EDUKASI PEMELIHARAAN KESEHATAN GIGI DAN MULUT PADA MURID DI SDN 23/IX DAN 56/IX DESA PONDOK MEJA KABUPATEN MUARO JAMBI

Penduduk di Provinsi Jambi memiliki masalah kesehatan gigi dan mulut sebesar sebesar 37,7%, dan anak usia 10-14 tahun di Indonesia menyikat gigi setiap harinya sebesar 96,5%, dan yang menyikat gigi dengan waktu yang tepat hanya 1,4 %.  Tujuan pengabdian masyarakat edukasi pemeliharaan kesehatan gigi danmulut pada murid SDN 23/IX dan SDN 56/IX Desa Pondok Meja Kecamatan Mestong Jambi. Metode Kegiatan pengabdian kepada masyarakat yaitu metode penyuluhan dan demonstrasi, untuk mengedukasi pemeliharaan kesehatan gigi dan mulut dan keterampilan cara menyikat gigi pada murid kelas IV SDN 23/IX dan SDN 56/IX Desa Pondok Meja Kecamatan Mestong Kab. Muaro Jambi yang berjumlah 86 orang.  pengetahuan pemeliharaan kesehatan gigi dan mulut pada murid kelas IV SDN 23/IX dan SDN 56 Desa Pondok Meja Kecamatan Mestong Kabupaten Muaro Jambi dari 38,1% menjadi 90,5% dan dapat meningkatkan keterampilan menyikat gigi dari yang mampu 20% menjadi mampu 80%. Luaran lainnya adalah artikel jurnal untuk publikasi kegiatan pengabdian masyarakat. Kata Kunci : Pengetahuan, keterampilan menyikat gigi, media vide

HUBUNGAN TINGKAT PENGETAHUAN DENGAN PERILAKU PEMELIHARAAN KESEHATAN GIGI DAN MULUT MURID SEKOLAH DASAR

One of the causes of oral problems is the oral hygiene neglect. Despite the high knowledge in oral health care, lot of children still unable to improve their attitude and practice towards oral health care. The aim of this study is to define the association of knowledge level with behaviour towards oral health care in students of SDN 36/IV Jambi in 2018. This is a cross sectional study comprised of 59 subjects recruited by total sampling method. Data collected from questionnaire filled by subjects followed by chi square analysis. The high, moderate and low knowledge level of SDN 36/IV Jambi students were 66,1%, 32,2% and 1,7% respectively. 67,8% subjects had good behaviour towards oral health care, while the other 32,2% had moderate behaviour. No significant association found between knowledge level and behaviour towards oral health care in students of SDN 36/IV Jambi in 2018 (sig=0,446; p ˂ 0,05)

Pendidikan Politik Pada Generasi Muda Di Pesantren Baitul Arqam Kecamatan Suka Makmur Aceh Besar

Undang-undang nomor 2 tahun 2011 tentang perubahan atas Undang-undang nomor 2 tahun 2008 tentang Partai Politik menyatakan “Pendidikan politik adalah proses pembelajaran tentang hak, kewajiban dan tanggung jawab setiap warga negara dalam kehidupan berbangsa dan bernegara. Politik sering diartikan sebagai seni atau ilmu untuk meraih kekuasaan, pada pratiknya banyak timbul penyimpangan-penyimpangan dalam rangka memperoleh kekuasaan itu sendiri. Pemuda sebagai generasi penerus yang akan memegang tampuk kepemimpinan kedepan, tentu harus melek politik, tidak hanya politik praktis, tetapi juga menentukan arah kemajuan negara. Tujuan dilakukanya pengabdian masyarakat ini untuk memberikan pemahaman kepada siswa di Sekolah Madrasah Aliyah Swasta, Pesantren Baitul Arqam Sibreh tentang pendidikan politik, pemahaman bagi siswa Sekolah Madrasah Aliyah Swasta, di Pesantren Baitul Arqam Sibreh tentang politik. Hasil yang dicapai dalam kegiatan pengabdian masyarakat adalah menunjukan peningkatan pengetahuan dan pemahaman, ditandai dengan partisipasi peserta yang mengajukan pertanyaan sesuai dengan materi penyuluhan hukum. Diharapkan dapat menumbuhkan pengetahuan peserta dan dapat berpartisipasi secara aktif dalam penggunaan hak politik baik bagi sendiri maupun orang lain.  Subtansi pemuda sebagai pemilih pemula tidak menjadi objek politik tetapi juga berperan sebagai subjek politik.  Disarankan kepada peserta penyuluhan agar menindak lanjuti aspek pengetahuan dan sikap untuk diperaktikan dalam kehidupan sehari-hari  berkaitan dengan pendidikan politik. Kegiatan penyuluhan hukum kepada santri dan santriwati Pesantren Darul Arqam Kecamatan Suka Makmur Aceh Besar, seharusnya dilakukan bersama instansi terkait dengan materi-materi hukum lain, sehingga dapat tercapi tujuan yang ingin di inginkan..Kata kunci : Pendidikan Politik, Pemuda, Pesanteren.ABSTRACTThe Law Number 2 Year 2011 amandment of The Law Number 2 Year 2008 about Political Party saysthat, ‘ Political education is a learning process about rights, obligations and responsibility every citizens in the life of the people and of the nation. Politics commonly interpreted as art or knowledge to gain power, in fact there are distortions in order to gain it power. Youths as next generation who will hold the reins of power in the future, surely have to open their eyes on politics, not only political practice, but also determine the direction of the country. The goal of this community service was to educate students in Baitul Arqam Senior High School Sibreh about political education. The results achieved in community service activities are showing an increase in knowledge and understanding, seen at the participation of participants who asked questions in accordance with the legal counseling material. It is hoped that it will increase the knowledge of participants and be able to participate actively in the use of political rights, both for themselves and for others. The substance of youth as novice voters does not become a political object but also acts as a political subject. It is suggested to the counseling participants to follow up on aspects of knowledge and attitudes to be practiced in dailylife related to political education. Legal counseling activities for students of the Darul Arqam Islamic Boarding School, Suka Makmur District, Aceh Besar, should be carried out together with related agencies with other legal materials, so that the goals to be achieved can be achieved.Keywords: Political Education, Youth, Boarding Schoo

Epitheliod Leiomyoma of the Bladder: An Unusual Case of Irritative and Obstructive Voiding Symptoms

Epitheloid leiomyoma is a very rare subtype of benign mesothelial tumors of the bladder. A 46-year-old female patient presented to our hospital with prolonged dysuria, frequency, and recurrent urinary tract infections. Bimanual examination revealed a mobile, round mass in bladder. There was a round hyperdense intravesical mass near bladder neck in computed tomography (CT) scan that was compatible with her magnetic resonance imaging (MRI). A well defined 3 × 4 centimeter mass was seen in superolateral part of bladder neck during cystoscopy. The patient underwent partial cystectomy and histopathologic findings confirmed the diagnosis of epithelioid leiomyoma. The patient's followup was uneventful in a period of 2 years. Size and anatomic location of this tumor were major factors that affect on treatment

Experimental and Computational Observations of Immunogenic Cobalt Porphyrin Lipid Bilayers: Nanodomain-Enhanced Antigen Association.

Cobalt porphyrin phospholipid (CoPoP) can incorporate within bilayers to enable non-covalent surface-display of antigens on liposomes by mixing with proteins bearing a polyhistidine tag (his-tag); however, the mechanisms for how this occurs are poorly understood. These were investigated using the his-tagged model antigen Pfs25, a protein antigen candidate for malaria transmission-blocking vaccines. Pfs25 was found to associate with the small molecule aquocobalamin, a form of vitamin B12 and a cobalt-containing corrin macrocycle, but without particle formation, enabling comparative assessment. Relative to CoPoP liposomes, binding and serum stability studies indicated a weaker association of Pfs25 to aquocobalamin or cobalt nitrilotriacetic acid (Co-NTA) liposomes, which have cobalt displayed in the aqueous phase on lipid headgroups. Antigen internalization by macrophages was enhanced with Pfs25 bound to CoPoP liposomes. Immunization in mice with Pfs25 bound to CoPoP liposomes elicited antibodies that recognized ookinetes and showed transmission-reducing activity. To explore the physical mechanisms involved, we employed molecular dynamics (MD) simulations of bilayers containing phospholipid, cholesterol, as well as either CoPoP or NTA-functionalized lipids. The results show that the CoPoP-containing bilayer creates nanodomains that allow access for a limited but sufficient amount of water molecules that could be replaced by his-tags due to their favorable free energy properties allowing for stabilization. The position of the metal center within the NTA liposomes was much more exposed to the aqueous environment, which could explain its limited capacity for stabilizing Pfs25. This study illustrates the impact of CoPoP-induced antigen particleization in enhancing vaccine efficacy, and provides molecular insights into the CoPoP bilayer properties that enable this

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication

Role of GTPases in assembly of the bacterial 30S subunit

My PhD thesis aims to improve our understanding of the assembly process of the bacterial ribosome and the overarching goal of my research is to identify steps in this pathway that can be targeted with new molecular probes. Ribosome assembly in actively growing Escherichia Coli involves multiple events including synthesis of 54 ribosomal-proteins and their binding to ribosomal RNAs in a hierarchical manner. Many of these events are assisted by a number of accessory proteins known as assembly factors, making the process extremely fast and efficient. My PhD thesis contributes new knowledge on the precise mechanism by which YjeQ and Era assist the ribosome assembly process. Recent work indicates that both factors assist the late stages of the 30S subunit assembly. To reveal the precise role of YjeQ in the context of the mature 30S subunit, we solved the structure of the 30S subunit in complex with YjeQ by cryo-electron microscopy (Cryo-EM). Our data suggested that in addition to work as a maturation factor, YjeQ also functions as a checkpoint protein testing the proofreading ability of the ribosomal subunit prior to the particle's release into the pool of actively translating ribosomes. This work provides the first example of a bacterial assembly factor that tests a specific translation mechanism of the 30S subunit. To investigate the role of Era, we used quantitative mass spectrometry (qMS), microscale thermophoresis (MST) and cryo-EM techniques. We first determined using cryo-EM the structures of the assembly intermediate that accumulated in E. coli upon depletion of Era at 3.8Å resolution. In addition, we also solved the structure of the mature 30S subunit in complex with Era. We found that Era-depleted cells accumulated three assembly intermediates at different stages of maturation ranging from early stage of maturation to the late stage of 30S subunit with missing density both in the central and 3' minor domains of the 30S subunit. Densities for ribosomal proteins (r-proteins) bS21, bS1, uS3 and uS2 bound to the central domain were also missing from the late stage of 30S subunit. The structure of the 30S subunit in complex with Era demonstrated that binding of this factor reverts the mature subunit to an immature state lacking density for the motifs integrating the decoding center. These results suggest that Era facilitates the proper folding of the platform region of the 30S subunit. In addition, Era may work as a placeholder for some of the late ribosomal proteins and prevent premature association of the 30S subunit with the 50S subunit. Finally, we tested whether YjeQ and Era exert their function in conjunction rather than independently. To this end, we obtained the cryo-EM structure of the 30S subunit in complex with Era and YjeQ at 3.5Å resolution. However, we couldn't observe any density for YjeQ in this structure. Moreover, we demonstrated Era binds to the cavity between head and platform and reverts the structure of the 30S subunit to its immature state. These results suggest that Era and YjeQ works subsequently during assembly. Era binds first to the assembling subunit and assists the folding of the platform region at the same time that it prevents the binding of YjeQ. Once Era is released, YjeQ binds assisting the maturation of the decoding center and testing the fidelity of the subunit before releasing it to the pool of fully active ribosomes. Overall, this thesis provides novel insights into the function of two important GTPases dedicated to the assembly process of the bacterial ribosome.Ma thèse de doctorat vise à améliorer notre compréhension des processus d'assemblage du ribosome bactérien et l'objectif global de mon travail de recherche est d'identifier les étapes de cette voie, qui peut être une cible potentielle pour de nouvelles molécules thérapeutiques. L'assemblage de ribosomes chez Escherichia coli en croissance active implique plusieurs évènements, dont la synthèse de 54 protéines ribosomales, et leur liaison aux ARN ribosomiques de façon hiérarchique. Plusieurs de ces évènements sont assistés par certain nombre de protéines accessoires, les facteurs d'assemblage, qui rendent le processus extrêmement rapide et efficace. Ma thèse de doctorat contribue aux nouvelles connaissances sur le mécanisme précis par lequel YjeQ et Era assistent le processus d'assemblage du ribosome. Des travaux récents indiquent que ces deux facteurs aident les dernières étapes de l'assemblage de la sous-unité 30S. Pour révéler le rôle précis de YjeQ dans le contexte de la sous-unité mature 30S, nous avons résolu la structure de la sous-unité 30S en complexe avec YjeQ, par Cryo-microscopie électronique (Cryo-EM). Nos données révèlent qu'en plus de fonctionner comme un facteur de maturation, YjeQ fonctionne également comme une protéine de contrôle qui teste la capacité de relecture de la sous-unité ribosomale, avant la libération de la particule dans le pool des ribosomes actifs. Ce travail fournit le premier exemple d'unfacteur d'assemblage bactérien qui teste un mécanisme de traduction spécifique de la sous-unité 30S. Pour examiner le rôle d'Era, nous avons utilisé la spectrométrie de masse quantitative (qSM), la thermophorèse à l'échelle microscopique (MST) et la technique de la cryo-EM. Nous avons d'abord déterminé, en utilisant la cryo-EM, les structures de l'intermédiaire d'assemblage accumulées dans E. Coli lors de la déplétion d'Era, à une résolution de 3.8Å. En outre, nous avons également résolu la structure de la sous-unité 30S mature dans le complexe avec l'Era. Nous avons trouvé que les bactéries déplétée en Era accumulaient principalement un intermédiaire d'assemblage avec des densités manquantes à la fois dans les domaines central et 3' mineur de la sous-unité 30S. Les densités des protéines ribosomiques bS21, bS1, uS3 et uS2 liées au domaine central étaient également manquantes. La structure de la sous-unité 30S en complexe avec Era a démontré que la liaison de ce facteur ramène la sous-unité mature à un état immature auquel manquent les densités des motifs intégrant le centre de décodage. Ces résultats suggèrent qu'Era facilite le repliement correct de la région de la plateforme de la sous-unité 30S. En outre, Era peut agir comme un espace réservé pour certaines des protéines ribosomales tardives et empêcher l'association prématurée de la sous-unité 30S avec la sous-unité 50S. Enfin, nous avons testé si YjeQ et Era exerçaient leur fonction en conjonction plutôt qu'indépendamment. À cette fin, nous avons obtenu la structure en Cryo-EM de la sous-unité 30S dans le complexe avec Era et YjeQ à une résolution de 3.8Å. Cependant, nous n'avons pas pu observer de densité pour YjeQ dans cette structure. De plus, nous avons démontré qu'Era se lie à la cavité entre la tête et la plateforme et ramène la structure de la sous-unité 30S à son état immature. Ces résultats suggèrent qu'Era et YjeQ fonctionnent successivement pendant l'assemblage. Era se lie d'abord à la sous-unité d'assemblage et aide au repliement de la région de la plateforme en même temps qu'elle empêche la liaison de YjeQ. Une fois qu'Era est libérée, YjeQ se lie pour aider à la maturation du centre de décodage et tester l'efficacité de la sous-unité avant de la relâcher dans le pool de ribosomes actifs. Dans l'ensemble, cette thèse fournit de nouvelles pistes sur la compréhension de la fonction de deux GTPases importantes impliquées dans le processus d'assemblage du ribosome bactérien