all the linking processes, with clear, effective and efficient policies, processes and procedures that allow finding the best candidates and with the best profiles required by the company Siegfried SAS Laboratorie

Altered protein expression and protein nitration pattern during d-galactosamine-induced cell death in human hepatocytes: a proteomic analysis

BACKGROUND/AIMS: Hepatic injury by d-galactosamine (d-GalN) is a suitable experimental model of hepatocellular injury. The induction of oxidative and nitrosative stress participates during d-GalN-induced cell death in cultured rat hepatocytes. This study aimed to identify protein expression changes during the induction of apoptosis and necrosis by d-GalN in cultured human hepatocytes. METHODS: A proteomic approach was used to identify the proteins involved and those altered by tyrosine nitration. A high dose of d-GalN (40 mM) was used to induce apoptosis and necrosis in primary culture of human hepatocytes. Cellular lysates prepared at different times after addition of d-GalN were separated by two-dimensional electrophoresis. Gel spots with an altered expression and those matching nitrotyrosine-immunopositive proteins were excised and analyzed by mass spectrometry. RESULTS: d-GalN treatment upregulated microsomal cytochrome b5, fatty acid binding protein and manganese superoxide dismutase, and enhanced annexin degradation. d-GalN increased tyrosine nitration of four cytosolic (Hsc70, Hsp70, annexin A4 and carbonyl reductase) and three mitochondrial (glycine amidinotransferase, ATP synthase beta chain, and thiosulfate sulfurtransferase) proteins in human hepatocytes. CONCLUSIONS: The results provide evidences that oxidative stress and nitric oxide-derived reactive oxygen intermediates induce specific alterations in protein expression that may be critical for the induction of apoptosis and necrosis by d-GalN in cultured human hepatocytes

Cardiovascular Risk in Systemic Autoimmune Diseases: Epigenetic Mechanisms of Immune Regulatory Functions

Autoimmune diseases (AIDs) have been associated with accelerated atherosclerosis (AT) leading to increased cardio- and cerebrovascular disease risk. Traditional risk factors, as well as systemic inflammation mediators, including cytokines, chemokines, proteases, autoantibodies, adhesion receptors, and others, have been implicated in the development of these vascular pathologies. Yet, the characteristics of vasculopathies may significantly differ depending on the underlying disease. In recent years, many new genes and signalling pathways involved in autoimmunity with often overlapping patterns between different disease entities have been further detected. Epigenetics, the control of gene packaging and expression independent of alterations in the DNA sequence, is providing new directions linking genetics and environmental factors. Epigenetic regulatory mechanisms comprise DNA methylation, histone modifications, and microRNA activity, all of which act upon gene and protein expression levels. Recent findings have contributed to our understanding of how epigenetic modifications could influence AID development, not only showing differences between AID patients and healthy controls, but also showing how one disease differs from another and even how the expression of key proteins involved in the development of each disease is regulated

Method based on data mining techniques for breast cancer recurrence analysis

Cancer is a constantly evolving disease, which affects a large number of people worldwide. Great efforts have been made at the research level for the development of tools based on data mining techniques that allow to detect or prevent breast cancer. The large volumes of data play a fundamental role according to the literature consulted, a great variety of dataset oriented to the analysis of the disease has been generated, in this research the Breast Cancer dataset was used, the purpose of the proposed research is to submit comparison of the J48 and randomforest, NaiveBayes and NaiveBayes Simple, SMO Poli-kernel and SMO RBF-Kernel classification algorithms, integrated with the Simple K-Means cluster algorithm for the generation of a model that allows the successful classification of patients who are or Non-recurring breast cancer after having previously undergone surgery for the treatment of said disease, finally the methods that obtained the best levels were SMO Poly-Kernel + Simple K-Means 98.5% of Precision, 98.5% recall, 98.5% TPRATE and 0.2% FPRATE. The results obtained suggest the possibility of using intelligent computational tools based on data mining methods for the detection of breast cancer recurrence in patients who had previously undergone surgery

A genetic approach reveals different modes of action of prefoldins

[EN] The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved in eukaryotes and is composed of subunits PFD1-6, and PFDc-TRiC/CCT folds actin and tubulins. PFDs also participate in a wide range of cellular processes, both in the cytoplasm and in the nucleus, and their malfunction causes developmental alterations and disease in animals and altered growth and environmental responses in yeast and plants. Genetic analyses in yeast indicate that not all of their functions require the canonical complex. The lack of systematic genetic analyses in plants and animals, however, makes it difficult to discern whether PFDs participate in a process as the canonical complex or in alternative configurations, which is necessary to understand their mode of action. To tackle this question, and on the premise that the canonical complex cannot be formed if one subunit is missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient in the six PFDs and compared various growth and environmental responses with those of the individual mutants. In this way, we demonstrate that the PFDc is required for seed germination, to delay flowering, or to respond to high salt stress or low temperature, whereas at least two PFDs redundantly attenuate the response to osmotic stress. A coexpression analysis of differentially expressed genes in the sextuple mutant identified several transcription factors, including ABA INSENSITIVE 5 (ABI5) and PHYTOCHROME-INTERACTING FACTOR 4, acting downstream of PFDs. Furthermore, the transcriptomic analysis allowed assigning additional roles for PFDs, for instance, in response to higher temperature.This work was supported by grants from the Spanish Ministry of Economy and Competitiveness and "Agencia Estatal de Investigacion"/FEDER/European Union (BIO2013-43184-P to D.A. and M.A.B., and BIO2016-79133-P and PID2019-109925GB-I00 to D.A.). N.B.-T., A.S.-M., and A.P.-A. were recipient of Ministerio de Economia y Competitividad (BES-2014-068868), EU MSCA-IF (H2020-MSCA-IF-2016746396) and Ministerio de Educacion (FPU17/05186) fellowships, respectively.Esteve-Bruna, D.; Blanco-Touriñán, N.; Serrano-Mislata, A.; Esquinas-Ariza, RM.; Resentini, F.; Forment Millet, JJ.; Carrasco-López, C.... (2021). A genetic approach reveals different modes of action of prefoldins. Plant Physiology. 187(3):1534-1550. https://doi.org/10.1093/plphys/kiab348S15341550187

Osteosynthesis-associated infection of the lower limbs by multidrug-resistant and extensively drug-resistant Gram-negative bacteria: a multicentre cohort study

Purpose: The purpose of this study was the clinical and therapeutic assessment of lower-limb osteosynthesis-associated infection (OAI) by multidrug-resistant (MDR) and extensively drug-resistant (XDR) Gram-negative bacteria (GNB), which have been poorly studied to date. Methods: A prospective multicentre observational study was conducted on behalf of ESGIAI (the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Implant-Associated Infections). Factors associated with remission of the infection were evaluated by multivariate and Cox regression analysis for a 24-month follow-up period. Results: Patients (n=57) had a history of trauma (87.7 %), tumour resection (7 %) and other bone lesions (5.3 %). Pathogens included Escherichia coli (n=16), Pseudomonas aeruginosa (n=14; XDR 50 %), Klebsiella spp. (n=7), Enterobacter spp. (n=9), Acinetobacter spp. (n=5), Proteus mirabilis (n=3), Serratia marcescens (n=2) and Stenotrophomonas maltophilia (n=1). The prevalence of ESBL (extended-spectrum β-lactamase), fluoroquinolone and carbapenem resistance were 71.9 %, 59.6 % and 17.5 % respectively. Most patients (n=37; 64.9 %) were treated with a combination including carbapenems (n=32) and colistin (n=11) for a mean of 63.3 d. Implant retention with debridement occurred in early OAI (66.7 %), whereas the infected device was removed in late OAI (70.4 %) (p=0.008). OAI remission was achieved in 29 cases (50.9 %). The type of surgery, antimicrobial resistance and duration of treatment did not significantly influence the outcome. Independent predictors of the failure to eradicate OAI were age &gt;60 years (hazard ratio, HR, of 3.875; 95 % confidence interval, CI95 %, of 1.540–9.752; p=0.004) and multiple surgeries for OAI (HR of 2.822; CI95 % of 1.144–6.963; p=0.024). Conclusions: Only half of the MDR/XDR GNB OAI cases treated by antimicrobials and surgery had a successful outcome. Advanced age and multiple surgeries hampered the eradication of OAI. Optimal therapeutic options remain a challenge.</p