1,459 research outputs found
High-latitude artificial aurora using the EISCAT high-gain HF facility
The EISCAT high-frequency (HF) transmitter facility at Ramfjord, Norway, has been used to accelerate F-region electrons sufficiently to excite the oxygen atoms and nitrogen molecules, resulting in optical emissions at 630, 557.7 and 427.8 nm. During O-mode transmissions at 5.423 MHz, using 630 MW effective radiated power, in the hours after sunset on 12 November 2001 several new observations were made, including: (1) The first high-latitude observation of an HF induced optical emission at 427.8 nm and (2) Optical rings being formed at HF on followed by their collapse into a central blob. Both discoveries remain unexplained with current theories
Import of cytochrome c into mitochondria
The import of cytochrome c into mitochondria can be resolved into a number of discrete steps. Here we report on the covalent attachment of heme to apocytochrome c by the enzyme cytochrome c heme lyase in mitochondria from Neurospora crassa.
A new method was developed to measure directly the linkage of heme to apocytochrome c. This method is independent of conformational changes in the protein accompanying heme attachment. Tryptic peptides of [35S]cysteine-labelled apocytochrome c, and of enzymatically formed holocytochrome c, were resolved by reverse-phase HPLC. The cysteine-containing peptide to which heme was attached eluted later than the corresponding peptide from apocytochrome c and could be quantified by counting 35S radioactivity as a measure of holocytochrome c formation. Using this procedure, the covalent attachment of heme to apocytochrome c, which is dependent on the enzyme cytochrome c heme lyase, could be measured. Activity required heme (as hemin) and could be reversibly inhibited by the analogue deuterohemin. Holocytochrome c formation was stimulated 5–10-fold by NADH > NADPH > glutathione and was independent of a potential across the inner mitochondrial membrane. NADH was not required for the binding of apocytochrome c to mitochondria and was not involved in the reduction of the cysteine thiols prior to heme attachment. Holocytochrome c formation was also dependent on a cytosolic factor that was necessary for the heme attaching step of cytochrome c import. The factor was a heat-stable, protease-insensitive, low-molecular-mass component of unknown function.
Cytochrome c heme lyase appeared to be a soluble protein located in the mitochondrial intermembrane space and was distinct from the previously identified apocytochrome c binding protein having a similar location. A model is presented in which the covalent attachment of heme by cytochrome c heme lyase also plays an essential role in the import pathway of cytochrome c
Quantum resistance metrology in graphene
We have performed a metrological characterization of the quantum Hall
resistance in a 1 m wide graphene Hall-bar. The longitudinal resistivity
in the center of the quantum Hall plateaus vanishes within the
measurement noise of 20 m upto 2 A. Our results show that the
quantization of these plateaus is within the experimental uncertainty (15 ppm
for 1.5A current) equal to that in conventional semiconductors. The
principal limitation of the present experiments are the relatively high contact
resistances in the quantum Hall regime, leading to a significantly increased
noise across the voltage contacts and a heating of the sample when a high
current is applied
The dynamical background of polar mesosphere winter echoes from simultaneous EISCAT and ESRAD observations
On 30 October 2004 during a strong solar proton event, layers of enhanced backscatter from altitudes between 55 and 75km have been observed by both ESRAD (52MHz) and the EISCAT VHF (224MHz) radars. These echoes have earlier been termed Polar Mesosphere Winter Echoes, PMWE. After considering the morphology of the layers and their relation to observed atmospheric waves, we conclude that the radars have likely seen the same phenomenon even though the radars' scattering volumes are located about 220km apart and that the most long-lasting layer is likely associated with wind-shear in an inertio-gravity wave. An ion-chemistry model is used to determine parameters necessary to relate wind-shear induced turbulent energy dissipation rates to radar backscatter. The model is verified by comparison with electron density profiles measured by the EISCAT VHF radar. Observed radar signal strengths are found to be 2-3 orders of magnitude stronger than the maximum which can be expected from neutral turbulence alone, assuming that previously published results relating radar signal scatter to turbulence parameters, and turbulence parameters to wind shear, are correct. The possibility remains that some additional or alternative mechanism may be involved in producing PMWE, such as layers of charged dust/smoke particles or large cluster ions
Structure and Thermodynamics of the Mixed Alkali Alanates
The thermodynamics and structural properties of the hexahydride alanates
(M2M'AlH6) with the elpasolite structure have been investigated. A series of
mixed alkali alanates (Na2LiAlH6, K2LiAlH6 and K2NaAlH6) were synthesized and
found to reversibly absorb and desorb hydrogen without the need for a catalyst.
Pressure-composition isotherms were measured to investigate the thermodynamics
of the absorption and desorption reactions with hydrogen. Isotherms for
catalyzed (4 mol% TiCl3) and uncatalyzed Na2LiAlH6 exhibited an increase in
kinetics, but no change in the bulk thermodynamics with the addition of a
dopant. A structural analysis using synchrotron x-ray diffraction showed that
these compounds favor the Fm-3m space group with the smaller ion (M') occupying
an octahedral site. These results demonstrate that appropriate cation
substitutions can be used to stabilize or destabilize the material and may
provide an avenue to improving the unfavorable thermodynamics of a number of
materials with promising gravimetric hydrogen densities.Comment: 6 pages, 7 figures,3 tables, submitted to PR
A comparison of overshoot modelling with observations of polar mesospheric summer echoes at radar frequencies of 56 and 224 MHz
We have compared radar observations of polar mesospheric summer echoes (PMSEs) modulated by artificial electron heating, at frequencies of 224 MHz (EISCAT VHF) and 56 MHz (MORRO). We have concentrated on 1 day of observation, lasting ∼ 3.8 h. The MORRO radar, with its much wider beam, observes one or more PMSE layers all the time while the VHF radar observes PMSEs in 69% of the time. Statistically there is a clear difference between how the MORRO and the VHF radar backscatter reacts to the heater cycling (48 s heater on and 168 s heater off). While MORRO often reacts by having its backscatter level increased when the heater is switched on, as predicted by Scales and Chen (2008), the VHF radar nearly always sees the "normal" VHF overshoot behaviour with an initial rapid reduction of backscatter. However, in some heater cycles we do see a substantial recovery of the VHF backscatter after its initial reduction to levels several times above that just before the heater was switched on. For the MORRO radar a recovery during the heater-on phase is much more common. The reaction when the heater was switched off was a clear overshoot for nearly all VHF cases but less so for MORRO. A comparison of individual curves for the backscatter values as a function of time shows, at least for this particular day, that in high layers above ∼ 85 km height, both radars see a reduction of the backscatter as the heater is switched on, with little recovery during the heater-on time. These variations are well described by present models. On the other hand, the backscatter in low layers at 81-82 km can be quite different, with modest or no reduction in backscatter as the heater is switched on, followed by a strong recovery for both radars to levels several times above that of the undisturbed PMSEs. This simultaneous, nearly identical behaviour at the two very different radar frequencies is not well described by present modelling
Experimental And Topological Determination of the Pressure-Temperature Phase Diagram of Racemic Etifoxine, a Pharmaceutical Ingredient with Anxyolitic Properties
International audienc
Peritoneal Protein Losses and Cytokine Generation in Automated Peritoneal Dialysis with Combined Amino Acids and Glucose Solutions
Objectives. Protein-energy malnutrition as a consequence of deficient protein intake frequently occurs in peritoneal dialysis (PD) patients. Previously, we showed that peritoneal dialysate containing a mixture of amino acids (AA) and glucose has anabolic effects. However AA-dialysate has been reported to increase intraperitoneal protein and AA losses and the release of proinflammatory cytokines (interleukine-6 (IL-6) and tumor necrosis factor alpha (TNFα)). We investigated the effect
of AA plus glucose (AAG) solutions on peritoneal protein losses and cytokine generation.
Methods. In 6 patients on standard automated peritoneal dialysis (APD) 12 APD sessions of 6 cycles each were performed during the night using dialysate containing 1.1% AA plus glucose or glucose alone as control. Protein losses and TNFα and IL-6 concentrations were measured in dialysates separately collected from nightly
cycling and daytime dwell. Results. The 24 hour-protein losses with AAG (median 6.7 g, range 4.7–9.4 g) were similar
to control dialysate (median 6.0 g, range 4.2–9.2 g). Daytime dialysate IL-6 levels were higher after nightly AAG
dialysis than after control dialysis (142 pg/ml and 82 pg/ml, respectively, P<.05). TNFα concentrations were very low.
Conclusion. Nightly APD with amino acids containing dialysate was associated with an increase in
peritoneal IL-6 generation during the day. The addition of AA to standard glucose dialysis solutions did not induce
a significant increase of peritoneal protein losses
Ice XII in its second regime of metastability
We present neutron powder diffraction results which give unambiguous evidence
for the formation of the recently identified new crystalline ice phase[Lobban
et al.,Nature, 391, 268, (1998)], labeled ice XII, at completely different
conditions. Ice XII is produced here by compressing hexagonal ice I_h at T =
77, 100, 140 and 160 K up to 1.8 GPa. It can be maintained at ambient pressure
in the temperature range 1.5 < T < 135 K. High resolution diffraction is
carried out at T = 1.5 K and ambient pressure on ice XII and accurate
structural properties are obtained from Rietveld refinement. At T = 140 and 160
K additionally ice III/IX is formed. The increasing amount of ice III/IX with
increasing temperature gives an upper limit of T ~ 150 K for the successful
formation of ice XII with the presented procedure.Comment: 3 Pages of RevTeX, 3 tables, 3 figures (submitted to Physical Review
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