187 research outputs found
Modelling avalanches in martensites
Solids subject to continuous changes of temperature or mechanical load often
exhibit discontinuous avalanche-like responses. For instance, avalanche
dynamics have been observed during plastic deformation, fracture, domain
switching in ferroic materials or martensitic transformations. The statistical
analysis of avalanches reveals a very complex scenario with a distinctive lack
of characteristic scales. Much effort has been devoted in the last decades to
understand the origin and ubiquity of scale-free behaviour in solids and many
other systems. This chapter reviews some efforts to understand the
characteristics of avalanches in martensites through mathematical modelling.Comment: Chapter in the book "Avalanches in Functional Materials and
Geophysics", edited by E. K. H. Salje, A. Saxena, and A. Planes. The final
publication is available at Springer via
http://dx.doi.org/10.1007/978-3-319-45612-6_
Expression and characterization of the Trypanosoma cruzi dihydrofolate reductase domain
We have cloned and expressed in Escherichia coli a 702-base pair gene coding for the dihydrofolate reductase (DHFR) domain of the bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Trypanosoma cruzi. The DHFR domain was purified to homogeneity by methotrexate-Sepharose chromatography followed by an anion-exchange chromatography step in a mono Q column, and displayed a single 27-kDa band on SDS-PAGE. Gel filtration showed that the catalytic domain was expressed as a monomer. Kinetic parameters were similar to those reported for the wild-type bifunctional enzyme with Km values of 0.75 microM for dihydrofolate and 16 microM for NADPH and a kcat value of 16.5 s-1. T. cruzi DHFR is poorly inhibited by trimethoprim and pyrimethamine and the inhibition constants were always lower for the bifunctional enzyme. The binding of methotrexate was characteristic of a class of inhibitors that form an initial complex which isomerizes slowly to a tighter complex and are referred to as 'slow, tight-binding' inhibitors. While the slow-binding step of inhibition was apparently unaffected in the individually expressed DHFR domain, the overall inhibition constant was two-fold higher as a consequence of the superior inhibition constant value obtained for the initial inhibitory complex
The use of cosmic muons in detecting heterogeneities in large volumes
The muon intensity attenuation method to detect heterogeneities in large
matter volumes is analyzed. Approximate analytical expressions to estimate the
collection time and the signal to noise ratio, are proposed and validated by
Monte Carlo simulations. Important parameters, including point spread function
and coordinate reconstruction uncertainty are also estimated using Monte Carlo
simulations.Comment: 8 pages, 11 figures, submetted to NIM
Field-Induced Magnetization Steps in Intermetallic Compounds and Manganese Oxides: The Martensitic Scenario
Field-induced magnetization jumps with similar characteristics are observed
at low temperature for the intermetallic germanide Gd5Ge4and the mixed-valent
manganite Pr0.6Ca0.4Mn0.96Ga0.04O3. We report that the field location -and even
the existence- of these jumps depends critically on the magnetic field sweep
rate used to record the data. It is proposed that, for both compounds, the
martensitic character of their antiferromagnetic-to-ferromagnetic transitions
is at the origin of the magnetization steps.Comment: 4 pages,4 figure
On the athermal character of structural phase transitions
The significance of thermal fluctuations on nucleation in structural
first-order phase transitions has been examined. The prototype case of
martensitic transitions has been experimentally investigated by means of
acoustic emission techniques. We propose a model based on the mean
first-passage time to account for the experimental observations. Our study
provides a unified framework to establish the conditions for isothermal and
athermal transitions to be observed.Comment: 5 pages, 4 figures, accepted in Phys. Rev. Let
Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi
We have cloned, sequenced and expressed the Trypanosoma cruzi gene encoding the bifunctional protein dihydrofolate reductase-thymidylate synthase (DHFR-TS). The strategy followed for the isolation of positive clones from a genomic library was based on the construction of a probe by the amplification of highly conserved sequences of the TS domain by the polymerase chain reaction. Translation of the open reading frame of 1563 bp yields a polypeptide of 521 amino acids with a molecular mass of 58829 Da. For heterologous expression of T. cruzi DHFR-TS in Escherichia coli, the entire coding sequence was amplified by polymerase chain reaction and cloned into the plasmid vector pKK223.3. The presence of catalytically active DHFR-TS was demonstrated by complementation of the Thy- E. coli strain chi 2913 and the DHFR- Thy- E. coli strain PA414. The gene is expressed as an active protein which constitutes approximately 2% of the total cell soluble protein. Recombinant bifunctional enzyme and the DHFR domain have been purified by methotrexate-Sepharose chromatography to yield 1-2 mg of active DHFR-TS per litre of culture. Southern and electrophoretic analyses using the coding sequence as probe indicated that the T. cruzi enzyme is encoded by a single copy gene which maps to two bands of approximately 990 kb and 1047 kb. It appears that T. cruzi is diploid for the DHFR-TS gene which is located on two different-sized homologous chromosomes
Fine Structure of Avalanches in the Abelian Sandpile Model
We study the two-dimensional Abelian Sandpile Model on a square lattice of
linear size L. We introduce the notion of avalanche's fine structure and
compare the behavior of avalanches and waves of toppling. We show that
according to the degree of complexity in the fine structure of avalanches,
which is a direct consequence of the intricate superposition of the boundaries
of successive waves, avalanches fall into two different categories. We propose
scaling ans\"{a}tz for these avalanche types and verify them numerically. We
find that while the first type of avalanches has a simple scaling behavior, the
second (complex) type is characterized by an avalanche-size dependent scaling
exponent. This provides a framework within which one can understand the failure
of a consistent scaling behavior in this model.Comment: 10 page
Hot exozodiacal dust resolved around Vega with IOTA/IONIC
Although debris discs have been detected around a significant number of
main-sequence stars, only a few of them are known to harbour hot dust in their
inner part where terrestrial planets may have formed. Thanks to infrared
interferometric observations, it is possible to obtain a direct measurement of
these regions, which are of prime importance for preparing future exo-Earth
characterisation missions. In this context, we have resolved the exozodiacal
dust disc around Vega with the help of infrared stellar interferometry and
estimated the integrated H-band flux originating from the first few AUs of the
debris disc. Using precise H-band interferometric measurements obtained with
the 3-telescope IOTA/IONIC interferometer (Mount Hopkins, Arizona), thorough
modelling of both interferometric data (squared visibility and closure phase)
and spectral energy distribution was performed to constrain the nature of the
near-infrared excess emission. The most straightforward scenario consists in a
compact dust disc producing a thermal emission that is largely dominated by
small grains located between 0.1 and 0.3 AU from Vega and accounting for 1.23
+/- 0.45% of the near-infrared stellar flux for our best-fit model. This flux
ratio is shown to vary slightly with the geometry of the model used to fit our
interferometric data (variations within +/-0.19%). Initially revealed by K-band
CHARA/FLUOR observations, the presence of hot exozodiacal dust in the vicinity
of Vega is confirmed by our H-band IOTA/IONIC measurements at the 3-sigma
level. Whereas the origin of the dust is still uncertain, its presence and the
possible connection with the outer disc suggest that the Vega system is
currently undergoing major dynamical perturbations.Comment: 10 pages, 9 figures, accepted for publication in A&
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