Reading In-Between the Lines: Exploring the Experience of Cultivating Cultural Awareness with White Teacher Candidates in a Liberal Arts University in Georgia

This study was to explore the use of critical literacy (Freire, 1998) to assist predominately White teacher candidates in their preparation to teach diverse groups of students. Seven White females, who were born and reared in Georgia, participated in the study. These participants were encouraged to critically examine their personal, racial, and cultural roots through autobiographical papers, literature circles using African American children\u27s books, critical reflective papers responding to the literature circles, conversations, and interviews. Three bodies of research provided the theoretical framework for the study: White identity theory (Kunjufu, 2002, McIntyre, 1997, Wise, 2008), critical literacy theory (Freire, 1974, Freire & Macedo, 1987, Macedo & Steinberg, 2007, Vasquez, 2003, 2004), and culturally relevant pedagogy (Ayers, 2004, Delpit, 1995, Gay, 2000, hooks, 1994, Howard, 2006 ,Ladson-Billings, 1994). The study was conducted using two distinct strands of inquiry, narrative inquiry (Clandinin & Connelly, 2000) and personal̋~passionate̋~participatory inquiry (He & Phillion, 2008). Engaging in research that is personal̋~ passionate̋~ participatory creates possibilities for positive change in the researcher, participants, and ultimately in society. This form of inquiry allows researchers to connect the practical with the theoretical, and the personal with the political, through passionate participation in, and critical reflection upon inquiry and life (p. 3). Part of the challenge for this study was to confront the resistance to challenging Whiteness, to use literature circles to create a space for the hearts and minds to develop critical consciousness and cultural awareness, to transgress White supremacy, and to embrace multitudes of differences, contradictions, and complexities in schools, neighborhoods, and communities. This study has awakened me intellectually as I continue to live my life as a member of a racist society and a teacher educator in a predominately White College of Education. As the public school populations become increasingly diversified, the number of White teacher candidates continues to increase. As education within the South and beyond still perpetuates White privilege, relinquishing White supremacy and status quo is the prelude to cultivating cultural awareness in White teacher candidates. Teacher educators need to work with learners, teachers, parents, community workers, administrators, and policy makers to support and encourage a culturally relevant pedagogy and to create culturally responsive and inspiring learning environments to engage all learners in an increasingly diversified world

Providing Early Childhood Pre-Service Teachers with Increased Science Content Knowledge and Effective Teaching Strategies: A Two Year Project

This presentation explores the results of a two-year project with pre-service teachers which focused primarily on providing increased knowledge of science concepts, effective strategies, and use of resources to improve science instruction while increasing confidence for the teaching of science

Barriers and facilitators to asthma self-management in adolescents:a systematic review of qualitative and quantitative studies

BACKGROUND: Many adolescents have poor asthma control and impaired quality of life despite the availability of modern pharmacotherapy. Research suggests that poor adherence to treatment and limited engagement in self-management could be contributing factors. OBJECTIVE: To conduct a systematic review of the barriers and facilitators to self-management of asthma reported by adolescents using a narrative synthesis approach to integrate the findings. DESIGN: MEDLINE, EMBASE, CINAHL, and PsycINFO were searched for all types of study design. Full papers were retrieved for study abstracts that included data from participants aged 12-18 years referring to barriers or facilitators of asthma self-management behaviors. RESULTS: Sixteen studies (5 quantitative and 11 qualitative) underwent data extraction, quality appraisal, and thematic analysis. Six key themes were generated that encompassed barriers and/or facilitators to self-management of asthma in adolescents: Knowledge, Lifestyle, Beliefs and Attitudes, Relationships, Intrapersonal Characteristics, and Communication. CONCLUSIONS: There is a pressing need to prepare adolescents for self-management, using age-appropriate strategies that draw on the evidence we have synthesized. Current clinical practice should focus on ensuring adolescents have the correct knowledge, beliefs, and positive attitude to self-manage their illness. This needs to be delivered in a supportive environment that facilitates two-way communication, fosters adolescents' self-efficacy to manage their disease, and considers the wider social influences that impinge on self-management. Pediatr Pulmonol. 2016; 9999:XX-XX. © 2016 Wiley Periodicals, Inc

Isolation of an autocrine growth factor from hepatoma HTC‐SR cells

A growth factor has been isolated from HTC‐SR rat hepatoma tissue culture cells which specifically stimulates DNA synthesis and cell proliferation of the HTC cells that produce it. The factor can be isolated from HTC cell conditioned medium or from an HTC cell extract. This autocrine factor has been purified 640‐fold from a postmicrosomal supernatant by successive steps, involving ethanol precipitation, heating at 80°C for 10 min, chromatography on a DEAE Bio‐Gel A column, and chromatography on a heparin‐sepharose affinity column. The major peak of activity eluted from the heparin column migrates as a single band on SDS‐PAGE with an apparent Mr of 60,000. The factor is resistant to acid, heat, and neuraminidase but sensitive to trypsin, papain, and protease. The autocrine nature of the factor is indicated by the finding that several other types of cells do not respond with increased DNA synthesis. Mouse L‐cells, BHK cells, Novikoff hepatoma cells, hepatocytes in primary culture, and an epithelial‐like rat liver‐derived cell line (Clone 9) were tested, and none of the cells could be stimulated. Small amounts of the factor could be extracted from the Clone 9 cells, however. This material had the same physical and purification properties as the factor extracted from HTC cells, but it did not stimulate DNA synthesis in Clone 9 cells, only in HTC cells. Addition of the factor resulted in an almost immediate stimulation of DNA synthesis in a proliferating HTC cell population. When the factor was added together with [3H]thymidine for 2 h, a significant stimulation of DNA synthesis was observed, provided the addition was made between 18 and 48 h after the cells had been plated. Autoradiographic studies indicated that the factor both accelerates DNA synthesis in cells already making DNA and increases the number of cells entering the S period. The stimulation of DNA synthesis was completely inhibited by 10 mM hydroxyurea, whether the factor was present for 2, 24, or 48 h in the culture. A significant increase in cell number due to addition of the factor was also observed. This accelerated proliferation was detectable only after the cells had been in culture for at least 48 h with the factor present. Copyright © 1987 Wiley‐Liss, Inc

The genetics of virus particle shape in equine influenza A virus

Background Many human strains of influenza A virus produce highly pleomorphic virus particles that at the extremes can be approximated as either spheres of around 100 nm diameter or filaments of similar cross-section but elongated to lengths of many microns. The role filamentous virions play in the virus life cycle remains enigmatic. Objectives/Methods Here, we set out to define the morphology and genetics of virus particle shape in equine influenza A virus, using reverse genetics and microscopy of infected cells. Results and Conclusions The majority of H3N8 strains tested were found to produce filamentous virions, as did the prototype H7N7 A/eq/Prague/56 strain. The exception was the prototype H3N8 isolate, A/eq/Miami/63. Reassortment of equine influenza virus M genes from filamentous and non-filamentous strains into the non-filamentous human virus A/PR/8/34 confirmed that segment 7 is a major determinant of particle shape. Sequence analysis identified three M1 amino acid polymorphisms plausibly associated with determining virion morphology, and the introduction of these changes into viruses confirmed the importance of two: S85N and N231D. However, while either change alone affected filament production, the greatest effect was seen when the polymorphisms were introduced in conjunction. Thus, influenza A viruses from equine hosts also produce filamentous virions, and the major genetic determinants are set by the M1 protein. However, the precise sequence determinants are different to those previously identified in human or porcine viruses

Isolation, characterization, and substrate properties of the external limiting membrane from the avian embryonic optic tectum

The external limiting membrane of the avian embryonic optic tectum is isolated by mechanically separating the neuronal mesencephalon from the overlying mesenchymal tissue. The preparation consists of a basal lamina which is covered on its neural side by endfeet of neuroepithelial cells and has attached to it on its meningeal side a collageneous stroma, containing blood vessels. The external limiting membrane can be flat-mounted on a piece of nitrocellulose filter as mechanical support. It covers an area between 0.3 and 1 the cm2, depending on the age of me donor embryo. The endfeet can be removed together with all cellular components of the meninges by treatment with 2% Triton-X-100 or with distilled water. The basal lamina itself is approximately 80 nm thick and consists of two laminae rarae and a central lamina densa. Immunohistochemical staining reveals that the basal lamina in the embryo, after isolation and after detergent extraction of the isolated preparation, contains type IV collagen, nidogen, laminin, and low density heparan sulfate proteoglycan as do other basement membranes. Antibodies against the neural cell adhesion molecule (N-CAM), chondroitin sulfate proteoglycan, and fibronectin fail to stain the external limiting membrane, but these proteins were clearly identified in the blood vessel-containing meninges or in the optic tectum. The flat-mounted external limiting membrane preparation was used as substrate to culture several different neural tissues of central and peripheral origin. Explants of neural crest cells, dorsal root ganglia, and sympathetic ganglia can be cultured on the external limiting membrane. All explants grow well on the basal lamina preparations whether the endfeet are attached or detergent-extracted prior to explantation; however, neurite outgrowth from sympathetic ganglia is reduced in the presence of the endfeet. Although the endfoot-lined external limiting membrane represents at least part of the immediate environment encountered by retinal axons as they invade the optic tectum and despite its excellent properties as a substrate for retinal axons in vitro, cues guiding the orientation of axons were not detected in the flat-mounted preparation

Supermassive Black Hole Growth and Merger Rates from Cosmological N-body Simulations

Understanding how seed black holes grow into intermediate and supermassive black holes (IMBHs and SMBHs, respectively) has important implications for the duty-cycle of active galactic nuclei (AGN), galaxy evolution, and gravitational wave astronomy. Most studies of the cosmological growth and merger history of black holes have used semianalytic models and have concentrated on SMBH growth in luminous galaxies. Using high resolution cosmological N-body simulations, we track the assembly of black holes over a large range of final masses -- from seed black holes to SMBHs -- over widely varying dynamical histories. We used the dynamics of dark matter halos to track the evolution of seed black holes in three different gas accretion scenarios. We have found that growth of Sagittarius A* - size SMBH reaches its maximum mass M_{SMBH}~10^6Msun at z~6 through early gaseous accretion episodes, after which it stays at near constant mass. At the same redshift, the duty-cycle of the host AGN ends, hence redshift z=6 marks the transition from an AGN to a starburst galaxy which eventually becomes the Milky Way. By tracking black hole growth as a function of time and mass, we estimate that the IMBH merger rate reaches a maximum of R_{max}=55 yr^-1 at z=11. From IMBH merger rates we calculate N_{ULX}=7 per Milky Way type galaxy per redshift in redshift range 2<z<6.Comment: 12 pages, 8 figures, submitted to MNRA

Functional Genomics Pipeline in Loblolly Pine and Eastern Cottonwood

Advances in transformation technology allow functional genomics techniques that are commonly applied to Arabidopsis thaliana to be considered for tree species. ArborGen is applying the principles of high throughput functional genomics originally developed for Arabidopsis to both hardwood and conifer trees as a means to screening genes that affect wood quality traits and productivity. The ability to demonstrate gene function in commercially important tree species is an essential technology for developing improved tree products based on gene transfer. Starting with a large database of ESTs isolated from Pinus radiata and Eucalyptus grandis, a systematic approach to uncovering gene function is in progress. This integrated functional screen consists of bioinformatics characterization, cell-based assays, and Arabidopsis thaliana screens to identify candidates for high throughput functional testing in two commercially important species: Populus deltoides (Eastern cottonwood) and Pinus taeda (Loblolly pine). Efficient gene transfer methods are being used to introduce large numbers of genes into these tree species and methods for early detection of transgene function are being developed based on phenotypic and chemical composition screens. These methods will enable ArborGen to functionally test several hundred genes per year in commercial tree species. These functional screens in trees are being used to identify candidate genes that are expected to affect key commercial traits in plantation forestry. This integrated system for characterizing tree genes including bioinformatics, cell-based assays, Arabidopsis screens, and high throughput Pine and Populus screening systems will be described.Papers and abstracts from the 27th Southern Forest Tree Improvement Conference held at Oklahoma State University in Stillwater, Oklahoma on June 24-27, 2003

Barriers and facilitators to self-management of asthma in adolescents:an interview study to inform development of a novel intervention

BACKGROUND AND OBJECTIVE: Despite literature that spans twenty years describing the barriers to asthma self-management in adolescents, successful, clinically-based interventions to address this important issue are lacking. Given the limitations of some of the previous studies, we conducted a study that aimed to gain a broader insight into barriers and facilitators to self-management of asthma by adolescents, not just adherence to treatment, and triangulated their views with those of their parents and healthcare professionals. METHODS: Focus groups and interviews were conducted separately for 28 adolescents with asthma aged 12-18 years, 14 healthcare professionals, and 12 parents. Focus groups and interviews were audio-recorded and transcripts from each participant group were analysed separately using inductive thematic analysis. We triangulated the three perspectives by comparing themes that had emerged from each analysis. RESULTS: Adolescents', parents', and healthcare professionals' views were summarised into ten related themes that included forgetting and routines, knowledge, embarrassment and confidence, communication with healthcare professionals, triggers, support at school, apathy, and taking responsibility. We found that adolescents, parents and healthcare professionals raised similar barriers and facilitators to self-management and our results provide further validation for previous studies. CONCLUSION AND CLINICAL RELEVANCE: Our study highlights that healthcare professionals may need to consider a range of psychological and contextual issues influencing adolescents' ability to effectively self-manage their asthma, in particular, how they implement treatment routines and the understanding that adolescents have of their condition and treatments. Crucially, healthcare professionals need to consider how this information is communicated and ensure they facilitate open, inclusive, two-way consultations. From this more comprehensive understanding, we have developed interventional strategies that healthcare professionals can utilise to empower adolescents to improve their asthma self-management. This article is protected by copyright. All rights reserved