Les problématiques de diagnostic et de projection dans les PMI

National audienceQuelle est l'attitude actuelle des PMI françaises à l'égard des pratiques de planification? Cette question est abordée en revenant sur la littérature se rapportant à la planification, afin d'élaborer un cadre d'analyse pertinent susceptible de faciliter l'observation des pratiques dans les PMI. À partir d'une méthodologie quantitative, les résultats issus de l'analyse de 82 situations permettent de mieux comprendre la réalité de la planification et, notamment, de lister les variables de contingence mises en avant. L'article débouche sur un essai de classification typologique des PMI en fonction de leurs attitudes et de leurs pratiques face à la planification

BCB Based Packaging for Low Actuation Voltage RF MEMS Devices

This paper outlines the issues related to RF MEMS packaging and low actuation voltage. An original approach is presented concerning the modeling of capacitive contacts using multiphysics simulation and advanced characterization. A similar approach is used concerning packaging development where multi-physics simulations are used to optimize the process. A devoted package architecture is proposed featuring very low loss at microwave range

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation.

RNA Polymerase II (Pol II) and transcription factors form concentrated hubs in cells via multivalent protein-protein interactions, often mediated by proteins with intrinsically disordered regions. During Herpes Simplex Virus infection, viral replication compartments (RCs) efficiently enrich host Pol II into membraneless domains, reminiscent of liquid-liquid phase separation. Despite sharing several properties with phase-separated condensates, we show that RCs operate via a distinct mechanism wherein unrestricted nonspecific protein-DNA interactions efficiently outcompete host chromatin, profoundly influencing the way DNA-binding proteins explore RCs. We find that the viral genome remains largely nucleosome-free, and this increase in accessibility allows Pol II and other DNA-binding proteins to repeatedly visit nearby DNA binding sites. This anisotropic behavior creates local accumulations of protein factors despite their unrestricted diffusion across RC boundaries. Our results reveal underappreciated consequences of nonspecific DNA binding in shaping gene activity, and suggest additional roles for chromatin in modulating nuclear function and organization

Avant-propos

Au XXe siècle, l’introduction des processus industriels dans le domaine de l’alimentation a transformé radicalement les filières de production alimentaires : production proprement dite, transformation et distribution en ont été métamorphosées. De même, la production massive de variétés horticoles destinées à la parfumerie, à la cosmétique, à la pharmaceutique ou au marché floral s’est considérablement homogénéisée, favorisant la sélection de variétés performantes et productives, produites par..

Efficacy of Chronic Antidepressant Treatments in a New Model of Extreme Anxiety in Rats

Animal models of anxious disorders found in humans, such as panic disorder and posttraumatic stress disorder, usually include spontaneous and conditioned fear that triggers escape and avoidance behaviors. The development of a panic disorder model with a learned component should increase knowledge of mechanisms involved in anxiety disorders. In our ethological model of extreme anxiety in the rat, forced apnea was combined with cold water vaporization in an inescapable situation. Based on the reactions of vehicle controls, behaviors involved in paroxysmic fear were passive (freezing) and active (jumping) reactions. Our results show that subchronic fluoxetine (5 mg/kg, IP, 21 days) and imipramine (10 mg/kg, IP, 14 days) administration alleviated freezing and jumping behaviors, whereas acute fluoxetine (1 mg/kg, IP) provoked opposite effects. Acute low dose of diazepam (1 mg/kg, IP) was not effective, whereas the higher dose of 3 mg/kg, IP, and clonazepam (1 mg/kg, IP) only had an effect on jumping. Paroxysmic fear generated in this experimental condition may therefore mimic the symptomatology observed in patients with anxiety disorders

Ultramafic rocks in the North Patagonian Andes: is their emplacement associated with the Neogene tectonics of the Liquiñe–Ofqui Fault Zone?

Serpentinites and fresh or partially serpentinized harzburgite crop out in the western slope of the North Patagonian Andes of continental Chiloé (41°44’-42°12’S). These rocks are spatially associated with low-grade metamorphic rocks containing Cenozoic detrital zircons. The metamorphic rocks, together with Devonian metasediments, have been mapped previously as Late Paleozoic-Triassic metamorfic complex, an age no longer tenable for at least part of the complex. Transpressional tectonic emplacement of the ultramafic body or bodies is thought to have been related to activity on the Liquiñe-Ofqui Fault Zone, following a late Oligocene-Early Miocene extensional phase in the forearc region of the present Andes. This fault zone occurs immediately east of the outcrops of the ultramafic rocks and has been interpreted previously as generating a hemi-flower or flower structure

Pepper Mild Mottle Virus, a Plant Virus Associated with Specific Immune Responses, Fever, Abdominal Pains, and Pruritus in Humans

Background: Recently, metagenomic studies have identified viable Pepper mild mottle virus (PMMoV), a plant virus, in the stool of healthy subjects. However, its source and role as pathogen have not been determined. Methods and Findings: 21 commercialized food products containing peppers, 357 stool samples from 304 adults and 208 stool samples from 137 children were tested for PMMoV using real-time PCR, sequencing, and electron microscopy. Anti-PMMoV IgM antibody testing was concurrently performed. A case-control study tested the association of biological and clinical symptoms with the presence of PMMoV in the stool. Twelve (57%) food products were positive for PMMoV RNA sequencing. Stool samples from twenty-two (7.2%) adults and one child (0.7%) were positive for PMMoV by real-time PCR. Positive cases were significantly more likely to have been sampled in Dermatology Units (p<10−6), to be seropositive for anti-PMMoV IgM antibodies (p = 0.026) and to be patients who exhibited fever, abdominal pains, and pruritus (p = 0.045, 0.038 and 0.046, respectively). Conclusions: Our study identified a local source of PMMoV and linked the presence of PMMoV RNA in stool with a specific immune response and clinical symptoms. Although clinical symptoms may be imputable to another cofactor, including spicy food, our data suggest the possibility of a direct or indirect pathogenic role of plant viruses in humans