El «modelo» norteamericano en la reglamentación de las intervenciones federales en la Argentina decimonónica. Debates en el Congreso Nacional (1869 y 1894)

This paper studies the role played by American Constitutional Law as doctrinal reference during legislative and public debates on the regulation of the national government’s powers to intervene in provincial affairs in 19th century Argentina.Este trabajo examina el lugar de la legislación de los Estados Unidos como referencia doctrinal en los dos mayores debates legislativos de la Argentina decimonónica acerca de la reglamentación de las facultades del gobierno nacional para intervenir en las provincias

UK guideline on transition of adolescent and young persons with chronic digestive diseases from paediatric to adult care

The risks of poor transition include delayed and inappropriate transfer that can result in disengagement with healthcare. Structured transition care can improve control of chronic digestive diseases and long-term health-related outcomes. These are the first nationally developed guidelines on the transition of adolescent and young persons (AYP) with chronic digestive diseases from paediatric to adult care. They were commissioned by the Clinical Services and Standards Committee of the British Society of Gastroenterology under the auspices of the Adolescent and Young Persons (A&YP) Section. Electronic searches for English-language articles were performed with keywords relating to digestive system diseases and transition to adult care in the Medline (via Ovid), PsycInfo (via Ovid), Web of Science and CINAHL databases for studies published from 1980 to September 2014. The quality of evidence and grading of recommendations was appraised using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system. The limited number of studies in gastroenterology and hepatology required the addition of relevant studies from other chronic diseases to be included. These guidelines deal specifically with the transition of AYP living with a diagnosis of chronic digestive disease and/or liver disease from paediatric to adult healthcare under the following headings; 1. Patient populations involved in AYP transition 2. Risks of failing transition or poor transition 3. Models of AYP transition 4. Patient and carer/parent perspective in AYP transition 5. Surgical perspectiv

Determination of protein structures by two dimensional vibrational spectroscopy isotope dilution experiments

Prior to this work two-dimensional vibrational echo spectroscopy (2D-IR) had been limited to the determination of small peptide structures. This thesis proposes and demonstrates a methodology that allows the extension of the spectroscopic principles gained with small molecules to the determination of structures of more complex systems, in particular, transmembrane helix dimers. Micelle suspensions of pairs of transmembrane domains are prepared with varying levels of heavy (13C=18O) isotope substitutions in the helix peptide backbone at selected locations. Vibrational coupling causes tertiary delocalization of the vibrational excitation and a splitting of the isotopic pair spectral transitions. The ultrafast time resolution of 2D-IR allows one to determine the vibrational frequency correlation of the selected modes and establish a dynamical model of spectra that is adequate to extract vibrational coupling constants from the comparison of spectra at different isotopic concentrations. The frequency correlation function is also capable of indicating the presence of mobile water associated with the labeled residues. The constraints derived from vibrational coupling of the precisely spaced heavy residues leads to determination of an optimized structure from a range of model candidates. The methods developed herein are applicable and transferable to other protein samples. This approach, as described, will identify conformational exchange or any other dynamical phenomena in addition to characterizing the structure of the exchanging species in picoseconds (ps) or longer timescales

Determination of protein structures by two dimensional vibrational spectroscopy isotope dilution experiments

Prior to this work two-dimensional vibrational echo spectroscopy (2D-IR) had been limited to the determination of small peptide structures. This thesis proposes and demonstrates a methodology that allows the extension of the spectroscopic principles gained with small molecules to the determination of structures of more complex systems, in particular, transmembrane helix dimers. Micelle suspensions of pairs of transmembrane domains are prepared with varying levels of heavy (13C=18O) isotope substitutions in the helix peptide backbone at selected locations. Vibrational coupling causes tertiary delocalization of the vibrational excitation and a splitting of the isotopic pair spectral transitions. The ultrafast time resolution of 2D-IR allows one to determine the vibrational frequency correlation of the selected modes and establish a dynamical model of spectra that is adequate to extract vibrational coupling constants from the comparison of spectra at different isotopic concentrations. The frequency correlation function is also capable of indicating the presence of mobile water associated with the labeled residues. The constraints derived from vibrational coupling of the precisely spaced heavy residues leads to determination of an optimized structure from a range of model candidates. The methods developed herein are applicable and transferable to other protein samples. This approach, as described, will identify conformational exchange or any other dynamical phenomena in addition to characterizing the structure of the exchanging species in picoseconds (ps) or longer timescales

Luminescence Quenching of Eu(III) Carboxylates by Cu(II) in a Composite Polymer Xerogel Film

Three Eu(III) luminescent compounds were separately entrapped in a xerogel porous silica matrix and finely ground particles of it were deposited on a glass support with polyvinylacetate (PVAc) as a binder to build a thin film sensor. These 3 devices were immersed in aqueous solutions of Cu(II) and the content of this metal was evaluated by emissionquenching experiments. The sensor containing the highly luminescent antenna chelate of diethylenetriaminepentaacetic acid (dtpa) sensitized with Coumarin120 rendered the largest Stern-Volmer constant (KSV 5 1.49 3 104 M21 ), showing no leaching of the Eu(III) complex to the aqueous solution and a reproducible value of the luminescence ratio between water and Cu(II) solution. The in situ sensor we developed can measure the concentration of Cu(II) in aqueous media down to the ppm level by emission-quenching experiments. This methodology permits a simple calibration of the sensor and an easy to use reusable device.Fil: Barja, Beatriz Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; ArgentinaFil: Remorino, Amanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Aramendia, Pedro Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentin