Untersuchung der vaskulären Blutflussregulation und der Sauerstoffsättigung retinaler Gefäße sowie der Autofluoreszenzeigenschaften des Augenhintergrundes bei Glaukom

Ziel der Arbeit war die Untersuchung der Sauerstoffversorgung der Retina bei primärem Offenwinkelglaukom (POWG). Die Durchmesser und die Sauerstoffsättigung peripapillärer Gefäße wurden bei 41 Patienten und bei 40 gesunden Probanden erhoben. Mittels einer modifizierten Funduskamera entstanden Aufnahmen zur Bestimmung der Gefäßdurchmesser. Die Ermittlung der Sauerstoffsättigung basierte auf den verschiedenen Absorptionsspektren oxygenierten und desoxygenierten Hämoglobins. Nach der Erhebung in Ruhe wurde eine Flickerlichtstimulation durchgeführt. Bei POWG zeigten sich eine Erhöhung der venösen Sauerstoffsättigung sowie eine Abnahme der arteriovenösen Sättigungsdifferenz in Ruhe. Unter Stimulation lagen eine geringere venöse Durchmesserzunahme sowie eine verminderte Änderung der venösen Sättigung und der arteriovenösen Differenz vor. Die optische Kohärenztomographie zeigte eine Reduktion der Dicke der retinalen Nervenfaserschicht (RNFS) bei POWG. In beiden Studiengruppen korrelierten die Durchmesser in Ruhe mit der RNFS-Dicke. Die Krankheitsschwere wurde mittels Perimetrie eingeteilt. Bei POWG lag eine Abnahme des arteriellen Gefäßdurchmessers mit zunehmender Schädigung vor. Unter Einsatz eines modifizierten Laser-Scanning-Ophthalmoskopes erfolgte erstmals das Fluorescence Lifetime Imaging bei POWG. Die Ergebnisse von 43 Patienten wurden mit denen 54 Gesunder verglichen. Signifikante Unterschiede zeigten sich bei Untersuchungen mit natürlicher Linse, nicht jedoch bei künstlicher Intraokularlinse. Zeichen einer retinalen Mangelversorgung konnten nicht gefunden werden. Die reduzierte vaskuläre Reaktion auf Flickerlicht ist potentiell durch eine Veränderung der neurovaskulären Kopplung bei POWG bedingt. Ursache der erhöhten venösen Sauerstoffsättigung und der reduzierten arteriovenösen Differenz könnte ein verminderter Sauerstoffbedarf sein. Die Abnahme des arteriellen Durchmessers mit steigendem Gesichtsfeldausfall und mit sinkender Dicke der RNFS unterstützt diese Annahme

Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera: Blissidae)

The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae

Probing the relevance of the hippocampus for conflict-induced memory improvement

The hippocampus plays a key role for episodic memory. In addition, a small but growing number of studies has shown that it also contributes to the resolution of response conflicts. It is less clear how these two functions are related, and how they are affected by hippocampal lesions in patients with mesial temporal lobe epilepsy (MTLE). Previous studies suggested that conflict stimuli might be better remembered, but whether the hippocampus is critical for supporting this interaction between conflict processing and memory formation is unknown. Here, we tested 19 patients with MTLE due to hippocampal sclerosis and 19 matched healthy controls. Participants performed a face-word Stroop task during functional magnetic resonance imaging (fMRI) followed by a recognition task for the faces. We tested whether memory performance and activity in brain regions implicated in long-term memory were modulated by conflict during encoding, and whether this differed between MTLE patients and controls. In controls, we largely replicated previous findings of improved memory for conflict stimuli. While MTLE patients showed response time slowing during conflict trials as well, they did not exhibit a memory benefit. In controls, neural activity of conflict resolution and memory encoding interacted within a hippocampal region of interest. Here, left hippocampal recruitment was less efficient for memory performance in incongruent trials than in congruent trials, suggesting an intrahippocampal competition for limited resources. They also showed an involvement of precuneus and posterior cingulate cortex during conflict resolution. Both effects were not observed in MTLE patients, where activation of the precuneus and posterior cingulate cortex instead predicted later memory. Further research is needed to find out whether our findings reflect widespread functional reorganization of the episodic memory network due to hippocampal dysfunction

Morphology and Proteome Characterization of the Salivary Glands of the Western Chinch Bug (Hemiptera: Blissidae)

The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae

Analysis of Male Pheromones That Accelerate Female Reproductive Organ Development

Male odors can influence a female's reproductive physiology. In the mouse, the odor of male urine results in an early onset of female puberty. Several volatile and protein pheromones have previously been reported to each account for this bioactivity. Here we bioassay inbred BALB/cJ females to study pheromone-accelerated uterine growth, a developmental hallmark of puberty. We evaluate the response of wild-type and mutant mice lacking a specialized sensory transduction channel, TrpC2, and find TrpC2 function to be necessary for pheromone-mediated uterine growth. We analyze the relative effectiveness of pheromones previously identified to accelerate puberty through direct bioassay and find none to significantly accelerate uterine growth in BALB/cJ females. Complementary to this analysis, we have devised a strategy of partial purification of the uterine growth bioactivity from male urine and applied it to purify bioactivity from three different laboratory strains. The biochemical characteristics of the active fraction of all three strains are inconsistent with that of previously known pheromones. When directly analyzed, we are unable to detect previously known pheromones in urine fractions that generate uterine growth. Our analysis indicates that pheromones emitted by males to advance female puberty remain to be identified

Don’t mind if I do: Arctic humpback whales respond to winter foraging opportunities before migration

Migration patterns are fundamentally linked to the spatio-temporal distributions of prey. How migrating animals can respond to changes in their prey's distribution and abundance remains largely unclear. During the last decade, humpback whales (Megaptera novaeangliae) used specific winter foraging sites in fjords of northern Norway, outside of their main summer foraging season, to feed on herring that started overwintering in the area. We used photographic matching to show that whales sighted during summer in the Barents Sea foraged in northern Norway from late October to February, staying up to three months and showing high inter-annual return rates (up to 82%). The number of identified whales in northern Norway totalled 866 individuals by 2019. Genetic sexing and hormone profiling in both areas demonstrate a female bias in northern Norway and suggest higher proportions of pregnancy in northern Norway. This may indicate that the fjord-based winter feeding is important for pregnant females before migration. Our results suggest that humpback whales can respond to foraging opportunities along their migration pathways, in some cases by continuing their feeding season well into winter. This provides an important reminder to implement dynamic ecosystem management that can account for changes in the spatio-temporal distribution of migrating marine mammals