MRI role in the periprosthetic lymphoma screening: a case report

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THE STUDY COMPARISON OF METACOGNITIVE ABILITY BETWEEN INSTAD COMBINED CONCEPT MAP AND CONVENTIONAL LEARNING OF BIOLOGY LEARNING X GRADE STUDENTS AT SMA NEGERI 1 SUKOHARJO IN ACADEMIC YEAR 2012/2013

The aim of this research was to ascertain metacognitive ability differences between instad combined with concept map and conventional learning of biology learning X grade students SMA Negeri 1 Sukoharjo in academic year 2012/2013.            This research was quasy experiment method. This research design was Post-Test Only with Nonequivalent Control Group Design. The population of this research was all of X grade students at SMA Negeri 1 Sukoharjo in academic year 2012/2013 with 255 students. Sampling techniques used cluster sampling. The sample of this research was class X1 as experiment class with 32 students and class X2 as comparison class with 31 students. The data collected using essay test, observations and school document. The hypotheses was analyzed by t-test.The result of this research showed that t-test of metacognitive ability was significance < 0,05 is 0,015. The average value metacognitive ability of experiment class was 80,85 and comparisan class was 75,94.  The average value metacognitive ability of experiment class was higher than the comparison class.            The research concluded that there was metacognitive ability differences between INSTAD combined with concept map and conventional learning of biology learning grade X student SMA Negeri 1 Sukoharjo in academic year 2012/2013. Keyword: INSTAD, Concept map, Metacognitive ability

The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants

CRISPR/Cas ability to target several loci simultaneously (multiplexing) is a game-changer in plant breeding. Multiplexing not only accelerates trait pyramiding but also can unveil traits hidden by functional redundancy. Furthermore, multiplexing enhances dCas-based programmable gene expression and enables cascade-like gene regulation. However, the design and assembly of multiplex constructs comprising tandemly arrayed guide RNAs (gRNAs) requires scarless cloning and is still troublesome due to the presence of repetitive sequences, thus hampering a more widespread use. Here we present a comprehensive extension of the software-assisted cloning platform GoldenBraid (GB), in which, on top of its multigene cloning software, we integrate new tools for the Type IIS-based easy and rapid assembly of up to six tandemly-arrayed gRNAs with both Cas9 and Cas12a, using the gRNA-tRNA-spaced and the crRNA unspaced approaches, respectively. As stress tests for the new tools, we assembled and used for Agrobacterium-mediated stable transformation a 17 Cas9-gRNAs construct targeting a subset of the Squamosa-Promoter Binding Protein-Like (SPL) gene family in Nicotiana tabacum. The 14 selected genes are targets of miR156, thus potentially playing an important role in juvenile-to-adult and vegetative-to-reproductive phase transitions. With the 17 gRNAs construct we generated a collection of Cas9-free SPL edited T plants harboring up to 9 biallelic mutations and showing leaf juvenility and more branching. The functionality of GB-assembled dCas9 and dCas12a-based CRISPR/Cas activators and repressors using single and multiplexing gRNAs was validated using a Luciferase reporter with the Solanum lycopersicum Mtb promoter or the Agrobacterium tumefaciens nopaline synthase promoter in transient expression in Nicotiana benthamiana. With the incorporation of the new web-based tools and the accompanying collection of DNA parts, the GB4.0 genome edition turns an all-in-one open platform for plant genome engineering

Measurement of the 2νββ\nu\beta\beta Decay Half-Life of Se-82 with the Global CUPID-0 Background Model

We report on the results obtained with the global CUPID-0 background model, which combines the data collected in the two measurement campaigns for a total exposure of 8.82~kg$\times$yr of $^{82}$Se. We identify with improved precision the background sources within the 3 MeV energy region, where neutrinoless double $\beta$-decay of $^{82}$Se and $^{100}$Mo is expected, making more solid the foundations for the background budget of the next-generation CUPID experiment. Relying on the excellent data reconstruction, we measure the two-neutrino double $\beta$-decay half-life of $^{82}$Se with unprecedented accuracy: $T_{1/2}^{2\nu} = [8.69 \pm 0.05 \textrm{(stat.)}~^{+0.09}_{-0.06} \textrm{(syst.)}] \times 10^{19}~\textrm{yr}$

Search for Majoron-like particles with CUPID-0

We present the first search for the Majoron-emitting modes of the neutrinoless double $\beta$ decay ($0\nu\beta\beta\chi_0$) using scintillating cryogenic calorimeters. We analysed the CUPID-0 Phase I data using a Bayesian approach to reconstruct the background sources activities, and evaluate the potential contribution of the $^{82}$Se $0\nu\beta\beta\chi_0$. We considered several possible theoretical models which predict the existence of a Majoron-like boson coupling to the neutrino. The energy spectra arising from the emission of such bosons in the neutrinoless double $\beta$ decay have spectral indices $n=$ 1, 2, 3 or 7. We found no evidence of any of these decay modes, setting a lower limit (90% of credibility interval) on the half-life of 1.2 $\times$ 10$^{23}$ yr in the case of $n=$ 1, 3.8 $\times$ 10$^{22}$ yr for $n=$ 2, 1.4 $\times$ 10$^{22}$ yr for $n=$ 3 and 2.2 $\times$ 10$^{21}$ yr for $n=$ 7. These are the best limits on the $0\nu\beta\beta\chi_0$ half-life of the $^{82}$Se, and demonstrate the potentiality of the CUPID-0 technology in this field

Twelve-crystal prototype of Li2_2MoO4_4 scintillating bolometers for CUPID and CROSS experiments

An array of twelve 0.28 kg lithium molybdate (LMO) low-temperature bolometers equipped with 16 bolometric Ge light detectors, aiming at optimization of detector structure for CROSS and CUPID double-beta decay experiments, was constructed and tested in a low-background pulse-tube-based cryostat at the Canfranc underground laboratory in Spain. Performance of the scintillating bolometers was studied depending on the size of phonon NTD-Ge sensors glued to both LMO and Ge absorbers, shape of the Ge light detectors (circular vs. square, from two suppliers), in different light collection conditions (with and without reflector, with aluminum coated LMO crystal surface). The scintillating bolometer array was operated over 8 months in the low-background conditions that allowed to probe a very low, $\mu$Bq/kg, level of the LMO crystals radioactive contamination by $^{228}$Th and $^{226}$Ra.Comment: Prepared for submission to JINST; 23 pages, 9 figures, and 4 table

Status and prospects of discovery of 0νββ decay with the CUORE detector

In this contribution we present the achievements of the CUORE experiment so far. It is the first tonne-scale bolometric detector and it is in stable data taking since 2018. We reached to collect about 1800 kg×yr of exposure of which more than 1ton×year have been analysed. The CUORE detector is meant to search for the neutrinoless double β decay (0νββ) of the 130Te isotope. This is a beyond Standard Model process which could establish the nature of the neutrino to be Dirac or a Majorana particle. It is an alternative mode of the two-neutrinos double β decay, a rare decay which have been precisely measured by CUORE in the 130Te. We found no evidence of the 0νββ and we set a Bayesian lower limit of 2.2×1025yr on its half-life. The expertise achieved by CUORE set a milestone for any future bolometric detector, including CUPID, which is the planned next generation experiment searching for 0νββ with scintillating bolometers

A CUPID Li2100MoO4scintillating bolometer tested in the CROSS underground facility

A scintillating bolometer based on a large cubic Li2100MoO4 crystal (45 mm side) and a Ge wafer (scintillation detector) has been operated in the CROSS cryogenic facility at the Canfranc underground laboratory in Spain. The dual-readout detector is a prototype of the technology that will be used in the next-generation 0¿2ß experiment CUPID . The measurements were performed at 18 and 12 mK temperature in a pulse tube dilution refrigerator. This setup utilizes the same technology as the CUORE cryostat that will host CUPID and so represents an accurate estimation of the expected performance. The Li2100MoO4 bolometer shows a high energy resolution of 6 keV FWHM at the 2615 keV ¿ line. The detection of scintillation light for each event triggered by the Li2100MoO4 bolometer allowed for a full separation (~8s) between ¿(ß) and a events above 2 MeV . The Li2100MoO4 crystal also shows a high internal radiopurity with 228Th and 226Ra activities of less than 3 and 8 µBq/kg, respectively. Taking also into account the advantage of a more compact and massive detector array, which can be made of cubic-shaped crystals (compared to the cylindrical ones), this test demonstrates the great potential of cubic Li2100MoO4 scintillating bolometers for high-sensitivity searches for the 100Mo 0¿2ß decay in CROSS and CUPID projects

A first test of CUPID prototypal light detectors with NTD-Ge sensors in a pulse-tube cryostat

CUPID is a next-generation bolometric experiment aiming at searching for neutrinoless double-beta decay with ~250 kg of isotopic mass of $^{100}$Mo. It will operate at $\sim$10 mK in a cryostat currently hosting a similar-scale bolometric array for the CUORE experiment at the Gran Sasso National Laboratory (Italy). CUPID will be based on large-volume scintillating bolometers consisting of $^{100}$Mo-enriched Li$_2$MoO$_4$ crystals, facing thin Ge-wafer-based bolometric light detectors. In the CUPID design, the detector structure is novel and needs to be validated. In particular, the CUORE cryostat presents a high level of mechanical vibrations due to the use of pulse tubes and the effect of vibrations on the detector performance must be investigated. In this paper we report the first test of the CUPID-design bolometric light detectors with NTD-Ge sensors in a dilution refrigerator equipped with a pulse tube in an above-ground lab. Light detectors are characterized in terms of sensitivity, energy resolution, pulse time constants, and noise power spectrum. Despite the challenging noisy environment due to pulse-tube-induced vibrations, we demonstrate that all the four tested light detectors comply with the CUPID goal in terms of intrinsic energy resolution of 100 eV RMS baseline noise. Indeed, we have measured 70--90 eV RMS for the four devices, which show an excellent reproducibility. We have also obtained outstanding energy resolutions at the 356 keV line from a $^{133}$Ba source with one light detector achieving 0.71(5) keV FWHM, which is -- to our knowledge -- the best ever obtained when compared to $\gamma$ detectors of any technology in this energy range.Comment: Prepared for submission to JINST; 16 pages, 7 figures, and 1 tabl