PENGARUH MODEL PEMBELAJARAN KOOPERATIF TIPE THINK TALK WRITE TERHADAP KEMAMPUAN KOMUNIKASI MATEMATIS

This quasi experimental research aimed to find out the influence of cooperative learning model of think talk write type towards students mathematical communication skill. The design which was used waspretest posttest control group design. The population of this research was all students of grade 7thof SMP Negeri 3 Bandar Lampung in academic year of 2015/2016 that was distributed into eight classes. The samples of this research were students of VII-H and VII-I class which were taken by purposive sampling technique. The research data were obtained by test.The conclusion of this research wasthe cooperative learning of Think Talk Write affect towards the students mathematical communication skill.Penelitian eksperimen semu ini bertujuan untuk mengetahui pengaruh model pembelajaran kooperatif tipe think talk write terhadap kemampuan komunikasi matematis siswa. Desain yang digunakan adalah pretest posttest control group design. Populasi penelitian ini adalah seluruh siswa kelas VII SMP Negeri 3 Bandar Lampng tahun pelajaran 2015/2016 yang terdistribusi dalam delapan kelas. Sampel penelitian ini adalah siswa kelas VII-H dan VII-I yang diambil dengan teknik purposive sampling. Data penelitian diperoleh melalui tes. Kesimpulan penelitian ini adalah pembelajaran kooperatif tipe Think Talk Write berpengaruh terhadap kemampuan komunikasi matematis siswa.Kata kunci: kemampuan komunikasi, pengaruh, think talk writ

PENGUKURAN INTENSITAS DAN PENETRASI CAHAYA LAMPU LED (LIGHT EMITTING DIODE) BAWAH AIR DAN TL (TUBULAR LAMP) PADA BAGAN RAKIT

Penelitian pengukuran sebaran intensitas pada lampu TL dan lampu LED bawah air pada bagan rakit telah dilakukan di perairan Palabuhanratu. Tujuan penelitian mengukur iluminasi dan sebaran cahaya lampu LED 60 watt, TL 390 dan 585 watt serta mengidentifikasi hasil tangkapan. Metode yang digunakan dalam penelitian ini adalah survei dengan mengikuti kegiatan langsung operasi penangkapan ikan. Hasil penelitian menunjukkan bahwa penetrasi cahaya lampu LED 60 watt mampu menembus perairan hingga kedalaman 9 meter. Nilai tertinggi terdapat pada titik 0, kedalaman 1 meter dengan nilai 2,5x10-4 watt/cm2 dan titik terendah terdapat pada titik 1 pada kedalaman 9 meter dengan nilai 3E-005 watt/cm2. Penetrasi cahaya kedua lampu TL yaitu TL 390 dan 585 watt hanya mampu menembus air hingga kedalaman 3-4 meter. Pengukuran lampu TL 390 watt didapatkan nilai tertinggi pada titik 0 di kedalaman 0 meter dengan nilai 0,358 watt/cm2. Pengukuran lampu TL 585 watt didapatkan nilai tertinggi pada titik 0 di kedalaman 1 meter dengan nilai 0.819 watt/cm2. Hasil tangkapan LED didominasi oleh ikan petek sebesar 94%, lampu TL 390 dan TL 585 watt didominasi ikan teri (Stolephorus sp) sebesar 55% dan 49%. Kata kunci: bagan rakit, lampu LED, lampu TLResearch on measuring the intensity distribution of TL lamps and underwater LED lights on bagan carried out in Pelabuhanratu waters. The purpose of the study was to measure the illumination and the light distribution of 60 watt LED lights, TL 390 and 585 watts and to identify the catch. The method used in this study is a survey method. The results showed that the light penetration of a 60 watt LED lamp was able to penetrate the waters to a depth of 9 meters. The highest value found at point 1 depth of 1 meter with a value of 2.5x10-4 watt / cm2 and the lowest point is at point 1 at a depth of 9 meters with a value of 3E-005 watts / cm 2. The light penetration of both TL lamps, TL 390 and 585 watts, is only able to penetrate water to a depth of 3-4 meters. TL 390 watt lamp measurements obtained the highest value at point 0 at a depth of 0 meters with a value of 0.358 watt / cm2. The measurement of TL 585 watt lights obtained the highest value at point 0 at a depth of 1 meter with a value of 0.819 watts/cm2. The catch of LED was dominated by petek 94% (Leiognathus dussumieri) while TL 390 lights and TL 585 was dominated by teri (Stolephorus sp.) 55% and 49% respectively. Key words: lifnet, LED lamp, TL lam

The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants

CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like gene regulation. However, the design and assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning and is still troublesome due to the presence of repetitive sequences, thus hampering a more widespread use. Here we present a comprehensive extension of the software-assisted cloning platform GoldenBraid (GB), in which, on top of its multigene cloning software, we integrate new tools for the Type IIS-based easy and rapid assembly of up to six tandemly-arrayed gRNAs with both Cas9 and Cas12a, using the gRNA-tRNA-spaced and the crRNA unspaced approaches, respectively. As stress tests for the new tools, we assembled and used for Agrobacterium-mediated stable transformation a 17 Cas9-gRNAs construct targeting a subset of the Squamosa-Promoter Binding Protein-Like (SPL) gene family in Nicotiana tabacum. The 14 selected genes are targets of miR156, thus potentially playing an important role in juvenile-to-adult and vegetative-to-reproductive phase transitions. With the 17 gRNAs construct we generated a collection of Cas9-free SPL edited T plants harboring up to 9 biallelic mutations and showing leaf juvenility and more branching. The functionality of GB-assembled dCas9 and dCas12a-based CRISPR/Cas activators and repressors using single and multiplexing gRNAs was validated using a Luciferase reporter with the Solanum lycopersicum Mtb promoter or the Agrobacterium tumefaciens nopaline synthase promoter in transient expression in Nicotiana benthamiana. With the incorporation of the new web-based tools and the accompanying collection of DNA parts, the GB4.0 genome edition turns an all-in-one open platform for plant genome engineering

The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants

[EN] CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like gene regulation. However, the design and assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning and is still troublesome due to the presence of repetitive sequences, thus hampering a more widespread use. Here we present a comprehensive extension of the software-assisted cloning platform GoldenBraid (GB), in which, on top of its multigene cloning software, we integrate new tools for the Type IIS-based easy and rapid assembly of up to six tandemly-arrayed gRNAs with both Cas9 and Cas12a, using the gRNA-tRNA-spaced and the crRNA unspaced approaches, respectively. As stress tests for the new tools, we assembled and used for Agrobacterium-mediated stable transformation a 17 Cas9-gRNAs construct targeting a subset of the Squamosa-Promoter Binding Protein-Like (SPL) gene family in Nicotiana tabacum. The 14 selected genes are targets of miR156, thus potentially playing an important role in juvenile-to-adult and vegetative-to-reproductive phase transitions. With the 17 gRNAs construct we generated a collection of Cas9-free SPL edited T-1 plants harboring up to 9 biallelic mutations and showing leaf juvenility and more branching. The functionality of GB-assembled dCas9 and dCas12a-based CRISPR/Cas activators and repressors using single and multiplexing gRNAs was validated using a Luciferase reporter with the Solanum lycopersicum Mtb promoter or the Agrobacterium tumefaciens nopaline synthase promoter in transient expression in Nicotiana benthamiana. With the incorporation of the new web-based tools and the accompanying collection of DNA parts, the GB4.0 genome edition turns an all-in-one open platform for plant genome engineering.This work had been funded by EU Horizon 2020 Project Newcotiana Grant 760331 and PID2019-108203RB-100 Plan Nacional I+D, Spanish Ministry of Economy and Competitiveness. MV-V was recipient of aGeneralitat Valenciana and Fondo Social Europeo post-doctoral grant. JB-O and SS were recipients of FPI fellowships. CP was recipient of a Santiago Grisolia fellowship (Generalitat Valenciana).Vazquez-Vilar, M.; Garcia-Carpintero, V.; Selma, S.; Bernabé-Orts, JM.; Sánchez-Vicente, J.; Salazar-Sarasua, B.; Ressa, A.... (2021). The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants. Frontiers in Plant Science. 12:1-14. https://doi.org/10.3389/fpls.2021.6899371141

PENGEMBANGAN DESA WISATA TERENCANA DI DESA HURUNG BUNUT, KECAMATAN KURUN, KABUPATEN GUNUNG MAS

Desa wisata merupakan desa yang dijadikan tempat wisata karena daya tarik yang dimilikinya. Potensi pariwisata Desa Hurung Bunut seperti susur sungai, sanggar tari, budidaya madu kelulut dan memiliki keindahan alam yang sangat indah. Dengan banyaknya potensi wisata yang ada di Desa Hurung Bunut sangatlah cocok dijadikan sebagai desa wisata. Di Desa Hurung Bunut sudah mendapatkan SK Desa Wisata dari Bupati Kabupaten Gunung Mas. Dengan melihat potensi wisata yang ada di Desa Hurung Bunut, kami kelompok KKN-T Mandiri Desa Hurung Bunut menawarkan untuk membuat spot foto di bukit alam dan wisata petik madu kelulut. Target capaian yang diharapkan KKN-T Mandiri Desa Hurung Bunut ini adalah untuk mewujudkan desa wisata di Desa Hurung Bunut, sehingga dapat meningkatkan pendapatan masyarakat sekitar dalam rangka mempercepat pemulihan ekonomi dan sosia

Measurement of the 2νββ\nu\beta\beta Decay Half-Life of Se-82 with the Global CUPID-0 Background Model

We report on the results obtained with the global CUPID-0 background model, which combines the data collected in the two measurement campaigns for a total exposure of 8.82~kg$\times$yr of $^{82}$Se. We identify with improved precision the background sources within the 3 MeV energy region, where neutrinoless double $\beta$-decay of $^{82}$Se and $^{100}$Mo is expected, making more solid the foundations for the background budget of the next-generation CUPID experiment. Relying on the excellent data reconstruction, we measure the two-neutrino double $\beta$-decay half-life of $^{82}$Se with unprecedented accuracy: $T_{1/2}^{2\nu} = [8.69 \pm 0.05 \textrm{(stat.)}~^{+0.09}_{-0.06} \textrm{(syst.)}] \times 10^{19}~\textrm{yr}$

Search for Majoron-like particles with CUPID-0

We present the first search for the Majoron-emitting modes of the neutrinoless double $\beta$ decay ($0\nu\beta\beta\chi_0$) using scintillating cryogenic calorimeters. We analysed the CUPID-0 Phase I data using a Bayesian approach to reconstruct the background sources activities, and evaluate the potential contribution of the $^{82}$Se $0\nu\beta\beta\chi_0$. We considered several possible theoretical models which predict the existence of a Majoron-like boson coupling to the neutrino. The energy spectra arising from the emission of such bosons in the neutrinoless double $\beta$ decay have spectral indices $n=$ 1, 2, 3 or 7. We found no evidence of any of these decay modes, setting a lower limit (90% of credibility interval) on the half-life of 1.2 $\times$ 10$^{23}$ yr in the case of $n=$ 1, 3.8 $\times$ 10$^{22}$ yr for $n=$ 2, 1.4 $\times$ 10$^{22}$ yr for $n=$ 3 and 2.2 $\times$ 10$^{21}$ yr for $n=$ 7. These are the best limits on the $0\nu\beta\beta\chi_0$ half-life of the $^{82}$Se, and demonstrate the potentiality of the CUPID-0 technology in this field

Status and prospects of discovery of 0νββ decay with the CUORE detector

In this contribution we present the achievements of the CUORE experiment so far. It is the first tonne-scale bolometric detector and it is in stable data taking since 2018. We reached to collect about 1800 kg×yr of exposure of which more than 1ton×year have been analysed. The CUORE detector is meant to search for the neutrinoless double β decay (0νββ) of the 130Te isotope. This is a beyond Standard Model process which could establish the nature of the neutrino to be Dirac or a Majorana particle. It is an alternative mode of the two-neutrinos double β decay, a rare decay which have been precisely measured by CUORE in the 130Te. We found no evidence of the 0νββ and we set a Bayesian lower limit of 2.2×1025yr on its half-life. The expertise achieved by CUORE set a milestone for any future bolometric detector, including CUPID, which is the planned next generation experiment searching for 0νββ with scintillating bolometers

Twelve-crystal prototype of Li2_2MoO4_4 scintillating bolometers for CUPID and CROSS experiments

An array of twelve 0.28 kg lithium molybdate (LMO) low-temperature bolometers equipped with 16 bolometric Ge light detectors, aiming at optimization of detector structure for CROSS and CUPID double-beta decay experiments, was constructed and tested in a low-background pulse-tube-based cryostat at the Canfranc underground laboratory in Spain. Performance of the scintillating bolometers was studied depending on the size of phonon NTD-Ge sensors glued to both LMO and Ge absorbers, shape of the Ge light detectors (circular vs. square, from two suppliers), in different light collection conditions (with and without reflector, with aluminum coated LMO crystal surface). The scintillating bolometer array was operated over 8 months in the low-background conditions that allowed to probe a very low, $\mu$Bq/kg, level of the LMO crystals radioactive contamination by $^{228}$Th and $^{226}$Ra.Comment: Prepared for submission to JINST; 23 pages, 9 figures, and 4 table

A CUPID Li2100MoO4scintillating bolometer tested in the CROSS underground facility

A scintillating bolometer based on a large cubic Li2100MoO4 crystal (45 mm side) and a Ge wafer (scintillation detector) has been operated in the CROSS cryogenic facility at the Canfranc underground laboratory in Spain. The dual-readout detector is a prototype of the technology that will be used in the next-generation 0¿2ß experiment CUPID . The measurements were performed at 18 and 12 mK temperature in a pulse tube dilution refrigerator. This setup utilizes the same technology as the CUORE cryostat that will host CUPID and so represents an accurate estimation of the expected performance. The Li2100MoO4 bolometer shows a high energy resolution of 6 keV FWHM at the 2615 keV ¿ line. The detection of scintillation light for each event triggered by the Li2100MoO4 bolometer allowed for a full separation (~8s) between ¿(ß) and a events above 2 MeV . The Li2100MoO4 crystal also shows a high internal radiopurity with 228Th and 226Ra activities of less than 3 and 8 µBq/kg, respectively. Taking also into account the advantage of a more compact and massive detector array, which can be made of cubic-shaped crystals (compared to the cylindrical ones), this test demonstrates the great potential of cubic Li2100MoO4 scintillating bolometers for high-sensitivity searches for the 100Mo 0¿2ß decay in CROSS and CUPID projects