The value of anaesthetic steroids alphaxolonealphadolone in pregnant mice

Thiopentone, pentobarbitone, diazepam and alphaxolone-alphadolone were evaluated as anaesthetic agents in pregnant mice. Incidence of mortality was: Thiopentone 15%, pentobarbitone 13%, diazepam 8%, and alphaxolone-alphadolone 0%. Besides the low mortality, alphaxolone-alphadolone also distinguished by showing no side-effeets at all, neither to the dams nor to the fetuses, and it is recommended as an excellent anaesthetic in pregnant mice

The Non-Perturbative O(g6){\cal O}(g^6) Contribution to the Free Energy of Hot SU(N) Gauge Theory

The non-perturbative input necessary for the determination of the ${\cal O}(g^6)$ part of the weak coupling expansion of the free energy density for SU(2) and SU(3) gauge theories is estimated. Although the perturbative information completing the contribution to this order is missing, we give arguments that the magnetic fluctuations are dominated by screened elementary magnetic gluons.Comment: Talk presented at LATTICE96(finite temperature) 3 pages Latex2e, 3 ps figures, 14 k

Matrix Metalloproteinase 13 Is Induced in Fibroblasts in Polyomavirus Middle T Antigen-Driven Mammary Carcinoma without Influencing Tumor Progression

Matrix metalloproteinase (MMP) 13 (collagenase 3) is an extracellular matrix remodeling enzyme that is induced in myofibroblasts during the earliest invasive stages of human breast carcinoma, suggesting that it is involved in tumor progression. During progression of mammary carcinomas in the polyoma virus middle T oncogene mouse model (MMTV-PyMT), Mmp13 mRNA was strongly upregulated concurrently with the transition to invasive and metastatic carcinomas. As in human tumors, Mmp13 mRNA was found in myofibroblasts of invasive grade II and III carcinomas, but not in benign grade I and II mammary intraepithelial neoplasias. To determine if MMP13 plays a role in tumor progression, we crossed MMTV-PyMT mice with Mmp13 deficient mice. The absence of MMP13 did not influence tumor growth, vascularization, progression to more advanced tumor stages, or metastasis to the lungs, and the absence of MMP13 was not compensated for by expression of other MMPs or tissue inhibitor of metalloproteinases. However, an increased fraction of thin collagen fibrils was identified in MMTV-PyMT;Mmp13−/− compared to MMTV-PyMT;Mmp13+/+ tumors, showing that collagen metabolism was altered in the absence of MMP13. We conclude that the expression pattern of Mmp13 mRNA in myofibroblasts of invasive carcinomas in the MMTV-PyMT breast cancer model recapitulates the expression pattern observed in human breast cancer. Our results suggest that MMP13 is a marker of carcinoma-associated myofibroblasts of invasive carcinoma, even though it does not make a major contribution to tumor progression in the MMTV-PyMT breast cancer model

X- and gamma-ray observations of the 15 November 1991 Solar Flare

This work expands the current understanding of the 15 November 1991 Solar Flare. The flare was a well observed event in radio to gamma-rays and is the first flare to be extensively studied with the benefit of detailed soft and hard X-ray images. In this work, we add data from all four instruments on the Compton Gamma Ray Observatory. Using these data we determined that the accelerated electron spectrum above 170 keV is best fit with a power law with a spectral index of −4.6, while the accelerated proton spectrum above 0.6 MeV is fit with a power law of spectral index −4.5. From this we computed lower limits for the energy content of these particles of∼1023 ergs (electrons) and ∼1027 ergs (ions above 0.6 MeV). These particles do not have enough energy to produce the white-light emission observed from this event. We computed a time constant of 26+20−15 s for the 2.223 MeV neutron capture line, which is consistent at the 2σ level with the lowest values of ∼70 s found for other flares. The mechanism for this short capture time may be better understood after analyses of high energy EGRET data that show potential evidence for pion emission near ∼100 MeV

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre-versus Postmenopausal Women

Background: Climacteric increases the risk of thrombotic events by alteration of plasmatic coagulation. Up to now, less is known about changes in platelet-(PMP) and endothelial cell-derived microparticles (EMP). Methods: In this prospective study, plasma levels of microparticles (MP) were compared in 21 premenopausal and 19 postmenopausal women. Results: No altered numbers of total MP or EMP were measured within the study groups. However, the plasma values of CD61-exposing MP from platelets/megakaryocytes were higher in premenopausal women (5,364 x 10(6)/l, range 4,384-17,167) as compared to postmenopausal women (3,808 x 10(6)/l, range 2,009-8,850; p = 0.020). This differentiation was also significant for the subgroup of premenopausal women without hormonal contraceptives (5,364 x 10(6)/l, range 4,223-15,916; p = 0.047; n = 15). Furthermore, in premenopausal women, higher plasma levels of PMP exposing CD62P were also present as compared to postmenopausal women (288 x 10(6)/l, range 139-462, vs. 121 x 10(6)/l, range 74-284; p = 0.024). This difference was also true for CD63+ PMP levels (281 x 10(6)/l, range 182-551, vs. 137 x 10(6)/l, range 64-432; p = 0.015). Conclusion: Climacteric lowers the level of PMP but has no impact on the number of EMP in women. These data suggest that PMP and EMP do not play a significant role in enhancing the risk of thrombotic events in healthy, postmenopausal women. Copyright (C) 2012 S. Karger AG, Base

Universality of the 1/3 shot-noise suppression factor in nondegenerate diffusive conductors

Shot-noise suppression is investigated in nondegenerate diffusive conductors by means of an ensemble Monte Carlo simulator. The universal 1/3 suppression value is obtained when transport occurs under elastic collision regime provided the following conditions are satisfied: (i) The applied voltage is much larger than the thermal value; (ii) the length of the device is much longer than both the elastic mean free path and the Debye length. By fully suppressing carrier-number fluctuations, long range Coulomb interaction is essential to obtain the 1/3 value in the low-frequency limit.Comment: RevTex, 4 pages, 4 figure

COMPTEL’s solar flare catalog

COMPTEL, the imaging gamma‐ray telescope, capable of detecting gamma rays in the range of 0.1–30 MeV, is one of four instruments aboard NASA’s Compton Gamma‐Ray Observatory. The Comptel burst detectors (single Defector Mode) have a field of view of ∼2.5 π sr. These detectors of COMPTEL permit measurements of energy spectra and time histories of solar flare gamma‐ray emission. A search through the Single Detector Mode’s data is being conducted. We summarize the preliminary results of this search

Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

BackgroundDespite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear.MethodsIn this study the upregulation of TF release upon activation of various cancer cell lines, and the correlation with TF and PAR2 expression and/or activity was examined. Microvesicle release was induced by PAR2 activation in seventeen cell lines and released microvesicle density, microvesicle-associated TF activity, and phoshpatidylserine-mediated activity were measured. The time-course for TF release was monitored over 90 min in each cell line. In addition, TF mRNA expression, cellular TF protein and cell-surface TF activities were quantified. Moreover, the relative expression of PAR2 mRNA and cellular protein were analysed. Any correlations between the above parameters were examined by determining the Pearson’s correlation coefficients.ResultsTF release as microvesicles peaked between 30–60 min post-activation in the majority of cell lines tested. The magnitude of the maximal TF release positively correlated with TF mRNA (c = 0.717; p

Evidence for a stem cell hierarchy in the adult human breast

Cellular pathways that contribute to adult human mammary gland architecture and lineages have not been previously described. In this study, we identify a candidate stem cell niche in ducts and zones containing progenitor cells in lobules. Putative stem cells residing in ducts were essentially quiescent, whereas the progenitor cells in the lobules were more likely to be actively dividing. Cells from ducts and lobules collected under the microscope were functionally characterized by colony formation on tissue culture plastic, mammosphere formation in suspension culture, and morphogenesis in laminin-rich extracellular matrix gels. Staining for the lineage markers keratins K14 and K19 further revealed multipotent cells in the stem cell zone and three lineage-restricted cell types outside this zone. Multiparameter cell sorting and functional characterization with reference to anatomical sites in situ confirmed this pattern. The proposal that the four cell types are indeed constituents of an as of yet undescribed stem cell hierarchy was assessed in long-term cultures in which senescence was bypassed. These findings identify an adult human breast ductal stem cell activity and its earliest descendants

Gauge Boson Masses in the 3-d, SU(2) Gauge-Higgs Model

We study gauge boson propagators in the symmetric and symmetry broken phases of the 3-d, $SU(2)$ gauge-Higgs model. Correlation functions for the gauge fields are calculated in Landau gauge. They are found to decay exponentially at large distances leading to a non-vanishing mass for the gauge bosons. We find that the W-boson screening mass drops in the symmetry broken phase when approaching the critical temperature. In the symmetric phase the screening mass stays small and is independent of the scalar--gauge coupling (the hopping parameter). Numerical results coincide with corresponding calculations performed for the pure gauge theory. We find $m_w = 0.35(1)g^2T$ in this phase which is consistent with analytic calculations based on gap equations. This is, however, significantly smaller than masses extracted from gauge invariant vector boson correlation functions. As internal consistency check we also have calculated correlation functions for gauge invariant operators leading to scalar and vector boson masses. Finite lattice size effects have been systematically analyzed on lattices of size $L^2\times L_z$ with $L=4-24$ and $L_z = 16 - 128$.Comment: 20 pages, LaTeX2e File, 8 Postscript figure